水稻(Oryza sativa L.)秸秆浸提液对铜绿微囊藻(Microcysti aeruginosa)的抑制作用          下载免费PDF全文

张余霞  张玲  高兴  王立新  陆长梅  吴国荣 《湖泊科学》2007,19(4):479-484

用刚收割的水稻(Oryza sativa L.)秸秆浸提液代替水配制培养基培养铜绿微囊藻(Microcystic aeruginosa),追踪测定微囊藻的生物量、叶绿素a含量、光合速率、呼吸速率、膜透性、超氧物歧化酶(SOD)活性、光合膜自发荧光强度等相关生理生化指标的变化,研究水稻秸秆浸液对铜绿微囊藻的抑制作用.结果表明,质量比≥1/100(水稻秸秆/水)的水稻秸秆浸提液对微囊藻的生长有明显的抑制作用,表现如下:藻细胞生物量在实验过程中逐日降低,藻细胞叶绿素a含量和光合速率急剧下降,呼吸速率和超氧物歧化酶(SOD)活性呈先升高后下降的趋势,膜透性迅速升高,细胞光合膜自发荧光强度显著衰减.用不同的有机溶剂对该浸提液进行萃取,浓缩后用滤纸片法在固体培养基上做抑藻实验,乙醚和乙酸乙酯萃取液能明显看到抑藻圈,证实其中含有抑制物质.  相似文献   

瘤背石磺室内温箱养殖试验   总被引：6，自引：2，他引：6  

黄金田  张余霞 《海洋科学》2004,28(10):14-16

为了探索人工养殖的方法，在实验室温箱条件下对瘤背石磺(Onchidiwns stnuma)的摄食条件及适口饵料进行了研究。试验结果表明，瘤背石磺最适摄食条件及饵料为：控制温箱温度22～26℃；控制湿度接近100％；采用人工饵料平台，即海边泥土涂上螺旋藻。  相似文献   

Growth and antioxidant status of oriental river prawn <Emphasis Type="Italic">Macrobrachium nipponense</Emphasis> fed with diets containing vitamin E     

赵卫红  王资生  於叶兵  齐志涛  吕林兰  张余霞  吕富 《中国海洋湖沼学报》2016,34(3):477-483

A feeding trial was carried out to investigate the dietary vitamin E requirement of the oriental river prawn Macrobrachium nipponense (weight of 0.3–0.4 g) and its effect role on antioxidant activity. Prawns were fed with seven levels of vitamin E (0, 25, 50, 75, 100, 200, and 400 mg/kg diet) for 60 days. The results show that dietary vitamin E supplementation could significantly increased the prawn weight (P < 0.05). The activity of superoxide dismutase (SOD) in the hepatopancreas was significantly higher in prawns fed with diets supplemented with ≤75 mg/kg vitamin E than in those fed with diets supplemented with 100–400 mg/kg vitamin E (P < 0.05). The activity of catalase (CAT) in the hepatopancreas decreased significantly as dietary vitamin E supplementation increased (P < 0.05), and no significant difference was detected in glutathione peroxidase (GSH-Px) activity between different dietary groups (P >0.05). The contents of vitamin E in the hepatopancreas and in the muscle increased with increasing dietary vitamin E. There was a linear correlation between the vitamin E level in diet and that in muscle, and between the vitamin E level in diet and that in the hepatopancreas. All the above results indicated that dietary vitamin E can be stored in the hepatopancreas and muscle and lower both the activities of SOD and CAT in the hepatopancreas, suggesting that it is a potential antioxidant in M. nipponense. Broken line analysis conducted on the weight gains of prawns in each diet group showed that the dietary vitamin E requirement for maximum growth is 94.10 mg/kg.  相似文献