两种北极微型浮游植物的荧光差异性分析     

张芳  光应芝  张前前  何剑锋 《海洋与湖沼》2013,44(5):1189-1193

为了利用荧光技术对北极浮游植物的生理生态信息进行系统研究, 本文以实验室培养条件下的两种北冰洋微型浮游植物——柔弱伪菱形藻(Pseudo-nitzschia delicatissima)和海链藻(Thalassiosira sp.)为研究对象, 先采用流式细胞术(Flow Cytometry, FCM)和全荧光光谱法对其活体中色素的静态荧光进行测量, 后利用脉冲调制方法(Pulsed Amplitude Modulation, PAM)对其叶绿素动态荧光信息进行测定, 对两种浮游植物的三类荧光信息进行综合比较分析, 结果表明: 柔弱伪菱形藻的丰度、基础荧光(F0)、光合系统Ⅱ(PSⅡ)光化学的有效量子产量(ΦPSⅡ)及光合电子传递效率(ETR)分别为22×105cell/mL、844、0.247和42.8, 皆高于海链藻的相应值(8×105cell/mL、464、0.185及33.0); 静态荧光和基础荧光分析表明柔弱伪菱形藻细胞内叶绿素a (chl a)含量较高; 海链藻细胞内则具有较高含量的类胡萝卜素, 其对光保护机制依赖性较强。本文初步展示了三类荧光分析法在极地微型浮游植物生理生态研究方面的联合应用。  相似文献   

Introduction,preliminary handling,and diseases of grass carp in New Zealand     

D. J. Edwards  P. M. Hine 《新西兰海洋与淡水研究杂志》2013,47(3):441-454

Fry of grass carp, Ctenopharyngodon idella (Val.), were air‐freighted from Hong Kong to New Zealand in October 1971. The fish were at first kept in tanks with a recirculating water supply, where they soon showed symptoms of parasitic infestation and suffered heavy mortality.

Attempts were made to remove parasites with chemotherapeutic treatment. Tripartiella sp., Dactylogyrus ctenopharyngodonis, and Gyrodactylus clenopharyngodontis were eliminated by prolonged bathing of the infected fish with quinine. Chemical treatment failed to eliminate Ichthyophthirius mullifiliis from the body surface and the gills, but the rate of spontaneous recovery of the fish from this disease was accelerated by keeping them in clean, running water. Experiments to remove the cestode Bothriocephalus gowkongensis from the intestine of the fish with tetravalent tin compounds and to control the intermediate host (Cyclops) in the water resulted in the eradiction of the cestode.

When parasitic infestations had been brought under control the fish were transferred to outdoor ponds, where they began to feed on aquatic and terrestrial vegetation and rapidly increased in size.  相似文献   

Seston transport and deposition in Pelorus sound,south Island,New Zealand   总被引：2，自引：2，他引：0  

Lionel Carter 《新西兰海洋与淡水研究杂志》2013,47(2):263-282

Transport of seston (suspended sediment) in Pelorus Sound is controlled by tides and freshwater inflow. During high freshwater inflow, a moderately stratified estuarine circulation may be superimposed on the tidal circulation, but the latter dominates and transports seston seawards and landwards with the ebb and flood phases respectively. With extreme freshwater inflow, the estuarine circulation gains impetus and most seston is rapidly transported seaward in the low saline surface layer.

Irrespective of circulation there is a persistent trend in seston concentrations. Highest values occur at the sound's head because of the influence of nearby Pelorus and Kaituna Rivers and because of resuspension of bottom sediment by strong tidal currents. Seston concentrations wane along the sound until near the entrance, where values increase as a result of greater production of biogenic seston and because additional seston is brought in from Cook Strait with the flood tide. This trend parallels variability in the thicknesses of muddy bottom sediments. Muds are thick at the head where an extensive delta extends from the river mouths; muds gradually thin seaward and then thicken markedly in the vicinity of the sound entrance.

