中国沿海常见蜑螺科贝类的DNA条形码   总被引：1，自引：0，他引：1  

张晓洁  孔令锋  李琪 《海洋与湖沼》2018,49(3):614-623

DNA条形码不仅为物种鉴定提供了有效方法,而且也有助于分类学和生物多样性研究。本研究旨在探讨将COI和16S rRNA基因序列应用于中国沿海蜑螺科贝类物种鉴定的可行性,获得了该科3属7种贝类61个个体的COI和16S rRNA基因序列。基于COI基因序列的种内遗传距离为0.00—1.29%,平均为0.67%;属内种间遗传距离为4.62%—19.25%,平均为13.02%;基于16S rRNA基因序列的种内遗传距离为0.00%—0.48%,平均为0.23%;属内种间遗传距离为2.47%—8.48%,平均为6.37%。两种基因序列在所研究的蜑螺中,种内遗传差异均小于种间遗传差异,存在明显的条形码间隙,所有物种在系统发生树上都表现为独立的单系群。结果表明,线粒体COI和16S rRNA基因序列可以作为DNA条形码标准基因对蜑螺科贝类进行有效地物种鉴定。  相似文献   

基于COⅠ基因序列的长腹剑水蚤系统进化关系     

王兴霞  徐磊  王亮根  陈骁  郑媛媛  王雪辉  杜飞雁 《海洋学报》2018,40(6):92-103

长腹剑水蚤属（Oithona）是广泛分布于海洋近岸和外海海域的中小型桡足类中最为丰富的类群之一，由于个体小且形态差异微小，通过传统的形态学分类法对其进行准确鉴定难度较大。本文对南海分布的长腹剑水蚤属内的5个种，即瘦长腹剑水蚤（O.tenuis）、羽长腹剑水蚤（O.plumifera）、刺长腹剑水蚤（O.setigera）、伪长腹剑水蚤（O.fallax）和长刺长腹剑水蚤（O.longispina）线粒体COⅠ基因序列以及DNA数据库中长腹剑水蚤属其他地区种类COⅠ基因序列进行比较分析，使用ABGD （Automatic Barcode Gap Discovery）和GMYC （Generalized Mixed Yule Coalescent）模型进行物种界定，分析种间种内遗传距离，并构建系统进化关系。结果显示ABGD和GMYC模型均可以很好地对长腹剑水蚤进行种类划分；种内遗传距离为0.0%~1.6%，种间遗传距离为17.7%~44.5%（Kimura 2-parameter双参数模型），表明种间出现较高的分化；贝叶斯系统树和最大似然树进化树结果均表明，简长腹剑水蚤（O.simplex）与其他种类相距较远，羽长腹剑水蚤和拟长腹剑水蚤（O.similis）中存在隐种的分化，分别是我国南海海域和地中海的羽长腹剑水蚤以及朝鲜海峡和北海的拟长腹剑水蚤，种间遗传距离分别为18.6%、22.9%。  相似文献   

虾夷扇贝TET基因在配子发生和早期发育中的表达模式     

李仰平  张玲玲  李若佼  刘甜  郭振义  李婉茹  包振民 《中国海洋大学学报(自然科学版)》2020,50(4):54-62

DNA甲基化参与调节动物配子发生和胚胎发育过程,TET基因负责DNA主动去甲基化,在基因印迹去除和细胞全能性获得中起重要作用。为了解海洋贝类配子发生和胚胎发育过程中DNA去甲基化如何发生,本研究以虾夷扇贝为研究对象,鉴定了其TET基因,并分析了该基因在性腺和胚胎幼虫发育中的表达变化。结果表明,虾夷扇贝基因组中含有1个TET基因(PyTET),该基因长39127 bp,包含10个外显子,编码1592个氨基酸,其蛋白具有完整的2OGFeDO超家族的加氧酶结构域。在性腺发育过程中,PyTET基因表达峰值出现在休止期精巢和增殖期卵巢,原位杂交结果显示其在精原细胞、精母细胞中均有表达,在卵母细胞中的表达明显高于卵原细胞;在早期发育过程中,其峰值出现在囊胚期。以上结果提示虾夷扇贝配子发生和胚胎发育过程中均发生了DNA主动去甲基化,这将有助于系统了解表观调控在贝类发育中的作用。  相似文献   

Sensitive and rapid detection of two toxic microalgae Alexandrium by loop-mediated isothermal amplification     

ZHANG Fengying  SHI Yanhong  JIANG Keji  XU Zhaoli  MA Lingbo 《海洋学报(英文版)》2018,37(2):139-146

A loop-mediated isothermal amplification (LAMP) assay was designed and evaluated for rapid detection of the toxic microalgae Alexandrium catenella and A. minutum, which can produce paralytic shellfish poisoning (PSP). Two sets of four specific primers targeting these two species were derived from the sequence of internal transcribed spacer (ITS) of ribosomal DNA. The method worked well in less than an hour under isothermal conditions of 65℃. LAMP specificity was validated in closely related algae as a comparison, suggesting the strict specificity of the LAMP primers. Two visual inspection approaches were feasible to interpret the positive or negative results. The detection limits of A. catenella and A. minutum samples using the LAMP assay were found to be 5.6 and 4.5 pg DNA, respectively. The sensitivity of this LAMP assay was 10 or 100-fold higher than Polymerase Chain Reaction (PCR) method in detecting the two microalgae. These characteristics of species specificity, sensitivity, and rapidity suggest that this method has the potentiality in the monitoring of red tide caused by A. catenella and A. minutum.  相似文献   

