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QIAO Ying WANG Jun MAO Yong LIU Min CHEN Ruanni SU Yongquan KE Qiaozhen HAN Kunhuang ZHENG Weiqiang 《海洋学报(英文版)》2017,36(10):115-119
哈维氏弧菌被观察到与海洋寄生性纤毛虫刺激隐核虫具有内共生现象,其作为一种致病性细菌,可导致感染刺激隐核虫的大黄鱼产生严重的继发性细菌感染。我们通过16s宏测序技术对刺激隐核虫的细菌群落进行鉴定,并通过标准的细菌培养方法分离鉴定出哈维氏弧菌。通过透射电镜和荧光原位杂交方法,在刺激隐核虫的细胞胞质中,均观察到内共生的哈维氏弧菌的存在;而在其细胞核中,则没有相关信号存在。哈维氏弧菌与刺激隐核虫的相关关系以及内共生的哈维氏弧菌对刺激隐核虫的影响作用,仍需要深入进行研究。 相似文献
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Although microbes have traditionally been used as models for testing ecological theory, research on the organization of these communities has largely been isolated from other areas of community ecology. Early studies indicated that microbial populations can form dynamic, interacting assemblages. Observations of distributional patterns suggest that certain deterministic forces regulate community structure. It appears that protozoans are tolerant of a wide range of environmental conditions although evidence for this is largely based on gross environmental analyses. Laboratory studies have suggested the importance of negative and positive biotic interactions in determining community membership, but little field evidence for this exists. The importance of transport processes in controlling community composition is being increasingly recognized. All three types of processes likely act to regulate the colonization and successional dynamics of these communities. A simple model is presented to promote the devel 相似文献
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A variable lacustrine environment in Lake Winnipeg, Manitoba: Evidence from modern thecamoebian distribution 总被引：1，自引：0，他引：1
Kaori Torigai Claudia J. Schröder-Adams Susan M. Burbidge 《Journal of Paleolimnology》2000,23(3):305-318
Thecamoebians (testate protozoan) were examined in 18 surface sediment samples from the North and South basins and the Narrows of Lake Winnipeg, Manitoba. Significantly higher numbers of thecamoebians and tintinnids in the North Basin compared to the Narrows and South Basin are attributed to the effects of urban development around the South Basin of Lake Winnipeg. Human population growth in this area has led to increased nutrient concentration in runoff, causing eutrophication of the southern lake, which in turn allows for increased algal productivity. Cucurbitella tricuspis is found in large abundances in the South Basin, particularly close to the inlet of the Red and Assiniboine rivers. High abundances of this species have been attributed to eutrophic conditions, which this species appears to withstand more successfully than other species. Increases in domestic waste output, that have led to elevated heavy metal concentrations in lake bottom sediments of the South Basin, may have resulted in lower abundances of thecamoebians, further reducing competition.Strong currents in the Narrows cause a slightly coarser substrate and sweep away food sources