全文获取类型
收费全文 | 189篇 |
免费 | 30篇 |
国内免费 | 59篇 |
专业分类
大气科学 | 1篇 |
地球物理 | 39篇 |
地质学 | 148篇 |
海洋学 | 77篇 |
综合类 | 10篇 |
自然地理 | 3篇 |
出版年
2023年 | 4篇 |
2022年 | 4篇 |
2021年 | 1篇 |
2020年 | 5篇 |
2019年 | 7篇 |
2018年 | 3篇 |
2017年 | 11篇 |
2016年 | 7篇 |
2015年 | 6篇 |
2014年 | 14篇 |
2013年 | 9篇 |
2012年 | 6篇 |
2011年 | 11篇 |
2010年 | 8篇 |
2009年 | 21篇 |
2008年 | 19篇 |
2007年 | 16篇 |
2006年 | 22篇 |
2005年 | 21篇 |
2004年 | 11篇 |
2003年 | 7篇 |
2002年 | 8篇 |
2001年 | 7篇 |
2000年 | 8篇 |
1999年 | 11篇 |
1998年 | 9篇 |
1997年 | 7篇 |
1996年 | 2篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1985年 | 1篇 |
排序方式: 共有278条查询结果,搜索用时 15 毫秒
1.
《Marine pollution bulletin》2014,80(1-2):188-195
A new salt resistant alkaline phosphatase from eggs of the sea urchin Strongylocentrotus intermedius (StAP) has been shown to have a unique property to hydrolyze substrate in seawater without loss of enzymatic activity. The enzyme has pH optimum at 8.0–8.5. Model experiments showed various concentrations of copper, zinc, cadmium and lead added to seawater or a standard buffer mixture to inhibit completely the enzyme activity at the concentrations of 15–150 μg/l. StAP sensitivity to the presence in seawater of metals, pesticides, detergents and oil products appears to be considerably less. Samples of seawater taken from aquatic areas of the Troitsy Bay of the Peter the Great Bay, Japan Sea have been shown to inhibit the enzyme activity; the same was shown for the samples of fresh waters. The phosphatase inhibition assay developed proved to be highly sensitive, technically easy-to use allowing to test a great number of samples. 相似文献
2.
The Engineer Mine epithermal precious metal deposit in British Columbia, Canada, is related to Eocene Sloko-Skukum-Group (SSG) volcanism and, according to previous studies, contains roscoelite in intimate association with electrum. Roscoelite, a vanadian mica, is considered characteristic of low-sulfidation epithermal deposits related to alkaline magmatism. This contradicts the fact that the SSG volcanics are subalkaline. In order to address this ambiguity and to accurately classify the style of epithermal mineralization at the Engineer Mine we conducted detailed petrographic, mineralogical, geochronological, fluid inclusion, and stable isotope studies.The principal ore assemblage of the Engineer Mine epithermal veins precipitated in response to boiling during a hydrothermal event at 49.90 Ma ± 0.25 Ma. During this event electrum, arsenopyrite, pyrite ± chalcopyrite ± sphalerite ± löllingite ± tetrahedrite-group phases ± allargentum ± acanthite ± hessite ± dyscrasite ± stibarsen ± galena and an unidentified Ag-rich phase were deposited in conjunction with amorphous silica, platy and rhombic calcite, K-feldspar, and vanadian illite. Fluid inclusion and stable isotope data suggest that the ore-forming fluid was boiling at ∼220 °C during vein mineralization and had an isotopic composition derived from local meteoric water. Based on these results the Engineer Mine is classified as an epithermal low-sulfidation deposit, which shares similarities with alkaline and subalkaline epithermal low-sulfidation deposits. This is attributed to the fact that the SSG volcanic rocks are borderline subalkaline to alkaline in character and that the sedimentary host rocks are vanadium-bearing. These sedimentary rocks contributed the bulk of the vanadium to the Engineer Mine epithermal system. The presence of roscoelite at the Engineer Mine could not be confirmed during this study. The mica referred to as roscoelite in previous publications instead is vanadian illite. To our knowledge the only alkaline low-sulfidation epithermal precious metal deposit that contains V-mica which exclusively qualifies as true roscoelite is the Porgera deposit, Papua New Guinea. 相似文献
3.
