自组装膜进样质谱系统及其在砂质沉积物异化硝酸盐还原研究中的应用     

谢成军  宋国栋  刘素美  唐继尧  张桂玲 《海洋学报》2020,42(2):22-29

沉积物中的异化硝酸盐还原过程对于海洋氮循环起着至关重要的作用。基于15N标记的培养技术是目前测定沉积物异化硝酸盐还原的主要手段。准确快速测定15N标记的产物(29N2、 30N2)是量化异化硝酸盐还原各个过程速率的关键。本研究自行组装膜进样质谱系统用于29N2和30N2的测定,对其测量条件进行了优化。结果表明,进样蠕动泵进样流速0.80 mL/min,进样时间3~3.5 min,恒温槽温度20~25℃,同时铜还原炉温度在300~600℃的条件下,^29N2/^28N2和^30N2/^28N2的测试精密度分别可以控制在0.1%和1%以内,比较适合29N2和30N2的测定。利用自组装的膜进样质谱系统结合15N标记的培养技术研究了青岛石老人沙滩沉积物中的异化硝酸盐还原过程。石老人沙滩沉积物不存在将硝酸盐完全还原为氮气好氧的反硝化。厌氧铵氧化、厌氧反硝化和异化硝酸盐还原为铵(Dissimilatory Nitrate Reduction to Ammonium,DNRA)的潜在速率(以湿沉积物N计)分别为(0.05±0.01) nmol/(cm^3·h),(2.32±0.21) nmol/(cm^3·h)和(1.02±0.15) nmol/(cm^3·h)。厌氧反硝化是硝酸盐异化还原主要的贡献者,其比例接近70%,其次是DNRA,比例可达30%,而厌氧铵氧化的贡献最低,仅为1%。在N2产生过程中,主要贡献者是反硝化,厌氧铵氧化的贡献仅为2%。  相似文献   

防渗膜渗漏偶极子检测的应用     

杨萍  孔波  姜余祥  王燕妮 《物探与化探》2014,(6)

偶极子法可用于防渗膜渗漏检测。膜上介质厚度以及偶极子与膜的距离会对偶极子检测的灵敏度产生影响。基于填埋场防渗膜高压直流电法的检测原理,对膜上介质厚度对偶极子检测的影响进行了研究,结果表明:防渗膜上介质厚度增大,通过在介质表面上移动偶极子来检测漏洞是困难的;但若偶极子能逼近膜的表面进行检测,无论膜上介质厚度多大,在漏洞附近都能检测到明显的电势异常。  相似文献   

边界问题及其对真空预压的影响     

高志义  冯军 《岩土工程技术》2014,28(5):250-256

给出了真空预压区四周开敞式与封闭式边界定义、两者的不同,分析了开敞式边界加固效果优于封闭式边界的原因并以三个实例说明之,给出如何将封闭式转化为开敞式的方法;柔性与刚性密封膜同样可构成开敞式与封闭式边界,讨论了此时两种边界下差别;给出了密封墙和支挡墙的定义、功能、应用范围;讨论了水泥搅拌与旋喷桩单体墙与格栅墙的定义、功能、应用范围,前者只能作为密封墙,后者既可作密封墙也可作支挡墙.  相似文献   

海洋沉积物中现存微生物化学标志物完整极性膜脂研究进展   总被引：3，自引：0，他引：3  

姚鹏  于志刚 《地球科学进展》2010,25(5):474-483

完整极性膜脂作为活的微生物细胞的化学标志物,能够反映海洋沉积物中现存微生物群落结构和生物量等信息.与生物学方法相比,完整极性膜脂分析技术具有无需培养、快速和普适性等特点.综述了海洋沉积物中细菌和古菌的细胞膜完整极性膜脂的组成特点及其在生物地球化学和微生物生态学等研究中的应用,重点评述了在生物地球化学循环中有特殊作用的微生物,如厌氧氨氧化细菌、甲烷氧化古菌、氨氧化古菌、具有四醚膜脂结构的海洋泉古菌等,或者是一些特殊生态系统,如冷泉、海底深部生物圈等研究中完整极性膜脂应用的进展.还简要介绍了完整极性膜脂的分析方法,并对其应用前景进行了展望.  相似文献   

IMPROVEMENT OF SANDY SOIL WITH WATERCONSERVING MEMBRANE AND ITS EFFECT ON CROP GROWTH   总被引：1，自引：0，他引：1  

LIXiu-jun CUIXiang-hao LIQu-sheng 《中国地理科学(英文版)》2005,15(1):64-69

1INTRODUCTIONArid, semi-arid, and semi-humid areas abound in sandysoil, which causes water to leak into deep soil orgroundwater so that soil′s water-conserving capabilitybecomes lower and water is used with low efficiency.Water is one of the important fa…  相似文献   

缘于任意自旋场的Vaidya黑洞的熵(英文)     

SHEN Yougen GAO Changjun 《中国科学院上海天文台年刊》2003,(1)

