Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis     

Chen Yueqin  Shao Peng  Wang Ning  Zhou Hui  Qu Lianghu  Linda K. Medlin 《海洋学报(英文版)》2003,22(2):243-253

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.  相似文献   

似海底反射的地震速度和AVO效应     

JoseM.Carcione UmbertaTinivella 《海洋地质》2002,(2):23-40,75

我们将浓度和压力作为一个函数得到冰和含气体水合物的地震波速度。同以往以简单的慢度和平均模量及双相模型为基础的理论不同，我们使用了Biot类型的三相理论，这一理论考虑了二种固体（颗粒和冰或笼形物）和一种液体（水）的存在，以及一个含气和水的多孔隙基质。对于固体的Berea砂岩，低于0℃时的纵波理论估计值过低，在含有冰－颗粒相互作用和颗粒胶结时与实验数据产生了好的吻合。严格地讲，水的比例与温度紧密相关。假定已知孔隙的平均半径和标准偏差，那么在一给定温度下的波速拟合就能对整个温度范围的速度进行预测。通过一个粘弹性的单相结构模型来计算反射系数。本文的分析是针对游离气带的顶部（位于含气体水合物的沉积层下）和底部（上履于饱含水的沉积层）进行的。如果似海底反射仅仅是由于胶结气体水合物与含游离气沉积层的分界面造成，我们可以得出结论：（1）在一定的气体饱和度下，很难估算低浓度情况下气体水合物数量。然而，水合物的高、低浓度还是可以区分的，因为它们分别呈现正和负的异常；（2）游离气和饱和度可以从反射振幅中而不是从异常类型中获取；（3）P波转化为S波的反射系数是指示高含量的气体水合物和游离气一个较好指示。另一方面，对于未胶结的沉积物，振幅随偏移距变化曲线总是为正。  相似文献   

“大洋矿产国际会议——天然气水合物专题”论文摘要——4、7100年前最大洪泛期对黑海天然气水合物储集体影响的模拟     

J.Poort  杨木壮 《海洋地质》2002,(3):30-31

  相似文献   

The expression of CYP1A,vitellogenin and zona radiata proteins in Atlantic salmon (Salmo salar) after oral dosing with two commercial PBDE flame retardant mixtures: absence of short-term responses     

Boon JP  van Zanden JJ  Lewis WE  Zegers BN  Goksøyr A  Arukwe A 《Marine environmental research》2002,54(3-5):719-724

The short-term effects of the commercial PBDE flame retardant mixtures Penta-BDE and cta-BDE on the expression of cytochrome P450 1A (CYP1A), vitellogenin (Vtg) and zona radiata proteins (Zrp) were investigated in juvenile salmon (Salmo salar). For this purpose, groups of fish were dosed twice (oral intake at days I and 4) with 10 and 50 mg/kg body weight of both commercial mixtures. The fishes were sacrificed at day 7 (n = 5 for each group) and 14 (n = 6 for each group), and blood, liver, fillet, and brain were collected. Blanks and positive controls were also part of the experiment. The expressions of Vtg, Zrp, and CYPIA were measured with several techniques (EROD, ELISA, Western, Northern and Slot Blot). The values in the groups of fish treated with Penta-BDE or Octa-BDE did not significantly differ from the reference group for any of the parameters tested. In contrast, the positive control groups treated with estradiol-17beta for Vtg and Zrp expression, and beta-naphthoflavone for CYP1A expression did show a significant response, indicating the potential sensitivity of the fishes for the parameters measured. Since the results of the chemical analyses showed concentrations of a number of PBDE congeners in liver, fillet, and brain that were about three orders of magnitude above those of fish from the North Sea, it is concluded that the short-term toxicity of both commercial PBDE mixtures for these endpoints was low.  相似文献   

An aromatase inhibitor and tamoxifen decrease plasma levels of o,p'-DDT and its metabolites in Nile tilapia (Oreochromis niloticus)     

Leaños-Castañeda O  Gold-Bouchot G  Van Der Kraak G  Lister A  Ceja-Moreno V  Simá-Alvarez R 《Marine environmental research》2004,58(2-5):337-342