Seston weight and composition patterns and 3.5 kHz seismic profiles indicate Pelorus Sound acts as a double‐ended sediment trap. The upper reaches receive and retain river‐derived seston, whereas the sound entrance traps seston derived from Cook Strait. This situation appears to hold for both high and extremely high influxes of sediment.  相似文献   

Nitrogen utilisation by size‐fractionated phytoplankton assemblages associated with an upwelling event off Westland,New Zealand     

F. Hoe Chang  Warwick F. Vincent  Paul H. Woods 《新西兰海洋与淡水研究杂志》2013,47(3-4):287-301

Abstract

Uptake rates of ammonium (NH₄ ⁺ ), nitrate (NO₃ ^? ), and urea by three subgroups of phytoplankton (< 200, < 20, < 2 μm) off Westland, were measured using ¹⁵ N tracer techniques in midwinter 1988, after a recent upwelling. For all size fractions at surface irradiance (I₁₀₀), nitrogen (N) was taken up primarily as NO₃ ^?. This accounted for 67–85% of total N uptake (SρN), whereas at 40 and 7% of surface irradiance, the regenerated N (NH₄ ⁺ ) and urea) made up 31–72% of SρN. Depth profile experiments for all three size components showed that uptake of NO₃ ^? was most light‐sensitive, followed by that of NH₄ ⁺ and urea. The irradiance and nutrient availability plot indicated that light was substantially more important than the nutrient concentrations in controlling the assimilation of N by microplankton (20–200 μm). Nano‐ (2–20 μm) and picoplankton (< 2 μm) however, were not as sensitive to either light or nutrient concentrations. High winds and the resulting deep mixing, combined with offshore and alongshore advection in the midwinter, were suggested to be the major cause of the low biomass and N productivity.  相似文献   

Northern Adriatic phytoplankton response to short Po River discharge pulses during summer stratified conditions     

Damir Vilicic  Milivoj Kuzmic  Igor Tomažić  Zrinka Ljubešić  Sunčica Bosak  Robert Precali  Tamara Djakovac  Danijela Marić  Jelena Godrijan 《Marine Ecology》2013,34(4):451-466

A 275‐km‐long transversel Northern Adriatic profile from the mouth of the Po River (Italian Adriatic coast) to the Kvarner region (Croatian coastal island area) was investigated in three successive case studies in August 2008, 2009 and 2010. The short Po River pulses in August result in the surface advection of riverine water, nutrients and phytoplankton from the western to the eastern side of the Adriatic. This surface spreading exhibits inter‐annual variability depending on the riverine discharge in the preceding period. The Po River discharge pulse in August 2010 in particular resulted in an extraordinary tongue‐like advection of riverine water, nutrients, and phytoplankton towards the Eastern Adriatic coast. The phenomenon was detected using both satellite imagery and classical oceanographic measurements. In the advective water, toxic dinoflagellates were most abundant in August 2010, when the influence of the Po was greatest.  相似文献   

光谱波段宽度对森林叶片叶绿素含量反演的影响分析     

佃袁勇  方圣辉  徐永荣  张育育  刘沐阳 《测绘科学》2012,37(6):40-42

随着传感器技术的发展,高光谱数据光谱的波段宽度逐渐变窄,如何从海量的光谱数据中找到最优的光谱波段反演叶绿素含量,成为研究的难点问题。本文在测量华中农业大学狮子山6种主要树种的光谱数据和叶绿素含量的基础上,利用叶绿素指数(CI)和回归方法反演叶绿素含量,并分析了波段宽度对反演叶绿素含量结果的影响,结果发现波段宽度会影响到叶绿素反演的精度,当波段宽度为30nm时,叶绿素含量与"红边"区域(700nm-730nm)和近红外区域(770nm-800nm)叶绿素指标(CI)间的线性关系较好,决定系数可达到77.62%,均方根误差为10.6ug/cm2。  相似文献   

