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1.
【目的】探索波吉卵囊藻胞外滤液对铜绿微囊藻的抑制机理。【方法】采用Illumina平台,对使用BG11培养基(对照组)和波吉卵囊藻胞外滤液(实验组)培养的铜绿微囊藻(Microcystis aeruginosa)进行转录组测序,筛选差异表达基因并进行通路富集分析,并对部分差异表达基因进行qRT-PCR验证。【结果】与对照组相比,波吉卵囊藻胞外滤液处理后的铜绿微囊藻组中筛选1483个表达差异显著的基因,其中上调表达基因788个,下调表达基因695个。GO功能分析将差异基因划分为35个子类别,包括催化活性、结合、代谢过程、细胞过程、膜、膜部分等。KEGG通路分析中,所有差异基因聚集在108个通路,其中84个差异基因注释在代谢功能类别中,而氧化磷酸化通路富集最显著。氧化磷酸化通路中,相关基因表达以上调为主(26个DEGs,20个上调,6个下调)。qRT-PCR验证结果与转录组测序结果的表达变化趋势一致。【结论】波吉卵囊藻滤液引起铜绿微囊藻氧化磷酸化途径上基因表达上调,ATP合成效率提高。  相似文献   
2.
根据2005—2017年卫星遥感反演的太湖蓝藻水华信息,利用区域气象观测数据分析各类气象因子对太湖蓝藻深水华形成的影响,结果表明:①在日平均气温为0~35℃区间内会出现蓝藻聚集现象,其中最适宜气温区间为15.1~35℃,该区间内累计出现蓝藻水华的面积占比达90%、出现大面积蓝藻水华占比达93%;②在卫星观测到蓝藻前6h,平均风速为0.2~4.8m/s区间内能观测到蓝藻水华,其中最适宜的平均风速区间为0.5~3.4m/s,该区间内蓝藻水华累计出现次数占比达94.7%,大面积蓝藻水华主要出现在平均风速2.0m/s的情况下,占比89%;③降水总体上呈负效应,但在观测到有大面积蓝藻水华的情况下,前24h有小雨(10mm)的情形与总降水次数之比达88%,说明小雨的适度扰动有利于形成大面积蓝藻水华;④日照充足有利于蓝藻生长,但并非蓝藻水华形成的必要条件。在此基础上建立的多元回归综合气象指数模型,拟合结果较好,通过了0.001的显著性检验。  相似文献   
3.
在巢湖西北半湖近岸带设置大型围隔研究秋季连续打捞蓝藻对湖泊温室气体通量的影响,应用YL-1000型大型仿生式水面蓝藻清除设备进行原位打捞蓝藻,通过便携式温室气体分析仪-静态箱法对大型围隔内水-气界面CH4、CO2通量特征及其影响因素进行观测.结果表明:对比未打捞区,蓝藻连续打捞下打捞区水体中叶绿素a(Chl.a)、悬浮物(SS)浓度不断下降,两者削减率分别为72%、85%,Chl.a、SS浓度分别下降到29.6±2.5 μg/L、12.5±1.2 mg/L,打捞对围隔内颗粒态物质去除效果十分明显;打捞过程中水体溶解性有机物(DOM)中微生物代谢类腐殖质(C1)、类蛋白(C3)显著下降趋势,打捞区C1、C3组分(0.18±0.02、0.06±0.01 RU)强度明显低于未打捞区(0.26±0.05、0.12±0.03 RU),打捞能有效控制藻源性溶解性有机质释放.同时,打捞区水-气界面CH4通量呈显著下降趋势,未打捞区CH4通量平均值(17.473±1.514 nmol/(m2·s))为打捞区(7.004±4.163 nmol/(m2·s))近2倍,CH4通量与Chl.a、C1、C3组分均呈显著正相关,水体中藻源性溶解态有机质对CH4通量具有促进作用;打捞区CO2释放通量呈显著上升趋势,打捞区CO2吸收通量(-0.200±0.069 μmol/(m2·s))明显低于未打捞区(-0.344±0.017 μmol/(m2·s)),CO2通量与Chl.a、温度均呈显著负相关.秋季打捞对CH4、CO2综合日平均通量减排量值为0.275±0.076 mol/(m2·d)(以CO2当量计).研究结果揭示了巢湖秋季连续打捞蓝藻过程对水-气界面温室气体具有显著减排作用,且能在一定程度上减缓蓝藻水华与湖泊富营养化、气候变暖之间的恶性循环,为湖泊碳循环和蓝藻水华灾害防控提供科学数据支撑和理论参考.  相似文献   
4.