The objective of this study was to determine if tamoxifen or an aromatase inhibitor (4-hydroxyandrostenedione; 4-OHA) affected plasma concentrations of o,p' -DDT and its metabolites, o,p'-DDD and o,p'-DDE, in mature tilapia. Male and female tilapia were injected 6 times intraperitoneally with o,p'-DDT (40 mg/kg) alone or in combination with 4-OHA (2 mg/kg) over a 12 day period. An additional group of male fish was injected with tamoxifen (5 mg/kg) plus o,p'-DDT. At the end of the treatment period, plasma samples were extracted and analyzed by GC/ECD. Females injected only with o,p'-DDT had significantly higher levels of o,p'-DDT compared with males. Interestingly, females and males treated concomitantly with o,p'-DDT and 4-OHA or tamoxifen had significantly lower concentrations of plasma o,p'-DDT (about 50%) compared with fish treated with only o,p'-DDT. These initial results suggest that an interaction between endocrine-active compounds occurs in vivo in tilapia and may involve alterations in metabolism of o,p'-DDT.  相似文献   

两株琼胶酶高产细菌的筛选和鉴定   总被引：4，自引：1，他引：4  

杜宗军  王鹏  李筠  P.A.W.Robertson  B.Austin 《海洋科学》2002,26(3):1-4

从海洋环境中筛选得到两株琼胶酶的高产菌株，通过形态观察和生理生化反应，确定它们属于弧菌属，通过BIOLOG细菌鉴定系统鉴定，并同弧菌属标准菌株分析比较，确定上方宝剑两株菌都是塔式弧菌（Vibrio tubiashii)，这两株菌在碳源利用方面存在差别。  相似文献   

Esterases as biomarkers in Nereis (Hediste) diversicolor exposed to temephos and Bacillus thuringiensis var. israelensis used for mosquito control in coastal wetlands of Morbihan (Brittany,France)     

Fourcy D  Jumel A  Heydorff M  Lagadic L 《Marine environmental research》2002,54(3-5):755-759

Since 1998, a biomonitoring programme has been implemented to assess the potential impact of chemical mosquito control on macroinvertebrates of the coastal wetlands of Morbihan (Brittany, France). Acetylcholinesterase and carboxylesterases were used as biomarkers to assess the effects of Abate 500e (a.i. temephos) and Vectobac 12 AS (a.i. endotoxins of Bacillus thuringiensis var. israelensis, Bti) in Nereis (Hediste) diversicolor. Esterase inhibition revealed a marked impact of temephos, suggesting preferential contamination of the worms through the food. In Bti-exposed N. diversicolor, random variations of esterase activities were observed, that could not be attributed to the larvicide. However, esterases only reflected indirect physiological effects of Bti, and further investigations are needed to identify biomarkers more specific of Bti endotoxins.  相似文献   

俄罗斯沿岸更新世盆地类型     

A A Cвиотч  朱佛宏 《海洋地质前沿》2004,20(10):30-31

俄罗斯沿岸地区受到分别属于大西洋、北冰洋和太平洋的14个海的海水冲刷，海岸线的总长度超过60000km。在更新世，这些盆地经历过不止一次的幅度不同的海平面的升降作用。在海退时期，广阔的陆架地区变干了，而在海侵时期，沿岸平原和低山岸区被海水淹没。在东北  相似文献   

Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis     

Jonsson H  Sandnes KV  Schiedek D  Schneider R  Grøsvik BE  Goksøyr A 《Marine environmental research》2004,58(2-5):655-658

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.  相似文献   

烟台海胆体内的纤毛虫和太平洋海胆体内纤毛虫的地理分布     

A.斯特列科夫 《海洋与湖沼》1959,2(1):61-67

1957年夏,中苏海洋动物综合考察队在黄海沿岸烟台地区工作时,参加考察队的阿·阿·斯特列科夫教授采集了海胆肠内的纤毛虫,并以此作为这篇文章的材料。在本文内将提出所见到的纤毛虫名称,并分析它们在太平洋地理分布的情况。  相似文献