High-throughput sequencing of 16S r RNA amplicons characterizes gut microbiota shift of juvenile sea cucumber Apostichopus japonicus feeding with three antibiotics     

ZHAO Ye  WANG Qing  LIU Hui  LI Bingjun  ZHANG Hongxia 《中国海洋湖沼学报》2019,(5)

Sea cucumber Apostichopus.japonicus is an important marine economic species in Asian countries due to its profound nutritional and medicinal value.So far,with the rapid development of intensified artificial aquaculture of sea cucumbers,the use of antibiotics is still an inexpensive and dispensable way to treat pathogenic infections,especially during the nursery phase.However,there is little information on the effects of antibiotics on the intestinal microbiota of sea cucumber.Therefore an Illumina based sequencing method was used to examine the intestinal bacterial composition of juvenile A.japonicas following diets with three typical antibiotics(tetracycline,erythromycin.,and norfloxacin)under 15,30,and 45 d.The findings reveal that different antibiotics have distinct effects on the growth performance of juvenile sea cucumbers.However,the richness and diversity of microbiota were barely affected by antibiotics but the community composition alterations indicated that the three antibiotics exhibited their respective patterns of reshaping the intestinal bacteria of juvenile sea cucumbers.In common,the abundance of some sensitive genera with helpful functions,such as Thalassotalea,Shewanella,Sulfitobacter,and Halomonas decreased significantly with exposure to antibiotics and the abundance of multiple potential pathogenic-and suspected antibiotic-resistant microorganisms like Arcobacter,Leucothrix,and Clostridium_sensu_stricto_1 was found increased significantly in the antibiotic groups.These results suggest that low doses of antibiotics could affect the composition of the intestinal microbiota of sea cucumbers and might increase the risk of infection of the hosts.This study could help us to explore how antibacterial compounds modify the gut microbiota of sea cucumbers and provide theoretical guidance in hatchery management by scientific antibiotic use in sea cucumber mariculture.  相似文献   

多源空间数据融合的城市精细化管理     

左海龙  罗红霞 《测绘通报》2019,(12):108-111,151

城市管理体量不断增大且管理对象关联繁多，已经成为影响城市可持续发展的重要问题。在全国智慧城市建设的大背景下，引用新技术提升城市精细化管理水平可以使城市的各管理单元协同、精确、及时地交换信息，形成智慧城市管理的配套方案，让有限的管理资源发挥出最大的使用效能，实现城市管理的最优化目标，促进经济的可持续发展。本文提出了地理信息系统、GPS、遥感、RFID等空间数据融合的技术方案，强调在空间信息和移动信息管理的基础上通过工作流和过程化管理，提高城市精细化管理的综合水平，为实现民众参与监督城市管理和智慧城市建设提供参考。  相似文献   

华东地区月平均气温统计降尺度方法比较   总被引：1，自引：0，他引：1  

高红霞  汤剑平 《气象科学》2015,35(6):760-768

用中国地面气象观测站的逐日气温观测资料和NCEP/NCAR再分析资料,分别使用基于多元线性回归(MLR)和3种主成分分析(PCA)的统计降尺度方法,对1959-2008年的华东地区的月平均气温分两个时段进行统计降尺度分析并加以检验,比较了不同降尺度方法的结果。结果表明:对于华东地区气温的统计降尺度预报,基于MLR的统计降尺度方法相对于3种PCA方法而言,对单站年际变化模拟方面有一定优势。PCA方法应用于统计降尺度时,预报因子的区域选择是影响统计降尺度结果的重要因素之一。对于温度进行统计降尺度分析时,预报因子中包含温度因子是非常必要的;所试验的4种降尺度方法,对各站点多年平均情况的模拟要好于对区域平均的年际变化的模拟。  相似文献   

Morphological and genetic comparison of two strains of a Prorocentrum species isolated from Zhejiang coastal water of China and Masan Bay of Korea     

