Variability of heat shock proteins and glutathione S-transferase in gill and digestive gland of blue mussel,Mytilus edulis     

Lyons C  Dowling V  Tedengren M  Gardeström J  Hartl MG  O'Brien N  van Pelt FN  O'Halloran J  Sheehan D 《Marine environmental research》2003,56(5):585-597

Glutathione S-transferase (GST) and heat shock proteins (hsps) 40, 60, 70 and 90 were determined by immunoblotting using actin as an internal control in Mytilus edulis from one station outside (site1) and three stations within (sites 2-4) Cork Harbour, Ireland. Comparisons were made between gill and digestive gland and between sites. Gill shows generally higher hsp 60, 70 and 90 while digestive gland has higher hsp 40. Site 1 showed higher gill hsps 40 and 70 than sites 2-4 while gill GST was higher in sites 3 and 4 than 1 and 2. Comparison with sites in the North Sea (site 5: outside Tj?rn? in The Koster archipelago in the Skagerack) and Baltic Sea (site 6: Ask? island) also revealed lower hsps 40 and 70 in site 6 (low salinity) than site 5 (high salinity) although hsps 60, 70 and 90 were detectable in digestive gland unlike sites 1-4. Previously, only hsp 70 had been studied at these sites [Mar. Environ. Res. 39. (1995), 181]. At the mRNA level, gill hsp 70 is 80-fold higher at Tj?rn? than Ask?. These data suggest that, while salinity may slightly decrease hsp 40 and 70, both hsp 70 and GST are selectively up-regulated by approx. 10- and 3-fold, respectively, at Tj?rn? compared to the other sites which we attribute to exposure to more widely fluctuating pollution levels.  相似文献   

Dynamic Analysis for Structures Isolated with Dampers     

Gu Peiying  Gao Ming M. Sc  Engineer  Material  Structure Department  Nanjing Hydraulic Research Institute  Nanjing Professor  Material  Structure Department  Nanjing Hydraulic Research Institute  Nanjing 《中国海洋工程》1996,(3)

- In this paper, mathematical models and dynamic analyses for both SDOF and MDOF structures isolated with dampers are established and performed, and a comprehensive computation method is provided. The shock absorbing effect is illustrated through an example of a two DOF damper-isolated system excited by sinusoidal waves and actual ground acceleration input recorded in earthquakes. It is shown that most of the responses of the structure with dampers reduce greatly near the resonant zone, but acceleration is enlarged in the lower or higher frequency zone; among various parameters, the influence of frictional coefficient n is the most significant, that of damping ratio of the dampers , is the second, and that of stiffness coefficient of the dampers k, is the slightest.  相似文献   

平鲷雌核发育二倍体的人工诱导          下载免费PDF全文

蔡泽平  胡超群  牛文涛  张俊彬 《海洋学报》2007,29(3):114-119

平鲷(Rhabolosargus sarba)养殖群体具有雌性生长优势的特点.应用冷休克方法进行平鲷雌核发育二倍体人工诱导,采用高温消毒的自然海水为精子稀释液,精液经强度为700μW/(cm2·s)的紫外线(UV)照射100 s,染色体遗传物质失活,卵子经激活后,可以获得95%～100%单倍体胚胎.实验结果表明:在卵子激活后5 min进行冷休克,温度0～8℃,处理时间持续15min,雌核发育二倍体的受精率达71.3%～82.7%,孵化率为24.7%～36.3%,2～6℃为适宜冷休克温度,此范围内的温度对雌核发育二倍体的受精率和孵化率没有显著影响(P>0.05),而与0,1和8℃实验组的受精率和孵化率差异均显著(P<0.05);在冷休克温度为3℃、持续时间为15 min的条件下,初始冷休克时间在4～6 min之间的受精率和孵化率均较高,分别达到60.0%～67.1%和30.6%～36.5%,其组间受精率和孵化率没有显著差异(P>0.05),而与其他实验组的孵化率存在显著差异(P<0.05);冷休克持续时间以11～19 min为宜,受精率和孵化率分别为46.9%～65.8%和31.7%～42.4%,在此时间内,冷休克持续时间对平鲷的雌核发育二倍体受精率和孵化率影响不显著(P>0.05).选用冷休克温度、初始冷休克时间和持续时间分别为3℃,5和15 min作为平鲷雌核发育二倍体的诱导条件是显著有效的.  相似文献   

