Isolation and characterization of polymorphic microsatellite loci in large yellow croaker, Larimichthys crocea     

YE Hu  REN Peng  ZHAO Guangtai  YUE Genhu  WANG Zhiyong 《海洋学报(英文版)》2012,31(4):149-153

An (AC)n-microsatellite-enriched library for Larimichthys crocea was constructed in this study. Primers for fifty simple sequence repeat (SSR) loci were synthesized and genotyped on 30 L. crocea individuals from Guanjingyang wild population (WP) in Fujian Province and 38 individuals from Ningbo cultured population (CP) in Zhejiang Province. Only 21 loci were successfully amplified and polymorphic in two populations. In WP, the observed heterozygosity (H_O ) ranged from 0.233 to 0.900 and the expected heterozygosity (H_E ) ranged from 0.326 to 0.893, with an average of 7.8 alleles/locus, the polymorphism information content (PIC) ranged from 0.283 to 0.866 (mean 0.731). In CP, the H O ranged from 0.189 to 0.892 and the H E ranged from 0.333 to 0.800, with an average of 4.4 alleles/locus. The probability test showed significant departures from HWE in 9 and 2 of the 21 loci in WP and in CP, respectively. Deficiency of heterozygotes at four loci showed the presence of null alleles (P <0.01). The PIC of 20 microsatellite loci in WP were greater than 0.50. Overall, these novel markers are potentially useful for the study of population genetics, construction of genetic linkage and quantitative trait loci maps in large yellow croaker.  相似文献   

导入bar基因获得裙带菜草丁膦抗性配子体     

任宝永  姜鹏  崔玉琳  姜进举  秦松 《海洋科学》2012,36(1):6-9

裙带菜(Undaria pinnatifida)配子体经2.5 L气升式光生物反应器快速营养增殖,通过基因枪法将携带草丁膦抗性基因(bar)的载体转入扩增后的配子体,经草丁膦筛选,获得抗性克隆。提取抗性克隆配子体基因组总DNA,经PCR和PCR Southern检测,结果显示60%的抗性克隆可检出bar基因,表明bar基因是裙带菜配子体基因工程有效的筛选标记。  相似文献   

基于线粒体D-loop 基因探讨花鳗鲡的群体遗传多样性及其种群进化历史     

丁旭  齐鑫  尹绍武 《海洋科学》2012,36(5):117-123

通过测定花鳗鲡(Anguillia marmorata)海南群体(HN)和菲律宾群体(PH)共19尾个体的线粒体D-loop基因的核苷酸序列(约1017 bp),分析了花鳗鲡的种群遗传结构。结果表明:A、T、G、C 4种核苷酸的平均含量分别为39.8%、28.3%、12.4%、19.4%,A+T含量(68.1%)明显高于G+C含量(31.8%)。所测序列中存在73个变异位点,共有18个单倍型。其中海南群体的单倍型多样度(Hd)、核苷酸多态性(Pi)、平均核苷酸差异数(k)分别为0.982、0.21577和219.655,而菲律宾群体的单倍型多样度、核苷酸多态性、平均核苷酸差异数分别为1.000、0.26728和271.821,两个群体之间平均遗传距离(P)为0.3203。结果表明2个群体遗传变异较大,菲律宾群体的遗传多样性较海南群体更加丰富。通过构建NJ分子系统树表明2个群体的亲缘关系较近。利用中性检验Tajima’s D(海南群体D=1.35345,P>0.01;菲律宾群体D=0.79220,P>0.01)和Fs(海南群体Fs=3.759;菲律宾群体Fs=2.231)探讨其种群历史,表明花鳗鲡群体进化过程种群数量较为稳定。  相似文献   

中国民歌文化景观基因空间分布特征及形成机理     

王芳  周年兴  关健  彭鹏 《热带地理》2015,35(6):797-803

民歌作为中国传统民间文化的艺术代表之一,是在特定地域空间上的景观文化表达。文章根据景观基因理论从本体形态和文化环境2个维度,识别民歌文化景观的基因结构,并结合定量数据分析民歌文化景观基因的空间分布特征。结果表明：就体裁而言,号子主要分布于平原区域,山歌分布于山地,而小调则分布于东部地区长江流域;就题材而言,劳动生产类民歌分布于精耕细作的东部地区,爱情婚姻类民歌分布于远离中原文化的地区;音韵特点而言,徵调式民歌所占比重最大,羽调次之,而角调最少。少数民族的民歌主要分布于西北西南和东北等少数民族人口较多的地区。表演形式也因地域差别具有较大差异。民歌文化景观的形成是人与地理环境相互作用的结果,不同地理环境的居民形成不同的感知空间,从而创造出具有地域文化符号特征的地方民歌。  相似文献   

