Modified sublimation to isolate phenanthrene-degrading bacteria of the genera Sphingomonas and Burkholderia from Xiamen oil port     

X. Huang  Y. Tian  Y.R. Luo  H.J. Liu  Wei Zheng  T.L. Zheng   《Marine pollution bulletin》2008,57(6-12):538

Sublimation was developed by Alley and Brown (2000) in order to isolate bacterial strains that were capable of degrading water insoluble compounds. In this study, sublimation was modified by the use of nutritional agar plates, instead of mineral salt agar, to isolate phenanthrene-degrading bacteria from a mixed culture that had been enriched under the selective pressure of high phenanthrene content. Five strains were obtained with different morphology and degradation ability. Based on the 16S rDNA sequence, two of them were classified as species of the genus Sphingomonas; the others as species of the genus Burkholderia. Denaturing gradient gel electrophoresis (DGGE) was introduced to detect dynamic changes in the bacterial community during enrichment batch culture, and to determine any correlation between the five isolates and the phenanthrene-degrading consortium. The DGGE profile indicated that these five isolates corresponded to four dominant bands of the consortium. Compared to traditional means of isolation, we concluded that modified sublimation is effective and more convenient.  相似文献   

中国自然铜矿床类型、特征、分布及形成条件   总被引：2，自引：0，他引：2  

曾乔松  陈广浩  王核 《地质科技情报》2006,25(6):41-46

近年来的找矿进展显示,我国至少存在2种以上成因类型的自然铜矿床(矿化带):火山-沉积碎屑岩型和陆相砂页岩型等,其主要分布在扬子地块西南缘和新疆天山地区.以湘西九曲湾(麻阳)铜矿和滇东北沿河铜矿为例,结合国外典型自然铜矿床(矿化带),分析了自然铜的成矿学特征及形成条件.通过对比分析沉积砂岩铜矿(硫化物)与自然铜矿的成矿学特征,认为自然铜成矿必须具备3个条件:①孔隙度丰富而适宜的储矿岩石;②水-岩反应中有利于含铜岩石的萃取和运移的浅成低温热液(卤水);③贫硫富生物有机质的地球化学还原障壁带.研究显示,自然铜形成于碱性贫硫的还原环境中,生物有机质对自然铜的成矿起重要作用,相对封闭的成矿环境、独特的流体性质对自然铜起保护作用.我国南方的自然铜矿床一般显示出层控、浅成低温及生物有机质参与成矿等特点.最后探讨了自然铜矿床在我国的找矿勘探前景.  相似文献   

Absorption of Arsenite on Several Iron （Hydro-）Oxides and Impact from Pre-processing Methods     

YE Ying JI Shanshan WU Daidai LI Jun ZHANG Weirui 《《地质学报》英文版》2006,80(2):200-205

The absorption reactions of arsenite on Fe （hydro-）oxides are studied. The three absorbent types are Fe（OH）3 gel and two Fe （hydro-）oxides, in which the Fe（OH）3 gel was dried in a microwave oven under vacuum at 80℃. It is found that pH changes from 9.71 to 10.36 in 6 minutes after the Fe （OH）3 gel was mixed with NaAsO2 solution, as the arsenite replaces the OH- in goethite and Fe（OH）3. At the 40th minute after the start of the reaction, pH decreases, which is most probably because that the monodentate surface complex of absorbed arsenite has changed into mononuclear-bidentate complex and released proton. The decline in pH values indicates not the end of the absorption but a change in the reaction type. Temperature and dissolved gas has little effect on these two types of reactions. The total absorption of arsenite increases after the absorbent is irradiated with ultrasound, which also lead to difficulty in separating the solids from solution. The absorption capacity for arsenite of Fe（OH）3 gel dried in a microwave oven under vacuum is 53.18% and 17.22% respectively better than that of Fe （OH）3 gel and gel dried at 80℃. The possible reasons are that the water molecules in the gel vibrates with high frequency under the effect of microwave irradiation, thereby producing higher porosity and improved surface activity.  相似文献   

Diversity, Distribution Pattern and Conservation Status of the Plants Used in Liver Diseases/Ailments in Indian Himalayan Region     

S.S. Samant Shreekar Pant 《山地科学学报》2006,3(1):28-47

Introduction The Indian Himalayan Region (IHR) is known for its unique, natural, representative biodiversity (Samant et al. 1998a). This biodiversity is being utilized by the inhabitants of the IHR in various forms, i.e., medicine, food (wild edibles), fuel, fodder, timber, making agricultural tools, fiber, religious and various other purposes (Samant and Dhar 1997, Samant et al. 1998b). In the remote areas of the IHR where primary health centers are located far from the villages, the in…  相似文献   

Interaction of Freshly Precipitated Silica Gel with Aqueous Silicic Acid Solutions under Ambient and Near Neutral pH-conditions: A Detailed Analysis of Linear Rate Law     