异源精子诱导牙鲆雌核发育二倍体   总被引：13，自引：0，他引：13  

戈文龙  张全启  齐洁  包振民  张福玲 《中国海洋大学学报(自然科学版)》2005,35(6):1011-1016

采用紫外线照射处理的石鲽精子和受精后冷休克的方法诱导了牙鲆雌核发育。结果表明:用2只平行的相距15cm的20W紫外灯管,照射距离为10cm,在5~100s时间范围内照射石鲽精子时,受精卵的发育表现了典型的哈特维希效应,照射40s的精子所得胚胎的孵化率达到最高峰(41%),染色体观察结果显示,此照射剂量组的所有胚胎均为单倍体(n=24),表明照射精子遗传失活的有效性和可靠性。如果冷休克处理前受精卵保持在(15.5±0.5)℃,受精卵在一定的休克温度(0~10℃)条件下,用不同休克起始时间(受精后1~10min)及冷休克处理持续时间(15~90min)均能诱导染色体加倍。对冷休克处理条件的优化结果表明,在2~4℃条件下,受精后3min开始、冷休克处理45min时,二倍体诱导率最高,达79.7%。染色体鉴定显示,处理组的正常胚胎均为二倍体(2n=48),未发现单倍体和非整倍体。  相似文献   

克氏原螯虾热休克蛋白70ku类似物基因的cDNA分析     

曾勇  陆承平 《海洋科学》2006,30(6):31-34

根据已测序的克隆号PCI246基因序列设计2条引物,用快速扩增cDNA末端技术扩增克氏原螯虾(Procambarus clarkii)70 ku热休克蛋白同类物基因cDNA的5'端片段和3'端片段,测序得到1 184 bp基因片段,包含该基因的完整3'端,GenBank登录号AY357302,与GenBank序列号AB016836家蚕(Bombyx mori)的70 ku热休克蛋白同类物mRNA的部分片段83%同源。和果蝇(Drosophila melanogaster)的热休克蛋白同类物3的同源性最高(GenBank的登录号分别是NP_727 563,NP_511 132,NP_727 564,NP_727 565),为85%。  相似文献   

大黄鱼雌核发育二倍体的人工诱导   总被引：2，自引：0，他引：2  

许建和  尤锋  吴雄飞  蒋宏雷  徐永立  张培军 《海洋科学》2006,30(12):37-42

采用紫外线照射对大黄鱼(Pseudosciaena crocea Richardson)精子遗传物质进行完全灭活,灭活后精子与卵子受精获得雌核发育单倍体受精卵,再通过冷休克对单倍体受精卵进行倍性恢复,成功诱导出大黄鱼雌核发育二倍体。实验结果表明:(1)在紫外照射过程中照射剂量与大黄鱼精子和卵子受精后其成活率和孵化率呈现出明显的Hertwig效应,紫外照射时间超过2 min(剂量>2 400 ergs/mm2),可使大黄鱼精子遗传物质完全灭活。大黄鱼雌核发育诱导中精子遗传灭活的最适紫外照射时间为2.5 min(剂量为3 000 ergs/mm2);(2)冷休克法可成功抑制大黄鱼受精卵第二极体排放,诱导效果受处理时刻、处理时间和处理温度三因素的影响。综合雌核发育二倍体的诱导率、处理后受精卵早期胚胎存活率和仔鱼孵化率等因素,较适合的染色体加倍条件为23℃培育水温下授精后2 min,在3-4℃海水中处理10 min。  相似文献   

研究现今地壳运动和强震机理的一种方法   总被引：5，自引：2，他引：5       下载免费PDF全文

申重阳  李辉 《地球物理学进展》2007,22(1):49-56

本文阐述了地壳形变与密度变化耦合运动的基本理论，分析了地壳形变与密度变化耦合运动引起的重力时间变化.断层位错运动是地壳形变与密度变化耦合运动的特殊形式.利用重复重力观测数据，选用稳健算法反演研究了1985-2002年滇西地震实验场主要断裂运动的时间分布和强震响应特征，获得了不同类型强震孕育过程图像.  相似文献   

