栉孔扇贝(Chlamys farreri)壳顶幼虫血细胞分布的免疫学观察     

倪永庆  邢婧  林听听  战文斌 《中国海洋大学学报(自然科学版)》2012,42(6):72-76

研究组前期实验发现,栉孔扇贝血细胞在D形幼虫时期出现.本文应用单克隆抗体的免疫组化、免疫电镜方法进一步定位栉孔扇贝壳顶幼虫的血细胞并观察其分布.结果表明,栉孔扇贝壳顶幼虫已分化出界限明显的组织器官,包括外套膜、面盘、口、食道、胃、消化腺、足等;血细胞主要分布于壳顶幼虫的外套膜、面盘、食道、消化腺、胃等组织器官内及周围,其中面盘、消化腺、胃等处有大量血细胞成簇分布.血细胞形状不规则,直径为3～5μm;细胞核多为圆形、椭圆形,位于细胞的一侧;细胞质内有线粒体、内质网等细胞器和空泡.结果证实,栉孔扇贝在壳顶幼虫时期已出现了大量清晰可辨的血细胞,其分布特点与成贝血细胞相似.  相似文献   

POLYCULTURE OF SCALLOP CHLAMYS FARRERI AND KELP LAMINRIA JAPONICA IN SUNGO BAY     

方建光  孙惠玲  燕敬平  匡世焕  李锋  GaryF.Newkirk  Jon Grant 《中国海洋湖沼学报》1996,(4)

Several polyculture models of scallop Chlamys farreri and kelp Laminaria japonica currently employedin Sungo Bay and other parts of northern China are described in this paper. Economic benefits of differ-ent polyculture models are analysed based on the growth rate, culture density and market price. In addi-tion, site selection, critical environmental conditions and polyculture problems are discussed in detail.  相似文献   

硒化壳聚糖对栉孔扇贝血细胞中部分免疫因子的影响     

王宜艳  孙虎山  刘玉坤 《海洋通报》2007,26(2):117-120

取人工养殖栉孔扇贝(Chlamys farreri)的血淋巴,体外加入不同浓度的硒化壳聚糖,作用1h后采用生化方法分别测定栉孔扇贝血细胞中的髓性过氧化物酶(MPO)和非特异性酯酶(NSE)的活力,以及过氧化氢(H2O2)和活性氧的含量。结果表明:加入硒化壳聚糖后,栉孔扇贝血细胞中依赖卤素的MPO、不依赖卤素的MPO、α-醋酸萘酯酶和α-丁酸萘酯酶的活力均为实验组显著高于对照组,过氧化氢和活性氧的含量也为实验组显著高于对照组。说明硒化壳聚糖对栉孔扇贝血淋巴中多种参与免疫防御的酶的活力和具有杀菌作用的免疫因子的含量均具有明显的提高作用,因而硒化壳聚糖对栉孔扇贝的免疫功能具有明显的增强作用。  相似文献   

养殖扇贝大面积死亡的原因及对策   总被引：8，自引：2，他引：8  

王远隆  李美真  邱兆星  张豫  王健伟 《海洋湖沼通报》2001,(3):63-69

养殖扇贝大面积死亡的主要原因：养殖量超过了养殖容量，种质退化，赤潮，富营养化，极端的理论环境，疾病等。针对以上原因提出了合理规划养殖面积及密度，推行贝类健康养殖技术；进行扇贝选育种；加强海洋环境监测、病害防治等对策。  相似文献   

Shell growth of sea scallops (Placopecten magellanicus) in the southern and northern Great South Channel, USA     

Harris  Bradley P.; Stokesbury  Kevin D.E. 《ICES Journal of Marine Science》2006,63(5):811-821

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An efficiency model of a scallop (Pecten maximus, L.) experimental dredge: Sensitivity study   总被引：1，自引：0，他引：1  

Fifas  S.; Berthou  P. 《ICES Journal of Marine Science》1999,56(4):489-499

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自然环境下虾夷扇贝(Patinopecten yessoensis)体内麻痹性贝毒的净化研究     

宋涛  刘磊  宋晓萍  梁玉波  庄国宏 《海洋学报(英文版)》2015,34(12):170-174

为探究在自然环境中虾夷扇贝Patinopecten yessoensis体内麻痹性贝毒(PSTs)的净化过程,从黄海北部大窑湾海域采集了受PSTs污染的虾夷扇贝,将其转移至黄海的棋盘磨水域,该水域未曾有PSTs污染的报道。本研究采用高效液相色谱-荧光检测法(HPLC-FLD)检测30天净化过程中虾夷扇贝体内PSTs的组分和水平。研究结果发现,在第9天,虾夷扇贝软体中的PSTs的含量下降到一个相对较低的水平。而且,初期扇贝体内PSTs的净化率高于后期。扇贝各组织器官中PSTs含量分析发现,消化腺中的PSTs含量最高,这对于人类健康和扇贝养殖具有重要的意义。在本研究中还发现,虾夷扇贝的死亡率与PSTs的水平有一定的关系。  相似文献   