Reservoirs of lowland floodplain rivers with eutrophic backgrounds cause variations in the hydrological and hydraulic conditions of estuaries and low-dam reservoir areas, which can promote planktonic algae to proliferate and algal bloom outbreaks. Understanding the ecological effects of variations in hydrological and hydraulic processes in lowland rivers is important for algal bloom control. In this study, the middle and lower reaches of the Han River, China, a typical regulated lowland river with a eutrophic background, are selected. Based on the effect of hydrological and hydraulic variability on algal blooms, a hydrological management strategy for river algal bloom control is proposed. The results showed that (a) differences in river morphology and background nutrient levels cause significant differences in the critical threshold flow velocities for algal bloom outbreaks between natural river and low-dam reservoir sections; there is no uniform threshold flow velocity for algal bloom control. (b) There are significant differences in the river hydrological/hydraulic conditions between years with and without algal blooms. The average river flow, water level and velocity in years with algal blooms are significantly lower than those in years without algal blooms. (c) For different river sections where algal blooms occur and to meet the threshold flow velocities, the joint operation of cascade reservoirs and diversion projects is an effective method to prevent and control algal blooms in regulated lowland rivers. This study is expected to deepen our understanding of the ecological significance of special hydrological processes and guide algal bloom management in regulated lowland rivers.  相似文献   
5.
城市淡水系统的富营养化风险是世界范围内普遍关注的问题。多种物理、生物和化学技术手段被应用到富营养化湖库的治理当中,以期抑制水体的富营养化程度和藻类生物量。经证实,在未有效降低营养盐来源的情况下,这些手段的效果有限。而在发展中国家,控制营养盐来源可能需要花费数十年的时间。本研究旨在分析某一高营养盐负荷的沿海水库的富营养化和藻类水华风险,以期确认通过水利调度来抑制水库富营养化状态的可行性。该案例水库为位于长江口的青草沙水库。该水库2009至2012年期间的库内五个点位的水质数据被用于进行案例分析。水质指标包括水温、透明度、溶解氧、总氮、总磷和浮游植物叶绿素a。该水库的建设期为2009年4月至2010年10月,期间水库未曾与长江口发生水体交换。该水库的试运营期为2010年10月至2011年1月,正式运营期为2011年1月至今。在运营期间,库内与长江口的水体交换逐步上升。综合营养状态指数(TLI)被用于评估该水库的营养状态变化情况,该指数是通过数个代表性水质指标计算得到。库区的TLI指数峰值在2009年夏季可达51,在2011年夏季可达55,超过TLI指数的富营养化阈值50。TLI的谷值32出现在2010年的夏季。水质观测期的其他时段的TLI指数均可保持在50以下。以上分析结果表明:水库在2009年和2011年夏季由于过量的营养盐负荷和藻类水华迅速恶化到富营养化状态。水库在2010年和2012年均未出现富营养化状态和藻类水华,这是由于2010年期间水库缺少营养盐输入,2012年期间水库调度充分地置换了库区水体。库区水质指标的时空变化均通过文中的观测资料和数据分析进行展示。经分析表明,通过潮汐涨落来充分置换库区水体的水库调度手段是一个极为经济有效的抑制高营养盐水体富营养化和藻类水华的工程手段。  相似文献   
6.
Laboratory experiments on the New Zealand freshwater mussel Echyridella menziesii were used to investigate the short-term effects (7–8 days) of food type on rates of biodeposition and benthic substrate respiration. Post-feeding biodeposition rates ranged from 0.34 to 1.52?mg?g?1?h?1 (mean?=?0.50?mg g?1?h?1) and were unaffected by the addition of toxin-producing Microcystis. Addition of suspended sediment (30?mg?L?1) visibly altered substrate composition, and increased total and inorganic biodeposit production rates by 24–33% compared to mussels fed commercial phytoplankton stock. Biodeposition rates of mussels in lake bed substrates were 38% higher than those in silica sand for identical feeding regimes, suggesting that a significant proportion of material produced in this experiment could have been derived from feeding on organic matter in the lake bed sediments. Respiration rates were higher in treatments with Microcystis but were unaffected by the presence of mussels. This laboratory study suggests that biodeposition by E. menziesii is resilient to short-term exposure to Microcystis, and highlights the ability of mussels to alter benthic substrate composition by incorporating suspended sediment into substrates.  相似文献   
7.
Microbioerosion rates and microbioeroder community structure were studied in four Kenyan protected coral-reef lagoons using shell fragments of Tridacna giant clams to determine their response to the influence of terrestrial run-off. Fourteen different microbioeroder traces from seven cyanobacteria, three green algae and four fungi species were identified. The river discharge-impacted reef and ‘pristine’ reef showed similar composition but higher microbioeroder abundance and total cyanobacteria- and chlorophyte-bioeroded areas when compared with the other study reefs. Cyanobacteria dominated during the north-east monsoon (NEM) relative to the south-east monsoon (SEM) season, with algae and cyanobacteria being major microbioeroders in the river-impacted and pristine reefs. The rate of microbioerosion varied between 4.3 g CaCO3 m?2 y?1 (SEM) and 134.7 g CaCO3 m?2 y?1 (NEM), and was highest in the river-impacted reef (127.6 g CaCO3 m?2 y?1), which was almost double that in the pristine reef (69.5 g CaCO3 m?2 y?1) and the mangrove-fringed reef (56.2 g CaCO3 m?2 y?1). The microbioerosion rates measured in this study may not be high enough to cause concern with regard to the health and net carbonate production of Kenya’s coral reefs. Nevertheless, predicted increases in the frequency and severity of stresses related to global climate change (e.g. increased sea surface temperature, acidification), as well as interactions with local disturbances and their influence on bioerosion, may be increasingly important in the future.  相似文献   
8.
