浮游植物叶绿素a含量简易测定方法的比较   总被引：17，自引：0，他引：17       下载免费PDF全文

杨彩根  宋学宏  孙丙耀 《海洋科学》2007,31(1):6-9

用乙醇、DMF(N,N-二甲基甲酰胺)、丙酮3种不同有机溶剂分别萃取苏州环城河水样中的浮游植物细胞叶绿素a,在分光光度计上测定其叶绿素a含量,试验数据经方差分析、Q检验,结果显示,乙醇法、DMF法的叶绿素a萃取率极显著高于丙酮(F=54.20＞F0.01(2,18)(6.01)).另用这3种萃取方法分别测定无常蓝纤维藻(Dactylococcopsis irregularis )纯培养液叶绿素a含量,通过直线回归,同样证明乙醇法、DMF法重复率较高,稳定性好,萃取率高.从安全、简便、重复性高等方面综合考虑,认为用乙醇萃取法是目前水域环境中浮游植物叶绿素a简易测定的最佳方法.  相似文献   

Phytoplankton biomass and size fractions in surface waters of the Australian sector of the southern ocean     

Wendy Ehnert  C. Peter McRoy 《Journal of Oceanography》2007,63(2):179-187

We collected surface water along the 142nd E meridian from Tasmania to Antarctica in December 1999. We measured temperature, salinity and total chlorophyll a; additionally, we collected suspended particle size fractions and used fluorometric analysis to determine the quantity of chlorophyll a in each of four cell size classes: picoplankton (<3 μm), two nanoplankton fractions (3–10 μm and 10–20 μm) and microplankton (> 20 μm). Changes in temperature and salinity show that we crossed 6 water masses separated by 5 fronts. We found low abundance (<0.2 mg m⁻³) of chlorophyll in all size classes, with the exception of higher values near the continent (0.2 to 0.4 mg m⁻³). Lowest chlorophyll values (<0.1 mg m⁻³) were found in the Polar Frontal Zone (51° to 54°S). Microplankton made up the largest portion of total chlorophyll throughout most of the region. We conclude that biomass of all phytoplankton fractions, especially pico-and nanoplankton, was constrained by limiting factors, most probably iron, throughout the region and that ecosystem dynamics within a zone are not circumpolar but are regionalized within sectors.  相似文献   

Statistics of nonlinear wave crests and groups   总被引：1，自引：0，他引：1  

M. Aziz Tayfun   《Ocean Engineering》2006,33(11-12):1589-1622

Groups of large nonlinear waves with sharper higher crests can pose hazards to ships, induce harbor resonance and cause wave-overtopping of fixed and floating structures. Past interest in wave groups has mostly been focused on the statistics and modeling of linear wave groups. Studies on nonlinear wave groups are surprisingly few, and address deep water waves only. Here, statistics of nonlinear wave crests and wave-crest groups in deep and transitional water depths are considered, using an appropriate second-order representation for crest heights and the continuous wave-envelope approach. In particular, theoretical expressions describing the statistics of nonlinear wave crests and their groups are posed in the form of a simple second-order transformation of well-known results on linear waves. Predictions from the transformation so posed compare well with nonlinear wave data gathered in the North Sea, and demonstrate that nonlinearities do affect the statistics of large wave crests and their groups significantly.  相似文献   

Small-scale variability of zooplankton pyruvate kinase activity in the Gironde Estuary plume (Atlantic French Coast): A case study under unusually low freshwater discharge   总被引：1，自引：0，他引：1  

Jean-Pierre Bergeron   《Estuarine, Coastal and Shelf Science》2006,69(3-4):662

Pyruvate kinase (PK) activity measurements are used to assess the role of carbohydrates in global feeding of mesozooplankton communities inhabiting an estuary plume. As a consequence of a remarkably low freshwater discharge rate, the sea surface layers of the area under estuarine influence showed a very moderate salinity fall and a nearly total depletion in nitrates, whereas higher levels of these nutrients were found in deeper, more saline, layers. Small-scale PK activity variations in mesozooplankton appear to be closely correlated to nitrate integration values within the water column. The results were analysed in comparison with literature reports. The study produced a coherent overall interpretation, which strongly supports the reliability of this new biochemical tool in detecting assimilation of trace carbohydrates in the diet of mesozooplankton.  相似文献   

Influence of upwelling and river runoff interaction on phytoplankton assemblages in a Middle Galician Ria and Comparison with northern and southern rias (NW Iberian Peninsula)   总被引：2，自引：2，他引：2  

Manuel Varela  Ricardo Prego  Yolanda Pazos  ngeles Moroo 《Estuarine, Coastal and Shelf Science》2005,64(4):721-737

