卫矛醇对C6胶质瘤细胞体外抗癌活性及机制探讨          下载免费PDF全文

张玉岭  张涛  陈宝贵 《应用海洋学学报》2024,40(2):168

目的:研究卫矛醇对C6胶质瘤细胞株的体外增殖及凋亡的影响。方法：将C6大鼠胶质瘤细胞随机分为对照组、卫矛醇干预组、卫矛醇+雷帕霉素组、卫矛醇+3-MA组,各组予不同药物孵育,采用MTT法检测卫矛醇对C6细胞的体外增殖抑制率,筛选出抑制率相对较高且生长状态良好的细胞;应用Hoechest 33342染色透射电镜观察细胞凋亡亚显微结构的改变,采用免疫荧光和Western Blot技术检测与凋亡和自噬相关蛋白的表达水平。结果:卫矛醇对C6胶质瘤细胞增殖有明显的抑制作用,并呈现出良好的浓度-效应相关性,与对照组比较,随着浓度的增加,细胞的增殖抑制率上升(P＜0.01);Hoechst染色结果显示,与对照组相比,共同孵育卫矛醇24 h后的C6细胞出现明显的核固缩现象,证明有大量的细胞出现凋亡;Western Blot结果显示,与对照组相比,促凋亡基因Bax表达上调,抗凋亡基因Bcl-2表达下调。结论:卫矛醇能够抑制C6细胞的增殖,并诱导其凋亡,其作用机制可能与上调Bax、下调Bcl-2有关。  相似文献   

北大别饶拔寨石榴辉石岩P-T-t轨迹及其构造意义     

闫方超  刘庆  马雪盈  何苗 《岩石学报》2021,37(8):2579-2598

P-T-t轨迹作为变质岩的重要研究方法之一，对揭示岩石的构造演化过程具有重要意义。北大别饶拔寨镁铁-超镁铁岩形成的构造环境和就位过程长期以来尚存争议。本文通过岩相学观察、矿物化学研究和温压计计算，揭示出饶拔寨石榴辉石岩经历了四个变质演化阶段：1）超高压变质阶段（M1）：主要根据石榴子石中金红石的出溶，单斜辉石中石英的出溶和磷灰石中不透明矿物的出溶，结合前人研究，认为饶拔寨石榴辉石岩经历过超高压变质阶段（≥2.5GPa）；2）高压麻粒岩相阶段（M2）：矿物组合为石榴子石（变斑晶）和单斜辉石（基质）+斜长石（基质），记录的温压条件为T=648~700℃，P=1.47~1.94GPa；3）中压麻粒岩相阶段（M3）：以石榴子石外围发育的主要由斜方辉石+斜长石组成的内圈"白眼圈"为特征，形成的温压条件为T=781~796℃，P=0.92~0.98GPa；4）角闪岩相阶段（M4）：以石榴子石变斑晶周围发育的外圈"白眼圈"为特征，其矿物组合为角闪石+斜长石的后成合晶，形成的温压条件为T=663~685℃，P=0.50~0.58GPa。石榴辉石岩的锆石SIMS U-Pb定年得到了3组不同的交点年龄，分别为208.1~202.1Ma、227.6Ma和817.7Ma。根据锆石包裹体中角闪石+斜长石的组合，推断208.1~202.1Ma代表了角闪岩相变质年龄的下限，227.6Ma则是高压麻粒岩相的下限年龄，而新元古代年龄（817.7Ma）与区域上的变质岩原岩年龄一致，可能代表了其原岩年龄。结合前人研究，饶拔寨石榴辉石岩记录了顺时针的P-T-t轨迹，揭示了板片俯冲（超高压变质）-碰撞-折返（降压升温过程，~227Ma）-抬升（降压降温过程，208~202Ma）的完整过程。  相似文献   

