Low genetic variation in perch (Perca fluviatilis L.) from three major European drainage systems in Switzerland     

Hansjakob Heldstab  Masaya Katoh 《Aquatic Sciences - Research Across Boundaries》1995,57(1):14-19

Previous genetic studies on perch (Perca fluviatilis L.) in northern and southern regions of Europe have shown low heterozygosity. No such investigation has been conducted in the central part of Europe. The genetic variability ofP. fluviatilis in four Swiss lakes (Lake Constance, Lake Zürich, Lake Geneva and Lake Maggiore) was investigated. These four lakes belong to three different drainage systems (Rhine, Rhone and Po) without connections. A total of 136 fish were analysed electrophoretically. Only one locus (SOD ^*) was highly polymorphic in Lake Constance among the 27 loci studied. The expected heterozygosities for each population and for the total population were higher than in the previous studies but low (H _S= 0.38–2.7%;H _T=1.22%) and Nei's genetic distances were small (0.000–0.003). However, the jack-knifed mean of the Wright'sF _STwas calculated as 0.142, which indicates modest genetic differentiation among the perch populations in Switzerland, due to the differentiated population in Lake Constance.  相似文献   

溶胶－凝胶方法制取纳米级超导物质的研究     

高京林 《现代地质》1996,(3)

当前对超导物质的研究都集中于高临界温度的超薄型物质的研究上，本研究通过相对简单的方法获得高纯度的纳米级的钙钛矿型超导物质，其过程为：通过溶胶－凝胶方法合成一种含有Ｌａ和Ｎｉ的高纯度的有机物，然后利用天然蒙脱石的离子交换性将上述化合物渗入到其含水层中以获得超薄型的超导物质  相似文献   

Analytical problems in determining peptide nitrogen in the humic fractions of three Italian soils     

A. Ramunni  R. Scialdone  D. Felleca 《Organic Geochemistry》1985,8(1):87-93

Peptides were released from organic matter fractions of three Italian soils (humin, humic and fulvic acids), when the samples were hydrolyzed in Ba(ON)₂-saturated solution at 105°C for 2 hr. The peptides obtained were separated using electrophoresis and paper chromatography. The presence of polypeptides in the soil organic matter was indicated by: (1) their hydrolysis by pronase; (2) the amino acids released by 6 N HCl hydrolysis; (3) The comparison of i.r. spectra of humic fractions before and after hydrolysis with 6 N HCl.Attempts at isolating the native proteinaceous compounds using electrophoresis in polyacrylamide gel failed; additionally, our attempts to hydrolyze proteinaceous components enzymatically in unfractionated soil organic matter, as well as in its fractions, before and after methylation, with pepsin, papain and pronase, were unsuccessful. Pronase demonstrated a weak proteolytic activity only at very low substrate-enzyme ratios (20 : 1) in humic and fulvic fractions and in whole phyrophosphate extract. Deproteinated substrates treated with pronase also released free amino acids, suggesting autodigestion.In humin, humic and fulvic fractions we found a total amino acid content of 40–45%, 12–24% or 1–85, respectively. Amino acid recovery from single fractions was about 70–80% of the total content in the unfractionated soil.  相似文献   

Vertical distribution of the microbial community in modern lacustrine sediments of Taihu Lake.     

DIAO Xin-Jing YANG Liu-Yan YIN Da-Jiang WANG Ru-Cheng LIU Xian-Cai LIU Jian-Jun 《地学前缘》2008,15(6):177

  相似文献   

硅胶／壳聚糖吸附材料的制备及表征     

张玉玉  王以明  蔡国军 《地质灾害与环境保护》2008,19(3):109-112

利用γ-氨基丙基-三乙氧基硅烷偶联剂与活化硅胶表面的羟基反应,制备了硅烷化硅胶,所得产物与壳聚糖反应合成了硅胶／壳聚糖复合吸附材料。通过红外光谱分析（IR）,扫描电镜（SEM）及能谱分析对复合材料进行表征,并测试了硅胶／壳聚糖复合材料对Zn^2＋、Cd^2＋的吸附能力。结果表明：复合吸附材料对Zn^2＋、Cd^2＋具有很好的吸附能力,最大吸附率为96．01％及90．65％。  相似文献   

Isozymes Analysis of the Golden Cuttlefish Sepia esculenta (Cephalopoda: Sepiidae)   总被引：3，自引：0，他引：3  

ZHENG Xiaodong )  *  ZHAO Jianmin )  XIAO Shu )  WANG Rucai )  WANG Shidang )  ZHOU Weiwu ) ) The Key Laboratory of Mariculture  Ministry of Education  Ocean University of China  Qingdao  P..R.China ) Wendeng Fishery Technical Extension Station  Weihai  P..R.China 《中国海洋大学学报(英文版)》2004,3(1)

