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Carole Gaüzère Marina Moletta‐Denat Faisl Bousta Stéphane Moularat Geneviève Orial Sébastien Ritoux Jean‐Jacques Godon Enric Robine 《洁净——土壤、空气、水》2013,41(3):226-234
Biological aerosols from air constitute a significant source of exposure to microorganisms in public places. Airborne microorganisms are involved in the development of certain respiratory symptoms, allergies, or infections among users and occupants. Various sampling instruments have commonly been used in aerobiology to collect bacteria and fungi suspended in the air. The objective of this study was to develop a reliable procedure for sampling in indoor public environments presenting different levels of occupancy, airborne bacteria and fungi to be subjected to molecular analysis (bacteria and fungi quantitative PCR, capillary electrophoresis single strand conformation polymorphism fingerprinting). Four different sampling devices were tested in situ in an office building (open‐plan type) and the sampling strategy chosen was tested in two museum contexts. In accordance with the drawbacks involved to our study (quantitative and qualitative aspects, cost, and overcrowding), cyclone device appeared to be most suitable. The results underline the effectiveness of this high‐volume aerosol sampling device for both qualitative and quantitative molecular analysis. Four in situ sampling collections were carried out in 1 day in the Louvre Museum to study quantitative and qualitative variations of airborne bacterial and fungal diversity. The quantitative results revealed a similar order of magnitude for the numbers of both bacteria and fungi. In the Louvre Museum, the samples yielded between 3.7 × 104 and 4.1 × 104 genome equivalent (GE) bacteria/m3 air and between 5.0 × 104 and 5.9 × 104 GE fungi/m3 air and in the Decorative Arts Museum between, 2.1 × 104 and 2.5 × 104 GE bacteria/m3 air and between 1.4 × 104 and 1.7 × 104 GE fungi/m3 air. The results also indicate that the dominant bacterial community displayed a stable structure over a short period of time whereas dominant eukaryotic airborne community appeared more variable. 相似文献
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Anna Gotkowska‐Płachta Zofia Filipkowska Ewa Korzeniewska Wojciech Janczukowicz Beverly Dixon Iwona Gołaś Damian Szwalgin 《洁净——土壤、空气、水》2013,41(5):429-436
Experiments were conducted to study the airborne microbial contamination generated by a wastewater treatment plant (WWTP). Aerosol samples were collected simultaneously, by sedimentation and impact methods, from the area and the surroundings of the WWTP. Total colony forming units (CFUs) of heterotrophic bacteria (HPC), as well as members of the Enterobacteriaceae, staphylococci, enterococci, actinomycetes, and microscopic fungi were determined. Bacterial (HPC) concentrations ranged between 101 and 104 CFU/m3, fungi 0 and 104 CFU/m3. Higher numbers of HPC bacteria in air samples were observed in summer, fungi in autumn. The main emission of microorganisms to atmospheric air was from the mechanical sewage treatment devices of the WWTP. The facilities of the biological sewage treatment of the plant did not generate large amounts of bioaerosols. In the air obtained from the premises of the WWTP, 25 species of the Enterobacteriaceae were isolated (Salmonella spp., Klebsiella pneumoniae, Escherichia coli). At the fence and in the surroundings only Pantoea spp. were identified. This suggests that the sewage bacteria were mainly discharged in the area of the WWTP. The presence of enteric bacteria, especially Enterobacteriaceae reflects the level of air pollution with bioaerosols from sewage and is an important factor during monitoring the quality of the air around WWTPs. 相似文献
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Airborne Fungal Spores of Subalpine Zone of the Karkonosze and Izerskie Mountains （Poland） 总被引：4，自引：1，他引：3
The incidence of the airborne fungal spores was determined in the air of subalpine zone of the Karkonosze Mountains and of the Izerskie Mountains in the borderland between Poland and the Czech Republic. The experiment was conducted in 2011 and 2012 at three to four week intervals from May to October. Air samples were taken from three locations in the Karkonosze Mts. and one from the Izerskie Mts. To examine the air, the Air Ideal 3P sampler and acidified PDA medium were used. The results show that Cladosporium cladosporioides was the most abundant spore type in all the sampling locations （up to 30%）, followed by Alternaria alternata （16%-20%）, Fusarium （up to 10%） and Sclerotinia sclerotiorium （up to 6%）. The lower spore counts were recorded in May samples, compared to the other months. In this case the snow cover, that was still present in the area at the beginning of May, may be the reason for the lower, compared to June, July and August samplings, CFU （Colony Forming Unit） counts in that month.. The influx of air masses from SE, S and SW sectors in the area under study may affect dissemination of the plant-pathogenic fungi from the Czech Republic and from the South of Europe in general. 相似文献
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