The shock event of October 6, 1597 is a strong deep earthquake     

高山泰  于岫嵋  朱大庆  李玉贤  张兴科 《地震学报(英文版)》2003,16(3):340-347

A special earth shock event was recorded at 22 counties of the 7 provinces of eastern China on October 6, 1597, and 2 volcanic eruptions and seismic activities were recorded in Sanshui county, Xianjingbeidao, Korea at that day and the 3rd day. Because of the large range of this shock, low intensity, slow attenuation and no extreme-earthquake area, its epicenter and focus could not be determined onthe scientific-technological conditions at that time. In the Seismological Catalogue of China (GU, 1983) published in 1983, its epicenter was determined to be in the Bo Sea (38.5°N, 120.0°E), its magnitude was 7; and it was changed into 7.5 in the later Seismological Catalogue of Beijing; someone estimated it over 7(HUAN, 1989); someone thought that, it was a very large earthquake, might be a extraordinarily serious large one according to the scale of sensational range, reaching 8 (SHI, et al, 1985). The authors think that, the disputes on its magnitude and epicenter, and on deep or shallow earthquake show the complicities of this problem; further discussions about it will be helpful to the study on the seismic activity of the northern China area.  相似文献   

行星际速度增幅扰动的演化   总被引：1，自引：0，他引：1       下载免费PDF全文

胡友秋  刘宝军 《地球物理学报》2003,46(3):289-294

采用二维理想MHD模型，分别在日球赤道面（二维二分量模型）和日球子午面（二维 三分量模型）内研究太阳风中纯速度增幅扰动的演化. 结果表明，该扰动在向外传播的过程 中逐渐演化为双重激波对，即由4个激波组成的激波系统. 该4个激波按离太阳由近及远依次 为后向快激波、后向慢激波、前向慢激波和前向快激波. 双重激波对在子午面内相对扰动源 中心法线基本对称，而在赤道面内则不对称：扰动源中心法线西侧双重激波对结构更为明显 ，所跨经度范围宽于东侧. 初步分析表明，行星际磁场的螺旋结构是产生日球赤道面内双重 激波对结构东西不对称性的主要原因.  相似文献   

A numerical simulation of a wind/molecular clump interaction     

A. C. Raga  J. Cantó  S. Curiel  & S. Taylor 《Monthly notices of the Royal Astronomical Society》1998,295(4):738-742

It has been pointed out in the past that it is impossible to accelerate molecular material to velocities ≥ 25 km s⁻¹ with gasdynamic shocks without dissociating the gas. Because of this, it has been argued that observations of molecular emission with radial velocities ∼ 20–100 km s⁻¹ imply the presence of 'C-shocks' (which have much lower post-shock temperatures, and therefore do not dissociate the gas) and the existence of strong (∼ 10–100 μG) magnetic fields.   In this paper, we discuss an alternative mechanism for accelerating molecular material to high velocities: a high-velocity, low-density wind drives a non-dissociative shock (with shock velocity v _cs ≤ 25 km s⁻¹) into a high-density, molecular clump. Once this shock wave has gone through the clump, the molecular material is moving at a velocity ∼  v _cs and has a gas pressure approximately equal to the ram pressure of the impinging wind. The compressed molecular clump can now be accelerated directly by the ram pressure of the wind (without the passage of further shocks through the molecular material), and will eventually move at the wind velocity.   This mechanism has been previously invoked to explain high-velocity molecular emission. However, numerical simulations have shown that a wind/clump interaction leads to the fragmentation of the clump before it can be accelerated to large velocities. In our numerical simulation (which includes an approximate treatment of the relevant microphysics) we find that the fragments that are produced are still largely molecular, and that they are rapidly accelerated to velocities comparable to the wind velocity. We therefore conclude that a wind/molecular clump interaction is indeed a valid mechanism for producing high-velocity molecular features.  相似文献