三种海水蟹类血淋巴细胞的比较     

周晖  谢数涛 《广东海洋大学学报》2007,27(6):45-49

采用Wright’s-Geimsa混合染料对三疣梭子蟹、锈斑蜉和锯缘青蟹的血淋巴细胞进行染色，光镜下对这三种海水蟹的血淋巴细胞进行了观察和比较。根据血淋巴细胞的核质比、细胞整体着色特点、细胞质中颗粒的存在与否、颗粒的数量和分布等，三种海水蟹的血淋巴细胞均可分为颗粒细胞、半颗粒细胞和透明细胞三种主要类型，三种蟹的同类血淋巴细胞在染色形态上稍有差别。三种蟹的颗粒细胞大小差异较大，而半颗粒细胞和透明细胞大小相近。三种蟹的血淋巴细胞均以半颗粒细胞为主，透明细胞在血淋巴细胞中所占比例最小。三种蟹的血淋巴细胞密度均存在明显的雌雄差异，锈斑蜉和锯缘青蟹血淋巴细胞分类计数也存在明显的雌雄差异。  相似文献   

锯缘青蟹性腺发育与性别分化相关组织混合线性化cDNA文库构建及EST初步分析   总被引：1，自引：0，他引：1  

邹志华  张子平  王艺磊  陈锦民  贾锡伟  王淑红  林鹏 《热带海洋学报》2009,28(6):88-94

  相似文献   

Bioaccumulation of polychlorinated biphenyls in prawns and a mud crab from mixed-aquaculture ponds     

JIANG Jinhua 《海洋学报(英文版)》2009,28(6):111-117

The bioaccumulation of ten polychlorinated biphenyl (PCB) congeners was investigated within different tissues in three prawns (Penaeusorientalis Kishinouce, Laomedia astacina de Haan and Penaeus vannamei Boone) and one mud crab (Scylla serrata Forsskal) from mixed-aquaculture ponds in Taizhou in April 2007. The average concentrations (wet mass) of Σ PCBs in muscle, head, and rind tissues for mean among these prawn species were 7.04, 21.16, and 11.47 ng/g, respectively. The average concentrations of Σ PCBs in muscle, branchia, and spawn in Scylla serrata were 6.49, 21.13, and 19.76 ng/g, respectively. The concentrations of CB28 were the highest in prawn tissues (2.40 ng/g), and accounted for 18.60% of Σ PCBs. The concentration of CB52 was the lowest. The application of principal component analysis (PCA) for the estimation of PCB sources of contamination resulted in first component main contributions of CB101, CB153 and CB28 in the muscle, head, and rind in combined prawn samples, respectively. The enrichment level of Σ PCBs for different tissues varied greatly. The accumulation coefficient of Σ PCBs in prawn muscle, head, and rind tissues were 396.35, 1 191.79, and 645.91, respectively. For the Scylla serrata, the accumulation coefficients were 365.81, 1 190.50, and 1 113.31 for muscle, branchia, and spawn tissues, respectively. For prawns, the accumulation coefficient of CB153 was the highest in muscle tissues, while CB155 had the highest coefficients in the head and rind tissues. The CB155 coefficient was the highest in spawn tissues of the mud crab.  相似文献   

盐度和水流对青蟹蜕壳及存活的影响     

于忠利  乔振国  王建纲 《海洋科学》2010,34(10):68-70

在室内水泥池条件下,对平均体质量为65.3 g±1.52 g的锯缘青蟹(Scylla Serrata)进行了30 d的养殖实验,分别测定其在两种不同盐度和有无水流条件下的蜕壳率和存活率。实验结果表明,盐度15组和25组青蟹成活率差异不显著(P 0.05),但在蜕壳方面盐度15组明显快于盐度25组(P 0.05);水流对青蟹的蜕壳无明显促进作用,但有水流组的存活率显著高于无水流组(P 0.05)。因此,软壳蟹养殖应在适当降低盐度情况下,增加一定量的水流。  相似文献   

锯缘青蟹围塘养殖试验     

邝雪梅  杨文  何钢  曾锡钦  黄银姬 《广东海洋大学学报》2002,22(6):6-9

1998～ 1999年间在阳东县采用改造蟹池、水质调控、合理投饵、轮捕轮放等技术进行锯缘青蟹围塘养殖试验。结果表明 ,锯缘青蟹成活率为 70 .9% ,比 1998年提高 4 0 % ;平均产量 32 6 7kg hm2 ,比 1998年提高 12 2 .3%。 1999年与 2 0 0 0年推广养殖面积各为 10 3hm2 与 137.3hm2 ,平均产量分别为 30 5 9kg hm2 与 3132kg hm2 ,与 1998年相比分别提高 10 8.3%与 113.3%。  相似文献   

