融合GNSS和加速度计监测数据的超高建筑动态特性分析     

王亚荣  黄声享  匡翠林 《测绘通报》2019,(8):14-19

GNSS和加速度计是目前动态监测超高建筑环境载荷变形的主要手段。GNSS具有无需通视、可直接获取三维位移等优点,但受精度和采样率的限制,其对微变形及高频振动信息不敏感;而加速度计具有高精度和高采样率等优点,但无法监测超高建筑低频的似静态变形。为充分发挥这两种传感器的各自优势,提出利用多速率Kalman滤波和RTS平滑方法对超高建筑GNSS和加速度计监测数据进行融合处理。试验结果表明,与单一的GNSS监测技术相比,该方法有利于削弱GNSS高频噪声的影响,提高位移数据的采样率,可有效识别超高建筑的低频和高频振动频率,提高对微变形振动的监测能力;与单一的加速度计监测技术相比,该方法可以准确监测超高建筑的低频变形信息,具有良好的工程应用价值。  相似文献   

一例异育银鲫(Carassius auratus gibelio)暴发性出血病病原分析     

袁雪梅  潘晓艺  郝贵杰  刘莉  吕孙建  蔺凌云  沈锦玉 《海洋与湖沼》2019,50(4):913-920

2016年6月,江苏某异育银鲫(Carassius auratus gibelio)养殖场暴发一种传染性急性出血病,造成养殖银鲫大量死亡。为分析此次疾病病因及流行规律,本研究从发病养殖场采集患出血病的异育银鲫,从细菌、病毒及寄生虫三个方面对病原进行了分析。采用病原菌分离、组织病理学观察、超薄切片电镜观察、病毒核酸分析、回感实验等对病原进行鉴定。结果显示从发病鲫鱼体内分离到病毒一株,未发现寄生虫及细菌感染。经测序及序列分析,该病毒为鲤疱疹病毒Ⅱ型(Cyprinid herpesvirus2,CyHV-2)病毒,组织病理学观察结果显示患病鱼的鳃和肾脏有明显病变,电镜下可观察到病鱼脾脏组织有带囊膜的球形病毒,囊膜直径约为170—200nm,病毒衣壳直径约为110—120nm,核心直径约为60nm,用组织匀浆感染鲫鱼囊胚细胞系(CGB)可稳定地观察到典型的细胞病变,用患病鱼组织匀浆液人工感染异育银鲫的死亡率高达100%,荧光定量PCR检测到该病毒可感染多器官,其中以脾脏中病毒含量最高,其次是脑,肝脏中最少。本研究可为CyHV-2的诊断防控及疫苗研制提供资料。  相似文献   

Uncertainty analysis of soil-pile interactions of monopile offshore wind turbine support structures     

《Applied Ocean Research》2019

The objective of this work is to study the uncertainties involved in the modelling of the soil-pile interaction concerning their influence on the prediction of the dynamic structural response of monopile offshore wind turbine support structures. Two main issues are identified and addressed: the adequacy of the method used to deal with the soil-pile interactions and the adequacy of the soil properties reflecting the behaviour of the soil. The present study develops an approach that defines the penetrated pile length depending on the soil profile. Also, a parameter is defined to avoid the excessive usage of steel for the penetrated pile structure. The uncertainties are included in the probabilistic free vibration analysis and the contribution of each random variable to the scatter of the response is estimated by performing a sensitivity analysis. The results indicate that the uncertainty involved in the modelling of the soil profile has a significant effect on the coefficient of variance of the natural frequency, which is a serious issue to be considered in the fatigue life assessment of offshore wind turbine support structures.  相似文献   

Nonplanar multi-modal vibrations of fluid-conveying risers under shear cross flows     

《Applied Ocean Research》2019

Risers/pipes conveying fluid are a typical kind of slender structures commonly used in marine engineering. It is of great academic significance and application value for us to evaluate and understand the vibration characteristics and nonlinear responses of these risers under the combined action of internal and external fluid flows. In this paper, the nonplanar vibrations and multi-modal responses of pinned-pinned risers in shear cross flow are numerically studied. With this objective in mind, the van der Pol wake oscillators are used to simulate the dynamical behavior of the vortex shedding in the wake. Two nonlinear equations of motion of the riser are proposed to govern the lateral responses of the riser structure. The nonplanar nonlinear equations for the riser and wake are then discretized by employing Galerkin's method and solved by using a fourth-order Runge–Kutta integration algorithm. Theoretical results show that the coupled frequencies for cross-flow (CF) and in-line (IL) motions and the corresponding coupled damping ratio could be influenced by the external and/or internal fluid velocities. Based on extensive calculations, the dynamical behavior of the riser with various internal and external flow velocities are presented in the form of bifurcation diagrams, time traces, phase portraits, oscillation trajectories and response spectrum curves. It is shown that some interesting dynamical phenomena, such as ‘lock-in’ state, ‘figure-of-eight’ trajectory and quasi-periodic oscillation, could occur in such a fluid-structure interaction system. Our results also demonstrate that the shear parameter can significantly affect the dynamic responses of the riser. When the shear parameter of the cross flow is large, multi-modal quasi-periodic responses of the riser can be excited, showing some new features undetected in the system of fluid-conveying risers in uniform cross flow.  相似文献   

Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency     

Aibin Zhan  Zhenmin Bao  Xiaoli Hu  Wei Lu  Jingjie Hu 《中国海洋大学学报(英文版)》2009,8(2):161-165

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.  相似文献   

Isolation and Characterization of Collagen from Squid(Ommastreoges bartrami) Skin     

YAN Mingyan  LI Bafang  ZHAO Xue 《中国海洋大学学报(英文版)》2009,8(2)

Collagen of squid (Ommastrephes bartrami) skin was examined in the present study. Histology showed that collagen fiber in the skin was partially cross-linked with muscle fiber. Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were extracted from the skin and characterized. The results of amino acid composition and electrophoretic patterns revealed that ASC and PSC were both type Ⅰ collagen, containing α1 and α2 chains. FTIR (fourier transform infrared spectroscopy) investigations con-firmed the existence of helical arrangements in PSC of squid skin. The denaturation temperature (Td) and shrinkage temperature (Ts)of PSC were 29.4℃ and 52.8℃, respectively.  相似文献   

Isolation and Characterization of Collagen from Squid(Ommastrephes bartrami) Skin   总被引：1，自引：0，他引：1  

YAN Mingyan  LI Bafang    ZHAO Xue College of Food Science  Engineering  Ocean University of China  Qingdao  P. R. China 《中国海洋大学学报(英文版)》2009,(2)

Collagen of squid(Ommastrephes bartrami) skin was examined in the present study. Histology showed that collagen fiber in the skin was partially cross-linked with muscle fiber. Acid-solubilized collagen(ASC) and pepsin-solubilized collagen(PSC) were extracted from the skin and characterized. The results of amino acid composition and electrophoretic patterns revealed that ASC and PSC were both type Ⅰ collagen,containing α1 and α2 chains. FTIR(fourier transform infrared spectroscopy) investigations confirmed t...  相似文献   

Experimental Study on Coupled Cross-Flow and in-Line Vortex-Induced Vibration of Flexible Risers   总被引：1，自引：1，他引：0  

郭海燕  娄敏 《中国海洋工程》2008,22(1):123-129

In this work, we study the coupled cross-flow and in-line vortex-induced vibration （VIV） of a fixedly mounted flexible pipe, which is free to move in cross-flow （ Y- ） and in-line （ X- ） direction in a fluid flow where the mass and natural frequencies are precisely the same in both X- and Y-direction. The fluid speed varies from low to high with the corresponding vortex shedding frequency varying from below the first natural frequency to above the second natural frequency of the flexible pipe. Particular emphasis was placed on the investigation of the relationship between in-line and cross-flow vibration. The experimental results analyzed by using these measurements exhibits several valuable features.  相似文献   

Hill Instability Analysis of TLP Tether Subjected to Combined Platform Surge and Heave Motions   总被引：1，自引：0，他引：1  

徐万海  曾晓辉  吴应湘  刘家悦 《中国海洋工程》2008,22(4):533-546

This paper presents the Hill instability analysis of Tension keg Platform （TLP） tether in deep sea. The 2-D nonlinear beanl model, which is undergoing coupled axial and transverse vibrations, is applied. The governing equations are reduced to nonlinear Hill equation by use of the Galerkin＇ s method and the modes superposition principle. The Hill instability charted up to large parameters is obtained. An important parameter M is defined and can be expressed as the functions of tether length, the platform surge and heave motion amplitudes. Some example studies are performed for various envirotnnental conditions. The results demonstrate that the nonlinear coupling between the axial and transverse vibrations has a significant effect on the response of structure. It needs to be considered for the accurate dynamic analysis of long TI2 tether subjected to the combined platfolna surge and heave motions.  相似文献   

落球法测量高频振动对淤泥的液化效果     

潘志芳  张丛丽  喻国良 《海岸工程》2008,27(1):41-46

为了提高疏浚工程中管道输送淤泥的效率,对淤泥预处理技术——高频微幅振动液化技术进行了初步研究。主要介绍了落球法测量淤泥粘滞系数的实验,对不同性质的淤泥分别进行实验,根据距离振动棒不同位置的小球的沉降速度求出相应位置的粘滞系数,并将其进行比较,获得液化影响范围。实验结果表明,高频振动对于粉砂质淤泥具有良好的液化效果。  相似文献