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为研究不同盐度对文蛤呼吸代谢的影响,本实验设置5个盐度(‰)梯度(11、18、25、32、39),检测不同盐度对文蛤(Meretrixmeretrix)耗氧和排氨的影响,以及文蛤的外套膜、鳃、肝胰腺三种组织中乳酸脱氢酶和Na+/K+-ATP酶活性的变化。结果表明:随着盐度的不断升高,文蛤耗氧率先升后降再升,在盐度18时达到最大值;排氨率先升后降,在盐度32时达到最大值。随着盐度不断升高和胁迫时间延长,文蛤的肝胰腺中乳酸脱氢酶活力总体呈先升高后下降再升高的趋势(P0.05),酶活力在盐度39时为最高;随着盐度不断升高和胁迫时间延长,文蛤的外套膜中Na+/K+-ATP酶活力总体呈先下降再升高后下降的趋势(P0.05),在盐度32时为最高;文蛤的外套膜和鳃中乳酸脱氢酶活力以及鳃和肝胰腺中Na+/K+-ATP酶活力受盐度影响不显著(P0.05),酶活力变化也多呈现"W"形的变化趋势。研究结果为文蛤的人工养殖提供参考。 相似文献
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厌氧折流板反应器中酸化菌活性研究 总被引：5，自引：0，他引：5
本实验将厌氧折流板反应器控制在水解酸化阶段,以比INT脱氢酶活性来表征反应器中各室内的酸化菌活性。探讨了各格室酸化菌活性的变化规律,并对影响各室酸化菌活性的影响因素进行了分析。结果表明,在有机负荷2.4 kgCOD/(m3.d)条件下,反应器中各室酸化率与酸化菌比INT脱氢酶活性成相关性,一室、二室的酸化率占总酸化率的73%;同一格室内比INT脱氢酶活性中部高于底部。 相似文献
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中华哲水蚤不同地理种群苹果酸脱氢酶(MDH)的比较 总被引：7，自引：0，他引：7
中华哲水蚤 (ＣａｌａｎｕｓｓｉｎｉｃｕｓＢｒｏｄｓｋｙ)是属于甲壳纲 (Ｃｒｕｓｔａｃｅａ)桡足亚纲 (Ｃｏｐｏｐｅｄａ)哲水蚤目（Ｃａｌａｎｏｉｄａ）哲水蚤属（ＣａｌａｎｕｓＬｅａｃｈ）的一类浮游动物 ,为暖温带种 ,广泛分布于我国渤、黄海和东海沿岸区 ,为这些水域的优势种［１］。有关中华哲水蚤的研究国内有过许多报道 :李少菁［２］、陈清潮［３］、林元烧等［４］曾分别对该种类生活习性、摄食、生殖及生活史进行过研究 ,林元烧、曹文清等［５］也曾对厦门港不同月份采集的中华哲水蚤染色体组型进行了分析。而不同地理种群… 相似文献
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日本沼虾黑鳃病几种同工酶的变化与病理分析 总被引：10，自引：0，他引：10
酯酶（EST），过氧化物酶（POD），乳酸脱氢酶（LDH）和苹果酸脱氢酶（MDH）同工酶在日本沼虾中存在着组织和器官的特异性，患黑鳃病后酶带发生显著变化。在肌肉、肝脏、心脏和鳃中病虾较健康虾的LDH酶谱分别缺失了2、6、2和1条酶带，同时肝脏中新增了1条酶带；POD酶谱分别缺失了0、7、4和2酶带，肌肉中新增了2条酶带；EST酶谱分别缺失了0、2、3和1条酶带，肌肉、心脏和鳃中分别新增了1、2和2条酶带；MDH酶谱分别缺失了0、2、1和1条酶带，肌肉和脏脏中各新增了1条酶带。 相似文献
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Nannochloropsis oculata CS179, a unicellular marine microalga, is rich in long-chain polyunsaturated fatty acids (LCPUFAs). Elongase and desaturase
play a key role in the biosynthesis of PUFAs. A new elongase gene, which encodes 322 amino acids, was identified via RT-PCR and 5′ and 3′ RACE. The sequence of the elongase gene was blast-searched in the NCBI GenBank and showed a similarity
to those of the cryptosporidium. But the NJ-tree revealed that the N. oculata CS179 elongase clustered with those of the microalgae Phaeodactylum tricornutum, Ostreococcus tauri and Thalassiosira pseudonana. 相似文献
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黑鱾血液的生理生化指标研究 总被引：2，自引：0，他引：2
本文采用F-820半自动血细胞分析仪和全自动生化分析仪，对黑纪的血液常规指标及血清中的17项生化指标进行了分析测定，并用Wright’s染色法制作血涂片对白细胞进行分类计数．结果表明：（1）黑纪的血红细胞数量为（2．49±0．47）×10^12个／dm^3；白细胞数量为（1．08±0．21）×10^11个／dm^3；在白细胞中以嗜中性粒细胞数量最多，占52．42％±4．67％，其次为淋巴细胞，占25．72％±2．88％；单核细胞体积最大，占总数的3．27％±0．62％．（2）黑纪红细胞脆性大，抗性差，在0．65％～0．70％的NaCl溶液中开始溶血，在0．50％～0．55％的NaCl溶液中完全溶血．（3）黑纪的血红蛋白（HGB）为109．11±15．72g／dm^3，高于一般鱼类，与其较强的耐低氧生理能力相适应．（4）血清中血糖浓度为4．72±1．35mmol／dm^3，明显低于凶猛肉食性鱼类，与其岛礁性生态习性和杂食性相吻合．（5）雌雄鱼的乳酸脱氢酶分别为332．67±188．48U／dm^3和905．66±257．85U／dm^3，雌鱼显著高于雄鱼，可能与繁殖活动相关． 相似文献
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REN Xueying SUI Zhenghong ZHANG Xuecheng 《中国海洋大学学报(英文版)》2006,5(2):146-150
Glyceraldehyde-3-phosphate dehydrogenase （GAPDH） plays important roles in various cellular processes. A cytosolic GAPDH encoding gene （gpd） of Gracilaria/Gracilariopsis lemaneiformis was cloned and characterized. Deduced amino acid sequence of the enzyme of G. lemaneiformis had high homology with those of seven red algae. The 5＇-untranslated regions of the GAPDHs encoding genes of these red algae varied greatly. GAPDHs of these red algae shared the highly conserved glyceraldehyde 3-phosphate dehydrogenase active site ASCTTNCL. However, such active site of Cyanidium caldarium was different from those of the other six algae at the last two residues （CL to LF）, thus the spatial structure of its GAPDH active center may be different from those of the other six. Phylogenetic analysis indicated that GAPDH of G. lemaneiformis might have undergone an evolution similar to those of Porphyra yezoensis, Chondrus crispus, and Gracilaria verrucosa. C. caldarium had a closer evolutionary relationship with Cyanidioschyzon merolae than with Cyanidium sp. Virtual Northern blot analysis revealed that gpd of G. lemaneiformis expressed constitutively, which suggested that it might be house-keeping and could be adapted as an inner control in gene expression analysis of G. lemaneiformis. 相似文献
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Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro. 相似文献
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休眠与非休眠期中华鳖乳酸脱氢酶同工酶的比较分析 总被引：2，自引：0，他引：2
本文用聚丙烯酰胺凝胶电泳的方法，分析了休眠与非休眠期中华鳖１０种组织的乳酸脱氢酶同工酶，同时测定了休眠期乳酸脱氢酶的总活性。结果表明，某些组织的电泳图谱显示出一定差异；多数组织冬眠期ＬＤＨ同工酶的总活性有所降低。 相似文献