单体褶牡蛎(Crassostrea sp.)的研究     

王昭萍  王如才  徐从先  仇宝志  姜福正  张祝绪 《中国海洋大学学报(自然科学版)》1992,(2)

采用肾上腺素(EPI)和去甲肾上腺素(NE)诱导、颗粒固着基和先固着后脱基三种方法,对褶牡蛎的眼点幼虫进行处理,产生单体蛎苗。EPI和NE诱导的最适浓度为10~(-4)M,最适处理时间为3h,幼虫的不固着变态率分别达47.7％和46.6％。处理时间延长,变态率增加不明显。EPI和NE的诱导效果差别不明显,其诱导作用对稚贝的生长无明显副作用。颗粒固着基以贝壳粉为佳,最适颗粒的规格为幼虫的壳长。幼虫先固着后脱基而形成单体蛎苗,以可弯曲的灰色塑料板效果最好,固着的小贝可通过来回弯曲塑料板而从上面脱落下来,小贝长至1～2cm大小时,脱基最容易。  相似文献   

The hydrogen peroxide impact on larval settlement and metamorphosis of abalone <Emphasis Type="Italic">Haliotis diversicolor supertexta</Emphasis>     

张祥景  杨志会  蔡中华 《中国海洋湖沼学报》2008,26(3):238-241

Abalone Haliotis diversicolor supertexta is an important economic mollusk. The settlement and metamorphosis are two critical stages during its development period, which has direct influence on abalone survival and production. The influence of reactive oxygen species （hydrogen peroxide） on abalone embryo and juvenile development were examined in this study. Larvae of Haliotis diversicolor supertexta were induced to settlement and metamorphose by exposure to seawater supplemented with hydrogen peroxide. They had the best performance at 800 μmol/L. The concentration of 1 000 μmol/L or higher was toxic to the larvae, as the larvae could settle down only at benthic diatom plates without complete metamorphosis. In addition, H2O2 adding time was critical to the larval performance. 24h after two-day post-fertilization was proved to be the optimal adding time. In this paper, two action mechanisms of hydrogen peroxide are discussed： （1） hydrogen peroxide has direct toxicity to ciliated cells, thus cause apoptosis; （2） hydrogen peroxide, as a product from catecholamines＇ autoxidation process in vivo, can reverse this process to produce neuro-transmitters to induce abalone metamorphosis.  相似文献   

Inhibition of Fertilization and Larval Metamorphosis of the Coral Acropora millepora (Ehrenberg, 1834) by Petroleum Products     

Andrew P. Negri  Andrew J. Heyward 《Marine pollution bulletin》2000,41(7-12):420-427

Accidental oil spills from ships or rigs and inputs of effluent such as production formation water (PFW) are key perceived threats to tropical biota from industry activities. Scleractinian corals are an important functional component of tropical reefs and the abundance, diversity and resilience of coral communities can be used as an indicator of ecosystem health. In this paper, we report the effects of petroleum products, including water accommodated fractions (WAF) of crude oil, PFW and dispersant (Corexit 9527), on fertilization and larval metamorphosis of the widespread scleractinian coral, Acropora millepora (Ehrenberg, 1834) in laboratory-based assays. At 20% v/v PFW fertilization was inhibited by 25%. This concentration was equivalent 0.0721 mg l⁻¹ total hydrocarbon (THC). In contrast, larval metamorphosis was more sensitive to this effluent, with 98% metamorphosis inhibited at the same concentration. Crude oil WAF did not inhibit fertilization of gametes until dispersant was introduced. Dispersed oil was slightly more toxic to fertilization than dispersant alone, suggesting toxicity to that event may be additive. The minimum concentration of dispersed oil which inhibited fertilization was 0.0325 mg l⁻¹ THC. Larval metamorphosis was more sensitive than fertilization to crude oil. Although crude oil and dispersant inhibited larval metamorphosis individually, this toxicity was magnified when larvae were exposed to combinations of both. Crude oil inhibited metamorphosis at 0.0824 mg l⁻¹ THC and at 0.0325 mg l⁻¹ THC when dispersed in 10% v/v (dispersant/oil). Management of petroleum-related risks to spawning corals should consider not only the occurrence of the annual coral spawning event, but also the subsequent 1–3-week period during which most larval metamorphosis and recruitment occur.  相似文献   

