Influence of environmental disturbances and reproductive strategy on genetic diversity and differentiation of Zostera marina populations on the southern coast of Korea     

Young Kyun Kim  Seung Hyeon Kim  Joo Mi Yi  Sang Rul Park  Kun‐Seop Lee 《Marine Ecology》2019,40(1)

The genetic diversity and differentiation of four Zostera marina populations along the southern coast of Korea were estimated using random amplified polymorphic DNA (RAPD) markers to determine the effects of natural and anthropogenic stresses and reproductive strategy on within‐population genetic diversity. The mean number of alleles and gene diversities, indicating population genetic diversity, was highest in the Z. marina population that was exposed to repeated environmental disturbances, and lowest in the most undisturbed population. The higher genetic diversity in the disturbed population was associated with a higher contribution of sexual reproduction to population persistence. This suggests that both the level of disturbances and the reproductive strategy for population persistence contributed significantly to population genetic diversity at the study sites. According to the analysis of molecular variance (AMOVA), 76% genetic variation was attributable to differences among individuals within populations. The observed genetic differentiation (F_ST = 0.241) among Z. marina populations at the study sites appeared to result from reduced meadow size, increased genetic drift, and a high incidence of asexual reproduction. Increased population genetic diversity can enhance resistance and resilience to environmental disturbances; thus, this investigation of seagrass population genetics provides valuable new insights for the conservation, management, and restoration of seagrass habitats.  相似文献   

南海鸢乌贼中型群雄性个体肌肉和性腺组织能量积累及其分配     

朱凯  张立川  陈新军  姚吉祥  韩飞  林东明 《热带海洋学报》2019,38(4):41-51

利用组织能量密度测定技术和残差指标分析方法, 分析了南海鸢乌贼中型群雄性个体肌肉组织和性腺组织的能量积累及其分配变化过程。结果显示, 胴体、尾鳍、足腕、精荚复合体、精巢等组织的能量密度值分别为21.16±1.44、21.98±1.51、21.44±1.50、20.83±1.70和21.41±1.70kJ?g ^-1。胴体和精荚复合体两组织的能量密度在不同性腺成熟度之间均存在显著性差异, 而尾鳍、足腕、精巢等3种组织的能量密度则在不同性腺成熟度之间均没有统计学差异。个体总能量积累为299.31±90.81kJ; 随着个体生长, 总能量积累呈现增加的趋势。胴体、足腕和尾鳍等肌肉组织的能量占比为95.31%~98.04%, 随着性腺发育呈现降低的趋势; 精巢和精荚复合体等性腺组织的能量总投入占比为2.63%~5.33%, 随着性腺发育呈增加的趋势, 并在Ⅴ期达到最大值。同时, 残差指标分析显示, 胴体、尾鳍、足腕等肌肉组织能量积累-胴长残差与性腺组织能量积累-胴长残差呈显著的正相关关系, 表明个体性腺发育过程中肌肉组织能量没有转化用于性腺组织的生长发育, 其生殖能量投入倾向于外源性投入。初步掌握了南海鸢乌贼中型群雄性个体肌肉和性腺组织能量的积累变化过程, 为深入了解它们的繁殖策略和为可持续开发利用该种类资源提供了科学基础。  相似文献   

RNA干扰蚤状溞(Daphnia pulex)doublesex1基因的表达研究     

童巧琼  林重远  朱晓静  周伟  徐善良  王丹丽  赵云龙 《海洋与湖沼》2018,49(1):117-123

为了探索doublesex1(dsx1)基因是否在蚤状溞的生殖转换过程中发挥作用,利用蚤状溞滤食摄食的特点将体外合成的dsx1双链RNA(ds RNA)通过浸泡得方法分别摄入不同生殖状态的溞体中,实现dsx1基因的表达沉默。随后采用荧光定量PCR(q PCR)以及整体原位杂交分别检测不同生殖状态下蚤状溞在RNAi前后体内dsx1的表达水平变化,进而研究dsx1在蚤状溞生殖转换过程中的作用。结果表明:雄性溞、两性溞、孤雌溞在RNAi后均出现了dsx1 m RNA表达水平的显著下调,且下调量具有显著性差异(P0.05)其中在孤雌溞中下调68%,在三种生殖状态中最为显著,其次是雄性溞(56%)和两性溞(20%)。同时发现,干扰前能够在溞的第一触角、第一胸肢和复眼上检测到明显的信号位点,而在RNAi后,仅在溞体的第一触角上发现少量dsx1基因表达位点,且第一胸肢及复眼上未检测到相关信号。结合未干扰前dsx1在不同生殖状态溞的表达定量以及定位结果,我们推测dsx1可能在蚤状溞的生殖转化基因调控过程中扮演关键角色,并且在维持雄性蚤状溞性别特征的机制中发挥重要作用。  相似文献   

荣成俚岛野生鼠尾藻资源调查     

王翔宇  詹冬梅  刘洪军 《海洋湖沼通报》2018,(1):81-84

2014年4月至2015年2月对荣成俚岛湾鼠尾藻(Sargassum thunbergii)的生长情况进行了调查。实验设计采用样方法观测了鼠尾藻体长结构的季节变化和繁殖特性。结果表明,(1)鼠尾藻一年有一次繁殖季节,有性繁殖盛期在6月下旬至7月下旬;(2)不同潮带间的主枝平均长度由大到小依次为低潮带、中潮带和高潮带,并且低潮带的SGR最大值出现的时间较中潮带和高潮带早;(3)在6月和7月体长结构组成最丰富。  相似文献   

