Development of a real-time PCR method for Thalassiosira rotula rapid detection     

HE Shanying  YU Zhigang  MI Tiezhu 《海洋学报(英文版)》2007,26(2):133-139

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.  相似文献   

潮滩演变规律在围堤选线中的应用——以江苏辐射沙洲内缘区为例   总被引：2，自引：0，他引：2  

李加林  王艳红  张忍顺  张正龙  齐德利  顾勇 《海洋工程》2006,24(2):100-106

滩涂围垦是沿海地区耕地占补平衡的重要手段。如何进行合理的围堤选线,尽可能减小围堤对潮滩水沙环境的影响,使匡围后的潮滩演变不影响海堤安全,是围海工程设计时必须考虑的问题。以江苏辐射沙洲内缘区仓东垦区匡围工程为例,运用潮滩均衡态概念框架,对条子泥西侧岸滩平均高潮位点变化、师地岛形成及潮沟活动性等潮滩演变特征及趋势进行了分析,并重点探讨了其在围堤选线中的应用。  相似文献   

日本凋毛藻碳酸酐酶基因及其编码的蛋白特征     

刘晨临  黄晓航  LEE Yookyung  LEE Hungkum  李光友 《海洋学报》2006,28(3):161-166

碳酸酐酶CA(EC 4.2.1.1)是含锌原子的金属蛋白酶,该酶的基本功能是催化CO₂与HCO₃-间的相互转换[1].该反应在许多生物过程中都发挥着重要的作用,例如离子交换、呼吸作用、pH稳定性、捕获CO₂和光合作用等[2~5].CA广泛分布于各种生物中,已知的CA酶根据氨基酸序列被分为-αCA,-βCA和γ-CA三种类型[5~7].这3种类型的CA酶序列间的相似性很低,被认为是由不同的途径进化而来的[7].α-型的CA酶在动物、植物、绿藻、细菌和蓝细菌中都有发现[7],在藻类中报道的α-型CA还很少.迄今为止只在衣藻中发现2种异构体,在盐藻中发现3种[8,9],在红藻中还未见报道.  相似文献   

Effects of winds,tides and storm surges on ocean surface waves in the Sea of Japan     

ZHAO Wei  TIAN Jiwei  LI Peiliang  HOU Yijun 《海洋学报(英文版)》2007,26(3):9-21

Ocean surface waves are strongly forced by high wind conditions associated with winter storms in the Sea of Japan. They are also modulated by tides and storm surges. The effects of the variability in surface wind forcing, tides and storm surges on the waves are investigated using a wave model, a high-resolution atmospheric mesoscale model and a hydrodynamic ocean circulation model. Five month-long wave model simulations are inducted to examine the sensitivity of ocean waves to various wind forcing fields, tides and storm surges during January 1997. Compared with observed mean wave parameters, results indicate that the high frequency variability in the surface wind filed has very great effect on wave simulation. Tides and storm surges have a significant impact on the waves in nearshores of the Tsushima-kaihyō, but not for other regions in the Sea of Japan. High spatial and temporal resolution and good quality surface wind products will be crucial for the prediction of surface waves in the JES and other marginal seas, especially near the coastal regions.  相似文献   

潮汐性质相似性判断的两个指标及其应用   总被引：1，自引：0，他引：1  

黄辰虎  马福诚  吕良  许家琨  孙雪杰 《海洋测绘》2007,27(6):12-15

提出采用时差法的相关系数和最小二乘潮位拟合法的推算中误差,作为潮汐性质相似性强弱判断的两个量化指标。根据实测潮汐数据进行了有关计算,结果表明这两个指标可以用于潮汐性质相似性强弱的快速判断,另外推算中误差还可以用于验潮站潮位数据缺测时推算精度的检验,建议在沿岸海道测量水位改正作业中综合使用。  相似文献   

The impact of the M₂ internal tide on data-assimilative model estimates of the surface tide     

