基于转录组测序对两种氮素营养条件下多形微眼藻氮代谢途径的解析     

李迎  米铁柱  乔玲  甄毓 《海洋与湖沼》2019,50(6):1241-1251

探究微藻的氮代谢通路对了解其对不同氮源利用的分子机制具有重要意义。本研究利用Illumina高通量测序技术对两种氮素营养条件下(硝酸氮和尿素)多形微眼藻的转录组进行分析,通过基因功能注释及数字基因表达谱分析,研究了多形微眼藻细胞内氮代谢的调控机制。结果检测出15种参与氮代谢的酶,对应76个编码基因,构建了多形微眼藻的氮代谢通路图。其中10个酶编码基因在两种不同氮素营养条件下存在差异表达,最显著的是谷氨酸合成酶、谷氨酸脱氢酶和谷氨酰胺合成酶相关基因。有机氮源(尿素)实验组中,多形微眼藻细胞内的硝酸盐还原酶、亚硝酸盐还原酶等基因的差异表达明显高于无机氮源(硝酸钠)实验组,表明当环境中的氮源为尿素时,会对多形微眼藻细胞内硝酸盐的转化和利用有一定影响。本研究初步阐述了硝酸盐、尿素的吸收转运对多形微眼藻细胞内氮代谢的影响机制,可为硅藻在不同氮素营养条件下的吸收利用机制及氮代谢响应研究提供依据。  相似文献   

平均海平面模型的确立方法研究     

孙啸  周兴华  孙学华  孙翠羽 《海岸工程》2011,30(2):30-37

针对平均海平面的定义,分别介绍了利用验潮站资料和卫星高度计资料确立平均海平面的方法原理,并对2种方法的特性及存在的问题进行了比较阐述,确定了以沿岸长期验潮站为控制条件,对卫星测高数据确定的高分辨率高精度的平均海平面网格模型进行改正,最终构建平均海平面与国家大地坐标系关系的研究方法.  相似文献   

曹妃甸填海工程阻断浅滩潮道初期老龙沟深槽的地形变化     

尹延鸿  褚宏宪  李绍全  胡刚  毕世普  袁红明 《海洋地质前沿》2011,(5):1-6

通过研究1992、1997、2006年的测深资料,对比分析了横切老龙沟深槽口门处的地形横剖面和大致沿老龙沟深槽轴线的地形纵剖面,可知在此期间深槽内的水深有变浅和淤积的趋势.总体看来,老龙沟深槽的淤积厚度在0.61～4.8 m之间,并且老龙沟深槽轴线也有自东向西迁移的趋势.自2004年曹妃甸填海工程的通岛公路阻断浅滩潮道...  相似文献   

单点高频地波雷达资料估算潮能耗散的方法   总被引：1，自引：0，他引：1  

毛华斌  陈举  邱春华  练树民 《热带海洋学报》2011,(6)

使用嵊泗站所布设的地波雷达观测获取的径向流数据,以及嵊泗、芦潮港、岱山3个潮汐观测站水位资料,采用两点近似投影方法反演流场全矢量流速,并用T-Tide程序计算调和常数,分别计算O1、K1、M2、S2各分潮流速场及迟角场,并计算各点上的潮能通量及潮能耗散,得到嵊泗岛以西杭州湾口区域潮能耗散同地形存在良好对应关系,充分证明了采用地波雷达观测数据进行潮能耗散计算这一方法的可行性,供相关工作者作进一步研究和讨论.  相似文献   

吕宋海峡M2内潮生成与传播数值模拟研究     

李欢  陈学恩  宋丹 《中国海洋大学学报(自然科学版)》2011,41(1)

本文在z坐标海洋数值模式HAMSOM中引入了内潮黏性项(Interhal-tide viscosity term),将之运用到吕宋海峡M2内潮的生成与传播过程的数值模拟研究.研究结果表明:(1)在250 m以浅,吕宋海峡产生的M2内潮振幅于温跃层处最大,岛坡附近的内潮明显强于别处,且最大振幅可达到40 m左右;(2)M2内潮的生成源主要集中在伊特巴亚岛西北、巴丹岛西南以及巴布延群岛西北的岛坡;(3)海峡产生的M2内潮向东西2个方向传播.巴丹岛以西的西向能量在吕宋海沟斜向下传播,在到达恒春海脊附近发生反射返回海面,到达海面后再次反射回海底,在此过程中,有高模态的内潮被激发,不同模态间有相消干涉的现象产生.西传的内潮能量分为2支进入南海,产生于巴布延群岛西北的能量分支直接向西南折转进入南海海盆,而产生于伊特巴亚岛和巴丹岛岛坡附近的主要能量则以束状向南海陆架传播,在到达118°E后部分能量折向西南的海盆,其余的能量则沿西北方向传入中国近岸,陆架陆坡地形起着重要的耗散作用.伊特巴亚岛西北有最大的能量产生,向东北传入太平洋.在122°E以东,能量主要以束状向东南传入太平洋.  相似文献   

钱塘江源头之争史来狼烟四起似终有定论钱塘江大潮观潮历来人流如潮似成就景观--钱塘江声名耀升如潮涌一浪高一浪     

《中国地名》2011,(10):41-41

钱塘江是浙江省第一大河,发源于安徽省黄山,流经安徽、浙江两省,古名“浙江”,亦名“折江”或“之江”,其名最早见于《山海经》。钱塘江是越文化的主要发源地之一。河流全长605千米,流域面积5．56万平方千米,年均流量442．5亿立方米,河口潮汐水力资源理论蕴藏量为472万千瓦特。新安江与兰江是钱塘江的源头,于上海市南汇区和宁波市、舟山市嵊泗县之间注入东海,其中杭州附近河段,称为“之江”或“罗刹江”。钱塘江潮被誉为“天下第一潮”。  相似文献   

