中华绒螯蟹病原鳗利斯顿氏菌生物学特性   总被引：8，自引：1，他引：8  

陈翠珍  房海  张晓君  葛慕湘  王秀云  靳晓敏 《湖泊科学》2006,18(3):293-298

2004年3月-2005年7月对抚仙湖鱼类资源进行了调查,共采集到鱼类33种,隶属于13科30属,其中土著鱼类14种,特有鱼类4种．渔获物种类主要为:太湖新银鱼、云南倒刺鲃、抚仙鲇、黄颡鱼和鲫等．抚仙湖鱼类群落结构发生了显著变化,鱇浪白鱼等土著鱼类资源严重衰竭．与1995年相比,11种土著鱼类未采到,其他鱼类增加5种．  相似文献   

青藏公路边坡防护试验研究     

陈济丁  何子文  房海  李齐军 《冰川冻土》2004,26(Z1):291-295

The results brought out in the trials of slope protection along Qinghai-Tibet Highway are presented in this paper. The trials were carried out simultaneously at 5 sites in Qinghai-Tibet Plateau from 2000 to 2002. Altitudes at the experimental sites range between 4 240 m and 5 040 m. 4 sites are in permafrost area, and 1 site is in seasonally frozen ground. According to the trials of slope protection, vegetation is preferred to protect slopes along Qinghai-Tibet Highway. Road-GoodR, a chemical stabilizer, is proved as a good material for slope protection, and soil engineering system, combined with vegetative component and grade stabilization structures is proved as the best slope protection measure in these are as. The results showed that high-altitude areas at an altitude lower than 5 040 m, annual average temperatures higher than -5.6 ℃ and annual rainfall more than 262.2 mm, slopes can be protected using vegetative components.Trials for plant species selection proved that cold resistant grasses, Elymus nutans and Elymus sibiricus can be used for vegetation recovery along Qinghai-Tibet Highway. The results demonstrated that high-altitude areas at an altitude lower than 5 040 m, annual average temperatures higher than -5.6 ℃ and annual rainfall more than 262.2 mm, could be replanted. Hydroseeding proved to be a good planting technique, and mulch materials benefited vegetation recovery in such area.The experiment also proved that planting could improve slope stability, protect the ecological environment, and improve the roadside landscape.  相似文献   

牙鲆病原秦皇岛弧菌的血清型及荧光抗体检验     

陈翠珍  房海  张晓君  范莉 《海洋通报》2007,26(1):45-49

用分离于病(死)牙鲆(Paralichthys olivaceus L.)的一种新病原弧菌——秦皇岛弧菌(Vibrio qinhuangdaora sp.nov.)的代表菌株(HQ010712-1株),分别制备全菌(OK)及热处理(121℃作用1h)的菌体(O)免疫原,强化免疫接种家兔制备相应抗血清,对供试12株秦皇岛弧菌进行了血清型检定,结果表明均存在同种的K抗原和同种的O抗原(血清同源),其中O抗原具有强免疫原性、K抗原的免疫原性弱但具有强O凝集抑制作用;以相应OK抗血清为第一抗体,以标准的羊抗兔IgG荧光抗体为第二抗体,对12株秦皇岛弧菌的纯培养物及用代表菌株(HQ010712-1)人工感染病死牙鲆的肝组织进行了间接荧光抗体染色,结果显示了特异的荧光反应。  相似文献   

凡纳滨对虾(Litopenaeus vannamei)病原副溶血弧菌(Vibrio parahaemolyticus)的表型及分子特征   总被引：5，自引：0，他引：5       下载免费PDF全文

张晓君  陈翠珍  阎斌伦  房海  秦国民  徐静 《海洋与湖沼》2009,40(5):654-662

采用常规的形态及生理生化特性检验及分子生物学等方法,对引起凡纳滨对虾幼虾发生毁灭性死亡的病原菌进行了致病性、表型生物学及分子生物学的系统研究。结果表明,分离菌为弧菌属(Vibrio Pacini 1954)的副溶血弧菌(Vibrio parahaemolyticus),对凡纳滨对虾、中国对虾及日本对虾仔虾均有强致病性。分离鉴定的4株副溶血弧菌具有淀粉酶、明胶酶、卵磷脂酶活性,但不具有蛋白酶、脂肪酶活性,KP溶血均呈阴性,且均具有K抗原;代表菌株(JGB080708-1株)的16S rRNA基因序列(长度为1456bp,GenBank登录号为GQ205448)和gyrB基因序列(长度为1195bp,GenBank登录号为GQ205453)与副溶血弧菌的两种基因序列相似性均可达98%以上。病原菌的耐药性检测结果显示,对供试49种抗菌药物中的林可霉素等6种药物耐药。  相似文献