Exploring space-time models for West Nile virus mosquito abundance data     

《Applied geography (Sevenoaks, England)》2013

This paper explores variant space-time models for log-transformed West Nile virus (WNv) mosquito data, which explicitly account for both local environmental conditions and complex dependent structures. Four space-time models take various forms to accommodate correlated structure in space and time, nested data, and nonstationarity. The average WNv mosquito abundance is captured by a global trend across all four models, but different model assumptions are imposed on the stochastic component of the proposed models: a simple multivariate linear regression model with independent and identical errors, a site-specific linear mixed model with temporally correlated errors, a week-specific linear mixed model with spatially correlated errors, and a local space-time kriging model. In a case study, the predictive performance of the four models was assessed using data collected in 2007 and 2008 for the Greater Toronto Area by the mosquito surveillance program of Ontario Ministry of Health and Long-term Care: the local space-time kriging model outperforms others, but closely followed by a site-specific linear mixed model with temporal correlation. Our findings suggest that the predictive accuracy of space-time WNv mosquito abundance models can be enhanced by explicitly taking into account spatiotemporal correlation, nonstationarity, and the data collection procedure, such as surveillance design, based on sound understanding of mosquito behavior and population dynamics.  相似文献   

海洋浮游植物病毒的研究进展   总被引：6，自引：1，他引：5  

王丹  孙军  汪岷  刘东艳 《海洋科学进展》2005,23(1):105-113

海洋浮游植物病毒在海洋生态系统中,特别是在赤潮的消亡过程中起了很重要的作用。介绍了海洋浮游植物病毒的种类、多样性和丰度分布及其在海洋生态系统中的主要作用和研究现状。重点介绍和比较了海洋浮游植物病毒多样性、丰度的DNA聚合酶链扩增法(PCR)、透射电镜法、荧光显微镜法和流式细胞仪法等研究方法及其发展状况,以期为今后的研究奠定基础。  相似文献   

一种带节点移动的手机蓝牙病毒传播模型     

邱国利  蒋国平  宋玉蓉 《武汉大学学报(信息科学版)》2010,(5)

研究了手机节点的空间特性和移动特性,提出了一种新的手机蓝牙病毒传播模型。结合人类运动的行为模式,给出了移动环境下手机节点平均度的计算方法。结合渗流理论分析了蓝牙病毒大规模爆发的可能性,讨论了节点分布密度、蓝牙连接概率等参数对手机蓝牙病毒传播的影响。仿真结果表明,该模型较好地模拟了手机蓝牙病毒的传播过程,符合手机蓝牙病毒的传播规律。  相似文献   

对虾细胞培养的研究现状、存在问题及建立细胞系策略     

刘大磊  董洵  卢毅 《海岸工程》2006,25(2):107-114

近年来,对虾病毒病的爆发,使我国养殖对虾的年产量大幅度下降,造成了巨大的经济损失。就对虾细胞培养研究现状、应用前景和存在的关键问题等进行了综述和讨论。  相似文献   

咳喘宁对哮喘豚鼠IL-6、TNF-α的调节作用研究          下载免费PDF全文

杨婷  屈波  董晓斐 《应用海洋学学报》2020,36(7):144

目的：研究咳喘宁对哮喘豚鼠白细胞介素（IL-6）和肿瘤坏死因子（TNF-α）的调节作用。方法：将60只豚鼠随机分为空白组10只、造模组50只。利用呼吸道合胞病毒（RSV）感染豚鼠建立动物模型,加鸡卵白蛋白（OVA）诱导建立哮喘模型。造模组成功后再将其随机分为模型组,咳喘宁高、中、低剂量组,西药组,每组各10只。咳喘宁高、中、低剂量组分别给予不同剂量的咳喘宁口服液,西药组给予地塞米松+利巴韦林,空白组、模型组给予0.9%氯化钠注射液。经治疗7 d后,应用HE染色法检测咳喘宁对RSV哮喘豚鼠模型的治疗效果;收集支气管肺泡灌洗液（BALF）并采集心脏血,用酶联免疫吸附（ELISA）法分析血清、BALF中IL-6和TNF-α的含量。结果：血清、BALF中IL-6、TNF-α含量,模型组与空白组比较,差异有统计学意义（P＜0.05）,说明造模成功。咳喘宁高、中、低剂量组及西药组与模型组比较,差异均有统计学意义（P＜0.05）。咳喘宁高剂量组与西药组比较,差异亦有统计学意义（P<0.05）。结论：咳喘宁可以降低炎性因子水平,控制气道炎症,调节IL-6和TNF-α含量,改善哮喘模型豚鼠的肺组织病理变化。  相似文献   