基于DNA条形码技术的乌苏里江中下游漂流性鱼卵鉴定   总被引：1，自引：0，他引：1  

唐富江  王继隆  那荣滨  刘伟  鲁翠云 《湿地科学》2020,18(6):653-659

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Morphological and molecular evidence supports the first occurrence of two fishes,Siganus sutor(Valenciennes, 1835)and Seriolina nigrofasciata(Rüppell, 1829)(Actinopterygii:Perciformes), from marine waters of Odisha coast,Bay of Bengal,India     

Barik Tapan K.  Swain Surya N.  Sahu Bijayalaxmi  Tripathy Bibarani  Acharya Usha R. 《海洋学报(英文版)》2020,39(6):26-35

Marine ecosystems provide a wide variety of diverse habitats that frequently promote migration and ecological adaptation. The extent to which the geographic distribution of marine organism has reshaped by human activities remains underappreciated. The limitations intrinsic to morphology-based identification systems have engendered an urgent need for reliable genetic methods that enable the unequivocal recognition of fish species,particularly those that are prone to overexploitation and/or market substitution. In the present study, however, an attempt has been taken to identify two locally adapted fish species, Siganus sutor(Valenciennes, 1835) and Seriolina nigrofasciata(Rüppell, 1829) of order Perciformes, which happens to be the first record in Odisha coast,Bay of Bengal. The diagnostic characteristics of Siganus sutor are: dorsal fin XIII-10, anal fin VII-9, pectoral fin 15,pelvic fin II-3, while that of Seriolina nigrofasciata dorsal fin VI-I-35, anal fin I-17, pectoral fin 16, pelvic fin 5. All COI barcodes generated in this study were matched with reference sequences of expected species, according to morphological identification. Bayesian and likelihood phylogenetic trees were drawn based on DNA barcodes and all the specimens clustered in agreement with their taxonomic classification at the species level. The phylogeographic studies based on haplotype network and migration rates suggest that both the species were not panmitic and the high-frequency population distribution indicates successful migration. The result of this study provides an important validation of the use of DNA barcode sequences for monitoring species diversity and changes within a complex marine ecosystem.  相似文献   

高分二号卫星影像融合方法比较及效果优化研究   总被引：1，自引：0，他引：1  

汤耶磊  王鸿燕  刘荣 《测绘与空间地理信息》2018,(2):43-48

针对如何使高分二号卫星融合后影像达到最佳视觉与应用效果还没有达成统一意见的问题,本文采用Gram-Schmidt(G-S)、高通滤波法(HPF)、最邻近扩散算法(NND)、超分辨率贝叶斯法及改进算法(Pansharp及Pansharp2)等5种目前主流的遥感影像融合算法,对高分二号卫星全色及多光谱影像数据开展融合实验,梳理了影像融合质量评价体系,同时对融合结果进行通道重组实验及优化。结果表明:NND、HPF、Pansharp融合算法比较适合高分二号卫星影像,将20%近红外波段加入到绿波段时,各融合算法影像视觉效果都得到增强,其中NND融合算法光谱性良好,HPF融合算法清晰度最好,Pansharp融合算法效果稳定,可作为后备方法,实验结果将为后续高分系列卫星影像处理提供支持。  相似文献   

北京西北城区一次重污染过程中PM₁₀的氧化损伤能力研究     

席春秀  邵龙义  吴凡  常玲利  李杰 《岩石矿物学杂志》2018,37(3):492-496

为评估大气重污染期间北京市可吸入颗粒物(PM_(10))的毒性,采集2016年3月份一次大气重污染过程中北京市大气PM_(10)样品,应用质粒DNA损伤评价法来研究其氧化损伤能力。结果表明,雾霾期间PM_(10)对DNA的损伤率高于雾霾逐渐消退时期,远高于雾霾前期清洁天,颗粒物对DNA损伤率随剂量的增加而增加。雾霾前后PM_(10)水溶样品的平均TD_(20)值表现为雾霾前清洁天(788.01μg/mL)雾霾消退后期(470.40μg/mL)雾霾期间(55.78μg/mL),说明氧化能力雾霾期间雾霾消退后期雾霾前清洁天。另外通过雾霾前后数据对比得到,暴露毒性指数TI大小顺序为雾霾期间(13 245.06)雾霾消退后期(1 658.87)雾霾前清洁天(254.08),说明雾霾期间PM_(10)对人体危害更大。  相似文献   

环境DNA——洞穴生物研究中的新技术     

白洁  邢迎春  高婉茹  赵亚辉 《中国岩溶》2021,40(6):1014-1020

重组性迁移在主题式教学中的应用研究 ——以高中地理教学为例     

梁梅青  乐淮辉 《地理教学》2019,(9)

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