培养基对海藻真菌PT2菌株的生长及活性代谢产物合成的影响   总被引：3，自引：0，他引：3  

彭琛  郑忠辉  杜希萍  黄耀坚  宋思扬  苏文金 《台湾海峡》2005,24(1):90-96

本文采用PD、GPY、YES、SYE、GPM5种不同培养基培养海藻真菌菌株PT2，并对其在不同培养基中的生长情况、代谢产物的形成及抗肿瘤活性进行分析，旨在了解不同培养基对菌株生长和活性物质合成的影响．结果显示，PT2菌株在不同培养基上的培养特征及生长速率有所不同，液体培养基发酵液抽提物的抗肿瘤活性也有较大差别，其中SYE发酵液抽提物活性最强，对KB、Raji和Hela 3种肿瘤细胞的IC50分别为7．6μg／cm^3、2．4μg／cm^3和3．3μg／cm^3．采用HPLC法对不同抽提物进行分析，发现它们之间的组成成分有显著差异．本文的实验结果表明，不同培养基对该菌株的生长及活性代谢产物的合成具有显著的影响。  相似文献   

温度对美国硬壳蛤滤食率、耗氧率和排氨率的影响   总被引：8，自引：0，他引：8  

柴雪良  方军  林志华  张炯明  单乐州 《海洋科学》2005,29(8):33-36

在实验条件下，采用静水方法，测定了水温11．5、22．5、28．0、32．0℃下，硬壳蛤的滤食率、耗氧率和排泄率。结果表明，在11，5～22．5℃-之间，硬壳蛤的上述生理指标随温度的升高而增加，差异显著。而在22．5、28．0、32．0℃之间，生理指标的差异并不十分显著，间接说明了硬壳蛤对温度的较强适应能力，适合在浙江省等东南沿海进行推广养殖。  相似文献   

影响萼花臂尾轮虫种群增长的生态学因子的研究：温度和饵料密度的影响   总被引：11，自引：0，他引：11  

王金秋 《海洋湖沼通报》1995,(4):21-27

本文研究了在5个温度梯度和4个饵料密度的20个试验组合下，萼化臂尾轮虫（Brachionuscalyciflorus）的种群增长规律。结果表明，该轮虫实验种群的最适温度范围是28—32℃，临界高温是36－40℃；最适饵料密度是45－65×106个／ml。这两个生态因子的最佳组合是温度32℃，饵料密度为65×106个／ml，此时，种群达最大密度3．4×103个／ml。  相似文献   

珠江口河流输沙、河口沉积与粒度信息之间的联系   总被引：2，自引：0，他引：2  

贾建军  高抒  高建华  潘少明  王爱军 《海洋科学进展》2005,23(3):297-304

河口三角洲地区泥沙供应充分，是现代沉积速率较高的区域。快速沉积的泥沙包含高分辨率的环境信息。采用放射性同位素测年方法研究了珠江口伶仃洋大铲湾海域6个柱样的现代沉积速率，以2mm的间隔分析了其中2个柱样上部的粒度，并对其平均粒径和珠江流域的年输沙量进行了快速傅里叶变换，以期获取河流泥沙供应、河口沉积速率和沉积物垂向粒度变化的对应关系。结果表明，大铲湾海域的沉积速率为1～3cm／a，受动力条件和泥沙供应条件的影响显著。2个柱样的平均粒径的最显著重现周期与珠江年输沙量的周期性有较好的对应关系，并可能反映了季节的、年的和多年的沉积速率信息。对于每年沉积厚度在厘米级的环境，以毫米级的高分辨率进行粒度分析，有可能揭示出沉积物供应的季节变化和年际变化。本研究亦可为其他河口的相关工作提供借鉴。  相似文献   

Biocorrosion by Bivalves   总被引：2，自引：0，他引：2  

Karl  Kleemann 《Marine Ecology》1996,17(1-3):145-158

Abstract. A survey of the historical background of chemically boring bivalves and the proposed methods of boring, indications for biocorrosion, observations, and experimental results are provided. The regional impact to the ecosystem is discussed with examples from the N. Adriatic, Caribbean, and E. Pacific. The fossil record of the geologically oldest biocorroders extends back into the Mesozoic, i.e ., U. Triassic for Lithophaga and Jurassic for gastrochaenids.  相似文献   

Estimating migration rates from two tag-release/one recovery experiments   总被引：1，自引：0，他引：1  

Shirakihara  Kunio; Kitada  Shuichi 《ICES Journal of Marine Science》2004,61(5):821-828

  相似文献   

Probing molecular-level transformations of dissolved organic matter: insights on photochemical degradation and protozoan modification of DOM from electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry     

