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1.
CHAN Zhuhua CHEN Xinglin HOU Yanping GAO Boliang ZHAO Chungui YANG Suping ZENG Runying 《海洋学报(英文版)》2018,(2)
Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase. 相似文献
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垂直探测台风边界层特征对于认清台风结构具有重要意义。基于1319号台风“天兔”途经的三个边界层风廓线雷达站的观测资料,结合探空数据,本文分析了“天兔”的边界层径向、切向风特征,结果表明:1)最大切向风速出现在眼区附近,在“天兔”稳定维持为强台风级别期间,最大切向风速基本稳定在1800 m高度左右,随着登陆后强度的迅速减弱,最大切向风速减弱、最大切向风速垂直范围缩小;2)最大风速所在高度和台风入流层顶高基本相近,大于依据理查森数或位温梯度所判断出的边界层高度,而基于信噪比(SNR)或其梯度所判断的混合层高度时常偏低;3)“天兔”登陆前边界层高度可以达到2100 m以上,在台风级别及以上时,各站观测到的边界层高度变化不大,基本在1200~1600 m之间,登陆后随着台风强度的减弱,边界层高度迅速减小。 相似文献
4.
During the pre-summer rainy season, heavy rainfall occurs frequently in South China. Based on polarimetric radar observations, the microphysical characteristics and processes of convective features associated with extreme rainfall rates(ERCFs) are examined. In the regions with high ERCF occurrence frequency, sub-regional differences are found in the lightning flash rate(LFR) distributions. In the region with higher LFRs, the ERCFs have larger volumes of high reflectivity factor above the freezin... 相似文献
5.
Valuable dropsonde data were obtained from multiple field campaigns targeting tropical cyclones, namely Higos,Nangka, Saudel, and Atsani, over the western North Pacific by the Hong Kong Observatory and Taiwan Central Weather Bureau in 2020. The conditional nonlinear optimal perturbation(CNOP) method has been utilized in real-time to identify the sensitive regions for targeting observations adhering to the procedure of real-time field campaigns for the first time. The observing system experiments... 相似文献
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以从海南三亚近海海水中采集的样品为研究对象,利用平板筛选法筛到一株产酯酶的中度嗜盐菌菌株LYG1-1。通过形态学、生理生化及16S rRNA序列分析,发现该菌株为革兰氏阴性菌,其最适生长温度为30℃,最适生长pH为7.5,最适NaCl浓度为10%。16S rRNA基因序列分析显示,菌株LYG1-1与Halomonas salina亲缘性最近,16S rRNA基因序列相似性为99.72%。在含10%NaCl的0.05mol·L-1Tris-HCl(pH 8.0)缓冲液中,45℃条件下作用,酶活性最高,40℃处理24h仍保持83%的活性。金属离子浓度为5mmol·L-1时,Ca2+、Mg2+对菌株LYG1-1酶活具有明显的激活作用,Hg2+表现出强烈的抑制作用。 相似文献
7.
Temporal and spatial distribution characteristics of sea surface wind in Guangdong''s coastal areas were analyzed with data from four offshore observational stations between 2012 and 2015. The results are shown as follows: (1) The probability distribution of wind speed was basically consistent with Gaussian distribution characteristics; winds of Beaufort force 6 or higher were observed mainly in far offshore stations from October to March. (2) The probability distribution of wind direction was represented well by Weibull distribution. The deviation of wind direction of far station was relatively small for it was mainly controlled by monsoon over the South China Sea, while the near offshore station had a relatively large diurnal variation because of the influence of local synoptic systems such as sea-land breeze. (3) There were significant seasonal differences in wind speed and direction observed by different offshore observational stations. In strong wind seasons, the deviation of wind direction was relatively small while the deviation of wind speed was relatively large, and vice versa. In contrast with Class I station, the other three stations exhibited approximately normal distribution of wind direction and wind speed deviations. (4) Wind direction diurnal variation was moderate in windy periods, while it was obvious in relatively lower speed conditions. The deviation of wind speed in windy periods was generally greater because it was influenced by mesoscale weather systems for 10-20 h, and the influence was complicated, resulting in greater local differences in wind speed. 相似文献
8.
中国东南大陆晚侏罗世地层普遍缺失,仅零星见于个别地区,香港新界东北的荔枝庄组即为其一。荔枝庄组出露于香港世界地质公园沉积岩园区的荔枝庄地区,自下往上由火山岩—沉积岩—火山岩组合而成,沉积岩中发育大型包卷层理和滑塌构造等典型沉积构造,是香港地区最具代表性的晚侏罗世火山—沉积岩系。通过实测地层剖面研究,确定其成岩过程大体上可划分为早期普林尼式火山爆发、中期破火山口湖相沉积和晚期普林尼式火山爆发三个阶段,以湖相沉积作用为主、火山喷发作用为辅;受晚期火山岩浆活动的影响,沉积岩层普遍发生硅化或炭化。荔枝庄组独特的岩石组合与形成的古地理环境,为探讨中国东南大陆中生代火山活动—沉积作用方式与成岩过程,提供了难得的研究实例。 相似文献
9.
Mesoscale Vortex Generation and Merging Process: A Case Study Associated with a Post-Landfall Tropical Depression 总被引:5,自引:0,他引:5
An observational analysis of satellite blackbody temperature (TBB) data and radar images suggests that the mesoscale vortex generation and merging process appeared to be essential for a tropical-depression-related heavy rain event in Shanghai, China. A numerical simulation reproduced the observed mesoscale vortex generation and merging process and the corresponding rain pattern, and then the model outputs were used to study the related dynamics through diagnosing the potential vorticity (PV) equation. The t... 相似文献
10.
Reconstructed light extinction coefficients using chemical compositions of PM<Subscript>2.5</Subscript> in winter in Urban Guangzhou,China 总被引:8,自引:0,他引:8
The objective of this study was to reconstruct light extinction coefficients (b ext ) according to chemical composition components of particulate matter up to 2.5 μm in size (PM 2.5 ). PM 2.5 samples were collected at the monitoring station of the South China of Institute of Environmental Science (SCIES, Guangzhou, China) during January 2010, and the online absorbing and scattering coefficients were obtained using an aethalometer and a nephelometer. The measured values of light absorption coefficient by particle (b ap ) and light scattering coefficient by particle (b sp ) significantly correlated (R 2 > 0.95) with values of b ap and b sp that were reconstructed using the Interagency Monitoring of Protected Visual Environments (IMPROVE) formula when RH was <70%. The measured b ext had a good correlation (R 2 > 0.83) with the calculated b ext under ambient RH conditions. The result of source apportionment of b ext showed that ammonium sulfate [(NH 4 ) 2 SO 4 ] was the largest contributor (35.0%) to b ext , followed by ammonium nitrate (NH 4 NO 3 , 22.9%), organic matter (16.1%), elemental carbon (11.8%), sea salt (4.7%), and nitrogen dioxide (NO 2 , 9.6%). To improve visibility in Guangzhou, the effective control of secondary particles like sulfates, nitrates, and ammonia should be given more attention in urban environmental management. 相似文献