½����  ����ϼ  �ƺ���  ��ƽ  ���η� 《中国海洋湖沼学报》2011,29(4):832-839

In this paper, we examined the detailed morphology of two strains of Prorocentrum isolated from the coastal waters of Zhejiang (Wenling area), China, and Masan Bay of Korea. A taxonomic comparison was made among strains on the basis of morphological and molecular data. The cellular dimensions of the Chinese Wenling strain (LAMB090508) and Korean strain (PDKS0206) were similar and the cells of both strains were of asymmetric and elongated shape. The posterior end of most cells was rounded. Megacytic zones of aged cells were broader with dense tiny knobs. The roundish nucleus was located in the posterior part of the cell. A few irregular shaped chloroplasts were distributed within the cell. The nucleotide similarity of the two strains, determined from the 5.8S rDNA-ITS sequences, was 99.83%. The comparative results of morphology and molecular analysis suggest that both strains isolated from China and Korea were the identical species, Prorocentrum donghaiense Lu.  相似文献   

Effects of heavy metal ions (Cu2+, Pb2+ and Cd2+) on DNA damage of the gills, hemocytes and hepatopancreas of marine crab, Charybdis japonica     

Luqing Pan  Na Liu  Hongxia Zhang  Jing Wang  Jingjing Miao 《中国海洋大学学报(英文版)》2011,10(2):177-184

There are rising concerns about the hazardous effects of heavy metals on the environment. In this study, comet assay and DNA alkaline unwinding assay were conducted on the tissues (gills, hepatopancreas, and hemocytes) of Charybdis japonica in order to illustrate genotoxicity of three heavy metal ions (Cu2+, Pb2+, and Cd2+) on the marine crabs C. japonica. The crabs were exposed to Cu2+ (10, 50, and 100 ?g.L?1), Pb2+ (50, 250, and 500 ?g L?1) and Cd2+ (5, 25, and 50 ?g L?1), and the tissues were sampled at days 0.5, 1, 3, 6, 9, and 15. DNA alkaline unwinding assay was used for testing the DNA single strand break in gills and hepatopancreas and comet assay was employed for testing the DNA damage in hemocytes. The results showed that the DNA damage (F-value) of gills in the crabs exposed to the three heavy metals was decreased gradually during the exposure periods and there was a dose-time response relationship in certain time, suggesting that the levels of DNA single strand break in all the experimental groups increased significantly compared to the controls. Changes of F-value in hepatopancreas of the crabs exposed to the three heavy metals were similar to those in gills except that the peak values were found in the 500 ?g L?1 Pb2+ treatment group at day 3 and the 50 ?g L?1 Cd2+ treatment group at day 9. The ranks of DNA damage in gills and hepatopancreas induced by the three heavy metal ions (50 ?g L?1, day 15) were Cd2+ >Pb2+ >Cu2+ and Pb2+ >Cu2+ >Cd2+. The levels of DNA damage in gills were higher than those in hepatopancreas in the same experimental group. It can be concluded that indices of DNA damage can be used as the potential biomarkers of heavy metal pollution in marine environment.  相似文献   

Influence of flow velocity and spatial heterogeneity on DNAPL migration in porous media: insights from laboratory experiments and numerical modelling   总被引：1，自引：0，他引：1  

Fei Zheng  Yanwei Gao  Yuanyuan Sun  Xiaoqing Shi  Hongxia Xu  Jichun Wu 《Hydrogeology Journal》2015,23(8):1703-1718