Jiří Faimon  Martin Blecha 《Aquatic Geochemistry》2008,14(1):1-40

Interaction of freshly precipitated silica gel with aqueous solutions was studied at laboratory batch experiments under ambient and near neutral pH-conditions. The overall process showed excellent reversibility: gel growth could be considered as an opposite process to dissolution and a linear rate law could be applied to experimental data. Depending on the used rate law form, the resulting rate constants were sensitive to errors in parameters/variables such as gel surface area, equilibrium constants, Si-fluxes, and reaction quotients. The application of an Integrated Exponential Model appeared to be the best approach for dissolution data evaluation. It yielded the rate constants k _dissol ∼ (4.50 ± 0.68) × 10⁻¹² and k _growth ∼ (2.58 ± 0.39) × 10⁻⁹ mol m⁻² s⁻¹ for zero ionic strength. In contrast, a Differential Model gave best results for growth data modeling. It yielded the rate constants k _dissol ∼ (1.14 ± 0.44) × 10⁻¹¹ and k _growth ∼ (6.08 ± 2.37) × 10⁻⁹ mol m⁻² s⁻¹ for higher ionic strength (I ∼ 0.04 to 0.11 mol L⁻¹). The found silica gel solubility at zero ionic strength was somewhat lower than the generally accepted value. Based on the and standard Gibbs free energy of silica gel formation was calculated as and −850,318 ± 20 J mol⁻¹, respectively. Activation energies for silica gel dissolution and growth were determined as and respectively. An universal value for growth of any silica polymorph, is not consistent with the value for silica gel growth, which questions the hypothesis about one unique activated complex controlling the silica polymorph growth.  相似文献   

Comparative proteome analysis of butachlor-degrading bacteria   总被引：1，自引：0，他引：1  

Jinhua Wang  Yitong Lu  Yingying Chen 《Environmental Geology》2008,53(6):1339-1344

A Pseudomonas putida strain, named ER1, was isolated from an agricultural soil and found to actively degrade the herbicide butachlor. The enzyme extracted from ER1 could degrade butachlor. Furthermore, incubation of ER1 in a medium containing 50 mg/kg of butachlor after 3 days resulted in the high butachlor-degrading enzyme activity of ER1. Response of ER1 to butachlor might be related to changes in protein composition at both quantitative and qualitative levels. Total proteins were extracted from control strain (incubated in the medium without butachlor) and the treated strain (incubated in the medium with butachlor). The proteins were separated by two-dimensional gel electrophoresis. Of the total number of ER1 protein, 11 spots were significantly changed under butachlor stress. Analysis by matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry coupled with database searching allowed the function of some proteins which were similar to the hydrolases activity or oxidoreductase activity.  相似文献   

成矿流体中碲逸度对自然金成色的影响   总被引：1，自引：0，他引：1  

张招崇 《黄金地质》1997,3(4):9-13

富碲化物型金矿具有自然金成色高的特点，通过热力学分析，推导出碲逸度与温度和自然金中银含量的关系式，建立了ｆＴｅ２－Ｔ－ＮＡｇ图解，并论述了该类型金矿中高自然金成色的内在原因，即成矿溶液中高的碲逸度导致了高的自然金成色，同时也分析了溶液中碲逸度对金银矿物的共生组合的影响，以及对如何计算成矿溶液中碲逸度问题进行了讨论。  相似文献   

河南祁雨沟金矿床冰长石中自然铜超显微包体的发现及成因   总被引：1，自引：1，他引：1  

栾文楼 《矿物学报》1991,11(3):281-284,F001

经透射电镜研究,在祁雨沟金矿床的冰长石中发现有自然铜超显微包体。作者认为这种超显微包体是在低fs_2、fo-2、富Cl的条件下形成,Cu是以CuCl-2~-络合物形式搬运。晶出的自然铜被正在结晶的冰长石捕获,使其封闭起来,未受到后来富fs_2流体的作用。  相似文献   

吉林海沟金矿床自然金的矿物学特征   总被引：1，自引：0，他引：1  

陈殿芬  孙淑琼 《地质论评》1992,38(5)

海沟金矿床是一个大型的沉积变质—热液矿床。自然金是矿床主要的金矿物,它主要富集于自然金—硫化物阶段。自然金的晶形多呈各种形态的显微金分布于硫化物、碲化物及石英的粒间或裂隙中。它的比重大,其晶胞参数与纯金接近。金的成色高并且变化小,成色的高低与矿物组合有关,但矿化深度和温度对它的影响不大。金的颜色指数随成色的变化而变化。自然金的矿物学特征与矿床成因类型有密切关系。  相似文献   

无固相弱凝胶钻井液在水井施工中的应用          下载免费PDF全文

吴跃钢  徐菁 《探矿工程》2016,43(10):261-264

结合水井施工实例,开展了对无固相弱凝胶钻井液及生物酶破胶剂的应用研究。弱凝胶钻井液具有独特的流变性,动塑比高、低剪切速率粘度高,有很好的携砂能力,除具备冷却钻头、清除孔底岩屑、护壁防坍功能外,还具有无固相,不易堵塞地层孔隙,减少钻井液中固相对含水层的伤害的特点。加入生物酶破胶剂后,能清除在井壁形成的泥皮,疏通水流通道,取得了单井出水量和渗透系数有较大增加的应用效果,对在水井施工中加强含水层的保护提供了新的方法。  相似文献