不同方法制备的海星皂甙细胞毒性评价     

文震  党志  宗敏华  赵金华  李靖 《海洋通报》2006,25(6):26-29,46

考察超临界CO2萃取、乙醇萃取两种方法制备的南海产多棘海星皂甙的溶血过程,采用分光光度法测定溶血指数,结合红细胞形态血观察,评价其细胞毒性。结果显示,两种方法制备的海星皂甙都具有明显溶血作用,超临界CO2萃取、溶剂萃取的海星皂甙溶血指数分别为19231,12821,超临界CO2萃取的海星皂甙保持了更强的细胞毒性,这为海星皂甙制备工艺的选择,探索其物质基础提供了参考依据。  相似文献   

In vitro acute cytotoxicity of the neonicotinoid insecticide acetamiprid to FG cells, the gill cell line of flounder Paralichthy olivaceus     

SU Feng ~    ZHANG Shicui ~ *  YANG Ming ~  LI Hongyan~ . Department of Bioengineering & Pharmaceutics  Qingdao University of Science & Technology  Qingdao  China . Department of Marine Biology  Ocean University of China  Qingdao  China 《海洋学报(英文版)》2006,25(5)

1 IntroductionAcetamiprid (( E)-N1-[( 6-chlor-3-pyridyl )methyl]-N2-cyano-N1-methylacetamidine), a cyano-substituted neonicotinoid compound ( Kagabu,1999), is a relatively newclass of insectide, exhibi-ting a broad insecticidal spectrum ( Matsunaka,2000; …  相似文献   

Simulations of water movement and solute transport through different soil texture configurations under negative‐pressure irrigation          下载免费PDF全文

Jiajia Wang  Yuanfang Huang  Huaiyu Long  Sen Hou  An Xing  Zhongxiang Sun 《水文研究》2017,31(14):2599-2612

This study examined the effects of different soil texture configurations on water movement and solute transport to provide a reliable scientific basis for the application of negative‐pressure irrigation (NPI) technology. HYDRUS‐2D was used to analyse water movement and solute transport under NPI. The main results are as follows: (a) HYDRUS‐2D can be used to simulate water movement and solute transport under NPI, as there was good agreement between the simulated and measured values for water contents, NaCl concentrations, cumulative water infiltration, and wetting distances in the horizontal and vertical directions; the Nash–Sutcliffe efficiency coefficients were in the range of 0.94–0.97. (b) Layered soils have obvious effects on water movement under NPI. With the emitter position in the loam layer, when a coarse texture of loamy sand was present below the loam layer (namely, L‐LS), irrigation water accumulated in the topsoil, and this led to an increase in evaporation compared with the homogeneous loam profile. However, fine texture silty loam or silty clay loam layers beneath the loam layer (namely, L‐SiL or L‐SiCL, respectively) was more conducive to water infiltration into the lower layer, and this increased the amount of water infiltration and simultaneously reduced the surface evaporation effectively. (c) Layered soils have obvious effects on solute transport under NPI, and salt accumulation will readily occur in the clay‐rich soil layer at the interface. The maximum soil salt accumulation of L‐LS occurred above the soil interface between the two soil layers with a value of 21.80 g/kg; however, for L‐SiCL and L‐SiL, the maximum salt accumulation occurred below the soil interface between the two soil layers, with values of 23.80 g/kg and 20.08 g/kg, respectively. (d) Interlayered soils showed remarkable changes in the water infiltration characteristics and salt‐leaching intensities under NPI, and the properties for the soil profile with a silty loam interlayer were better than those for the soil profile with a silty clay loam interlayer. The soil profile with a loamy sand interlayer had the lowest amount of water infiltration, which resulted in reductions of the salt‐leaching intensities. Thus, NPI is clearly not suitable for loamy sand soil. Overall, the results demonstrated that soil texture configurations affected water movement and solute transport under NPI. Therefore, careful consideration should be given to the use of NPI to achieve target soil water and solution conditions and reduce water loss.  相似文献   

Study of evolution of shear band systems in sand retained by flexible wall     

Danuta Leniewska  Zenon Mrz 《国际地质力学数值与分析法杂志》2001,25(9):909-932

The present paper is concerned with the study of the evolution of regular patterns of shear bands observed experimentally (cf. Milligan, 1974), and provides an extension of the previous work published by the same authors. The purpose of this paper is to present an improved version of a simple theoretical model, derived basically from a classical equilibrium of the Coulomb wedge. This model constitutes a modified version of an extension of the classical Coulomb wedge analysis by assuming that soil parameters are varying during the deformation process and the initial configuration at which limit equilibrium occurs evolves toward a new equilibrium configuration. The application of the model in the analysis of shear band pattern observed in dredged model tests on cantilever walls provided realistic simulation of consecutive shear band formation. Copyright © 2001 John Wiley & Sons, Ltd.  相似文献   