盐度胁迫对锯缘青蟹血清及组织、器官中PO和SOD活性的影响   总被引：3，自引：0，他引：3  

陈宇锋  艾春香  林琼武  徐华  沈圆虹 《台湾海峡》2007,26(4):569-575

采用酶学方法测定了盐度(5、15、25、35)胁迫下锯缘青蟹血清中酚氧化酶(PO)及其肌肉和肝胰腺中超氧化物歧化酶(SOD)活性的变化.结果表明,盐度胁迫显著影响青蟹血清PO活性(p＜0.05).S5、S15低盐度处理组青蟹血清中PO活性显著高于S25、S35高盐度处理组(p＜0.05),同一处理组青蟹血清中PO活性除S15处理组随胁迫时间延长而持续下降外,其余各组呈现出有升有降的变化.各处理组在盐度低于或高于25时,青蟹肌肉中SOD活性升高,且胁迫至72h前,随着胁迫时间延长,SOD活性下降;青蟹肝胰腺中SOD活性随着盐度升高而降低,且同一处理组青蟹肝胰腺中SOD活性则随着胁迫时间的延长而呈现出先降后升的趋势.这表明环境盐度胁迫对青蟹体内SOD活性影响显著,且表现出明显的时间效应性.由此可见,盐度胁迫显著影响青蟹的PO、SOD活性,进而影响青蟹免疫力.  相似文献   

锯缘青蟹前脑神经分泌细胞的超微结构   总被引：3，自引：0，他引：3       下载免费PDF全文

黄辉洋  李少菁  叶海辉  王桂忠 《海洋科学》2003,27(1):17-19

经透射电镜观察，锯缘青蟹（Scylla serrata)前脑具有3种类型的神经分泌细胞。I型细胞最大，胞质中存在许多大小不同的空泡，分泌颗粒数量很少；Ⅱ型细胞中等大小，细胞器发达，分泌颗粒数量较多，形态多样；Ⅲ型细胞最小，分泌颗粒数量最多，电子密度低，细胞器很少。锯缘青蟹前脑神经分泌细胞的特点，为甲壳动物系统演化处于较低的地位提供了形态学证据。  相似文献   

Effects of biogenic amines on the testicular development in mud crabs Scylla serrata     

YE Haihui  HUANG Huiyang  LI Shaojing  WANG Guizhong  LI Qifu 《海洋学报(英文版)》2006,25(2):119-124

1 IntroductionBiogenicamine, oneofim portantbioactivesub-stancesin crustaceans,participatesin extensivephysi-ologicalactivities, suchastegum entarycolorchange,m olting,m usclecontraction,glucidem etabolism,etc.(Fingerman etal.,1994).Furthermore,ithasbeen …  相似文献   

Genetic diversity and differentiation of mud crab Scylla serrata populations from southeastern China   总被引：2，自引：0，他引：2  

LI Zhongbao  LI Shaojing  WANG Guizhong 《海洋学报(英文版)》2004,23(2):309-316

The genetic diversity and differentiation of 6 mud crab Scylla serrata populations from southeastern China are investigated using allozyme electrophoresis. The mean number of alleles per locus population is 1.3 ± 0.1; the percentage of polymorphic loci per population is 27.3; the observed heterozygosity ranges from (0.195±0.083) to ( 0.241±0.090) and the expected heterozygosity ranges from ( 0.105±0.043 ) to ( 0.131±0.047 ). The coefficient of gene differentiation among populations is low (Fst =0.032), indicating that only 3.2 % of the total genetic diversity comes from inter-population, while the remaining 96.8 % comes from intra-population differences. The genetic distance among populations is 0.000-0.008 (the average is 0.002). Gene flow among the populations is large (Nm = 7.56).Genetic structure is very similar among 6 Scylla serrata populations.  相似文献   

锯缘青蟹纳精囊精子顶体酶活力研究   总被引：1，自引：0，他引：1  

管卫兵  王桂忠  李少菁  陈锦民 《海洋通报》2005,24(6):87-91

顶体酶是精子顶体部特有的胰蛋白酶,其在顶体反应、精子和透明带的结合及穿透中起重要作用.本文采用测定精子顶体氨基化酶活力方法来测定锯缘青蟹（Scylla serrata）精子的顶体酶活力.据测定,青蟹纳精囊精子项体酶活力分别为3.566 μIU/10^6和4.248 μIU/10^6.青蟹精子顶体酶活力和用同样方法测定的其它物种（如猪、人、马）相比较低.这反应了不同物种之间的精子顶体酶活力存在着较显著的差异,也反应青蟹纳精囊中精子处于一种抑制状态.这种精子活力抑制方式可能有利于精子在纳精囊中的长期存活.本实验结果也说明,临床应用的顶体酶测定方法也可以用于蟹类精液质量的评价.  相似文献