排序方式： 共有49条查询结果，搜索用时 31 毫秒
1.
2.
采用免培养的16S rDNA梯度凝胶电泳技术（DGGE）对集约化海水网箱养殖川纹笛鲷（Lutjanus sebae）的消化道胃壁、胃内容物、肠壁、肠内容物优势菌群结构进行了比较分析。研究结果显示，川纹笛鲷消化道存在着丰富多样的细菌群落，对DGGE指纹图谱聚类分析表明菌群组成相似度高于55％，其中胃内容物及胃壁细菌组成相似度最高（90％），这可能与摄食饵料在消化道推移有关；而胃壁与肠壁相似度相对最差，可能反应了由于生理环境不同引起的宿主差异性。通过建立川纹笛鲷消化道16S rDNA-DGGE指纹图谱及比较分析，为澄清川纹笛鲷消化道微生物区系奠定了基础。 相似文献
3.
变性梯度凝胶电泳技术是目前研究微生物群落遗传多样性及种群动态性有效手段,已被广泛应用于土壤、活性污泥、生物膜、动物肠道、热泉、湖泊等环境微生物生态学研究。阐述了变性梯度凝胶电泳的原理,分析其在微生物多样性分析方面的优越性及局限性;并着重介绍了PCR-DGGE应用过程中的技术发展,包括Cloning/DGGE,GC-DGGE,Nested PCR-DGGE和DG-DGGE。 相似文献
4.
通过PCR结合变性梯度凝胶电泳(DGGE)技术对从南极长城站附近表层土壤样品中获得16SrDNA序列特征片段V3区序列进行分离。对其中的主要12条DGGE条带进行胶回收,获得的DNA片段经测序以及计算机比对分析发现,它们分别属于β、γ、δ-变形细菌(Proteobac-teria)、噬纤维菌-屈挠杆菌-拟杆菌(Cytophaga-Flexibacter-Bacteroides,CFB)群细菌、放线细菌(Actinobacteria)、蓝细菌属(Cyanobacteria)、酸杆菌属(Acidobacteria)和绿屈挠菌属(Chlo-roflexi)等系统分类群。南极表层土壤样品中的大部分16S rDNA序列与从其他土壤或沉积物样品中直接获得的序列相似性较高(93%-100%)。 相似文献
5.
人工回灌过程中,回灌水的注入使目的含水层地下水环境发生变化,微生物条件也会随之改变,从而影响地下水环境质量及水文地球化学作用。以上海市某人工回灌试验场为例,在分析人工回灌过程中水化学演化特点的基础上,应用DGGE技术对场地回灌过程中地下水中的微生物群落结构变化进行研究,为评价人工回灌对地下水水质安全的影响提供科学的理论依据。结果表明:人工回灌作用使目的含水层地下水中的Eh值及DO质量浓度升高,分别由64.0mV、1.12mg/L升至534.4mV、1.44mg/L;同一位置处微生物群落结构与原始地下水状态的相似性随时间降低;同一时刻距离回灌井越远的监测井的微生物群落结构越接近于原始地下水状态。随着回灌的进行,目的含水层地下水中优势菌属(种)共有7种,其中Rubrivivax gelatinosus和Candidatus Accumulibacter phosphatis clade IIA str.的反硝化能力以及Rhodoferax ferrireducens对Fe3+的还原能力,对地下水水化学组分产生影响。 相似文献
6.
Spatial heterogeneity in a deep artificial lake plankton community revealed by PCR-DGGE fingerprinting 总被引：1，自引：0，他引：1
To explore the spatial heterogeneity of plankton communities in a deep artificial lake(Songhua Lake,China),samples were collected at seven sites. Samples were investigated by denaturing gradient gel electrophoresis(DGGE) analysis of the PCR-amplified 16 S and 18 S r RNA genes and specif ic bands were sequenced. Cluster analysis of the DGGE profiles revealed that all of the samples grouped into two distinct clusters,in accordance with sampling site; while in each cluster,the divergence of sub-clusters correlated with sampling depth. Sequence analysis of selected dominant DGGE bands revealed that most sequenced phylotypes(84%) exhibited ≥97% similarity to the closest sequences in Gen Bank,and were affiliated with ten common freshwater plankton phyla(Proteobacteria,Actinobacteria,Bacteroidetes,Cyanobacteria,Bacillariophyta,Pyrrophyta,Cryptophyta,Ciliophora,Stramenopiles,and R otifera). Several of these groups are also found worldwide,indicating the cosmopolitan distribution of the phylotypes. The relationships between DGGE patterns and environmental factors were analyzed by redundancy analysis(RDA). The results suggested that,total nitrogen,nitrate,nitrite,ammonia,and CODM n concentrations,and water temperature were strongly correlated with the variation in plankton composition. 相似文献
7.
Ships carry ballast water for better stability and to control trim. However, the discharge of ballast water near ports is known to transport invasive species from one coastal area to another. The exchange of ballast water on the high seas is supposed to reduce such invasions of exotic species. In this study, we used denaturing gradient gel electrophoresis (DGGE) to analyze the composition of the bacterial community in ballast water before and after such a mid-ocean exchange, and we also measured total bacterial counts. Our findings confirmed that the ballast water was replaced by the mid-ocean exchange, as indicated by the marked change in the composition of the bacterial community. There was also a significant decrease in bacterial abundance after the mid-ocean exchange. Finally, our findings support the incubation hypothesis, because the composition of the bacterial communities changed over time within the same ballast water. 相似文献
8.
9.
Physicochemical and microbiological characterization of anoxic sediments taken from seven highly polluted sites of the Marmara Sea was carried out. The 16S rRNA based microbial community structure analyses were performed using domain-specific PCR followed by denaturant gradient gel electrophoresis (DGGE) and sequencing of characteristic bands. The results showed that the microbial communities in these sediments were diverse and evenly distributed. Relating the prokaryotic and geochemical variables through statistical tools revealed that the microbial diversity in the sediments significantly related to depth, and S, Mn and Fe content of the sediments. Fermentative bacteria, denitrifying bacteria and hydrogenotrophic methanogens were dominant whereas sulfate reducing bacteria were absent in the DGGE patterns. This unusual microbial community structure implied that the newly discovered anaerobic methane oxidation coupled to denitrification process may occur in these subseafloor environments. 相似文献
10.