内蒙古中西部宝音图群石榴角闪岩的地球化学、变质作用及年代学特征     

姜灵  张晋瑞  唐爽  魏春景  初航 《岩石学报》2021,37(12):3759-3780

宝音图群分布于内蒙古中西部狼山、图古日格和达茂旗一带，主要由云母片岩、石英片岩、石英岩、角闪岩和大理岩组成。在狼山格尔敖包沟和图古日格西南地区发育十分典型的石榴角闪岩，均以似层状或透镜状产自云母片岩中，但两地具有不同的矿物组合，前者主要以石榴石+角闪石+斜长石+含钛矿物（金红石和钛铁矿）为特征，后者主要以石榴石+角闪石+绿帘石+含钛矿物（金红石和钛铁矿）为特征。地球化学研究显示两个地区的石榴角闪岩原岩具有相似的化学组成，均为亚碱性玄武岩的拉斑系列。宝音图群的石榴角闪岩具有右倾的稀土配分模式，REE总量为83.31×10^-6~125.9×10^-6，（La/Yb）_N比值为2.17~6.48，δEu=0.87~0.98。Ta、Nb、Ti没有明显的负异常，其配分型式类似E-MORB特点。微量元素构造判别图解表明这些石榴角闪岩的原岩产于板内拉张环境中。本文通过模拟格尔敖包沟样品LS01和图古日格西南地区样品LS35的P-T视剖面图研究其变质作用及矿物演化过程，结果显示这两个样品均经历了早期进变质，峰期以及峰后近等温减压的顺时针型P-T轨迹。样品LS01的峰期温压条件为~11kbar/~735℃，模拟得到的峰期矿物组合可能为石榴石+角闪石+斜长石+黑云母+透辉石+金红石+熔体，P-M（H₂O）视剖面图显示，由于峰后演化过程中存在饱和流体渗透，导致峰期透辉石无法保留。样品LS35的峰期温度压力条件为~8kbar/675℃，其峰期组合为石榴石+角闪石+绿帘石+金红石。两个地区的石榴角闪岩的温度压力条件明显不同，推测宝音图群中呈透镜体或似层状产出的变质基性岩，存在着递增型的变质作用。锆石LA-ICP-MS U-Pb年代学的研究结果显示石榴角闪岩样品LS01的变质锆石年龄为394±8Ma。由此推测这些石榴角闪岩的原岩可能形成于华北北缘在新元古代发育的裂陷盆地，在泥盆纪中期，宝音图群作为华北克拉通的一部分，被卷入到与古亚洲洋闭合有关的造山过程，发育中压型递增变质作用。  相似文献   

水生观赏植物在湿地建设中的应用   总被引：1，自引：0，他引：1  

王正超  文剑平  刘彩霞 《湿地科学与管理》2006,2(2):49-50

水生观赏植物的利用是湿地恢复工作的重要内容之一,直接关系到湿地景观整体效果。在湿地的池边水畔、岩石缝隙、水面等不同的环境中种植各种水生观赏植物能给人一种自然美的感觉。水生观赏植物的姿态、色彩及清香,加强了水体的空间感和美感,并具有净化水体的生态作用。如北京翠  相似文献   

湖南骑田岭花岗岩及其暗色微粒包体的地球化学与壳幔岩浆的混合作用   总被引：12，自引：2，他引：12  

付建明  谢才富  彭松柏  杨哓君  梅玉萍 《地球学报》2006,27(6):557-569

骑田岭岩体是南岭地区燕山早期具幔源组分贡献的花岗岩典型代表。其主体岩性为角闪黑云二长花岗岩和(角闪石)黑云正长花岗岩,其中发育暗色微粒包体和由暗色矿物组成的团块或条带。暗色微粒包体具有岩浆混合的大部分岩相学证据。如包体的浑圆状外形、塑性形变、冷凝边、斜长石An的“双峰式”分布、似环斑长石、针状磷灰石等。包体属于准铝质(A/KNC=0.72～1.00,平均0.85)钾玄岩系列岩石,寄主岩石为准铝或弱过铝质(A/KNC=0.89～1.06,平均0.97)高钾钙碱性系列的岩石。二者在主量和微量元素上表现出岩浆混合成因的演化趋势。包体与寄主岩石的同位素组成具趋同性,它们的ISr和εNd(t)值分别为0.71041～0.71263、-6.9～-5.3和0.70854～0.71416、-9.2～-5.1,均表现出壳幔混源花岗岩类岩石的特点。包体K-Ar年龄为152Ma,与其寄主岩石的形成年龄(155～161Ma)接近,显示岩浆混合作用发生的时间大致为晚侏罗世早期。对包体及其寄主岩石产出的构造背景和地球化学特征的综合分析表明,该岩体中的暗色微粒包体是在伸展作用的大地构造背景下,上涌的幔源基性岩浆及其诱发的长英质酸性岩浆混合作用的产物。  相似文献   

