Application of Atomic Force Microscopy to Observation of Marine Bacteria   总被引：3，自引：0，他引：3  

Tomohiko Nishino  Eiko Ikemoto  Kazuhiro Kogure 《Journal of Oceanography》2004,60(2):219-225

The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as a new tool to study the fine structures of marine bacteria. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Use of an optical oxygen sensor to measure dissolved oxygen in seawater     

Hiroshi Hasumoto  Tsuneo Imazu  Toshiaki Miura  Kazuhiro Kogure 《Journal of Oceanography》2006,62(1):99-103

The use of an optical oxygen sensor to measure dissolved oxygen in seawater was investigated. The sensor is based on the dynamic quenching of an oxygen-sensitive fluorochrome embedded in the tip. Dissolved oxygen in seawater samples collected from eight stations at depths ranging from 3000 to 6000 m was analyzed both with the optical sensor and by the Winkler titration method. The two sets of data did not differ significantly. The stability and simplicity of the method and the good agreement of the results with those of the titration method indicate that the sensor would be useful for fieldwork.  相似文献   

Automated Kerogen Classification in Microscope Images of Dispersed Kerogen Preparation     

L. I. Kuncheva  J. J. Charles  N. Miles  A. Collins  B. Wells  I. S. Lim 《Mathematical Geosciences》2008,40(6):639-652

We develop the classification part of a system that analyses transmitted light microscope images of dispersed kerogen preparation. The system automatically extracts kerogen pieces from the image and labels each piece as either inertinite or vitrinite. The image pre-processing analysis consists of background removal, identification of kerogen material, object segmentation, object extraction (individual images of pieces of kerogen) and feature calculation for each object. An expert palynologist was asked to label the objects into categories inertinite and vitrinite, which provided the ground truth for the classification experiment. Ten state-of-the-art classifiers and classifier ensembles were compared: Naïve Bayes, decision tree, nearest neighbour, the logistic classifier, multilayered perceptron (MLP), support vector machines (SVM), AdaBoost, Bagging, LogitBoost and Random Forest. The logistic classifier was singled out as the most accurate classifier, with an accuracy greater than 90. Using a 10 times 10-fold cross-validation provided within the Weka software, we found that the logistic classifier was significantly better than five classifiers (p<0.05) and indistinguishable from the other four classifiers. The initial set of 32 features was subsequently reduced to 6 features without compromising the classification accuracy. A further evaluation of the system alerted us to the possible sensitivity of the classification to the ground truth that might vary from one human expert to another. The analysis also revealed that the logistic classifier made most of the correct classifications with a high certainty.  相似文献   

An investigation of subglacial processes at the microscale from Briksdalsbreen, Norway     

JANE K. HART 《Sedimentology》2006,53(1):125-146

The subglacial processes at Briksdalsbreen, Norway, are examined by a combination of sedimentology, thin section and scanning electron microscope (SEM) analysis of till samples from an exposed subglacial surface and from beneath the glacier. Studies of a fluted surface indicate that subglacial deformation is occurring on a field scale with flutes forming behind most clasts 0·6 m high. At the thin section scale (0·014–2·0 mm) it is seen that deformation is by rotation and attenuation and is dependent on till texture. At the SEM scale (0·1–0·4 mm) it is seen that erosion is controlled by abrasion and percussion which produces distinct grain ‘styles’ as part of an erosional continuum. Overall it is shown that rotation and attenuation is a dominant process at all scales and that the clast interactions associated with different scale perturbations within the shear zone control erosion and deposition, as well as landform and fabric production.  相似文献   

量子磁力仪研究和开发近况   总被引：11，自引：1，他引：11  

张昌达 《物探与化探》2005,29(4):283-287

介绍了核子旋进磁力仪、欧弗豪泽磁力仪、光泵磁力仪、超导磁力仪和原子磁力仪研究和开发近况, 提出了开展研究工作的建议。  相似文献   

ODP204航次天然气水合物的可能有利储层——浊积层   总被引：3，自引：1，他引：3  

龚建明  张莉  陈建文  Young-Joo LEE  王红霞  闫桂京 《现代地质》2005,19(1):21-25

为了解岩性在天然气水合物分布中的作用, 利用岩矿鉴定、扫描电镜和能谱法对ODP204航次的 4个站位 10个钻孔共计 115个沉积物样品进行分析, 发现沉积物中矿物成分基本相近, 以粘土矿物为主, 含少量以石英为主的陆源碎屑。样品中含有丰富的硅藻和有孔虫等微体化石, 可形成硅藻粘土—硅藻土、有孔虫硅藻土或凝灰质硅质生物粘土。石英碎屑大多呈棱角状, 粒径小、含量少, 同时, 样品中可见透镜体、团块或旋涡状层状结构以及杂乱堆积的斑杂构造。上述特征表明沉积物为含有大量深海或半深海的浊流沉积物或其夹层。由于钻井岩心中有相当部分的天然气水合物样品分布在上述沉积物中, 推断这些浊流沉积物很可能是ODP204航次天然气水合物的有利储层。  相似文献   

