Purification and characterization of lipoxygenase from Entermorpha clathrata     

YANG Wenge  XUE Changhu  XU Dalun  FU Xueyan  HE Xiong 《海洋学报(英文版)》2008,27(1):92-101

The purification and characterization of lipoxygenase （LOX, EC 1.13.11.12） from the green algae Enteromorpha clathrata were studied. Two components marked LOX - 1 and LOX - 2 were purified and their molecular masses were estimated to be 102 and 79 ku by SDS - PAGE. Both LOX - 1 and LOX - 2 were stable over the pH wide range 6.0 - 10.0 and had the optimum pH of 10.0 and 8.6, at optimum temperature of 30 and 25℃, respectively. Substrate specificities of LOX - 1 and LOX - 2 were the greatest towards linoleic acid, followed by arachidonic acid and linolenic acid. The Michaelis constant values of LOX - 1 and LOX - 2 were 0.23 and O. 20 mmol/dm^3 with the substrate of linoleic acid. The LOX activities were stimulated greatly by Ca^2＋ but inhibited by Hg2 ~ and the antioxidants such as BHA, BHT and TBHQ. The hydroperoxide products of LOX were analysed by HPLC with the substrate of methyl linoleate, and the results showed that LOX - 1 formed mainly 9-hydroperoxides while LOX - 2 formed both 9- and 13-hydroperoxides at a ratio of 24： 76.  相似文献   

PHYSIOLOGICAL INHIBITORY EFFECT OF OCS IN ARACHIDONIC ACID-RICH PARIETOCHLORIS INCISA （TREBOUXIOPHYCEAE, CHLOROPHYTA）   总被引：2，自引：0，他引：2  

刘建国  张成武ZviCohen AmosRichmond 《中国海洋湖沼学报》2002,20(3):248-255

Parietochloris incisa is an arachidonic acid-rich snow green alga. The main physiological profiles, such as ash free dry weight (AFDW), chlorophyll, carotenoid, protein and total fatty acids (TFA), in this alga exposed to old culture supernatant (OCS) at the decline phase or its crude ethyl acetate extracts (CEAE) were investigated by using tubular photobioreactors of different diameters. Results showed that both OCS and CEAE had strong inhibitory effect on the above physiological parameters. The longer the culture was exposed to OCS and the more CEAE were added into the algal culture, the more the above physiological properties were inhibited. Arachidonic acid (AA), the dominant component of fatty acids in this alga, was also seriously inhibited with respect to total TFA, AFDW of cell mass, or culture volume, due to a prebable reduction of enzymes activities catalyzing chain elongation from C18:1ω9 to AA. These results incontestably evidenced that some CEAE dissolving substances existing in OCS, like auto-inhibitors, inhibited P. incisa growth through feedback. Hence, any efficient removal of auto-inhibitors from algal culture to decrease their bioactivity could be good for maximal production of desired products like AA.  相似文献   

石灰华扇(Tufa Fan)沉积物中的微型生物多样性   总被引：3，自引：0，他引：3  

王智慧  张朝晖  李建华 《沉积学报》2008,26(4):670-675

为了探索石灰华扇沉积物中微型生物多样性特征, 作者2006年7～9月对贵州中部马脚冲瀑布石灰华扇进行了野外生态学调查,釆集样品和标本50份。经室内初步分析和鉴定, 该瀑布石灰华扇中含有4大微型生物多样性类群共25个物种,它们是蓝细菌(Cyanobacteria)1个种,原生动物(Protozoan) 16个种,绿藻(Green algae) 1个种,硅藻(Diatoms)7个种。此外,石灰华扇中还发现了苔藓植物(Bryophytes)6个种。石灰华扇沉积物的形成,是微型生物多样性-石灰华扇沉积相互作用的地质生态学结果。  相似文献   

Steroids from green alga <Emphasis Type="Italic">Chaetomorpha basiretorsa</Emphasis> Setchell     

쪷듳폀  랶쿾  쯯뷜  몫샶뻽  쪯붨릦 《中国海洋湖沼学报》2008,26(4):415-418

Six steroids have been isolated from ethanolic extract of green alga Chaetomorpha basiretorsa Setchell by a combination of repeated normal phase silica gel and Sephadex LH-20 gel column chromatography as well as recrystallization. Using spectroscopic methods including MS and NMR, their structures were determined as β-1awsaritol （1）, saringosterol （2）, 24-hydroperoxy-24-vinyl- cholesterol （3）, β-stigmasterol （4）, stigmast-4-en-3α, 6β-diol （5）, 29-hydroxystigmasta-5, 24 （28）-dien-3β-ol （6）. All these compounds were obtained from this genus for the first time and they were inactive （IC50〉10μg/ml） against KB, Bel-7402, PC-3M, Ketr 3 and MCF-7 cell lines.  相似文献   

广东常见的绿藻和褐藻     

李伟新 《广东海洋大学学报》1986,(1):70-76

广东省藻类资源丰富，常见种类有80多种。本文主要报导的常见绿藻有25种，褐藻有22种，总共47种。其中产量较大，富有经济价值的绿藻有礁膜、石莼、裂叶石莼、浒苔和长松藻等；褐藻类产量大的有马尾藻科的种类．海南岛年产马尾藻可达18—20万担(干品)，全省产量可达40多万担。它是提取褐藻胶、甘露醇、碘和马尾藻精的主要原料，也是鲍鱼养殖的良好饲料，有的绿藻椭藻也是江蓠养殖的主要敌害，因此，本文所报导的种类可供水产院校师生和水产工作者参考。  相似文献   