人工养殖条件下多齿围沙蚕亲体培育和繁殖生物学的初步研究   总被引：2，自引：0，他引：2  

杜荣斌  刘红梅  郑家声  冯俊荣  刘立明  王勇强 《海洋科学》2009,33(11):37-43

通过生态学及组织学方法对人工养殖条件下多齿围沙蚕(Perinereis nuntia Savigny)亲体培育条件及繁殖生物学进行了研究.结果表明,多齿围沙蚕雌雄异体,非成熟季节外观上无法辨别雌雄,性成熟时,雌(异沙蚕体)灰绿色,雄(异沙蚕体)红白色.生殖细胞起源于体腔膜上皮,不久就释放到体腔中,在体腔内卵原细胞和精原细胞http://ebook/成都,今夜请将我遗忘/index.htm分裂增殖形成卵原细胞群和精原细胞群,体腔中充满了外形不规则或近圆形的滋养细胞,细胞内可见嗜伊红颗粒,滋养细胞之间可见发达的微血管.卵原细胞群不断增殖发育,至初级卵母细胞后游离至体腔中继续发育为次级卵母细胞和成熟卵子;精原细胞群形成精子合胞体(syncytium),各级精母细胞在合胞体内发育,直至形成成熟精子时才分散游离.水温18℃以上,多齿围沙蚕的生长体经培育才可能形成异沙蚕体.特别是在经历了一定的低温期后,再提高温度可以有效的促进异沙蚕体形成.胚胎发育的适宜温度为18～25℃;适宜盐度为25～32.胚胎发育过程经历卵裂期、囊胚期、原肠期、担轮幼虫期、后担轮幼虫期、膜内游毛幼虫期等阶段,在水温25℃、盐度30条件下,从受精至孵出3刚节幼虫约需65 h.3刚节游毛幼虫至10刚节幼体约需28 d.  相似文献   

Causes and consequences of life-history variation in North American stocks of Pacific cod     

Ormseth  Olav A.; Norcross  Brenda L. 《ICES Journal of Marine Science》2009,66(2):349-357

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纹藤壶和鳞笠藤壶雄性生殖系统的初步研究     

王艺磊  张子平  成华  李少菁 《热带海洋学报》1999,(4)

对厦门海区的纹藤壶　Balanusamphitriteamphitrite和鳞笠藤壶　Tetraclitasquamosasquamosa　雄性生殖系统进行解剖学、组织学和细胞化学的研究。结果表明:两种藤壶的雄性生殖系统均由精巢、输精管、贮精囊及交接器4部分组成。精巢内依次排列有精原细胞、精母细胞、精子细胞和成熟精子。输精管由一层上皮细胞围成,内可见成熟精子,贮精囊前端管壁结构与输精管相似,后端管壁厚,可分为4层结构。交接器结构复杂,内层为射精管,外层结构与贮精囊后段相似。精子为线形,头部附属小滴内有4—6个致密斑,鞭毛沿头部一侧着生。初步探讨了不同发育阶段的雄性生殖细胞碱性蛋白的变化及附属小滴的细胞化学特性及生理学特性  相似文献   

Population Dynamics and Reproductive Traits of Phytal Gastropods in Seagrass Bed in Otsuchi Bay, North-Eastern Japan   总被引：1，自引：0，他引：1  

Tetsuhiko Toyohara  Masahiro Nakaoka  & Keiko Aioi 《Marine Ecology》1999,20(3-4):273-289

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Changes of melatonin and its receptors in synchronizing turbot (Scophthalmus maximus) seasonal reproduction and maturation rhythm     

Chunyan Zhao  Shihong Xu  Yifan Liu  Chengcheng Feng  Yongshuang Xiao  Yanfeng Wang  Qinghua Liu  Jun Li 《海洋学报(英文版)》2022,41(1):84-98

In most fish, reproduction is seasonal or periodic under the suitable conditions. In turbot(Scophthalmus maximus) farms, one of the most economically important marine flatfish species, changes in daylength could cause changes in the spawning time. In this study, to characterize the regulation of reproductive physiology following light signals, three melatonin receptors(Mtnr) investigated in turbot were named sm Mtnr1, sm Mtnr2,and sm Mtnr1 c. Distinct expression profiles demonstrated that Mtnr m...  相似文献   

The oldest angiosperm—a tricarpous female reproductive fossil from western Liaoning Province, NE China   总被引：2，自引：0，他引：2  

Shuying Duan 《中国科学D辑(英文版)》1998,41(1):14-20

The fossil plant female reproductive organs each with a tricarpous were collected from the Yixian Formation, Chaoyang district, western Liaoning Province. The geological age of the Yixian Formation here is believed to be Late Jurassic, which places this fossil as the oldest angiosperm currently known. Its morphological characters are distinct from other known angiosperm reproductive organs as it has 3 ovaries, each with a terminal style and an apical stigma. 9 such organs compose a cyme. As this morphological arrangement is unique to this plant, a new genus and species has been established, namedChaoyangia liangii gen. et sp. nov. Project supported by the Special Finances of Biological Sciences and Technology, Chinese Academy of Sciences (the special item of the Ministry of Finance of the People’s Republic of China).  相似文献