西北地区空中云水资源的时空分布特征   总被引：12，自引：6，他引：12  

陈勇航  黄建平  陈长和  张强  冯建东  金宏春  王天河 《高原气象》2005,24(6):905-912

利用1983年7月至1998年12月国际卫星云气候计划ISCCP D2的月平均云资料,对西北地区空中云水资源的时空分布特征进行了系统的研究。结果表明:三个不同区域的月平均总云量、光学厚度和云水路径的区域平均值分别在52.5%~58.3%,2.6~6.6和44.9~77.6 g.m-2之间;西北地区空中云水资源多年平均分布有其沿地形分布的特点,总云量、中云量、总光学厚度和总云水路径的高值区均在天山、昆仑山、祁连山一带,而低值中心一般分布在塔里木盆地—内蒙古西部戈壁沙漠—黄土高原西北部一带。此外,祁连山、青海所在的高原气候区云水资源近年呈上升趋势,特别是总光学厚度和总云水路径15年来呈明显上升趋势,分别约上升了0.8和16.4 g.m-2。  相似文献   

大气遥感与卫星气象学研究的进展与回顾   总被引：25，自引：4，他引：25       下载免费PDF全文

吕达仁  王普才  邱金桓  陶诗言 《大气科学》2003,27(4):552-566

大气遥感作为大气科学中的重要基础与技术支柱,是20世纪60年代以来迅速发展的一门年轻学科.中国大气科学工作者在这方面也紧密结合国家需求与国际学术前沿,从20世纪60年代开始在理论研究,技术发展与实验研究,以及在气象、环境和国防应用等多方面开展了活跃的研究与发展工作.作者主要对中国科学界在大气遥感与卫星气象学发展方面作简要回顾,并着重介绍中国科学院大气物理研究所在大气遥感的基础与方法研究方面的一些进展,以及对大气遥感发展中的作用.主要内容包括:中国大气遥感的简要发展过程、卫星气象的早期发展、卫星红外遥感的基  相似文献   

利用数字图像处理技术提高地震剖面图像信噪比   总被引：1，自引：2，他引：1  

陈凤  李金宗  黄建明  李冬冬 《地球物理学进展》2003,18(4):758-764

提出了利用数字图像处理技术提高地震剖面信噪比的新方法，首先根据数字图像处理要求的格式，对地震剖面数据进行转换，得到地震剖面图像，分析了地震数据特点和初步地震图像的实验结果后，设计了新的预处理方法——“二维沿层滤波”，在此基础上，利用可以计算帧间运动速度及其变化都较大的改进的光流分析技术，计算出多幅地震剖面对应点的偏移量，然后应用图像积累技术对这多幅地震剖面进行积累，实现对三维地震数据体提高信噪比的处理，该方法充分利用了三维地震信息，不但可以提高整个数据体的信噪比，而且可以减少信号能量的损失，并保持原来的信号能量关系，使地震剖面的质量得到明显提高，为地震解释奠定良好的基础。  相似文献   

用几何光学模型对方向变化和直方图建模(英文)     

ＤａｖｉｄＬ．Ｂ．Ｊｕｐｐ 《遥感学报》1997,(Z1)

森林和林地的图象二向性反射函数是一个统计函数，较之小尺度的树冠，它更多地用于大尺度的均匀覆盖的地块。用航空象片和高空间分辨率扫描仪数据作的图象热点影响研究在小尺度下显示出很大方差，而且，太阳和观测角度的交互变化进一步增加了这种反射各向异性变化的方差并有规律地继续呈现在分辨率低的图象中，这被称作ＢＲＶＦ或二向性反射方差函数。近年来，作为一种解释结构的手段，高分辨率图象的方向性方差和直方图结构越来越受到重视，这方面的数据也越来越多。这项工作是利用图象方差来解释结构问题（Ｓｔｒａｈｌｅｒ和李小文倡导）的一种延伸，并在过去１５年中由众多人员作了大量工作。在树冠尺度下，森林的直方图和二向反射方差函数可以计算出来，这里利用了近似迭代函数来处理这些数据并和数值积分模拟进行了对比，结果显示可对二向性反射方差函数的测量和引入直方图的各向异性进行准确建模。  相似文献