用在brick模型基础上发展起来的membrane模型 ,计算了缘于任意自旋场的非静态黑洞———Vaidya黑洞的自由能和熵。结果表明 ,玻色子场 (自旋s =1,2 )和费米子场 (自旋s =1/2 )的熵都恰好与黑洞的视界面积成正比 ,这与静态和稳态的情况相同。而且 ,玻色子和费米子场的熵具有相同的形式 ,二者相差一个系数  相似文献   

对虾白斑综合症病毒膜蛋白VP41A的功能研究     

周娟  熊生良  杨丰 《台湾海峡》2011,30(4):512-516

对虾白斑综合症病毒（WSSV）是对虾养殖的主要病害之一,在虾养殖业中造成了严重的损失．本研究从WSSV基因组中克隆到vp41a基因,大小约为900bp,发现该基因编码蛋白VP41A．通过构建重组质粒pET．His一卅J0表达了重组蛋白VP41A,大小约为43kDa．并通过Ni一琼脂糖珠交联下拉实验,在虾血细胞中找到一个能与VP41A相互作用蛋白,质谱分析发现该蛋白为细胞粘连蛋白,说明在WSSV感染宿主细胞的过程中蛋白VP41A可能发挥重要作用．本研究结果推动了WSSV与宿主对虾相互作用的研究,有利于探索病毒复制或转录的关键蛋白,为最终的病毒防治及快诛诊断提供科学依据．  相似文献   

副溶血弧菌（Vibrioparahaemolyticus）tdh2基因和鳗弧菌（Kanguillarum）ompU基因二联DNA疫苗制备及其对大菱鲆（Scophthalmusmaximus）免疫保护作用     

刘瑞  赵明君  杨慧  陈吉祥 《海洋与湖沼》2011,42(4):580-586

采用基因工程的方法,将副溶血弧菌的热稳定直接溶血素基因tdh2和鳗弧菌外膜蛋白基因ompU进行融合,在大肠杆菌中得到表达,并利用该融合基因构建二联DNA疫苗pEGFP．N1／tdh2-ompU。用DNA疫苗按10和50μg／尾的剂量通过肌肉注射免疫大菱鲆,对大菱鲆抵抗致病性副溶血弧菌和鳗弧菌的免疫效果进行研究。结果表明...  相似文献   

5. Prebiotic Chemistry – Biochemistry – Emergence of Life (4.4–2 Ga)     

Robert Pascal  Laurent Boiteau  Patrick Forterre  Muriel Gargaud  Antonio Lazcano  Purificación Lopez-Garcia  Marie-Christine Maurel  David Moreira  Juli Pereto  Daniel Prieur  Jacques Reisse 《Earth, Moon, and Planets》2006,98(1-4):153-203

This chapter is devoted to a discussion about the difficulties and even the impossibility to date the events that occurred during the transition from non-living matter to the first living cells. Nevertheless, the attempts to devise plausible scenarios accounting for the emergence of the main molecular devices and processes found in biology are presented including the role of nucleotides at early stages (RNA world). On the other hand, hypotheses on the development of early metabolisms, compartments and genetic encoding are also discussed in relation with their role in extant living organisms. The nature of the Last Common Ancestor is also presented as well as hypotheses on the evolution of viruses. The following sections constitute a collection of independent articles providing a general overview of these aspects.  相似文献   

Maintaining the stability of deviated and horizontal wells: Effects of mechanical,chemical and thermal phenomena on well designs     

《Geomechanics and Geoengineering》2013,8(3):167-178

Wellbore instability, particularly in shale formations, is regarded as a major challenge in drilling operations. Many factors, such as rock properties, in-situ stresses, chemical interactions between shale and drilling fluids, and thermal effects, should be considered in well trajectory designs and drilling fluid formulations in order to mitigate wellbore instability-related problems. A comprehensive study of wellbore stability in shale formations that takes into account the three-dimensional earth stresses around the wellbore as well as chemical and thermal effects is presented in this work. The effects of borehole configuration (e.g. inclination and azimuth), rock properties (e.g. strength, Young's modulus, membrane efficiency and permeability), temperature and drilling fluid properties (e.g. mud density and chemical concentrations) on wellbore stability in shale formations have been investigated. Results from this study indicate that for low-permeability shales, chemical interactions between the shale and water-based fluids play an important role. Not only is the activity of the water important but the diffusion of ions is also a significant factor for saline fluids. The cooling of drilling fluids is found to be beneficial in preventing compressive failure. However, decreasing the mud temperature can be detrimental since it reduces the fracturing pressure of the formation, which can result in lost-circulation problems. The magnitude of thermal effects depends on shale properties, earth stresses and wellbore orientation and deviation. Conditions are identified when chemical and thermal effects play a significant role in determining the mud-weight window when designing drilling programmes for horizontal and deviated wells. The results presented in this paper will help in reducing the risks associated with wellbore instability and thereby lowering the overall non-productive times and drilling costs.  相似文献