Periodical algal blooms result in deposition and release of phosphorus (P) from the sediment into the water. Therefore, during seasonal changes when algal particles begin to settle to the bottom, understanding the behavior and distribution characteristics of the P in sediment is the most important key to manage the water quality of the Saemangeum Reservoir. In this study, the variation of water quality and sediment composition including chlorophyll-a (Chl-a) and P was investigated to determine the interaction between water and sediment. The study focused primarily on algal particle sedimentation that affects the P release and mineralization of sediment. The Chl-a concentration in water showed a sharp decline in October when the algae began to die in the fall, and afterward the concentration of chemical oxygen demand (COD) and total P (TP) in the sediment increased due to the sedimentation of decaying algal particles in November. During the same period of time, the readily bio-available P (RAP) in the sediment showed a drastic increase in the upper region where the Chl-a concentration of water was high. In sequence, the high RAP zone shifted from the upper region to the lower region in the early winter. The RAP shift was considered to be derived from the physical flow of the overlying water from which the decomposing algae settled on the surface of the sediment. The Saemangeum Reservoir was constructed recently; therefore, all the types of inorganic P fractions except soluble reactive phosphorus (SRP) that exist on the bottom surface of the lake and the marsh's sediment layer were not sufficient to significantly influence the overlying water. On the other hand, the released P from the algae was distinct and sensitive to the seasonal change. In conclusion, the algal particle sedimentation was important to control eutrophication rather than P release from the mineralized inorganic P of the sediment surface layer in the Seamangeum Reservoir.  相似文献   
9.
为了揭示磷(P)营养缺乏对蓝藻释放挥发性有机化合物(VOCs)的影响及其对其他藻类的化感作用,以形成蓝藻水华的主要种类铜绿微囊藻(Microcystis aeruginosa)为材料,在无P培养条件下对其释放的VOCs进行分析,同时测定VOCs对莱茵衣藻(Chlamydomonas reinhardtii)生长、光合色素含量和光合性能的影响.结果表明,采用无P培养基培养铜绿微囊藻24 h后,其释放的VOCs种类和含量均明显增加,与标准培养基培养相比,VOCs总释放量增加了73.4%,并出现7种新化合物.将铜绿微囊藻释放的VOCs通入莱茵衣藻溶液中,在标准培养基中铜绿微囊藻释放的VOCs对莱茵衣藻生长无显著影响,而无P条件下释放的VOCs则明显抑制莱茵衣藻生长,其响应指数(RI)为-0.25.此外,莱茵衣藻光合色素含量、光系统II(PSII)最大光化学量子产量(Fv/Fm)、有效光化学量子产量[Y(II)]、光化学淬灭系数(q P)和光合电子传递速率(ETR)也明显降低,而非光化学淬灭系数(NPQ)则明显升高,其RI为0.26.由此可见,蓝藻在富营养化水体中大量繁殖以及P自身沉降特性导致的P缺乏会促进蓝藻释放VOCs,同时这些VOCs在保持蓝藻营养竞争优势和水体藻类多样性减少中具有化感抑制作用.  相似文献   
10.
方婷轩  马增岭 《湖泊科学》2018,30(3):732-740
浮游植物间的交互化感作用被认为是自然水体中浮游生物群落演替及优势种转换的主要因素之一.铜绿微囊藻(Microcystis aeruginosa)是富营养化湖泊中的常见蓝藻,其产毒品系相较非产毒品系具有较高的竞争优势,其产生的主要次生代谢产物微囊藻毒素-LR(MC-LR)通常被认为是重要的化感物质.但是到目前为止其在水生生态系中的生物学功能尚不明确.为探究并区分MC-LR及其他次生代谢产物对浮游植物的化感效应,本文研究了MC-LR与高浓度普通小球藻(Chlorella vulgaris)生长及有效量子产率的剂量—效应关系;比较了能够产生显著抑制效应的MC-LR、能够产生同等浓度MC-LR的产毒品系铜绿微囊藻滤液以及细胞粗提液对小球藻生长及有效量子产率的抑制效应.结果显示,MC-LR能够对小球藻生长及有效量子产率产生抑制作用,并且抑制率随着MC-LR浓度的增加而增大.在用200μg/L及以上浓度的MC-LR感染24 h后,叶绿素a浓度为1500μg/L的小球藻的生长与有效量子产率均比对照组显著降低.能够产生相同浓度MC-LR的产毒品系铜绿微囊藻细胞滤液对其没有产生显著影响,而其细胞粗提液却能够产生更强的抑制效应.与MCLR处理组相比,粗提液处理组小球藻的生长及有效量子产率分别降低了14%和3%.以上结果表明,MC-LR对普通小球藻的化感作用具有剂量依赖性;MC-LR之外的其他次生代谢物也能够产生抑制效应.  相似文献   
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