“第34届国际水文地质大会”即将在京召开     

钟立勋 《中国地质灾害与防治学报》2006,17(3):79-79

水文地质学作为与国民经济发展密切相关的应用学科，在世界各国水资源调查、保护和管理方面发挥着重要的支撑作用。2006年是国际水文地质学家协会（IAH）成立五十周年。因此，2006年10月09日-2006年10月13日国际水文地质学家协会和中国国土资源部将在北京市联合举办“第34届国际水文地质大会”。这将是继1988年中国在桂林市成功举办“第21届国际水文地质大会”之后，再次在中国举办国际水文地质大会。  相似文献   

Possibility of degradation of phenylarsonic acid in the presence of ferrihydrite     

Yoko Shimamoto Yoshio Takahashi 《中国地球化学学报》2006,25(B08):114-114

Although inorganic species are predominant in natural systems, but there are many kinds of organoarsenic species such as methylated and phenylated arsenic compounds. Phenylarsonic acid （PA） is a degradation product of organoarsenics used for chemical warfare agents, which has been detected in well water at the disposal site of the agents in Japan. There are few reports studying behavior of PA in soil. In this study, PA was adsorbed onto ferrihydrite and its chemical forms were determined using high performance liquid chromatography connected to inductivity-coupled plasma mass spectrometry （HPLC-ICP-MS）. 100 mg/kg of PA was mixed with 0.03 g of 2-line ferrihydrite. For each suspension, pH was adjusted by HNO3 or NaOH. Each sample was incubated for more than 19 hours and the final pH was measured. After filtration, the chemical form of arsenic in the filtrate was measured using HPLC-ICP-MS. In addition, ferrihydrite separated by filtration was dissolved by 3 ml of 0.5 M HCI and the arsenic species in the solution was detected by HPLC-ICP-MS （column： Tosoh TSKgel SuperlC-AP, eluent： 0.01 M HNO3）. It was verified that PA is not degraded by heating in 0.5 M HCl solution. At pH 3.1, any arsenic compounds were not detected from the solution, because almost all arsenic species were adsorbed onto ferrihydrite at lower pH. At pH= 12, however, 7%-10% of inorganic arsenic was detected in the solution. In solid phase, there are some problems to determine the precise ratio of inorganic and organic species. When the solution includes Fe ion at 0.01 M level, the retention time of arsenic species drifted compared to those in standard solution, which makes it difficult to determine precisely the arsenic species adsorbed on ferrihydrite. Therefore, more study is needed to determine the ratio of inorganic and organic species in the system.  相似文献   

Overcoming flood and desiccation made by implementing artificial recharge toward absorption reservoir     

Teddy W. Sudinda Teddy 《中国地球化学学报》2006,25(B08):156-157

Flood and desiccation are perceived as two most critical and influential disasters which contradict between causes and consequences. Flood occurs when the water surface could no longer flow the whole water flow, thus, the water flooded. Contradicted to that, desiccation occurs when the water flow contains a low volume of water deposit, thus, the water requirement exceeds the available potential. That condition was caused by land utilization as the consequences due to the increased land requirement for housing or industrial needs. An attempt to overcome the flood, nowadays, is implemented mostly in a structural way, through building canals, implementing rivers normalization, building gateways or building flood control pump which are more directed toward the flood direction in order to increase the surface flow in an immediate maner to the sea. However, the effort to overcome the flood its self, could be more effective if followed by an effort to increase the soil ability to absorb natural recharge or artificial recharge or by refilling the water into the earth surface. Absorption reservoir used as one of technology alternatives （artificial recharge） could also be used to support the attempt to overcome flood and desiccation. Absorption reservoir is a dam which was designed according to the basic principles, such as the bottom surface of the reservoir that has a high permeability surface; the surface of the reservoir water is higher （higher aquifer） along with a high permeability, considering the availability of water source that has been absorbed and its quality; considering the aquifer category on the water absorption dam;  相似文献   