  相似文献   

安徽宣城红土微生物GDGTs分布特征及其古环境意义   总被引：1，自引：0，他引：1       下载免费PDF全文

马瑞元  彭红霞  张林  顾延生  胡圣虹 《地球科学》2015,40(5):863-869

中国南方更新世红土是古气候环境变化研究非常重要的载体之一,红土微生物类脂分子是其古气候环境研究的有效指标,但其在红土中的古气候意义需要更进一步的挖掘和明确.选取处于气候变化敏感地带并且已有很好年代学基础的安徽宣城红土剖面进行详细的野外调查和系统的样品采集,利用改善后的碱式水解法提取红土微生物类脂分子,通过对比分析、比较印证等方法对其中的甘油二烷基链甘油四醚(glycerol dialkyl glycerol tetraethers,简称GDGTs)进行系统研究.宣城剖面GDGTs分布特征显示:剖面下部各指标呈旋回性变化、而上部相对稳定,表明形成初期环境比较动荡,而后期成土环境相对稳定;土壤pH为7.0~8.0,推测其物源主要来源于北方干旱区或长江中下游干涸河滩沉积;BIT指标反映宣城地区在130 ka BP左右气候极为干旱.结果表明,红土中微生物类脂物GDGTs能够定量、高分辨率地重建安徽宣城的古气候环境,具有重要的研究意义.   相似文献   

孟加拉盆地东部褶皱带差异构造变形及成因     

唐鹏程  丁梁波  马宏霞  王雪峰  邵大力 《海相油气地质》2017,22(2)

基于野外地质调查和地震资料分析,证实了孟加拉盆地东部褶皱带发育底部和中部两套滑脱层。底部滑脱层在整个褶皱带均发育,而中部滑脱层仅发育于北段—中段的陆上和陆架区,在中段的陆坡-洋盆区及南段则不发育。中部滑脱层的发育受古陆坡富泥沉积控制。北段—中段主要发育滑脱褶皱及被断层突破的滑脱褶皱,而南段主要发育泥底辟和泥火山构造。孟加拉盆地东部褶皱带滑脱层系及构造样式的差异变形特征主要受区域挤压作用、沉积作用以及地层流体超压等方面存在着差异的共同影响。  相似文献   

2008年夏季楚科奇陆架海域近底层水温大幅快速变化事件研究   总被引：1，自引：1，他引：0  

舒启  乔方利  陈红霞 《海洋学报》2012,34(1):57-63

2008年夏季中国第3次北极科学考察期间,利用锚碇潜标对北冰洋楚科奇陆架海域进行了为期33 d的海流剖面、近底层温度与盐度连续观测。观测数据显示楚科奇陆架海域近底层海水温度出现了两次较大幅度的快速升降现象。结合此次科学考察R断面温盐深仪(CTD)观测资料、以及卫星遥感海表温度(SST)和海表风场等资料,综合分析表明:观测到的这种快速升、降温现象不仅发生在近底层;这种快速升、降温现象应该是由海水温度锋面在夏季整体缓慢北移的同时存在短暂南北摆动所导致;温度锋面的季节性北移属于北极气候特征,而温度锋面短暂的南北摆动则与短期天气过程有关。  相似文献   

轻非水相液体污染源区结构的影响因素数值分析          下载免费PDF全文

陶佳辉  施小清  康学远  徐红霞  吴吉春 《水文地质工程地质》2018,(6):132-132

轻非水相液体（light non-aqueous phase liquid,LNPAL）在地下介质中的运移分布与残余捕获受多种因素影响和控制。LNAPL污染场地概念模型中一般视LNAPL从地表泄漏后穿过包气带至潜水面。然而地下介质的非均质性与包气带含水量的空间变异分布可形成复杂的LNAPL污染源区结构,LNAPL可能无法到达潜水面,而在毛细水带蓄积。文章基于数值模型综合分析了LNAPL泄漏量、介质非均质性与含水量空间变异分布、潜水面周期性变化等多种因素对LNAPL污染源区结构的影响。研究表明:（1）当泄漏量较大时,LNAPL可运移至潜水面;（2）当泄漏量较小时,对于上粗下细的层状非均质条件,LNAPL可能在毛细水带边缘发生蓄积,无法到达潜水面;（3）包气带中黏土透镜体并非都是LNAPL运移的阻碍,LNAPL可以穿透低含水量的黏土透镜体,只有高含水量的黏土透镜体才对LNAPL的入渗有阻碍作用;（4）潜水面周期性变化将导致污染范围扩大。  相似文献