基于转录组测序对两种氮素营养条件下多形微眼藻氮代谢途径的解析     

李迎  米铁柱  乔玲  甄毓 《海洋与湖沼》2019,50(6):1241-1251

探究微藻的氮代谢通路对了解其对不同氮源利用的分子机制具有重要意义。本研究利用Illumina高通量测序技术对两种氮素营养条件下(硝酸氮和尿素)多形微眼藻的转录组进行分析,通过基因功能注释及数字基因表达谱分析,研究了多形微眼藻细胞内氮代谢的调控机制。结果检测出15种参与氮代谢的酶,对应76个编码基因,构建了多形微眼藻的氮代谢通路图。其中10个酶编码基因在两种不同氮素营养条件下存在差异表达,最显著的是谷氨酸合成酶、谷氨酸脱氢酶和谷氨酰胺合成酶相关基因。有机氮源(尿素)实验组中,多形微眼藻细胞内的硝酸盐还原酶、亚硝酸盐还原酶等基因的差异表达明显高于无机氮源(硝酸钠)实验组,表明当环境中的氮源为尿素时,会对多形微眼藻细胞内硝酸盐的转化和利用有一定影响。本研究初步阐述了硝酸盐、尿素的吸收转运对多形微眼藻细胞内氮代谢的影响机制,可为硅藻在不同氮素营养条件下的吸收利用机制及氮代谢响应研究提供依据。  相似文献   

A comparison of the effectiveness of the roots of two grass species in reducing soil erosion on alluvial fans in south-east China     

Fangshi Jiang  Kaiwen He  Jiahui Lin  Hui Li  Zhenzhi Zhan  Jinshi Lin  Hongli Ge  Ming Kuang Wang  Yanhe Huang 《水文研究》2020,34(1):96-110

The effects of root systems on soil detachment by overland flow are closely related to vegetation types. The objective of this study was to quantify the effects of two gramineous roots (Paspalum mandiocanum with shallow roots and Pennisetum giganteum with deep roots) on soil detachment capacity, rill erodibility, and critical shear stress on alluvial fans of benggang in south-east China. A 4-m-long and 0.12-m-wide flume was used. Slope steepness ranged from 9% to 27%, and unit flow discharge ranged from 1.39 × 10⁻³ to 4.19 × 10⁻³ m² s⁻¹. The mean detachment capacities of P. mandiocanum and P. giganteum lands were 18% and 38% lower than that of bare land, respectively, and the effects of root on reducing soil detachment were mainly reflected in the 0- to 5-cm soil layer. The most important factors in characterizing soil detachment capacity were root length density and soil cohesion, and soil detachment capacity of the two grass lands could be estimated using flow shear stress, soil cohesion, and root length density (NSE = 0.90). With the increase in soil depth, rill erodibility increased, whereas shear stress decreased. The mean rill erodibilities of P. mandiocanum and P. giganteum lands were 81% and 61% as much as that of bare land, respectively. Additionally, rill erodibilities of the two grass lands could be estimated as an exponential function by root length density and soil cohesion (NSE = 0.88). The mean critical shear stress of P. mandiocanum and P. giganteum lands was 1.29 and 1.39 times that of bare land, respectively, and it could be estimated with a linear function by root length density (NSE = 0.76). This study demonstrated that planting of the two grasses P. mandiocanum and P. giganteum could effectively reduce soil detachment and enhance soil resistance to erosion on alluvial fans, with the deep roots of P. giganteum being more effective than the shallow roots of P. mandiocanum. The results are helpful for understanding the influencing mechanism of root systems on soil detachment process.  相似文献   

激光跟踪仪在核电站主管道安装中的应用     

郭建兵 《北京测绘》2020,(1):123-125

目前,核电站的主管道焊接越来越多的采用了自动焊技术,这有利于提高焊接质量效率,还可以压缩建设成本。然而,主管道自动焊对焊缝的限差较现有工艺要求更高,高精度的测量是实现主管道自动焊的必要条件之一。本文针对主管道自动焊管口组队高精度的技术要求,提出采用激光跟踪仪对主管道自动焊相关的设备进行更为精密的三维工业测量,通过理论技术评定测量精度,然后选择测量仪器和编制具体的测量方案,最终保证了主管道自动焊精确组对的实现。  相似文献   

The GEOMON network of Czech catchments provides long-term insights into altered forest biogeochemistry: From acid atmospheric deposition to climate change     