外源硒对罗布麻幼苗生长及光合作用的影响     

吕海祥  田长彦  王梓宇  张科 《干旱区地理》2015,38(1):83-89

硒是植物生长发育的必需元素,能促进植物生长。采用盆栽试验的方法,研究了施用不同浓度的外源硒对一年生罗布麻幼苗生长及叶绿素荧光参数的影响。研究结果表明：根施（R）和叶面喷施（L）两种施硒方式下,硒浓度与罗布麻一年生幼苗生长及叶绿素荧光参数有极显著相关性,表现为,一定浓度范围内（0～1.5 mg·kg^-1）,随着硒浓度的增加,罗布麻茎干重、叶干重、株高、茎粗、主叶对数和侧枝数均有有不同程度的上升;Fv/Fm、Fv/Fo、Ψo、φEo、PIABS、均随着施硒浓度的增加而增加;V_K、V_J、V_I均随着施硒浓度的增加而逐渐下降,S_m逐渐上升;说明施硒促进了罗布麻叶PSII的活性,提高了PSII反应中心的开放程度,增大其光能利用率。但随着供应浓度的进一步提高,各项生长及荧光指标有所降低,因此过量的硒供应对于罗布麻生长不利,而且有益与有害之间的范围很小,具体罗布麻栽培中要严格控制硒用量在1～3 mg·kg^-1范围内。搜索 #SrsZzqBAngjSTips { Z-INDEX: 999999999; POSITION: absolute; WIDTH: 56px; HEIGHT: 24px; LEFT: 1342177.27em } #SrsZzqBAngjSTips A { POSITION: relative; LINE-HEIGHT: 24px; MARGIN: -32px 0px 0px; PADDING-LEFT: 23px; WIDTH: auto; DISPLAY: block; BACKGROUND: url(http://mat1.gtimg.com/www/sogou/sogou_tips_v1.png) no-repeat 0px 0px; HEIGHT: 24px; COLOR: #000; FONT-SIZE: 12px; TEXT-DECORATION: none } #SrsZzqBAngjSTips A:hover { BACKGROUND-POSITION: 0px -34px; COLOR: #45a1ea }  相似文献   

胶州湾大沽河口潮间带沉积物中叶绿素和有机质含量的季节变化   总被引：1，自引：0，他引：1  

尹盛乐  刘晓收  袁超  谭朋  胡金  刘阳  董树刚 《海洋湖沼通报》2012,(2):97-106

为了解胶州湾大沽河口潮间带沉积物中底栖微藻和有机质含量的季节变化,于2010年4月至2011年2月,分4次(春、夏、秋、冬)测定分析了沉积物中的叶绿素含量和有机质含量,并分析了沉积物的粒度组成及现场温度。结果表明,表层(0～2cm)沉积物叶绿素a含量为0.42～6.06mg/kg,有机质含量为0.48%～3.71%;次表层(2～5cm)叶绿素a含量为0.13～3.53mg/kg,有机碳质量为0.31%～3.78%;深层(5～8cm)叶绿素a含量为0.14～1.95mg/kg,有机质含量为0.47%～3.72%。经统计检验和Pearson相关性分析得出,胶州湾大沽河口沉积物中的叶绿素a含量春冬两季最高,夏季和秋季最低;叶绿素a含量与深度和温度呈负相关;与所处潮带、有机质含量、粉砂黏土含量在本实验中均无显著的相关性;有机质含量与季节关系不大,高潮带最高,其含量与粉砂黏土含量呈正相关,与叶绿素含量、深度和温度关系不明显。  相似文献   

春末夏初巽他陆架表层水体的叶绿素粒级结构及其和营养盐的关系   总被引：1，自引：1，他引：0  

柯志新  黄良民  李刚  尹健强  谭烨辉 《海洋学报》2012,34(3):190-196

  相似文献   

The preliminary study of the high chlorophyll in the central Bohai Sea in summer   总被引：1，自引：1，他引：0  

WANG Yue  WU Dexing  LIN Xiaopei 《海洋学报(英文版)》2012,31(1):66-72

In the central Bohai Sea, both historical data and in situ observations show that the distribution of high chlorophyll concentration is consistent with warm water. The previous studies confirm that the warm water is caused by a joint effect of wave and tidal mixing. In this paper, the authors have coupled the Princeton ocean model (POM) with a biochemical model to further investigate the formation of the high chlorophyll concentration. When the wave mixing is excluded, the water temperature is lower and the chlorophyll concentration is also lower than the observation. However, if the wave mixing is included, strong wave mixing in the upper layer brings the warm water downward and nutrients upward so that both temperature and nutrients tend to be uniformly high from the surface to the bottom. This provides an environment which is favorable for the growth of the phytoplankton. Therefore the chlorophyll concentration, as a symbol of phytoplankton, shows high value in the central Bohai Sea. In conclusion, the wave mixing plays an important role in the formation of high chlorophyll concentration in the central Bohai Sea.  相似文献