E.D. Zaron  G.D. Egbert   《Ocean Modelling》2007,18(3-4):210-216

We use a synthetic data experiment to assess the accuracy of ocean tides estimated from satellite altimetry data, with emphasis on the impact of the phase-locked internal tide, which has a surface expression of several centimeters near its sites of genesis. Previous tidal estimates have regarded this signal as a random measurement error; however, it is deterministic and not scale-separated from the barotropic (surface) tide around complex bathymetric features. The synthetic data experiments show that the internal tide has a negligible impact on the barotropic tidal fields inferred under these circumstances, and the barotropic dissipation (a quadratic functional of the tidal fields) is in good agreement with the energetics of the three-dimensional regional primitive equations model which is the source of the synthetic data.  相似文献   

真鲷早期发育阶段行为生态学研究     

刘晓春  何大仁  李大勇 《热带海洋学报》1993,(3)

真鲷Pagrosomus major Temminck et Schlegel早期发育阶段游泳行为的发育可以分为:(1)旋转式运动;(2)阶梯型运动;(3)间断式水平运动和滑翔;(4)巡游以及(5)疾游五种类型。前期仔鱼的摄食方式主要是伴有甩头动作的前冲摄食;后期仔鱼的摄食主要有两种类型:一种是身体弯成S形的“S攻击型”;另一种为不作摄食姿势的“咬食攻击型”。摄食方式随着发育水平和饵料种类的不同而异。文中对发育过程中出现的攻击行为、护食行为、互食行为、猝死现象亦进行了描述。并对游泳、摄食以及各种行为的生态意义和实际意义进行了讨论。  相似文献   

Numerical study of enhanced energy dissipation near a seamount     

Nobuyuki Iwamae  Toshiyuki Hibiya  Yoshihiro Niwa 《Journal of Oceanography》2006,62(6):851-858

Using a two-dimensional primitive equation model, we examine nonlinear responses of a semidiurnal tidal flow impinging on a seamount with a background Garrett-Munk-like (GM-like) internal wavefield. It is found that horizontally elongated pancake-like structures of high vertical wavenumber near-inertial current shear are created both in the near-field (the region over the slope of the seamount) and far-field (the region over the flat bottom of the ocean). An important distinction is that the high vertical wavenumber near-inertial current shear is amplified only at mid-latitudes in the far-field (owing to a parametric subharmonic instability (PSI)), whereas it is amplified both at mid-and high-latitudes (above the latitude where PSI can occur) in the near-field. In order to clarify the generating mechanism for the strong shear in the near-field, additional numerical experiments are carried out with the GM-like background internal waves removed. The experiments show that the strong shear is also created, indicating that it is not caused by the interaction between the background GM-like internal waves and the semidiurnal internal tides. One possible explanation is proposed for the amplification of high vertical wavenumber near-inertial current shear in the near-field where tide residual flow resulting from tide-topography interaction plays an important role in transferring energy from high-mode internal tides to near-inertial internal waves.  相似文献   

潮汐调和分析的一种算法   总被引：1，自引：1，他引：1  

宋志尧  严以新  茅丽华 《海洋工程》1997,(3)

本文在常规潮汐调和分析方法的基础上，提出了一种由高低潮资料进行潮汐分析的算法。具体计算表明，该算法与现今通用的常规算法（即等间隔最小二乘法）相比，既可大大减少所需原始样本量，在相同记录长度内，是常规算法的三分之一还少，同时减少样本处理的前期工作量；又能保证潮位拟合的精度和预报的可信度，两者精度相当，结果一致。该算法原理简单、实用有效，对于局部样本缺损较易处理，具有实际应用价值。  相似文献   

派比安台风对上海黄浦江潮位的影响及成因探讨   总被引：3，自引：3，他引：3  

徐建成 《海洋预报》2001,18(1):1-10

2000年派比安台风产生的历史第二高潮位对上海的防汛带来严重影响。本文通过比较历史上的风暴潮，从动力机制、天文高潮与增水极值相碰头，潮波共振和水利工程的影响等四个方面分析了这次风暴潮造成的高水位，及台风余振期边缘波的影响，并用SLOSH（Sea,Lake,Overland Surge from Hurricanes)模式模拟了这次风暴潮，为防台减灾的正确决策提供了理论依据。  相似文献