Filip Oulehle  Milan Fischer  Jakub Hruška  Tomáš Chuman  Pavel Krám  Tomáš Navrátil  Miroslav Tesař  Miroslav Trnka 《水文研究》2021,35(5):e14204

In 1994, a network of small catchments (GEOMON) was established in the Czech Republic to determine input–output element fluxes in semi-natural forest ecosystems recovering from anthropogenic acidification. The network consists from 16 catchments and the primary observations of elements fluxes were complemented by monitoring of biomass stock, element pools in soil and vegetation, and the main water balance components. Over last three decades, reductions of SO₂, NO_x and NH₃ emissions were followed by sulphur (S) and nitrogen (N) deposition reductions of 75% and 30%, respectively. Steeper declines of strong acid anion concentrations compared to cations (Ca, Mg, Na, K, NH₄) in precipitation resulted in precipitation pH increase from 4.5 to 5.2 in bulk precipitation and from 4.0 to 5.1 in spruce throughfall. Stream chemistry responded to changes in deposition: S leaching declined. However at majority of catchments soils acted as a net source of S to runoff, delaying recovery. Stream pH increased at acidic streams (pH < 6) and aluminium concentration decreased. Stream nitrate (NO₃) concentration declined by 60%, considerably more than N deposition. Stream NO₃ concentration was tightly positively related to stream total dissolved nitrogen to total phosphorus (P) ratio, suggesting the role of P availability on N retention. Trends in dissolved organic carbon fluxes responded to both acidification recovery and to runoff temporal variation. An exceptional drought occurred between 2014 and 2019. Over this recent period, streamflow decreased by ≈ 40% on average compared to 1990s, due to the increases of soil evaporation and vegetation transpiration by ≈ 30% and declines in precipitation by ≈ 15% on average across the elevational gradient. Sharp decreases of stream runoff at catchments <650 m a.s.l. corresponded to areas of recent forest decline caused by bark beetle infestation on drought stressed spruce forests. Understanding of the interactions among legacies of acidification and eutrophication, drought effects on the water cycle and forest disturbance dynamics is requisite for effective management of forested ecosystems under anthropogenic influence.  相似文献   

波浪作用下沉积物中氮、磷释放速率的试验研究     

张严严  房文艳  许国辉  任宇鹏  许兴北 《中国海洋大学学报(自然科学版)》2020,50(4):102-110

湖泊或海湾中的沉积物吸附氮(N)、磷(P)等元素所形成的沉积层成为水体富营养化的內源。在波浪作用下,尤其是强浪作用下,沉积物中的N、P会通过内源释放作用大量进入水体中。本文在自制的U型水槽中开展实验,采用不同水头差来模拟波浪的循环荷载作用,研究了沉积层中的N、P在静置固结状态(Ⅰ)、加波未液化状态(Ⅱ)、加波液化状态(Ⅲ)下的释放规律,并给出该试验条件下的沉积物释放速率的拟合方程。试验结果表明:沉积物中总氮(TN)和溶解性总氮(TDN)的释放速率会随着水动力作用的增强而增加;总磷(TP)、溶解性总磷(TDP)和活性磷酸盐(SRP)的释放速率也随着水动力作用增强而增加,但水体中悬浮物(SS)含量过高会限制其释放速率。  相似文献   

Land Resource Carrying Capacity in Xilin Gol Grassland Transects: A Perspective on Food Nutritional Demand     

YANG Wanni  ZHEN Lin  LUO Qi 《资源与生态学报(英文版)》2019,10(6):614-620

Food safety is an important issue for the development of the national economy and society. Studying regional food supply and demand from the perspective of land resource carrying capacity can provide new references for regional resource sustainability. This study uses the data from farmer and herdsmen household questionnaires, statistical data, land use data, and other sources to construct a land resource carrying capacity (LCC) assessment framework, targeting the food supply and demand of residents in representative areas, specifically the typical grassland pastoral areas, sandy pastoral areas and agro-pastoral areas on the Xilin Gol grassland transects. The three food nutritional indicators of calories, protein and fat were selected for analyzing the balance of land resource carrying capacity. We found that: 1) Along the Xilin Gol grassland, the main local food supply showed a shift from meat and milk to grains, vegetables and fruits. 2) From north to south along the grassland transects, the calorie intake increased gradually, while the intake of protein and fat was highest in pastoral areas and lowest in agricultural areas. 3) The overall land resource carrying capacity of the Xilin Gol grassland transects was in a surplus state, but the land carrying capacity of typical grassland pastoral area was higher than the two other types of areas. This study provides an empirical reference for the sustainable development of regional food nutrition.  相似文献   