Elizabeth B. Kujawinski  Rossana Del Vecchio  Neil V. Blough  Geoffrey C. Klein  Alan G. Marshall   《Marine Chemistry》2004,92(1-4):23

Molecular-level characterization of natural organic matter (NOM) has been elusive due to the inherent complexity of natural organic mixtures and to the fact that individual components are often polar and macromolecular. Electrospray ionization (ESI) is a “soft” ionization technique that ionizes polar compounds from aqueous solution prior to injection into a mass spectrometer. The highest resolution and mass accuracy of compounds within NOM have been achieved when ESI is combined with an ultrahigh-resolution mass spectrometer such as the Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS). With this technique, individual molecules within a variety of natural organic mixtures can be detected and their elemental composition can be determined. At low mass-to-charge (m/z) ratio, the resolution is high enough to assign exact molecular formulas allowing specific components of these mixtures to be identified. In addition to molecular identification, we can now use ESI FT-ICR MS to examine molecular-level changes in different organic mixtures as a function of relevant geo-processes, such as microbial alterations and photochemistry. Here we present the results from the application of ESI FT-ICR MS to two geochemical questions: (1) the effect of photoirradiation on the molecular composition of fulvic acids and (2) the role of protozoan grazers in the modification of DOM in aquatic systems.  相似文献   

一维扩散方程整体观测资料下的初值变分同化反演     

张瑰 《海洋预报》2006,23(Z1):34-41

本文考虑一维扩散方程的反问题,利用变分同化方法通过观测资料来确定方程中的未知初值,通过分析观测误差对于初值误差的影响,证明变分同化初值收敛于原问题的真实参数,并得到了参数的收敛精度。同时将得到的初值代入预报模式中,得到预报解,并分析了预报解的收敛性和预报误差。  相似文献   

Community Structure and Dynamics of Phytoplankton in the Western Subarctic Pacific Ocean: A Synthesis   总被引：1，自引：1，他引：1  

Hongbin Liu  Koji Suzuki  Hiroaki Saito 《Journal of Oceanography》2004,60(1):119-137

The phytoplankton community in the western subarctic Pacific (WSP) is composed mostly of pico- and nanophytoplankton. Chlorophyll a (Chl a) in the <2 μm size fraction accounted for more than half of the total Chl a in all seasons, with higher contributions of up to 75% of the total Chl a in summer and fall. The exception is the western boundary along the Kamchatka Peninsula and Kuril Islands and the Oyashio region where diatoms make up the majority of total Chl a during the spring bloom. Among the picophytoplankton, picoeukaryotes and Synechococcus are approximately equally abundant, but the former is more important in term of carbon biomass. Despite the lack of a clear seasonal variation in Chl a concentration, primary productivity showed a large seasonal variation, and was lowest in winter and highest in spring. Seasonal succession in the phytoplankton community is also evident with the abundance of diatoms peaking in May, followed by picoeukaryotes and Synechococcus in summer. The growth of phytoplankton (especially >10 μm cell size) in the western subarctic Pacific is often limited by iron bioavailability, and microzooplankton grazing keeps the standing stock of pico- and nano-phytoplankton low. Compared to the other HNLC regions (the eastern equatorial Pacific, the Southern Ocean, and the eastern subarctic Pacific), iron limitation in the Western Subarctic Gyre (WSG) may be less severe probably due to higher iron concentrations. The Oyashio region has similar physical condition, macronutrient supply and phytoplankton species compositions to the WSG, but much higher phytoplankton biomass and primary productivity. The difference between the Oyashio region and the WSG is also believed to be the results of difference in iron bioavailability in both regions. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

黄茅海河口湾现代动力地貌体系和冲淤过程分析     

杨雪舞  于红兵  孙宗勋  梁添明 《热带海洋学报》1997,(1)

1980—1993年对黄茅海河口湾进行沉积物采样和水流测定及水深测量。根据水动力和地形条件，冲淤分析及Mclaren模型研究河口湾的动力地貌体系、冲淤特征和现代沉积物运移。结果表明：（1）水下地形主要为下泄流或上溯流控制的“深槽－槽沟－浅滩－湾口”的动力地貌体系，反映了河口湾“东进西出”的水流格局；（2）整个河口湾以淤积为主，只有崖门深槽有较明显的优势冲刷特征，并随着崖门深槽向海推移和河口湾“东进西出”水动力作用，黄茅海落潮三角洲相应向西南进积；（3）应用Mclaren模型揭示了黄茅海河口湾现代沉积物运移规律，同样反映了河口湾具有“东进西出”的运移趋势。  相似文献