Study on Application of Polyenzyme Method to Offal of Harengula zunasi     

邓尚贵  杨萍  夏杏洲 《中国海洋湖沼学报》2003,21(4):312-318

The new polyenzyme method for making gravy from Harengula zunasi offal involves protein enzymolysis with flavorase after proper alkaline and neutral protease levels were established by orthogonal trials to select the best hydrolytic conditions for processing offal with alkaline and neutral protease. The conditions for the polyenzyme method were pH of 7.0, temperature of 50℃ , alkaline and neutral protease concentration of 1.5% respectively, hydrolysis time of 120 min, andflavorase concentration of 2.0% , for 60 min. The new gravy-making technology yields a nutritious and delicious gravy containing 40.3 % of total essential amino acids, with delicious amino acids Glu, Asp, Gly, Ala, Pro and Ser comprising 49.5% , total and amino nitrogen being respectively1.9 and 1.1 g/100 g (amino acid nitrogen being 61.0% of total nitrogen), The polyenzyme method was used to make 14.8% protein gravy from Harengula zunasi offal. In addition, in inorganic elements, the phosphorus content is the highest.  相似文献   

The old open clusters Saurer A, B and C revisited     

Giovanni Carraro  Gustavo Baume 《Monthly notices of the Royal Astronomical Society》2003,346(1):18-26

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Study on application of polyenzyme method to offal of Harengula zunasi     

Deng Shanggui  Yang Ping  Xia Xingzhou 《中国海洋湖沼学报》2003,21(4):312-318

The new polyenzyme method for making gravy fromHarengula zunasi offal involves protein enzymolysis with flavorase after proper alkaline and neutral protease levels were established by orthogonal trials to select the best hydrolytic conditions for processing offal with alkaline and neutral protease. The conditions for the polyenzyme method were pH of 7.0, temperature of 50°C, alkaline and neutral protease concentration of 1.5% respectively, hydrolysis time of 120 min, and flavorase concentration of 2.0%, for 60 min. The new gravy-making technology yields a nutritious and delicious gravy containing 40.3% of total essential amino acids, with delicious amino acids Glu, Asp, Gly, Ala, Pro and Ser comprising 49.5%, total and amino nitrogen being respectively 1.9 and 1.1 g/100 g (amino acid nitrogen being 61.0% of total nitrogen), The polyenzyme method was used to make 14.8% protein gravy fromHarengula zunasi offal. In addition, inorganic elements, the phosphorus content is the highest. This project was aided financially by the Guangdong Science and Technology Office (No. 2KM06002S).  相似文献   

B0850+054: a new gravitational lens system from CLASS     

A. D. Biggs  D. Rusin  I. W. A. Browne  A. G. de Bruyn  N. J. Jackson  L. V. E. Koopmans  J. P. McKean  S. T. Myers  R. D. Blandford  K.-H. Chae  C. D. Fassnacht  M. A. Norbury  T. J. Pearson  P. M. Phillips  A. C. S. Readhead  P. N. Wilkinson 《Monthly notices of the Royal Astronomical Society》2003,338(4):1084-1088

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The Preboreal climate reversal and a subsequent solar‐forced climate shift     

J. van der Plicht  B. van Geel  S. J. P. Bohncke  J. A. A. Bos  M. Blaauw  A. O. M. Speranza  R. Muscheler  S. Bjrck 《第四纪科学杂志》2004,19(3):263-269

Accurate chronologies are essential for linking palaeoclimate archives. Carbon‐14 wiggle‐match dating was used to produce an accurate chronology for part of an early Holocene peat sequence from the Borchert (The Netherlands). Following the Younger Dryas–Preboreal transition, two climatic shifts could be inferred. Around 11 400 cal. yr BP the expansion of birch (Betula) forest was interrupted by a dry continental phase with dominantly open grassland vegetation, coeval with the PBO (Preboreal Oscillation), as observed in the GRIP ice core. At 11 250 cal. yr BP a sudden shift to a humid climate occurred. This second change appears to be contemporaneous with: (i) a sharp increase of atmospheric ¹⁴C; (ii) a temporary decline of atmospheric CO₂; and (iii) an increase in the GRIP ¹⁰Be flux. The close correspondence with excursions of cosmogenic nuclides points to a decline in solar activity, which may have forced the changes in climate and vegetation at around 11 250 cal. yr BP. Copyright © 2004 John Wiley & Sons, Ltd.  相似文献   