Early diagenesis of nurtrients （C, N, P and Si） stored in sediments of two reservoirs in southwestern China     

Yuchun WANG Jun ZHU Congqiang LIU 《中国地球化学学报》2006,25(B08):161-162

Damming is a common anthropogenic intervention along the course of rivers, which is defined as ＂artificial-lake effect＂, both in China and across the world. Today as many as 48000 dams and/or reservoirs are in operation in the Changjiang River （Yangtze River） drainage area, and more are being constructed. While damming is well known to affect riverbome nutrient loads, and thus the riverine ecosystems owing to removal of carbon fixation, and removal of particles in reservoir sediments, there is limited information on the detailed early diagenesis of sediments in reservoirs including the regeneration processes of nutrients deposited in sediments and exchange flux across the sediment-water interface, which is important for mass balance of riverbome nutrients. In the present study, two large-size reservoirs, Wujiangdu Reservoir （WJDR） and Dongfeng Reservoir （DFR）, located on the main steam of the Wujiang River and with uniformity hydrography and discrepancy biogeochemical activity （e. g. primary production）, were selected for a comparative study on the detailed processes of nutrient regeneration. Water, pore water and sediment were sampled from these two reservoirs and dissolved organic carbon （DOC）, NH4^＋, NO3^-, PO4^3-, dissolved silica （DSi） in the overlying water and pore water, and total organic matter in sediments were determined. The results of correlation analysis suggested that in these two reservoirs, processes of nutrient regeneration near the sediment-water interface were significantly different. As a result of rapid decomposition of algae-derived ＂labile＂ organic matter in upper sediments, nutrient regeneration processes and upward fluxes in WJDR are dramatically stronger than those of DFR. NH4^＋ upward flux from sediment in WJDR was about 17 times higher than that in DFR. PO4^3- flux in WJDR is about 13 times above that in DFR. DOC flux in WJDR is larger that in DFR by 5 times, and DSi by 1 time.  相似文献   

益母草对钉螺化感作用的实验研究     

唐万鹏  王万贤 《湿地科学与管理》2006,2(4):47-53

本实验研究分别采用益母草的根、茎、叶的0.10、0.25、0.50、2.00、1.00g/L和水苏碱的0.20、0.40、0.60、0.80、1.00g/L5个不同浓度梯度的处理液处理钉螺,设清水和0.001g/L浓度的氯硝柳胺溶液为对照。结果表明:(1)益母草各部分的水浸液均有很好的灭螺效果,用不同浓度的处理液浸杀钉螺,在不同时间的处理下,钉螺死亡率存在差异,其钉螺死亡率是随处理浓度的增加和时间的延长呈上升趋势,0.5g/L以上的益母草根、茎、叶、化水浸液和浓度达0.60g/L以上的水苏碱处理液均可达到100%的毒杀钉螺致死效果,与通常使用浓度0.001g/L氯硝柳胺溶液的灭螺效果相当。不过益母草根、茎、叶水浸液的毒效较氯硝柳胺略慢,用0.001g/L氯硝柳胺溶液处理钉螺2～3d可达100%的死亡率,而用0.5g/L以上的益母草根、茎、叶水浸液水溶液处理需要3～5d才能达到同样的效果;灭螺效果顺序依次为:叶>茎>根。(2)钉螺趋避性研究表明,水苏碱和益母草根、茎和叶的处理液对钉螺具有明显的驱逐作用,而盐酸益母草碱几乎没有作用。(3)并对其剂量-效应与时间-效应进行了研究分析,由此获得化感作用植物益母草灭螺的化学生态学证据,为研制新的具中国特色的植物成份灭螺剂打下了基础,并为合成仿生灭螺剂以及最终构建生态工程中强化感作用植物群落灭螺提供理论依据。  相似文献