黄海在有无潮作用下对“布拉万”不同响应的数值模拟研究   总被引：2，自引：0，他引：2  

孟庆军  李培良 《海洋与湖沼》2015,46(6):1241-1254

黄海是典型的强潮驱动的陆架浅海。为了研究黄海对台风的响应特点,本文利用区域海洋模式(Regional Ocean Modeling Systems,ROMS)分别模拟了在有潮和无潮作用下黄海对台风"布拉万"的响应过程。结果表明,不管潮存在与否,"布拉万"经过黄海后都引起了海表面降温和流速的近惯性振荡响应,这种响应主要分布于黄海中部较深区域,带通滤波提取的近惯性流速具有垂向第一模态特征。同时,研究发现强背景潮流能显著地影响黄海对"布拉万"的响应过程。主要结论如下:一方面,由于潮的存在,近岸垂向混合均匀的较暖水体与远岸较冷水体之间会形成潮混合温度锋面,"布拉万"过后,暖水发生了明显的离岸扩张,尽管路径右侧的混合层降温更显著,但是左侧即黄海西岸的暖水扩张更明显;另一方面,潮的存在减弱了布拉万产生的近惯性振荡响应,半日潮流在黄海仍然占据主导地位。在混合层中潮流的作用减弱了"布拉万"产生的近惯性能量,但也使其更易穿过跃层传入黄海内部。  相似文献   

应用实时定量PCR技术检测秦皇岛近岸海域褐潮原因种——抑食金球藻(Aureococcus anophagefferens)     

GUO Hao  LIU Yongjian  ZHANG Qi  YUAN Xiutang  ZHANG Weiwei  ZHANG Zhifeng 《海洋学报(英文版)》2015,34(2):132-136

Aureococcus anophagefferens, a small pelagophyte algae, has caused brown tide blooms in coastal waters of Qinhuangdao in recent years, presenting significant negative impacts on the shellfish mariculture industry. Under standard light microscopy, it is visually indistinguishable from other small algae in field samples due to its extremely small size. In this study, quantitative polymerase chain reaction(q PCR) based on 18 S r DNA sequences was developed and used to detect and enumerate A. anophagefferens. A linear regression(R2 = 0.91) was generated based on cycle thresholds value(Ct) versus known concentrations of A. anophagefferens. Twenty-two field samples collected in coastal waters of Qinhuangdao were subjected to DNA extraction and then analyzed using q PCR. Results showed that A. anophagefferens had a wide distribution in coastal waters along Qinhuangdao. Elevated A. anophagefferens abundance, category 3 brown tide blooms(200 000 cells/m L) occurred at Dongshan Beach and Tiger-stone Beach in August in 2013. In shellfish mariculture areas along coastal waters of Qinhuangdao, 4 stations had category 3 blooms, and 6 stations had category 2 blooms(35 000–200 000 cells/m L) in August and all stations had category 1 blooms(0 to ≤35 000 cells/m L) in October. Quantitative PCR allows for detection of A. anophagefferens cells at low levels in filed samples, which is essential to effective management and prediction of brown tide blooms.  相似文献   

2008年中国沿岸冬季寒潮激发陆架波的小波分析   总被引：1，自引：0，他引：1  

李君益  郑全安  胡建宇  范振华  朱佳  陈涛  朱本璐  徐莹 《海洋学报(英文版)》2015,34(11):22-31

This study applies the wavelet analysis to the tidal gauge records, alongshore winds, atmospheric temperature and pressure along the China coast in winter 2008. The analysis results show three events of sea level oscillations(SLOs) on the shelf induced by winter storms. The first event occurred from January 9 to 21. The SLO periods were double-peaked at 1.6–5.3 and 7.0–16.0 d with the power densities of 0.04–0.05 and 0.10–0.15 m2·d, respectively.The second event occurred from February 5 to 18. The SLO period was single-peaked at 2.3–3.5 d with power density of 0.03–0.04 m2·d. The third event occurred from February 20 to March 8. The SLO periods were doublepeaked at 1.5–4.3 and 6.1–8.2 d with the power densities of 0.08–0.11 and 0.02–0.08 m2·d, respectively. The SLOs propagated along the coast from Zhejiang in north to Guangdong in south. The phase speeds ranged about 9–29m/s from Kanmen to Pingtan, 5–11 m/s from Xiamen to Huizhou and 11–22 m/s from Huizhou to Shuidong. The dispersion relation of the SLOs shows their nature of coastal-trapped wave.  相似文献   

吕宋海峡M₂内潮的三维数值模拟     

李秉天  曹安州  吕咸青 《海洋学报(英文版)》2015,34(11):55-62

A three-dimensional isopycnic-coordinate internal tidal model is employed to investigate the generation,propagation, vertical structure and energy conversion of M2 internal tides in the Luzon Strait(LS) with mooring observations. Simulated results, especially the tidal current amplitudes, agree well with observations,demonstrating the reasonability and accuracy of the model. Results indicate that M2 internal tides mainly propagate into three directions horizontally, i.e., eastward towards the western Pacific Ocean, westward towards the Dongsha Island and southwestward towards the South China Sea Basin. In the horizontal direction, tidal current amplitudes decrease as distance increases away from the LS; in the vertical direction, they show an obvious decreasing tendency with depth. Between the double ridges of the LS, a clockwise gyre of M2 baroclinic energy flux appears, which is caused by reflections of M2 internal tides at supercritical topographies, and resonance of M2 internal tides happens along 19.5° and 21.5°N due to the heights and separation distance of the double ridges. The total energy conversion in the LS is about 14.20 GW.  相似文献