缅甸中部抹谷早白垩世构造岩浆作用及对特提斯演化的启示     

陈希节  许志琴  Kyaing Sein  孟元库  蔡志慧 《地质学报》2016,90(11):3060-3080

本文系统报道了缅甸抹谷变质带中部Yinmabin花岗闪长岩体及其基性岩墙的主量元素、微量元素及锆石U-Pb年龄和Lu-Hf同位素组成,重点讨论了花岗闪长岩和基性岩墙的岩石成因、物质来源及其地质意义。抹谷Yinmabin花岗闪长岩体主量元素显示富钠(N2O/K2O=1.5~1.75)、准铝(ACNK=0.94~0.97)和钙碱性岩石系列的地球化学特征。样品富集轻稀土(LREE)元素、大离子亲石元素(LILE),相对亏损高场强元素(HFSE)。岩体的主、微量元素地球化学特征均显示其具有活动陆缘I型花岗岩的地球化学属性。抹谷Yinmabin花岗闪长岩的锆石U-Pb年龄为131~135 Ma,其εHf(t)值在-15.66~-2.99之间,平均值为-8.77,单阶段模式年龄(tDM)为795~1569 Ma;而后期侵位的辉绿岩的锆石U-Pb年龄为120~118 Ma,其εHf(t)值在-13.72~+5.82之间,平均值为-2.72,单阶段模式年龄(tDM)为570~1341 Ma,表明这些岩石可能主要来源于古老下地壳部分熔融但同时也受到地幔物质的加入混染。与滇西以及北拉萨岩浆带的Lu-Hf同位素特征十分相近,暗示抹谷-毛淡棉地块早白垩世岩浆岩带与波密-察隅-高黎贡-拉萨岩浆岩带形成环境相似,可能是腾冲岩浆岩带向南延伸的一部分。结合岩石成因、源区性质和区域构造演化,认为抹谷花岗闪长岩体及基性岩墙的形成与中特提斯洋板块的俯冲作用密切相关,同时俯冲-碰撞转换的时限应该在120 Ma左右。这对于研究抹谷地体在中生代期间中特提斯洋俯冲碰撞过程具有重要的地质意义。  相似文献   

Chemical induction of settlement and metamorphosis in two Babylonia (Gastropoda) larvae     

Ke Caihuan  Li Shaojing  Li Fuxue  Zheng Zhon  Liu Weibin 《海洋学报(英文版)》2000,19(1):59-67

INTRODUCTIONStudiesonchemicalinductionofsettlementandmetamorphosisinmarineGastropodalarvaehaveadvancedconsiderablyoverdecades,manyofwhichfocusonHalionsrufescensandPhestillasibogae.AccordingtotheavailabledataItheeffectivefactorswhichcaninduceGastropodalarvaetosettleandmetamorphose,includenatureinducers,neurotransmittersandtheirstructuralanalogs,inorganicion(K )andorganicsolvents.Sincethemechanismofmetamorphosisingastropodlarvaediffersmarkedlyamongvariousspecies,itisnecessarytoexamineandc…  相似文献   

Algal epibiosis on Megabalanus tintinnabulum and its role in segregation of the Balanus amphitrite population     

Ranjith Eswaran  Lidita Khandeparker 《Marine Ecology》2014,35(4):492-505

Ulva sp. and Gracilaria sp. were found colonizing shell surfaces of an acorn barnacle, Megabalanus tintinnabulum. However, this association was not noticed in the case of Balanus amphitrite, which was a co‐inhabitant. Such a difference in intergeneric interactions with the algae was examined in laboratory experiments. For this, the influence that extracts of algae, extracts of algae‐associated bacteria and natural leachants from M. tintinnabulum exerted on cyprid metamorphosis of B. amphitrite was examined. Extracts of algae and associated bacteria showed no effect on the metamorphosis of B. amphitrite. This may be attributed to absence of cue‐specific sugars in the exopolysaccharides and culture supernatants of bacteria. On the other hand, natural leachants of M. tintinnabulum, which showed the presence of N‐acetyl‐d ‐glucosamine, a known algal spore attractant, also inhibited metamorphosis of B. amphitrite. Thus, hosting specific epibionts could have important roles in the segregation of barnacle population.  相似文献   

Larval life span,larval settlement,and early growth of Evechinus chloroticus (Valenciennes)     

M. M. Walker 《新西兰海洋与淡水研究杂志》2013,47(4):393-397

Development, settlement, and subsequent metamorphosis on several substrata were observed in cultures of Evechinus chloroticus larvae. The period from fertilisation to metamorphosis ranged between 30 and 63 days for larvae collected from spawnings induced at 3 different times. Newly settled sea urchins collected from the field grew about 1 mm per month in aquaria. Correlation of the larval life span with the growth rate of newly settled sea urchins suggested that the earliest spawning could occur was mid‐November. This finding corroborates the results of previous histo‐logical studies of the gonads of adult sea urchins in the Hauraki Gulf, New Zealand.  相似文献   