固定化鲅鱼乙酰胆碱酯酶的制备及部分性质测定   总被引：3，自引：0，他引：3  

孟范平  何东海  朱小山  马冬冬  杨正先  YANG Zheng-Xian 《中国海洋大学学报(自然科学版)》2005,35(6):1067-1071

酶的固定化是酶传感器制备过程中重要的1个环节.研究采用直接共价法固定鲅鱼乙酰胆碱酯酶（AChE）.制备方法为：0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h.所制备的固定化酶活力回收率较高（96%）,对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%.这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备.  相似文献   

南海及周边部分地区特提斯构造遗迹:问题与思考   总被引：6，自引：1，他引：6  

颜佳新  周蒂 《热带海洋学报》2002,21(2):43-49

对全球二叠纪－三叠纪古气候特征的研究表明，东特提斯地区晚三叠世古气候标志具有带状分布的特征。北巴拉望地块卡拉棉群岛科龙岛的晚三叠世沉积中发育反映较干燥古气候特征的鲕状灰岩，因此在古气候特征方面，北巴拉望地块晚三叠世地层不能与华南和印支地块同期地层对比，而应该与印度地块北缘喜马拉雅地区（向西）和琉球群岛（向东）对比。根据该古气候分析，北巴拉望地块在晚三叠世时应该位于南部亚热带较干燥气候带内，其晚三叠世的古地理位置比印支地块更靠南。对南海及南海周边部分地特提斯构造遗迹的研究，将有助于深入了解南海北部陆缘区相关构造带的性质及正确恢复南海扩张之前该区的地质发展历史。南海北部陆缘区也是研究解决特提斯构造向东延伸问题以及特提斯构造－太平洋构造时空转化问题的关键地区。  相似文献   

添加维生素B_（12）生产菌高密度培养褶皱臂尾轮虫的效果     

于建平  日野明德 《中国海洋大学学报(自然科学版)》1994,(2)

阐述把维生素Ｂ１２生产菌添加到培养水中培养褶皱臂尾轮虫ＢｒａｃｈｉｏｎｕｓＰｌｉｃａｔｉｌｉｓ的买验。共１８株细菌分离于轮虫培养池，其中，有一株产维生素Ｂ１２的假单胞杆菌ＴＰ４对轮虫的生长繁殖有明显的促进作用。把ＴＰ４菌株培养后，加入到２Ｌ的烧杯和５００Ｌ的水槽中培养泰国Ｓ型轮虫时．在９ｄ（天）和６ｄ（天）中，轮虫密度从１２４～１３９和２４２～２８８个体／ｍｌ增殖到４，４１７～５，５４０和１，０１７～１，２５４个体／ｍｌ，分别比对照组增加了４～６及２～３倍。  相似文献   

Metabolites from mangrove endophytic fungus Dothiorella sp.     

XU Qingyan  WANG Jianfeng  HUANG Yaojian  ZHENG Zhonghui  SONG Siyang  ZHANG Yongmin  SU Wenjin 《海洋学报(英文版)》2004,23(3):541-547

Mangroves are special woody plant communities in the intertidal zone of tropical and subtropical coasts. They prove to be a natural microorganisms and new metabolites storage. In the study of mangrove endophytic fungi metabolites, four new compounds, Compounds 1, 2, 3 and 4, as well as a known octaketide, cytosporone B (5), are isolated from an endophytic fungus, Dothiorella sp., HTF3. They all show cytotoxic activities. The elucidation of these structures is mainly based on 1D/2D NMR and ESI-MS spectral analyses.  相似文献   

华贵栉孔扇贝三倍体的研究   总被引：2，自引：1，他引：2  

曾志南  陈木等 《台湾海峡》1995,14(2):155-162

本文报道了用细胞松驰素B、高温和低温休克等方法诱导华贵栉孔扇贝（Chlamys nobilis）三倍体的实验结果。在26.0～28.0℃条件下，选用不同浓度的细胞松驰素B0.1、0.3、0.5、0.7和1.0mg/dm^3，在受精后15min，处理受精卵15min，三倍体诱导率分别为12.5%、37.5%、68.0%、66.6%和81.0%，其中0.5～1.0mg/dm^3浓度的细胞松驰素B对改变倍性最有效。高温休克选用31.0、33.0、35.0和37.0℃处理受精卵，最高三倍体诱导率达46.7%（33.0℃,处理15min）。低温休克选用6.0、8.0、10.0和12.0℃处理受精卵，最高三倍体诱导率为40.7%（10.0℃，处理20min）。实验结果表明，随着处理强度的增大和处理时间的延长，三倍体诱导率增高、但孵化率下降，畸型率上升。用高温休克处理的实验组幼虫生长速度较对照组快。作者认为，在商业性生产中，温度休克是诱导扇贝三倍体的一种简便、有效的方法。文中还就细胞松驰素B与温度休克诱导三倍体的机制进行了讨论。  相似文献