基于无人机与卫星图像的大型绿藻孔石莼(Ulva pertusa)遥感估算研究     

孟苗苗  郑向阳  邢前国  刘海龙 《热带海洋学报》2022,41(3):46-53

卫星影像是监测海面漂浮绿藻的重要数据源, 但是混合像元的存在使得绿藻提取存在一定的误差。想要实现近海区域底栖绿藻的精细监测, 需要解决绿藻亚像素覆盖度的问题。本文以厘米级分辨率无人机数据的绿藻提取结果为基准, 通过分析Landsat卫星影像绿藻光谱, 建立绿藻亚像素覆盖度与多种植被指数和多个特征波段反射率的反演模型。结果表明, 蓝、绿、红波段反射率与绿藻亚像素覆盖度呈现较好的线性关系, 随着绿藻亚像素覆盖度递增, 蓝、绿、红波段反射率的值均递减。将蓝、绿、红波段的三种绿藻亚像素覆盖模型进行验证, 发现绿波段反射率所建立的反演模型具有更高的准确性, 决定系数、均方根误差、平均相对误差分别为0.92%、0.07%、10.85%。本文所建立的模型可以估算大型绿藻亚像素覆盖度, 实现Landsat卫星影像对大型绿藻的精细监测。  相似文献   

富油新绿藻在不同培养期油脂的脂肪酸组成     

《广东海洋大学学报》2017,(4)

用改良SE培养基培养富油新绿藻(Neochloris oleoabundans),用缺氮改良SE培养基培养处于稳定期后期的藻细胞3 d,分别收集处于生长期、稳定期、衰亡期和缺氮胁迫状态的藻细胞,用Folch法提取藻细胞油脂,以质量法计算油脂产率,用气相色谱法测定脂肪酸组成。结果表明,富油新绿藻随着培养期延长油脂含量提高,在缺氮胁迫状态下藻细胞油脂质量分数达28.99%;随着细胞油脂含量的增加,油脂脂肪酸组成也发生变化,从生长期到氮缺乏胁迫状态油酸质量分数由24%增至44%,而亚油酸质量分数由26.84%降至19.85%,亚麻酸质量分数由12.74%降至4.75%。富油新绿藻在分批培养后期和氮缺乏而碳源充足的条件下可积累油脂,而在油脂积累阶段油脂的脂肪酸组成亦不同。  相似文献   

南极冰藻Chlorophyceae L-4抗氧化酶活性对UV-B辐射增强的响应   总被引：2，自引：0，他引：2  

缪锦来  王波  阚光锋  姜英辉  侯旭光  李光友 《海洋科学进展》2004,22(3):313-319

为了了解在UV B辐射增强的胁迫下,南极冰藻ChlorophyceaeL 4细胞内保护酶系统对活性氧自由基的清除作用,对不同辐射强度下ChlorophyceaeL 4细胞中丙二醛含量的变化、自由基的产生速率和相应的保护酶系统的变化进行了测定。结果表明,在UV B辐射增强的初期(1～3d),ChlorophyceaeL 4中丙二醛含量的迅速提高,但随后很快降低,在低强度UV B(35μW·cm-2)辐照下保护酶活性能够恢复到基本水平。在UV B辐射增强的胁迫下,ChlorophyceaeL 4中的超氧化物歧化酶、过氧化物酶和过氧化氢酶的活性明显比对照组的高,而且在高强度(70μW·cm-2)的辐照下,酶活性更高。ChlorophyceaeL 4中的抗坏血酸过氧化物酶的活性在UV B辐射增强的胁迫下略有升高或变化不大。本研究结果表明,保护酶系统在南极冰藻适应南极强辐射环境中起着非常重要的作用。  相似文献   

Sequences and Phylogeny Analysis of rbcL Gene in Marine Chlorophyta     

SHEN Songdong  LI Yanyan  WU Xunjian  DING Lanping 《青岛海洋大学学报(英文版)》2010,(2):145-150

  相似文献   

西沙群岛原绿藻的超微构造研究     

孙爱淑  曾呈奎 《海洋与湖沼》1998,29(4):347-352

于1981年3月在西沙群岛永兴岛采集原绿藻标本。用光镜和电镜对所采原绿藻进行研究。结果表明,原绿藻为简单的原核、单细胞藻类,在同一生境的相同宿主体表有4种不同的细胞构造类型：第1种类型,中央大“液泡”,类囊体单条、平行分散于周围区的细胞质中;第2种类型,中央是大“液泡”,周围区内类囊体多,并形成垛叠;第3种类型,许多小泡充满细胞的大部分,类囊体穿插于小泡之间;第4种类型,有中央大“液泡”,还有许多小泡分散在细胞周围区。上述分类以类囊体和“液泡”的结构和分布为主要分类依据,反映出不同结构的进化意义。表明中国西沙群岛有丰富的原绿藻资源。  相似文献