Analog simulation of urban construction land supply and demand in Chang-Zhu-Tan Urban Agglomeration based on land intensive use     

Xiong  Ying  Chen  Yun  Peng  Fen  Li  Jingzhi  Yan  Xiaojing 《地理学报(英文版)》2019,29(8):1346-1362

Journal of Geographical Sciences - Urban land intensive use is an important indicator in harmonizing the relationship between land supply and demand. The system dynamics (SD) can be used to...  相似文献   

Soil Respiration Dynamics and Influencing Factors in Typical Steppe of Inner Mongolia under Long-term Nitrogen Addition     

DU Wei  WU Shanmei  NIE Cheng  LI Yue  SHAO Rui  LIU Yinghui  SUN Nan 《资源与生态学报(英文版)》2019,10(2):155-162

We investigated soil respiration (Rs) dynamics and influencing factors under different nitrogen (N) addition levels (0, 2, 4, 8, 16, 32 g m^-2 yr^-1) on typical grassland plots in Inner Mongolia. We measured soil respiration, temperature, moisture and nutrients. We found that N addition did not change dynamic characteristics of Rs; daily and seasonal dynamics followed a single peak curve. N addition reduced Rs during the growing season. Rs under N2, N4, N8, N16 and N32 treatments decreased by 24.00%, 21.93%, 23.49%, 30.78% and 28.20% in the growing season, respectively, compared to the N0 treatment. However, Rs in the non-growing season was not different across treatments. Rs was significantly positively correlated with soil temperature and moisture and these two factors accounted for 72%-97% and 74%-82% of variation in Rs, respectively. The soil respiration temperature sensitivity (Q₁₀) was between 2.27 and 4.16 and N addition reduced Q₁₀ except in the N8 treatment.  相似文献   

考虑填充墙影响的钢筋混凝土厂房结构地震易损性分析     

梁志强  徐超  温增平 《震灾防御技术》2019,14(4):707-718

采用对角斜撑模拟纵向填充墙的作用，建立考虑填充墙和不考虑填充墙厂房结构模型，采用拉丁超立方抽样技术建立考虑材料不确定性的结构分析样本，基于随机Pushover分析确定结构不同破坏状态下的统计参数。综合考虑结构材料强度及输入地震动不确定性的影响，通过非线性时程分析开展单层钢筋混凝土厂房结构易损性研究，在此基础上比较结构横、纵向易损性的差异，研究填充墙对结构易损性的影响。研究结果表明:钢筋混凝土厂房结构体系横向地震易损性显著大于纵向地震易损性；对纵向结构体系而言，加入填充墙会明显降低结构易损性，但在相同强度的地震动作用下，填充墙破坏程度比主体结构严重，这与厂房结构的实际震害特征相符。  相似文献   

威海市褚岛北部海域CDOM和COD分布特征遥感分析     

黄妙芬  王忠林  邢旭峰  陈亮  孙忠泳 《海洋技术学报》2018,(2):63-70

根据2017年7月和9月在山东威海褚岛北部海域现场测量的COD_(Mn)(Chemical oxygen demand)值和水体表观光学量,结合COMS(Communication,OceanMeteorological Satellite)上搭载的传感器GOCI(Geostationary Ocean Color Imager)所提供的有色可溶性有机物(Chromophoric dissolved organic matter,CDOM)产品,利用星地同步观测数据对现有的基于遥感反射比反演CDOM的模式进行验证,确定适合该海域的CDOM浓度遥感反演模式;通过对测试海域化学需氧量与遥感反演的水体CDOM浓度相关性分析,建立利用CDOM反演COD_(Mn)的遥感模式,并将该模式应用于测试海域LANDSAT 8/OLI(Operational Land Imager)遥感图像上,获取该海域COD_(Mn)浓度专题图,基于这些专题图分析了测试海域COD_(Mn)时空分布特征。结果表明:(1)基于GOCI产品的CDOM浓度值随时间和站点动态变化大,离岸越近数值越高,同一地点水体前后相差近1 h的数值变化也较大;(2)基于LANDSAT 8/OLI遥感数据反演的COD_(Mn)浓度时间动态变化大,总体来看褚岛附近水体的COD_(Mn)含量相对较低,褚岛以北海域水体COD_(Mn)含量有所增加,褚岛西侧水体的COD_(Mn)含量较东侧水体COD_(Mn)含量来说整体偏高。  相似文献