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11.
Proteomics was used to reveal the differential protein expression profiles of acute responses to copper sulfate exposure in larvae of Artemia sinica.Fourteen differentially displayed protein spots were detected and seven of them were identified.Three spots were up-expressed and identified:actin, heat shock protein 70,and chaperone subunit 1;three down-regulated proteins were identified:arginine kinase,elongation factor-2,and glycine-rich protein;and a newly expressed protein was identified as peroxiredoxin.The study indicates the involvement of all the differentially expressed proteins in the early responses of protein expression,and in the survival of A.sinica in the presence of copper and other heavy metals;the findings improve understanding of the organism’s adaptive responses and resistance.  相似文献   
12.
INTRODUCTIONKnowledgeofgametogenesisinvaluablemarineanimalsisveryimportanttocommercialfish ery.Gametogenesisinbivalveswasextensivelystudiedatdifferentlevels (Dohmen ,1 983 ;DorangeandLePennec ,1 989a,b) .Scallopsandothermarinebivalveshavesimilarreproductivecycle…  相似文献   
13.
Histological characteristics of gametogenesis of two kinds of scallops, gonochoric Chinese scallop, Chlamys farreri and hermaphroditic bay scallop, Argopecten irradians were investigated in this study. Spermatogenesis in C. farreri has different developmental stages: spermatogonia, primary spermatocyte, second spermatocyte, spermatid and spermatozoon. A large number of same developmental stage spermatic cells converge at a definite area of the testis. Premeiotic, previtellogenic and vitellogenic oocytes can be found during oogenesis in C. farreri, where oocyte distribution is obviously irregular. The A. irradians gonad consists of two different parts in one individual: one part functions as testis, the other as ovary. Between these two parts is a special appearance area, where a large number of spermatic cells are bound with two layers of acellular substance with many oocytes in it.  相似文献   
14.
海洋动物多倍体的诱导目前已成为海水养殖中品种改良的重要手段(楼允东,1984)。在美国,太平洋牡蛎和虹鳟的多倍体诱导已大规模应用于生产,并获得良好的经济和社会效益。我国也先后开展了鱼类(桂建芳等,1991;尤锋,1993)、中国对虾(戴继勋等,1993;相建海等,1992)、贝类(喻子牛等,1995)及中华绒螯蟹(陈立侨等,1997)等多种海洋动物多倍体诱导的研究,并取得了一些进展。6-二甲基氨基嘌呤(6-DMAP)由于其低毒、经济和高效,目前已成为海洋动物多倍体诱导中经常使用的诱导剂,但至今,国内外尚无关于6-DMAP在卵子内的残留的报道。在环境保护和生物安全倍受关注的今天,人们非常关心多倍体诱导食物的安全性问题,本文首次利用高压液相色谱技术检测了在中国对虾和中华绒螯蟹多倍体诱导处理后,卵子和幼体内6-DMAP的残留量,并对检测结果进行了分析讨论。  相似文献   
15.
激光扫描共聚焦显微镜是以激光为光源,以照明点和探测点共轭光路为探测系统对自发荧光样品或可被荧光标记的样品进行共焦、实时、真彩色扫描的一种新型光学显微镜,目前在生物学各领域已得到广泛应用。在海洋生物学领域主要用于海洋动物发育生物学、胚胎学、受精细胞学及细胞分化的研究。本文对其原理、操作、优越性及在海洋生物学中的应用等内容进行了综述。1 激光扫描共聚焦显微镜的原理激光扫描共聚焦显微镜是以激光作为光源,通过激光孔及物镜的聚焦作用形成一个光点照射到样品上激发出荧光的一种光学显微镜。物镜的质量决定了共聚焦光学…  相似文献   
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