钢护筒护壁人工挖孔在海上基桩施工中的应用     

何开兆 《南方国土资源》2002,15(4):51-54

文章通过深能钦州码头油库工程海上码头引桥桩施工的工程实例 ,阐述钢护筒护壁人工挖孔在海上基桩施工的基本工序及施工工艺 ,证实钢护筒护壁人工挖孔施工工艺 ,在地层简单的浅海场地 ,是一种行之有效且无污染、机具简单、成本低、进度快的施工方法  相似文献   

Hydrothermal studies in a new diamond anvil cell up to 10 GPa and from −190°C to 1200°C     

W. A. Bassett  A. H. Shen  M. Bucknum  I-Ming Chou 《Pure and Applied Geophysics》1993,141(2-4):487-495

The new hydrothermal diamond anvil cell (HDAC) has been designed for optical microscopy and X-ray diffraction at pressures up to 10 GPa and temperatures between –190°C and 1200°C. Laser light reffected from the top and bottom anvil faces and the top and bottom solid sample faces produce interference fringes that provide a very sensitive means of monitoring the volume of sample chamber and for observing volume and refractive index changes in solid samples due to transitions and reactions. Synchrotron radiation has been used to make X-ray diffraction patterns of samples under hydrothermal conditions. Individual heaters and individual thermocouples provide temperature control with an accuracy of ±0.5°C. Liquid nitrogen directly introduced into the HDAC has been used to reduce the sample temperature to –190°C. The - phase boundary of quartz has been used to calculate the transition pressures from measured transition temperatures. With this method we have redetermined 5 isochores of H₂O up to 850°C and 1.2 GPa at which the solution rate of the quartz became so rapid that the quartz dissolved completely before the - transition could be observed. When silica solutions were cooled, opal spherules and rods formed.  相似文献   

扬州港客运大楼地基加固与边坡围护     

颜万才 《江苏地质》1998,22(4):231-237

位于长江下游北岸某凹岸区的扬州港,因受局部塌岸的危害,多处建构物被毁,新落成的客运大楼产生不均匀沉降与裂缝,直接危及大楼的安全与使用,迫使该港长航客运中断。后在大楼西南侧岸边构筑了一道防护帷幕墙,并对大楼地基土进行了压密注浆加固,保住了大楼,控制了沉降,并使大楼处于稳定状态,恢复了长航客运。简述了该建筑物病害的成因,治理加固方案的设计与施工,效果验证等问题。  相似文献   

九孔鲍外套膜表皮细胞的超微结构     

杜晓东 《广东海洋大学学报》1998,(3)

利用透射电镜观察九孔鲍（Haliotidaediversicoloraquatilis）的外套膜表皮细胞,结果表明细胞可分为4大类,即普通柱状表皮细胞、嗜碱性粒细胞、嗜酸性粒细胞和感觉细胞。它们在不同区域的分布、形态和数量变化与外套膜的功能分化密切相关。  相似文献   

基于双偏振雷达资料对南海弱台风降水微物理结构的分析     

王睿  黄燕燕  伍志方  林青  周浪  陈超  吴林 《热带气象学报》2022,38(1):43-57

有些南海弱台风在登陆广东时,由于路径复杂、移动缓慢,会对广东地区造成较长时间和较大范围的风雨灾害。使用双偏振雷达对2018—2020年登陆广东的南海弱台风分析,发现南海弱台风在登陆前强降水区主要有两个:一个是位于海上的台风中心南侧眼墙的降水区,另外一个是在台风移动方向的右前方,台风螺旋雨带上岸的区域。在眼墙中,Z_H和K_DP的大值区在低层同位相,Z_DR大值区位于偏上风方向,降水粒子在移动的右侧开始激发,移动的右侧至右前侧为浓度较大的小粒子降水,而右侧和右后侧为大粒子降水。而且台风降水粒子在海洋和陆地有明显差异,陆地由于地形摩擦和抬升作用,降水粒子浓度较大,但水汽和能量供应不足,降水粒子直径较小;海面由于水汽和能量供应充足,对流发展较高,主要为大雨滴的对流降水,但降水粒子浓度不及陆地。   相似文献   

HeLa细胞突起中微丝束的纳米分辨荧光成像     

陈丹妮  刘磊  于斌  牛憨笨 《海洋学报》2010,32(10):6948-6954

在Matlab编程环境下模拟了单分子定位显微的纳米分辨成像,在传统实验参数条件下,对不同间隔分子带模型进行了模拟成像.模拟结果表明,单分子定位显微方法可以区分中心相隔20 nm的两个分子带.同时,也分析了不同像元大小对单分子定位精度的影响.此外,通过单分子定位显微方法在IX71倒置荧光显微镜上实现了纳米分辨,系统极限分辨率,即半高全宽为48 nm.在该系统上,获得了HeLa细胞突起中微丝束结构的纳米分辨图像.从重构获得的图像中可以看到微丝束的直径为75—200 nm.  相似文献