急性高温低氧胁迫下刺参体腔液细胞miRNA表达谱的构建和靶标基因的功能分析     

尹英超  刘富祥  张玉钦  陈慕雁 《中国海洋大学学报(自然科学版)》2022,(4):30-42

为了探究刺参应对极端高温、低氧环境的内在分子调控机制,促进刺参养殖产业的可持续发展.本研究以刺参体腔液细胞作为研究对象,分别构建了高温、低氧下miRNA的差异表达谱并分析了差异表达miRNAs的靶基因功能.研究表明:高温组中共发掘了20个差异表达miRNA,其中11个上调,9个下调;低氧组中共发掘了10个差异表达miR...  相似文献   

Molecular Cloning and Expression ofPoIR2, a Novel Gene Involved in Immune Response in Japanese Flounder （Paralichthys olivaceus）     

Shuo Li  Chunmei Li  Xubo Wang  Yanan Wang  Zhipeng Liu  Teng Zhai  Quanqi Zhang 《中国海洋大学学报(英文版)》2010,9(1):59-64

A novel immune-related gene was expressed in Japanese flounder (Paralichthys olivaceus) injected with Vibrio anguillarum. The complete cDNA contained a 169 bp 5’UTR, a 336 bp open reading frame (ORF) encoding 111 amino acids and a 556bp 3’UTR. Six exons and five introns were identified in the PoIR2 gene. Blastp similarity comparison showed its encoding protein had 50% similarity to Danio rerio neuromedin S (NMS), but further alignment indicated they did not have NMS C-terminal conservational signature domain. So it was not defined as an NMS homologue. Protein structure analysis indicated it had a 26aa signal peptide and was a secretory pathway protein. RT-PCR demonstrated that the expression of PoIR2 was quickly induced and drastically increased in liver, kidney, spleen, gills, intestine, heart, and skeletal muscle after infected with V. anguillarum. These results indicated that the PoIR2 might play some important role in Japanese flounder immune response system. This gene was named PoIR2 (P.olivaceus immune-related gene 2, GenBank accession number: EU224372). The mature PoIR2 peptide was expressed in BL21(DE3) pLysS using pET-32a(+) vector and a great part of the recombinant mature peptide existed as soluble type.  相似文献   

盐碱地有望变身良田沃土     

《地理教学》2010,(14):62-62

盐碱地基本上都是不毛之地,这是小学课本里就有的常识。但华东师范大学夏涛教授的课题组,最近通过基因改组技术创造出一种新的基因,凡是转入并表达这种新基因的植物,都能够在高盐环境里正常生长。该项成果目前已经申请了中国国家发明专利和国际PCT专利。  相似文献   

传统聚落景观基因信息单元表达机制   总被引：2，自引：0，他引：2  

胡最  刘沛林  申秀英  邓运员  李伯华 《地理与地理信息科学》2010,26(6)

传统聚落景观基因信息单元的表达机制主要包含了逻辑表达机制、数据模型表达机制和自动提取机制.结合聚落景观基因的相关理论和构建景观基因信息图谱的研究实践,探讨了传统聚落景观基因信息单元的表达机制,并以湘西凤凰古城和江永女书为例,进行了表达机制的实验.研究结果表明,表达机制对于构建景观基因图谱不仅具有重要科学意义,而且能极大地提高传统聚落景观信息管理的水平.  相似文献   

Sequences and Phylogeny Analysis of rbcL Gene in Marine Chlorophyta     

SHEN Songdong  LI Yanyan  WU Xunjian  DING Lanping 《青岛海洋大学学报(英文版)》2010,(2):145-150

  相似文献   

Sequence Comparison of Partial Cytochrome b Genes of Two Coilia species   总被引：2，自引：0，他引：2  

LIU Jinxian)  )  GAO Tianxiang)  WANG Yujiang)  and ZHANG Yaping) ) The Key Laboratory of Mariculture  Ministry of Education  Ocean University of China  Qingdao  P.R.China ) Laboratory of Molecular Evolution  Genome Diversity  Kunming Institute of Zoology  Chinese Academy of Sciences  Kunming  P. R. China 《中国海洋大学学报(英文版)》2005,4(1):85-88

1Introduction Japanese grenadier anchovy,Coilia ectenes Jordanet Seale(1905),is distributed in the northwest Pacif-ic.The congener,Osbeck's grenadier anchovy,Coil-ia mystus(Linnaeus,1758),is widely distributed inthe west Pacific.Both species inhabit coastal watersand estuaries and can reach up to rivers.The twospecies are important contributors to the coastal fish-eries of China(Zheng et al.,2003).Many studies on the fishery biology of the twospecies have been conducted(Zheng et al.,2003)…  相似文献