排序方式： 共有4条查询结果，搜索用时 31 毫秒
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以天蓝喇叭虫(Stentor coeruleus)为研究对象,探索了单细胞单基因PCR扩增及单细胞全基因组PCR扩增技术在原生动物中的应用.经过不断探索和优化条件后,试验取得了理想的结果.在40例单细胞单基因(SSU rDNA基因全序列)PCR的一次性扩增中,新鲜细胞和经过中性红染色的细胞都获得了100%的成功率,室温下酒精(95%)保存一周的细胞获得了82.5%的成功率.在单细胞全基因组PCR扩增中,采用高效高保真的phi29 DNA聚合酶结合随机引物(Random Primer)进行扩增,获得了丰富且质量较高的PCR产物.以全基因组扩增产物(稀释10倍)对四个常用的基因位点(TEF1、SSU rRNA、18S-ITS1-5.8S、a-tubulin)进行扩增,均成功获得相应的基因片断. 相似文献
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This study showed first results on the geographical distribution of mixotrophic ciliates of the genus Stentor in the Chilean North Patagonian Lake District. Thirteen deep North Patagonian lakes were sampled during 2004 and 2005, and important ecological parameters as nutrients, light climate, chlorophyll a and all plankton groups were analysed. The data were evaluated using correlation, cluster and discriminant function analyses.In four of 13 lakes, Stentor was an important constituent of the plankton community and contributed significantly to the total zooplankton biomass. It coinhabited lakes with calanoid copepods (Boeckella, Tumeodiaptomus) and small cladocerans (Eubosmina, Ceriodaphnia). Stentor was negatively correlated with cyclopoid copepods and large cladocerans. Cyclopoid copepods were a very good predictor to discriminate between lakes with and without Stentor. It is suggested that cyclopoid copepods have top-down impact on Stentor. 相似文献
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利用筛选的12条10 bp的随机引物对采自武汉市东湖(3个样点)、南湖(3个样点)、月湖(1个样点)和关桥(1个样点)四个水体的天蓝喇叭虫(Stentor coeruleus)种群进行了随机扩增多态DNA(RAPD)研究,所得清晰条带显示不同样点样本之间存在着一定的变异,其遗传距离在0.076-0.416之间.用Rapdistance 1.04构建聚类图并探讨不同样点之间的遗传距离远近.结果显示南湖的3样点的遗传距离较近,在聚类图上聚成一枝,应该为同一个种群;而东湖的3个样点可能是由于地理隔离原因,在聚类图上有2个样点聚成一枝,而另1个样点被其他样点所分隔,不属于同一个种群.这种现象的发现从实例上支持了生物地理学关于物种形成的离散假说.更有意义的是本研究可启动水体微生物种群分化和物种形成及过渡的研究,并期望促进种群生态学方面的研究和谱系生物地理学及离散分化生物地理学的发展. 相似文献
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The rhodophyte Compsopogon coeruleus (Balbis ex C. Agardh) Montagne is newly recorded from Fiji, South Pacific. Plants occur in dense masses in the Wainivesi River in the province of Tailevu. Local villagers collect the plants and use them to cook a “pudding” with tinned fish, very similar to “puddings” prepared by coastal Fijians using marine species such as Gracilaria and Hypnea. This is the first documented account of the use of Compsopogon as a gelling agent in the preparation of human food. 相似文献
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