1　Introduction　ThegoldencuttlefishSepiaesculenta,aneriticanddemersalspecies,livesinthedepthof 1 0 - 1 0 0meters,andsometimesinseabottomsand .Itiswidelydistrib utedfromcentralHonshutoVietnamandthePhilip pines .Thegoldencuttlefishisalsoanimportantobjectof…  相似文献   

恩平24-2油田水平井钻井液技术研究     

胡友林  岳前升  刘书杰  朱磊 《海洋地质译丛》2014,(2):93-96

为满足恩平24-2油田水平井钻井工程和储层保护需要,通过实验研制出一种无固相快速弱凝胶钻井液体系.室内评价试验表明：该钻井液具有较高的动塑比和较高低剪切速率黏度（LSRV）,能有效克服水平井段携岩难、易形成岩屑床的问题.具有优异的抑制性和润滑性,且滤失量较小,具有较强的抗温、抗污染能力、井眼净化能力和储层保护能力.针对恩平24-2油田水平井采用裸眼完井工艺,研究了破胶剂JPC对钻井液及其滤饼的破胶性能,破胶剂JPC能有效解除聚合物对储层造成的污染.研究结果表明,该钻井液具有独特的流变性,维护简单,优异的储层保护性能和较好的可降解性,适用于恩平24-2油田水平井水平段的钻进.  相似文献   

两种鲨鱼鳃部真菌菌群的PCR-DGGE分析     

张翼  穆军  冯妍  阎松 《海洋与湖沼》2014,45(1):183-188

以提取自东海海域两种鲨鱼(尖吻鲭鲨和噬人鲨)鳃耙组织的总DNA为模板,对其真菌菌群rDNA的基因内转录间隔区ITS1序列进行了PCR扩增,扩增产物经变性梯度凝胶电泳(DGGE)分离,电泳图谱中的7条主要条带经过二次PCR扩增后进行了克隆测序。NCBI-BLAST比对及分子系统发育分析结果表明,尖吻鲭鲨鳃部真菌菌群的优势种来自四个分类单元,其中一个来自青霉属,三个来自曲霉属;噬人鲨鳃部真菌的优势种来自四个分类单元,其中一种来自青霉属、两个来自梗孢酵母属、一个来自枝顶孢属;青霉属为两种鲨鱼鳃部共同的优势菌;另外DGGE指纹图谱显示还有种类较丰富的劣势菌存在。数据库检索显示多数真菌分类单元具有较好的产生生物活性天然产物的潜力。该PCR-DGGE分析揭示鲨鱼鳃部栖生有较丰富的真菌类群,它们的代谢产物及其与宿主之间的相互作用还有待深入研究。  相似文献   

弱凝胶体系地层流体转向技术在海上稠油油田的应用     

马奎前  蔡晖  吴小张  张宏友  何芬 《海洋地质译丛》2011,(2):65-67,74

稠油油田注水开发过程中,受不利流度比和非均质性的影响,注入水沿高渗层突进,造成油井暴性水淹,严重影响水驱开发效果。针对海上稠油油田的开发特点和存在问题,对弱凝胶体系的适用性进行了分析,对影响凝胶体系调驱的关键参数进行了优化。结果表明,原油黏度影响弱凝胶调驱效果,随着黏度的增大调驱效果有变差趋势。在含水率40％以后,注入时机越晚,调驱效果越差。注入量越大,调驱效果越好,在实际生产过程中考虑注入能力和经济因素应采取合理的注入量。先导性弱凝胶试验的成功,为海上稠油油田的稳油控水工作指明了方向。  相似文献   

Purification and characterization of acetylcholinesterase from brain tissues of Oreochromis aurea and its application in environmental pesticide monitoring     

YunHua Ding  XiaoMin Wu  JunBin Fang 《寒旱区科学》2011,3(4):0339-0343

Acetylcholinesterase (AChE) plays an important role in enzyme-based detection of pesticides in the environment. In this paper, AChE from the Triton X-100 extract of brain tissues of Oreochromis aurea was purified by (NH₄)₂SO₄ fractional precipitation, Sephadex G-100 gel filtration, and DEAE-cellulose ion exchange chromatography. Certain biochemical characterizations of the purified enzyme and inhibition of pesticides on the enzyme were also studied. The specific activity of this purified enzyme was 20.628 U/mg protein, fold of purification was 139, and recovery was 22.1%. The optimal temperature of this enzyme was between 35–40 °C, and optimal pH was between 7.5–8.0. The Michaelis constant (K_m) for acetylthiocholine iodide was 0.183 mmol/L. The enzyme activity was inhibited by excess substrate, and optimal substrate concentration was 6 mmol/L. Four pesticides (dichlorvos, phoxim, triazophos, and methomyl) exhibited strong inhibitions on this enzyme with IC₅₀ less than 5 μg/mL. This study suggests that Oreochromis aurea (tilapia) could be a good enzyme source for pesticide monitoring in water environments.  相似文献