Cloning and expression of prion protein encoding gene of flounder （Paralichthys olivaceus）     

张之文  孙修勤  张进兴  昝金东 《中国海洋湖沼学报》2008,26(1):50-53

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Cloning and Expression Analysis of a Prion Protein Encoding Gene in Guppy （Poecilia reticulata）     

WU Suihan  WEI Qiwei  YANG Guanpin  WANG Dengqiang  ZOU Guiwei  CHEN Daqing 《中国海洋大学学报(英文版)》2008,7(4)

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SCREENING OF AGGLUTININS IN MARINE ALGAE FROM FUJIAN COAST OF CHINA   总被引：1，自引：0，他引：1  

郑怡  卢海声 《中国海洋湖沼学报》2002,20(3):256-260

Thirty-three species of marine algae belonging to Rhodophyta, Phaeophyta and Chlorophyta from the Fujian coast were examined for agglutinins with different animal and human erythrocytes. Protein extracts from 26 species were active against at least one type of the erythrocytes tested. There were 3 species (Grateloupia imbricata, lshigefoliacea and Entermorpha prolifera) whose extracts could agglutinate all the erythrocytes used. The lowest protein concentration required to produce erythrocyte agglutination varied remarkably, from 3.1μg/ml to 500μg/ml . The strongest activity was found in the agglutina-tion of rabbit erythrocytes by Gloiopeltis furcata extract. Inhibition assays performed with nine mono- and bisaccharides indicated that agglutinations of rabbit erythrocytes by extracts of 7 species were inhibited by one or more types of the sugars assayed. The agglutinating activity shown by extracts of most species wasnot affected when the test solution was heated to 90℃, but was lost at 95℃ - 100℃. A few extracts losttheir activity at 60 RS, 65 RS and 75 RS, respectively.  相似文献   

DIETARY PHOSPHORUS REQUIREMENT OF YOUNG ABALONE, HALIOTIS DISCUS HANNAI INO     

Tan Bei-ping  Mai Kang-sen  Liufu Zhi-guo 《中国海洋湖沼学报》2002,20(1):22-31

An experiment was performed to determine the dietary phosphorus requirement of the young abalone,Haliotis discus hannai. Five semi-purified diets were formulated to provide a series of graded levels of dietary total phosphorus (0.23%–1.98) from monobasic potassium phosphate (KH2P04). The brown alga,Laminaria japonica, was used as a control diet. Similar size abalone were distributed in a single-pass, flow-through system using a completely randomized design with six treatments and three replicates each treatment. The abalone were hand-fed to satiation with appropriate diets in excess, once daily at 17:00. The feeding trial was run for 120-d. Survival rate and soft-body to shell ratio (SB/S) were constantly maintained regardless of dietary treatment. However, the weight gain rate (WGR), daily increment in shell length (DISL), muscle RNA to DNA ratio (RNA/DNA), carcass levels of lipid and protein, soft-body alkaline phosphatase (SBAKP), and phosphorus concentrations of whole body (WB) and soft body (SB) were significantly (ANOVA, P<0.05) affected by the dietary phosphorus level. The dietary phosphorus requirements of the abalone were evaluated from the WGR, DISL, and RNA/DNA ratio respectively, by using second-order polynomial regression analysis. Based on these criteria, about 1.0%–1.2% total dietary phosphorus, i.e. 0.9%–1.1% dietary available phosphorus is recommended for the maximum growth of the abalone. Project 39670572 supported by the NSFC.  相似文献   

气候变化与牲畜掉膘     

斯迪 《内蒙古气象》1998,(3):37-37

每年入冬以后,当外界温度下降到一定程度时,牲畜仅靠饲料代谢所产生的热能就不足以维持其正常体温的需要,这时畜体将消耗体内贮存的脂肪和蛋白质,表现为体重的减轻,这就是牲畜的掉膘现象。造成牲畜掉膘的原因,除与冬季牧草枯黄,数量减少,营养成分降低等有关外,气...  相似文献   

适合儿童食用的海鲜     

沈婷婷 《海洋世界》2014,(6):58-61

正海鲜含有丰富的蛋白质、钙、铁等营养素,能够为宝宝提供身体生长所需的多种营养,并有利于骨骼、牙齿的发育,所以营养学家认为适当食用海鲜有利儿童健康。海鱼、海虾肉中的蛋白质含量非常高,并且氨基酸组成与人体需要相近,是质量非常好的蛋白质。海鲜中的脂肪多以不饱和脂肪酸为主,尤其是深海鱼中还含有  相似文献   

青蛙皮肤提取物可治疗癌症？     

《海洋世界》2011,(12):10-10

据英国《每日电讯报》网站报道，英国贝尔法斯特女王大学的药剂师们在对青蛙和蟾蜍的皮肤进行研究后发现。从中提取的蛋白质对治疗癌症有积极作用。  相似文献   

关注禽流感     

《山东国土资源》2005,21(11):60-60

短短的几个月，世界各地都拉响了防治禽流感的警报，H5N1型禽流感病毒也渐渐被人们所熟悉，这种病毒表面的蛋白质分为H和N两大类，H被称为细细胞凝聚素，N被称作为神经氩酸苷酶，它们都是糖蛋白，颁布在病毒表面。  相似文献   

Selection of Yarrowia lipolytica Strains with High Protein Content from Yeasts Isolated from Different Marine Environments     

CHI Zhenming WANG Fang WANG Lin LI Jing WANG Xianghong 《中国海洋大学学报(英文版)》2007,6(4):360-364

A total of 78 Yarrowia lipolytica yeast strains from seawater, sediments, mud of salterns, the guts of marine fish, and marine algae were obtained. After the crude protein of the yeasts was estimated by the method of Kjehldahl, we found that seven strains of the marine yeasts grown in soy bean cake hydrolysate with 20 g L-1 of glucose for 48 h at 28℃ contained more than 41.0 g protein per 100 g of cell dry weight and the cell dry weight was more than 4.4 g per L of the culture. Among them, strain SWJ-1b contained the highest crude protein. The results of Biolog identification and molecular methods further confirmed that they indeed belonged to Y. lipolytica.  相似文献   

化学融雪剂对小球藻生长和生理特性的影响          下载免费PDF全文

胡水  李法云  范志平  程志辉  高群  张营 《气象与环境学报》2013,29(3):102-106

寒冷地区道路表面除雪化冰使用的化学融雪剂随着地表径流进入水体,将影响水生态系统中的水生生物正常生长,并破坏水生态系统平衡。为研究化学融雪剂对水生生物的毒性效应,分析了不同浓度有机融雪剂对小球藻生长特征、藻细胞光和色素、蛋白质及多糖含量的影响。结果表明：浓度为2 g·L^-1时有机融雪剂对小球藻生长无明显影响,当融雪剂浓度为4 g·L^-1时,小球藻细胞生长表现出明显抑制效应,且抑制效应随融雪剂浓度增加呈显著上升趋势(P<0.01);当融雪剂浓度小于4 g·L^-1时,有机融雪剂对小球藻细胞内叶绿素a合成有明显促进作用,但随着有机融雪剂处理浓度升高,藻细胞内叶绿素a含量逐渐下降。当融雪剂的处理浓度大于4 g·L^-1的时候,藻细胞内蛋白质和多糖含量与对照组相比呈显著下降趋势(P<0.01)。这说明融雪剂浓度高于4 g·L^-1时会抑制水体中小球藻正常生长繁殖,破坏藻体细胞,最终导致水生态系统平衡被破坏。  相似文献