SUMMARY OF STUDIES ON CLOSED-POLYCULTURE OF PENAEID SHRIMP WITH FISHES AND MOLUSCANS   总被引：1，自引：0，他引：1  

李德尚  董双林 《中国海洋湖沼学报》2000,18(1):61-66

ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｗｏｒｌｄ’ｓｓｈｒｉｍｐｃｕｌｔｕｒｅｉｎｄｕｓｔｒｙｈａｓｂｅｅｎｓｕｆｆｅｒｉｎｇｆｒｏｍｖｉｒａｌｅｐｉｄｅｍｉｃｄｉｓｅａｓｅｓａｎｄｆｅｌｌｉｎｔｏａｄｉｆ ｆｉｃｕｌｔｐｏｓｉｔｉｏｎｆｏｒｙｅａｒｓ.Ｉｔｉｓｇｅｎｅｒａｌｌｙａｃｃｅｐｔｅｄｔｏｄａｙｔｈａｔｔｈｅｄｉｓｅａｓｅｓｗｅｒｅｃａｕｓｅｄｍａｉｎｌｙｂｙｗａｔｅｒｐｏｌｌｕ ｔｉｏｎｃｒｅａｔｅｄｂｙｓｈｒｉｍｐｃｕｌｔｕｒｅｉｔｓｅｌｆ,ｂｏｔｈｉｎｓｈｒｉｍｐｐｏｎｄｓａｎｄｉｎｃｏａｓ…  相似文献   

Response of phytoplankton to multispecies mariculture: a case study on the carrying capacity of shellfish in the Sanggou Bay in China   总被引：3，自引：0，他引：3  

GE Changzi  FANG Jianguang  SONG Xief  MAO Yuze  YE Naihao 《海洋学报(英文版)》2008,27(1):102-112

A multispecies model for shellfish polyculture in the Sanggou Bay in China used for large-scale long-line cultivation of the Chinese scallop Chlamys farreri, the Pacific oyster Crassostrea gigas and the kelp Laminaria japonica is presented. The model includes key physical processes which are the transports of matter at the system boundary, and the main biological process that is the primary production and nutrients release from the bottom. By the model, the seasonal fluctuations of phytoplankton biomass and dissolved inorganic nitrogen(DIN) in 1994 are simulated. Furthermore, if the kelp culture scale is kept constant and the Chinese scallop and the Pacific oyster culture scales are adjusted, virtual shellfish farms are funded and responses of phytoplankton to the large-scale shellfish culture are simulated. According to these simulated results, the room limitation, and the hypothesis that shellfish will not grow well if the phytoplankton biomass is less than 8.2 mg/m³, the expandable multiple of scallop culture k and that of oyster culture y are determined as k=-0.276 5y+4.690 5 and 0.133 3k+0.006 6y ≤ 0.667 5, where, k (or y) is equal to 1, the culture scale of scallop (or oyster) is 8.8×10⁹ individuals (or 66 ha, with a density of 59 ind./m²), and the kelp culture scale is 3 300 ha with a density of 12 ind./m².  相似文献   

Ingestion of Domoic Acid and Its Impact on King Scallop（Pecten maximus,Linnaeus 1758）     

LIU Hui Maeve S. Kelly Dirk A. Campbell DONG Shuanglin ZHU Jianxin WANG Sufeng 《中国海洋大学学报(英文版)》2007,6(2):175-181

A simple method for spiking formulated feed with domoic acid （DA） was developed in this study. DA feed was prepared by mixing 0.15 mL 100 μg mL-1 DA with 0.1 g formulated feed, and drying the mixture at room temperature for 2 h. The prepared DA feed contained 0.19 pg DA per particle. Of the added DA, 46.72% was retained in the feed. Relatively high DA retention （about 50%） was recorded after DA feed was soaked in water for 2h. Exposure to DA feed for 7d did not cause the increase of tissue DA level of adult king scallop （Pecten maximus） significantly in 60 d. The increase of their gonad index after DA exposure was not significantly different from the control. No significant change in DA level was found in spermary, ovary or fertilized eggs after DA exposure. These results indicated that DA excretion may be more efficient than DA accumulation under the current experimental conditions, and the mechanism of domoic acid incorporation in P. maximus may involve intracellular biotransformation.  相似文献