The first oceanographic research (hydrography, nutrient salts, chlorophyll, primary production and phytoplankton assemblages) in a Middle Galician Ria was carried out in Corme-Laxe during 2001, just a year before the Prestige oil spill, being the only reference to evaluate eventual changes in the phytoplankton community. Due to the small size of this ria (6.5 km²), oceanographic processes were driven by the continental water supplied by Anllons River during the wet season (20–30 m³ s⁻¹ in winter), and the strong oceanic influence from the nearby shelf during the dry season. The annual cycle showed a spring bloom with high levels of chlorophyll (up to 14 μg Chl-a L⁻¹) and primary production (3 g C m⁻² d⁻¹) and a summer upwelling bloom (up to 8 μg Chl-a L⁻¹ and 10 g C m⁻² d⁻¹) where the proximity of the Galician upwelling core (<13.5 °C at sea surface) favors the input of upwelled seawater (up to 9 μM of nitrate and silicate) to the bottom ria layer, even during summer stratification events (primary production around 2 g C m⁻² d⁻¹). Thus, phytoplankton assemblages form a “continuum” from spring to autumn with a predominance of diatoms and overlapping species between consecutive periods; only in autumn dinoflagellates and flagellates characterized the phytoplankton community. In the Middle Rias as Corme-Laxe, the nutrient values, Chl-a, primary production and phytoplankton abundance for productive periods were higher than those reported for the Northern (Ria of A Coruña) and Southern Rias (Ria of Arousa) for year 2001; this suggests the importance of the hydrographic events occurring in the zone of maximum upwelling intensity of the Western Iberian Shelf, where a lack of annual cycles studies exists.  相似文献   

Influences of Nutrient Outwelling from the Mangrove Swamp on the Distribution of Phytoplankton in the Matang Mangrove Estuary, Malaysia   总被引：1，自引：0，他引：1  

Katsuhisa Tanaka  Poh-Sze Choo 《Journal of Oceanography》2000,56(1):69-78

Distributions of dissolved nutrients and Chl. a were investigated in the Sangga Besar River Estuary in the well-managed Matang Mangrove Forest in West Malaysia. In the estuary, spring tide concentrations of ammonium, silicate and phosphate were higher than those in the neap tide, which suggests that these nutrients are flushed from the mangrove area by the inundation and tidal mixing of the spring tide. Ammonium comprised over 50% of the dissolved inorganic nitrogen in the spring tide, while nitrite tended to dominate in the neap tide, indicating the predominance of nitrification inside the estuary in neap tides. Nutrient concentrations in the creek water were higher than those of estuarine water, indicating the nutrient outwelling from the mangrove swamp and ammonium regeneration from mangrove litter in the creek sediments. The maximum concentration of Chl. a in spring tides reached 80 g/l while it was below 20 g/l in the neap tides. These variations in the phytoplankton biomass and nutrients probably reflect the greater nutrient availability in the spring tide due to outwelling from the mangrove swamp and creek.  相似文献   

氮磷营养盐与有机汞联合作用对微氏海链藻生长的影响   总被引：1，自引：1，他引：1  

李顺兴  郑凤英  洪华生  王大志  黄立漳 《海洋科学》2005,29(10):36-39

探讨在高营养盐（氮、磷、硅分别为浮游植物生长的营养盐阚值≥40、50、9倍）条件下，氮浓度及氮磷摩尔比值[n（N）／n（P）]变化、汞（甲基汞和乙基汞）对微氏海链藻（Thalassiosira weissflogii）生长的影响。结果表明，高营养盐区海链藻的生长遵循营养盐限制的唯一因子论；氮浓度和氮磷比提高促进生长，当n（N）／n（P）=64则呈抑制效应；生长初期，甲基汞和乙基汞均产生毒性抑制作用，生长中后期，适量甲基汞（浓度≤1．0μg／L）、乙基汞（浓度≤0．5μg／L）却呈兴奋效应，促进生长；汞形态不同，剂量-效应关系不同。氮和有机汞是海链藻生长的刺激因子。适度有机汞污染与氮富营养化对微氏海链藻的生长产生协同效应。  相似文献   

Size-Fractionated Primary Production Estimated by a Two-Phytoplankton Community Model Applicable to Ocean Color Remote Sensing   总被引：3，自引：0，他引：3  

Takahiko?KamedaEmail author  Joji?Ishizaka 《Journal of Oceanography》2005,61(4):663-672

In order to estimate primary production from ocean color satellite data using the Vertical Generalized Production Model (VGPM; Behrenfeld and Falkowski, 1997), we propose a two-phytoplankton community model. This model is based on the two assumptions that changes in chlorophyll concentration result from changes of large-sized phytoplankton abundance, and chlorophyll specific productivity of phytoplankton tends to be inversely proportional to phytoplankton size. Based on the analysis of primary production data, P _opt ^B , which was one parameter in the VGPM, was modeled as a function of sea surface temperature and sea surface chlorophyll concentration. The two-phytoplankton community model incorporated into the VGPM gave good estimates in a relatively high productive area. Size-fractionated primary production was estimated by the two-phytoplankton community model, and P _opt ^B of small-sized phytoplankton was 4.5 times that of large-sized phytoplankton. This result fell into the ranges observed during field studies.  相似文献   

Observations on the surface water characteristics in the western Irish Sea: July 1977     

J. Beardall  P. Foster  D. Voltolina  G. Savidge 《Estuarine, Coastal and Shelf Science》1982,14(6):589-598

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细菌、病毒与浮游植物相互关系及其对海洋地球化学循环的作用   总被引：9，自引：0，他引：9  

周玉航  潘建明  叶瑛  周怀阳 《台湾海峡》2001,20(3):340-345

本文综述了近年来国内外在海洋地球化学循环，特别是微生物环研究方面的发展现状，探讨了浮游植物，细菌，病毒相互之间的关系及其对海洋地球化学循环的影响。  相似文献