砗蚝(Hippopus hippopus)的人工繁育   总被引：1，自引：0，他引：1  

张跃环  肖述  李军  马海涛  张扬  向志明  喻子牛 《海洋与湖沼》2017,48(5):1030-1035

于2016年4—9月开展了砗蚝(Hippopus hippopus)人工繁育技术研究。采用五羟色胺进行催产、促使配子排放;精卵分别收集,进行异体间受精以避免自交;受精卵经过30h孵化,选出600万D形幼虫进行培育。砗蚝的早期生活史与砗磲(Tridacna spp.)相似,经历前期面盘幼虫、中期面盘幼虫、后期面盘幼虫、足面盘幼虫、单水管稚贝、双水管稚贝、外套膜触手稚贝、幼贝等阶段。与砗磲不同的是,砗蚝怀卵量较少,但卵径较大,D型幼虫也较大,幼虫趋光性更强,壳长2.0mm以后外套膜不伸出壳缘外,幼贝贝壳形态也不同于砗磲。同砗磲幼虫一样,砗蚝幼虫需要构建虫黄藻系统之后,才能出现鳃、次生壳等,从而完成变态成为稚贝。砗蚝幼虫变态率较低,仅为1.4%。中间育成期间,丝状藻和锥形螺是稚贝培育的主要敌害,需及时清理才能确保稚幼贝正常生长发育。经过120d的精心饲育,培养出平均壳长6.3mm幼贝500余个。本研究为进一步开展砗蚝人工繁育、中间育成、增殖放流、资源修复及移植保育提供了参考。  相似文献   

太平洋牡蛎幼体附着变态差异蛋白质组研究          下载免费PDF全文

杨丙晔  濮菲  陈仲巍 《应用海洋学学报》2018,37(3):372-379

太平洋牡蛎(Crassostrea angulata)是中国主要的海洋养殖经济物种之一,具有重要的经济价值和商业价值,附着变态是牡蛎幼体从受精卵发育到成体所需的重要阶段,其分子机制的研究对牡蛎养殖育苗具有重要的指导意义.本研究利用双向电泳技术分离筛选牡蛎幼体附着变态过程中的差异表达的蛋白,进一步利用质谱技术鉴定幼体附着变态过程中发育相关的关键蛋白,共筛选出128个差异蛋白,鉴定并注释了39种蛋白.通过对差异表达蛋白功能分析,筛选获得了与纤毛组建相关的筑丝蛋白和与稳定钙离子胞内浓度相关的钙网蛋白,为揭示牡蛎幼体面盘组织及纤毛的退化消失和钙离子有效诱导幼体附着变态的机制提供分子数据.同时还获得了调节脂肪酸β-氧化、三羧酸循环和糖酵解重要生理功能的关键酶蛋白:乙酰辅酶A脱氢酶、异柠檬酸脱氢酶和3-磷酸甘油醛脱氢酶,以及调节无脊椎动物机体能量存储和释放的酶蛋白:精氨酸激酶和ATP合成酶,进一步阐明了牡蛎幼体在附着变态过程中能量调节的分子机制.  相似文献   

The hydrogen peroxide impact on larval settlement and metamorphosis of abalone Haliotis diversicolor supertexta     

张祥景  杨志会  蔡中华 《中国海洋湖沼学报》2008,26(3)

Abalone Haliotis diversicolor supertexta is an important economic mollusk.The settlement and metamorphosis are two critical stages during its development period,which has direct influence on abalone survival and production.The influence of reactive oxygen species(hydrogen peroxide) on abalone embryo and juvenile development were examined in this study.Larvae of Haliotis diversicolor supertexta were induced to settlement and metamorphose by exposure to seawater supplemented with hydrogen peroxide.They had the best performance at 800 μmol/L.The concentration of 1 000 μmol/L or higher was toxic to the larvae,as the larvae could settle down only at benthic diatom plates without complete metamorphosis.In addition,H2O2 adding time was critical to the larval performance.24h after two-day post-fertilization was proved to be the optimal adding time.In this paper,two action mechanisms of hydrogen peroxide are discussed:(1) hydrogen peroxide has direct toxicity to ciliated cells,thus cause apoptosis;(2) hydrogen peroxide,as a product from catecholamines' autoxidation process in vivo,can reverse this process to produce neuro-transmitters to induce abalone metamorphosis.  相似文献