The extracellular polysaccharide hydrolase-producing strain EP-1 was isolated from seawater and identified as Paenibacillus pabuli. Furthermore, a homogeneous extracellular polysaccharide hydrolase from Paenibacillus pabuli EP-1 was purified by combining ion-exchange chromatography and size exclusion chromatography with a purification fold of 90.69 and recovery of 16.23%. Characterization of the purified polysaccharide hydrolase revealed a molecular mass of 38 k Da and optimum activity at 45℃ and pH 6.0. The polysaccharide hydrolase maintained its stability within a wide range of pH(3.0–12.0) and thermal stability when the temperature was below 50℃. The presence of Hg~(2+), Fe~(2+), Mn~(2+), Co~(2+) and SDS notably decreased hydrolase activity, and organic solvents such as formaldehyde, acetone, DMF and acetonitrile completely inhibited hydrolase activity. The purified hydrolase had no activity on agar, carrageenan, gellan gum, sodium alginate, or starch, but effectively hydrolyzed the polysaccharide from Ulva prolifera. The Km and Vmax values of this hydrolase were 43.84 mg m L~(-1) and 4.33 mg m L~(-1) min~(-1), respectively. The sequence analysis with quantitative time-of-flight mass spectrometry indicated that the hydrolase was an endoglucanase. 相似文献
碳酸盐岩风化吸收的大气CO2主要以HCO3-形式连续地经由河流从大陆输送到海洋,成为陆地生态系统的重要碳汇。目前主要河流流域的碳酸盐岩风化碳汇估算存在不确定性,分布格局尚不清晰。基于GEMS-GLORI全球河流数据库提供的全球10万km 2以上主要河流流域多年平均监测数据,利用水化学径流法估算出全球主要河流流域碳酸盐岩对CO2的吸收速率为0.43±0.15 Pg CO2 yr -1,平均CO2吸收通量为7.93±2.8 t km -2 yr -1。CO2吸收通量在不同气候带下差异显著,热带和暖温带CO2年吸收速率占全球主要河流流域年吸收速率的62.95%。冷温带CO2年吸收速率占全球主要河流流域的33.05%,仅次于热带地区。本文划分出全球CO2吸收通量的9个关键带,关键带的交汇处CO2吸收通量较高。喀斯特出露流域碳酸盐岩对CO2吸收通量的均值为8.50 t km -2 yr -1,约为非喀斯特流域的3倍。全球喀斯特出露流域碳酸盐岩风化碳汇在全球碳循环、水循环及碳收支平衡估算研究方面占据重要地位。 相似文献
In many arid ecosystems, vegetation frequently occurs in high-cover patches interspersed in a matrix of low plant cover. However, theoretical explanations for shrub patch pattern dynamics along climate gradients remain unclear on a large scale. This context aimed to assess the variance of the Reaumuria soongorica patch structure along the precipitation gradient and the factors that affect patch structure formation in the middle and lower Heihe River Basin (HRB). Field investigations on vegetation patterns and heterogeneity in soil properties were conducted during 2014 and 2015. The results showed that patch height, size and plant-to-patch distance were smaller in high precipitation habitats than in low precipitation sites. Climate, soil and vegetation explained 82.5% of the variance in patch structure. Spatially, R. soongorica shifted from a clumped to a random pattern on the landscape towards the MAP gradient, and heterogeneity in the surface soil properties (the ratio of biological soil crust (BSC) to bare gravels (BG)) determined the R. soongorica population distribution pattern in the middle and lower HRB. A conceptual model, which integrated water availability and plant facilitation and competition effects, was revealed that R. soongorica changed from a flexible water use strategy in high precipitation regions to a consistent water use strategy in low precipitation areas. Our study provides a comprehensive quantification of the variance in shrub patch structure along a precipitation gradient and may improve our understanding of vegetation pattern dynamics in the Gobi Desert under future climate change.
This paper focuses on the effects of two types of El Niño events on tropical cyclone activity. We classified El Niño events from 1961 to 2015 according to their sea surface temperature (SST) anomalies into an eastern type and a central type. Then we selected strong tropical cyclones to statistically analyze the tropical cyclone characteristics during different events and their effects, as well as to study the possible mechanisms related to thermodynamic and dynamic factors. The tropical cyclone generation areas were found to be very similar during the two kinds of events. The average number of tropical cyclone in the eastern event is more than that in central event, and the hurricane in northeastern Pacific (HNP) has more energy than the typhoon in northwestern Pacific (TNP) in all cases. The seasonal distribution of the TNP high-incidence centers during central El Niño events is opposite to that of the HNP. The TNP accumulated cyclone energy (ACE) intensity is similar in the fall and summer, and the HNP ACE intensity in the summer is greater than that in the fall. The SSTs are consistent with the TNP and HNP movement trends. The Walker circulation intensity was strongly affected by the eastern events, but it quickly returned to its normal state, while the intensity was slightly reduced in the central events, and it slowly returned to its normal state. The vertical velocity distributions in the Pacific are different at different stages of both events, and the distributions of vertical velocity anomalies for typhoons and hurricanes are consistent. 相似文献