水体细菌碱性磷酸酶及其编码基因研究进展 总被引:1,自引:0,他引:1
水体溶解性反应磷是浮游植物可直接利用的主要磷营养形式,细菌碱性磷酸酶催化的有机磷矿化过程则是维持溶解性反应磷供给的重要途径.通过总结细菌碱性磷酸酶的种类与分布特征,发现主要包括Pho A、Pho X和Pho D 3种类型细菌碱性磷酸酶,其中超过50%的Pho X分布于细菌细胞的外部环境,对溶解性反应磷的循环再生尤为重要.综述水体产碱性磷酸酶细菌群落组成的研究进展,以及影响编码基因表达的环境因素,对比分析产碱性磷酸酶细菌群落鉴定方法的特点及发展趋势.细菌碱性磷酸酶编码基因的研究多关注寡营养海洋生态系统,对于同样存在磷限制问题的富营养化湖泊生态系统鲜有涉及,而构建针对典型富营养化湖泊生态系统的细菌宏基因组数据库,则可为建立淡水生态系统细菌碱性磷酸酶及其编码基因的研究方法奠定基础,有助于认识湖泊水华频发的微生物学驱动机制. 相似文献
4.
盐度对日本鳗鲡生长及非特异性免疫酶活性的影响 总被引:2,自引:0,他引:2
利用实验生态学和酶学方法,在浙江省海洋水产养殖研究所清江试验场研究了盐度对平均体长为25.1±5.6cm的日本鳗鲡(Anguillajaponica)生长及其血清溶茵酶(LZM)、超氧化物歧化酶(SOD)及碱性磷酸酶(AKP)活性的影响,实验持续60d.实验设置了5个盐度梯度(盐度5、10、15、20、25)和对照组(盐度0),每梯度设3个平行组,各组盐度和pH值保持一致.日本鳗鲡血清中免疫酶活力指标:碱性磷酸酶、溶茵酶、超氧化物歧化酶在实验的第7天和第14天测定.实验结果表明,盐度5组相对增重率最高(279.78%±28.37%),其次是盐度10组(278.74%±22.25%),盐度15、20、25组相对增重率分别为234.39%±21.28%、230.28%±26.31%、228.01%±29.29%.对照组相对增重率为233.20%-4-25.18%,盐度5和10组与对照组差异显著(P〈0.05),其余各组与对照组差异不显著.第7天和第14天,盐度10组碱性磷酸酶活性最高,分别为225.05U/dm。和251.11U/dm’,其次是盐度5组,分别为221.76U/dm。和236.01U/dm。,显著高于对照组(P〈0.05).第7天和第14天盐度20、25组溶菌酶活性显著高于对照组(P〈0.01).超氧化物歧化酶随着盐度的升高呈逐步上升趋势.第14天,盐度20、25纽溶菌酶活性仍保持较高水平,SOD回落至对照组水平,而各组AKP较第7天略有上升. 相似文献
5.