对虾白斑综合症病毒极早期基因启动子筛选文库的构建     

林梵宇  徐丽美  杨丰 《台湾海峡》2010,29(2):184-188

对虾白斑综合症病毒（white spot syndrome virus,WSSV）是危害对虾养殖业的主要病原,而WSSV极早期基因对病毒功能基因的转录翻译和调控起着至关重要的作用.本研究将WSSV基因组以超声法随机断裂至400～900bp DNA片段,并插入启动子缺失且多克隆位点下游具有报告基因的载体来构建文库,转染昆虫细胞Hi5.倒置荧光显微镜观察发现部分转染后的细胞有增强型绿色荧光蛋白（EGFP）的表达,表明这部分细胞中的DNA片段可能具有极早期启动子活性.流式细胞仪分析显示,这部分荧光细胞占总细胞的比例为0.35%.由于转染效率为50%,可认为占比0.7%的WSSV基因组随机片段能在Hi5细胞中激发下游报告基因的转录翻译,可能具有极早期基因启动子活性.这对于进一步筛选和鉴定WSSV极早期基因及开展极早期启动子研究具有重要的意义.  相似文献   

Mesoscale and microscale spatial variability of bacteria and viruses during a Phaeocystis globosa bloom in the Eastern English Channel     

J.R. Seymour  L. Seuront  M.J. Doubell  J.G. Mitchell 《Estuarine, Coastal and Shelf Science》2008,80(4):589-597

Sampling was conducted within inshore and offshore sites, characterized by highly dissimilar hydrodynamic and hydrobiological conditions, in the Eastern English Channel. The eutrophic inshore site was dominated by the influence of a dense bloom of the Prymnesiophyceae phytoplankton species Phaeocystis globosa, while the offshore site was characterized by more oceanic conditions. Within each site the microscale distributions of chlorophyll a and several flow cytometrically-defined subpopulations of heterotrophic bacteria and viruses were measured at a spatial resolution of 5 cm. The inshore site was characterized by comparatively high levels of microscale spatial variability, with concentrations of chlorophyll a, heterotrophic bacteria, and viruses varying by 8, 11 and 3.5-fold respectively across distances of several centimeters. Within the offshore site, microscale distributions of chlorophyll a and bacteria were markedly less variable than within the inshore site, although viruses exhibited slightly higher levels of heterogeneity. Significant mesoscale variability was also observed when mean microbial parameters were compared between the inshore and offshore sites. However, when the extent of change (max/min and coefficient of variation) was compared between meso- and microscales, the variability observed at the microscale, particularly in the inshore site, was substantially greater. This pattern suggests that microscale processes associated with Phaeocystis globosa bloom dynamics can generate heterogeneity amongst microbial communities to a greater degree than large scale oceanographic discontinuities.  相似文献   

鱼类淋巴囊肿病毒靶器官的组织病理研究   总被引：9，自引：3，他引：9  

绳秀珍  战文斌 《中国海洋大学学报(自然科学版)》2006,36(5):749-753

探讨病毒传播途径,运用组织病理学技术,对患淋巴囊肿病毒病的牙鲆和鲈鱼的组织进行显微观察,并对病毒感染的靶器官进行了研究。结果表明,在牙鲆体表皮下结缔组织中,当大量成纤维细胞膨大、变圆,大小为10~18μm时,细胞膜外未出现囊壁,少数细胞的细胞质中观察到嗜碱性物质;18~20μm的膨大细胞在细胞膜外出现囊壁,细胞质内出现嗜碱性物质;>20μm的细胞具有了淋巴囊肿细胞的典型特征。另外,在牙鲆鳃、肠上皮下结缔组织和粘膜下层中,脾、头肾边缘组织和表面系膜、肝表面系膜,以及鲈鱼的鳃等部位均观察到淋巴囊肿细胞,并在牙鲆和鲈鱼脾内观察到膨大细胞。因此,淋巴囊肿病毒的靶器官主要是鱼体表皮下结缔组织,其次是鳃、体腔膜、肠、脾和头肾。鱼体表皮下结缔组织是淋巴囊肿病毒病的检测、检疫和早期诊断的最佳样品。  相似文献   

"红肉病"文蛤(Meretrix meretrix Linnaeus)中发现的3种病毒样颗粒   总被引：3，自引：1，他引：3  

任素莲  绳秀珍  王德秀  宋微波 《中国海洋大学学报(自然科学版)》2002,32(4):557-561

本文报道了在患“红肉病”文蛤组织中发现的 3种病毒样颗粒。 1种主要存在于鳃上皮下的结缔组织中 ,呈球形或椭球形 ,大小 (5 0～ 6 0 ) nm× (5 0～ 110 ) nm,无囊膜包裹。另 1种为球形 ,直径 16 0～ 180 nm,有囊膜 ,存在于外套膜上皮细胞及结缔组织细胞质中。第 3种存在于消化盲囊上皮细胞质内 ,为无囊膜包裹的球形病毒样颗粒 ,直径为 5 0～ 80 nm。  相似文献   

Diseases of gadoid fish in cultivation: a review   总被引：1，自引：1，他引：1  

Bricknell  Ian R.; Bron  James E.; Bowden  Tim J. 《ICES Journal of Marine Science》2006,63(2):253-266

  相似文献