Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis     

Chen Yueqin  Shao Peng  Wang Ning  Zhou Hui  Qu Lianghu  Linda K. Medlin 《海洋学报(英文版)》2003,22(2):243-253

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.  相似文献   

Composition and distribution of copepods, a major prey of capelin in the central Barents Sea, in moderate and warm years     

Orlova  Emma L.; Boitsov  Vladimir D.; Nesterova  Valentina N.; Ushakov  Nikolai G. 《ICES Journal of Marine Science》2002,59(5):1053-1061

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LabVIEW设计中压力传感器的RBF神经网络温度补偿   总被引：5，自引：0，他引：5  

毕海军  傅圣雪  冯遵成 《中国海洋大学学报(自然科学版)》2004,34(6):1041-1044

在石油平台注水压力监测系统设计中 ,采用LabVIEW虚拟仪器平台 ,嵌入逼近能力强和收敛速度快的RBF神经网络 ,以人工环境实验数据为样本进行训练 ,实现了压力传感器的智能网络温度补偿。结果显示 ,此方法能够在压力、温度变化较大的恶劣环境下 ,获得很高的补偿精度。  相似文献   

Seasonal and annual variations of marine sinking particulate flux during 1993~1996 in the central South China Sea   总被引：1，自引：0，他引：1  

CHEN Ronghu  WIESNER M G  ZHENG Yulong  CHENG Xinrong  JIN Haiyan  ZHAO Qingying  ZHENG Lianfu 《海洋学报(英文版)》2007,26(3):33-43

A total of 67 samples from the upper and lower sediment traps in the central South China Sea were analyzed, which were collected during 1993~1996. It is indicated that the distribution of stable isotope values, surface primary productivity, fluxes of total particulate matter, carbonate, biogenic opal, organic carbon, planktonic foraminiferal species and their total amount exhibit obviously seasonal and annual fluctuations. High values of the fluxes occurred in the prevailing periods of the northeastern and southwestern monsoons, and the low values occurred during the periods between the two monsoons. The fluxes of some planktonic foraminiferal species (Globigerinoides sacculifer, G. ruber, Globigerinita glutinata, Neogloboquadrina dutertrei) and their percentages also exhibit two prominent peaks during the prevailing periods of the northeastern and southwestern monsoons respectively, while those of Globigerina bulloides, Globorotalia menardii and Pulleniatina obliquiloculata only exhibit one peak in the prevailing periods of the northeastern monsoon. In addition, fluxes and percentages of Globigerinoides sacculifer and Globorotalia menardii as well as the fluxes of carbonate and total amount of planktonic foraminifera decrease gradually from 1993 to 1996, and those of Globigerina bulloides, Globigerinita glutinata and biogenic opal increase gradually from 1993 to 1996. The fluxes of carbonate and organic carbon in the upper trap are higher than those in the lower one. The study indicates that the seasonal and annual variations of the sediment fluxes and planktonic foraminiferal species are mainly controlled by the changes of surface primary productivity and hydrological conditions related to the East Asian monsoon. The lower carbonate and organic carbon fluxes in the lower trap are related to the dissolution.  相似文献   

东亚冬夏季风对热带印度洋秋季海温异常的响应   总被引：5，自引：0，他引：5  

李东辉  谭言科  张瑰  叶志敏 《热带海洋学报》2006,25(4):6-13

利用多年的Reynolds月平均海表温度资料和NCEP/NCAR全球大气再分析资料,分析了热带印度洋秋季海表温度距平(SSTA)与后期东亚冬夏季风强度变化的关系。结果表明,热带印度洋秋季SSTA的主要模态是全区一致(USB)型和偶极子(IOD)型,USB型模态主要代表热带印度洋秋季SSTA的长期变化趋势,而IOD型模态主要反映热带印度洋秋季SSTA的年际变化。热带印度洋秋季海温气候变率中既存在着明显的ENSO信号,也有独立于ENSO的变率特征,独立于ENSO的热带印度洋秋季SSTA变化的主要模态仍是USB型和IOD型。前期秋季USB模态与东亚冬季风及东亚副热带夏季风之间为负相关关系;与前期正(负)IOD模态相对应,南海夏季风强度偏弱(强),而东亚副热带夏季风强度偏强(弱)。USB型和IOD型模态对后期东亚冬、夏季风强度变化的影响是独立于ENSO的,但ENSO起到了调节二者相关显著程度的作用。  相似文献   