新疆东昆仑于沟子地区与铁-稀有多金属成矿有关的碱性花岗岩地球化学、年代学及Hf同位素研究 总被引:2,自引:0,他引:2
东昆仑祁漫塔格地区是近年来发现的具有优越多金属成矿条件的地区,区内中-酸性侵入岩广泛发育且与成矿关系密切。于沟子铁-稀有多金属矿床位于祁漫塔格地区西部,矿床主要由产于钾长花岗岩外接触带矽卡岩内的铁-铜(钼)多金属矿化体及产于花岗岩体内部的铌、铷等稀有元素矿化体组成。锆石LA-MC-ICP-MS定年测得钾长花岗岩U-Pb年龄为210.0±0.6Ma,属晚三叠世末期岩浆活动的产物。岩体主要由条纹长石(60%~65%)、斜长石(10%~15%)、石英(20%~22%)、钠闪石(4%~5%)和少量黑云母(1%~2%)等组成,为典型的碱性花岗岩。地球化学特征上,该岩体具有高硅、高钾、准铝、钙碱性特点,富Nb、Zr、Rb、Th、U,贫Ba、Sr、P、Ti等元素,稀土元素总量较高,富集轻稀土,具强烈的负Eu异常(δEu=0.09~0.26),属于A型花岗岩。锆石εHf(t)值为-6.71~2.25,平均为-1.15,二阶段Hf模式年龄(t DM2)为1102~1674Ma,显示在成岩过程中有地幔物质的参与。综合研究认为,于沟子岩体形成于晚三叠世末后造山板内伸展阶段,该碱性花岗岩的确定,标志着东昆仑祁漫塔格地区在晚三叠世末(212~210Ma)已逐步演化为伸展构造背景下的后造山构造阶段。同时矿床的成矿时代、氧同位素及电子探针结果均显示花岗岩与铁-稀有多金属成矿有关,指示出该类型碱性花岗岩具有良好的铁-稀有多金属成矿潜力。 相似文献
6.
7.
8.
经Tris-HCl缓冲液浸提、硫酸铵分级沉淀盐析、DEAE-Sepharose Fast Flow离子交换层析和Sephadex G-75凝胶过滤层析等步骤,从菲律宾蛤仔内脏团中分离得到碱性磷酸酶,SDS-PAGE显示为单一条带,提纯倍数为14.42,比活力达到131.08U/mg。酶学性质研究表明:该酶亚基分子量约44kD,最适反应温度为45℃,最适pH值为9.0,米氏常数Km为0.098mmol/L,最大反应速度Vmax为1.01μmol/(mL·min)。Ca2+、Mg2+对酶活力表现出显著的激活作用。 相似文献
9.
This study presents a new and first finding of Au-Pb intermetallic compounds in the Inagli Pt–Au placer deposit, the Republic of Sakha (Yakutia), Russia. This is the first time that all three accepted minerals (hunchunite, anyuiite and novodneprite), as well as unnamed Au-Pb intermetallics, corresponding in composition to Au1.5Pb and AuPb, are present together in the same locality. We provide chemical compositions of Au-Pb compounds and host gold particles, describe morphology and relationships between different mineral phases. We also present an unexpected finding of unusual inclusion of Pb-Fe-aluminosilicate associated with K-feldspar and anyuiite in the gold grain. The new data together with data from other occurrences of Au-Pb compounds worldwide were reviewed to discuss type localities, mineralogy, conditions and possible mechanisms of formation of Au-Pb intermetallics and to provide an overview of current knowledge about these uniquely rare but naturally occurring minerals. 相似文献
10.
在滇池福保湾不同区域应用Peeper(渗析膜式)技术,分析了底泥间隙水NH4 -N、Po43--p的垂向分布特征和近表层10cm内底泥的微生物活性(FDA)、碱性磷酸酶活性(APA),并对它们之间的相互关系进行了统计分析.结果表明,NH4 -N和Po43--p浓度自上覆水向下层间隙水呈先升后降趋势,反映它们有自间隙水向上覆水扩散的潜在危害;底泥有机质(Loss-on-Ignion,LOI)、APA和FDA活性也有从表层底泥向下层逐步降低的趋势.在空问分布上,Po43--p浓度变化为河口区>湾心区>西部沿岸区>东部沿岸区,与沉积物中LOI、APA和FDA活性的大小顺序基本相同.间隙水NH4 6-N浓度与表层10cm内底泥的APA和FDA活性具有显著正相关性(α=0.01).Po43--p浓度与底泥APA和FDA活性具有负相关性.但相关系数很低. 相似文献