胶州湾夏季异养浮游细菌的时空变化规律及影响因素   总被引：3，自引：0，他引：3  

白洁  李岿然  张昊飞  张经  魏皓  刘东艳  高冬梅 《海洋科学》2005,29(11):58-62

2001年夏季对胶州湾异养浮游细菌在一个潮周期内的变化规律及影响因素进行了研究。结果表明,胶州湾异养浮游细菌数量的垂直分布特征是表层大于底层,表层平均8.99×109个/L,底层平均5.23×109个/L。胶州湾水体异养浮游细菌日变化幅度在表层水体较为明显,底层相对较小,但其变化规律均为最高值在小潮期而最低值出现在大潮期。浮游动物摄食、浮游植物光合作用产生的溶解有机物及水温和日光中的紫外辐射是影响胶州湾异养浮游细菌昼夜变化的主要因素,水交换是影响其日变化的主要因素。  相似文献   

热带印度洋偶极子发生和演变机制的数值研究   总被引：5，自引：0，他引：5  

李东辉  张铭  张瑰  谭言科 《海洋科学进展》2005,23(2):135-143

对中国科学院大气物理研究所(IAP)大气科学和地球流体力学数值模拟国家重点实验室(LASG)发展的第三代海洋模式(L30T63 OGCM)进行了改进。分析了该模式1959年1月—1998年12月的40a积分结果，以此研究热带印度洋偶极子发生、发展和消亡的物理机制。对数值模拟结果的分析表明，赤道印度洋表面异常东风引起的异常环流结构是偶极子发生、发展的主要动力学原因，其表面异常东风转换为异常西风所引起的异常环流结构调整是偶极子消亡的主要动力学原因；海气界面热通量异常的交换对热带印度洋海表温度距平偶极子模态的形成和演变起着重要的作用；垂直输送作用是热带印度洋次表层海温偶极子模态发生和演变的主要物理机制。  相似文献   

Seasonal population dynamics of Octopus vulgaris in the eastern Mediterranean     

Katsanevakis  Stelios; Verriopoulos  George 《ICES Journal of Marine Science》2006,63(1):151-160

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Aliasing due to sampling of the Adriatic temperature, salinity and density in space     

Zoran Pasari&#x;  Mirko Orli&#x;  Craig M. Lee 《Estuarine, Coastal and Shelf Science》2006,69(3-4):636

High-resolution underway temperature and conductivity measurements collected by R/V Knorr during winter and spring 2003 are used to characterize errors associated with spatial aliasing in the northern and central Adriatic Sea. During winter, 99th percentile temperature, salinity and density errors were 0.62 °C, 0.25 and 0.12 kg/m³ (0.25 °C, 0.10 and 0.05 kg/m³) for sampling at 10 km (5 km) horizontal resolution, respectively. The corresponding values in spring were 1.31 °C, 0.50 and 0.40 kg/m³ (0.93 °C, 0.25 and 0.22 kg/m³) for the 10 km (5 km) sample spacing, respectively. The largest errors were associated with energetic regions over the shallow, western Adriatic, in front of the Po River mouth and off the tip of the Istrian peninsula. The deeper eastern basin exhibited smaller errors. The variability of errors in time and space reflected the variability of small-scale density features, characterized by wavelengths as small as 2 km in winter and 1 km in spring and being more pronounced in the western and northern parts of the Adriatic. As these results indicate that errors associated with undersampling can be considerable, they should be taken into account while planning future CTD measurements in the region.  相似文献   

A General Linear Wave Theory for Water Waves Propagating over Uneven Porous Bottoms   总被引：1，自引：0，他引：1  

锁要红  黄虎 《中国海洋工程》2004,18(1):163-171

Starting from the widespread phenomena of porous bottoms in the near shore region, considering fully the diversity of bottom topography and wave number variation, and including the effect of evanescent modes, a general linear wave theory for water waves propagating over uneven porous bottoms in the near shore region is established by use of Green‘s scond identity. This theory can be reduced to a number of the most typical mild-slope equations curreutly in use and provide a reliable research basis for follow-up development of nonlinear water wave theory involving porous bottoms.  相似文献