such as phytoplankton. This results in a lower faunal abundance and slightly lower species richness of thecamoebians. Robust species such as the coarse-grained Difflugia viscidula and species which feed on bacteria such as Centropyxis aculeata show increased abundances.Modern thecamoebian assemblages are comparable to Late Holocene faunal associations in terms of species composition. Individual species abundances, however, have changed. For example, in the North Basin the Late Holocene dominance of Difflugia manicata is replaced by various strains of Difflugia oblonga during recent times. A common species of the South Basin from Late Holocene to recent times is Difflugia globulus. It would appear that faunal differences between basins are the result of differences in algal food sources. 相似文献
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琵琶潭瀑布岩溶沉积物生物多样性研究 总被引：4，自引：1，他引：3
为了探索瀑布岩溶沉积物中生物多样特征, 2006年12月对贵阳市琵琶潭瀑布岩溶沉积环境进行了野外生态学调查,采集样品和标本25份。经室内初步分析和鉴定, 该瀑布岩溶沉积物中含有5大生物多样性类群共33个物种,它们是蓝细菌( Cya no bacteria) 2个种,原生动物( Pr oto zoan) 12个种,绿藻( Green algae ) 1个种,硅藻( Dia toms) 10个种和苔藓植物( Bry ophy tes) 8个种。生物多样性在琵琶潭岩溶沉积形成中的地质生态学贡献主要表现在: ( 1)生物表面为淡水碳酸钙晶体提供了附着基础; ( 2) 绿色植物光合作用转移了二氧化碳,有助于碳酸钙沉积;( 3)生物群落为瀑布岩溶沉积提供了骨架; ( 4) 生物群落在一定程度上控制和影响了岩溶沉积形态。 相似文献
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利用流式细胞仪计数微型浮游生物的方法 总被引：2，自引：0，他引：2
微型浮游生物(细胞粒径20μm)在水生生态系统的物质循环和能量流动中起着重要的作用,对其丰度的准确测定是进一步研究微型浮游生物在不同水生生态系统中作用的重要基础.相对于传统的显微镜检测技术,流式细胞术不仅具有分析速度快、灵敏度和准确度高等优点,而且可以同时测量单个细胞的多个生理参数.不同类型微型浮游生物流式细胞术的应用原理是不同的.对于自养型浮游藻类,主要根据藻体内色素的自发荧光对其进行分辨和计数;而对于异养型细菌、原生动物及浮游病毒等,还需借助外源荧光染料对细胞核酸染色后再进行分析.目前流式细胞术已成为浮游藻类和异养细菌丰度检测的常规方法,但是,由于原生动物具有更大的细胞体积且在自然水体中丰度较低;而浮游病毒粒径又太小,甚至低于光源激发波长,因此流式细胞术应用一直受到限制,直到近10年来才有相关报道.本文对运用流式细胞术计数浮游藻类、浮游细菌、原生动物和浮游病毒的具体原理、方法和进展进行综述,并对流式细胞仪在未来水生微生物学领域的应用进行展望. 相似文献
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贵州香纸沟岩溶藓类附生水生原生动物群落生态特征 总被引：1，自引：0，他引：1
岩溶藓类是一种独特的生态系统类型,维持了丰富的附生水生原生动物群落多样性。2006年11月26日至2007年1月26日, 对贵阳市香纸沟岩溶沉积环境中8种藓类附生水生原生动物群落进行了野外生态调查,采集样品和标本48份。经室内对附生水生原生动物培养、分离和鉴定, 揭示了该区岩溶藓类附生水生原生动物群落具有下述特征: ( 1)水生原生动物群落物种组成含24科26属36种。( 2)水生原生动物群落含6个功能营养群。其中,原生动物光合营养群( P群, Photosy nthetic auto tro phs) , 占总数的25% ; 细菌取食者群( B群, Bactivores detritivores) , 占总数的44. 44% ; 腐生营养类群( S群, Saprotrophs) ,占总数的2. 78% ;食藻者类群( A群, Algivores) ,占总数的11. 11% ; 非选择性杂食者群( N 群,Nonselective omnivores) , 占总数的11. 11% ;食肉者群( R群, Raptor s) ,占总数的5. 56%。( 3)根据岩溶水流、基质和藓类植物特征,划分该区附生水生原生动物生态群落为4个类型和8个亚类型。 相似文献
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微型浮游动物在抑食金球藻(Aureococcus anophagefferens)引发褐潮时表现的摄食压力可潜在控制褐潮的爆发和消亡。本研究就三种海洋常见原生动物——海洋尖尾藻(Oxyrrhis marina)、海洋尾丝虫(Uronema marinum)和扇形游仆虫(Euplotes vannus)——对单种饵料及混合饵料中抑食金球藻中国株的摄食进行了研究。单种抑食金球藻指数期细胞喂食的三种原生动物的生长率和摄食率呈现米氏方程变化趋势。比较三种原生动物摄食抑食金球藻的最大摄食率, 发现其随动物粒径的增大而增大, 但仅为摄食球等鞭金藻(Isochrysis galbana)的30%~59%。海洋尖尾藻和海洋尾丝虫的最大生长率(μmax)与饵料种类无关, 扇形游仆虫摄食抑食金球藻时的μmax值小于摄食球等鞭金藻的个体。海洋尖尾藻、海洋尾丝虫和扇形游仆虫摄食抑食金球藻时的毛生长率(gross growth efficiency, GGE)分别为65.8%、35.2%和49.1%。三种原生动物摄食抑食金球藻指数期细胞和球等鞭金藻以不同比例混合的饵料时表现出对抑食金球藻的选择倾向; 在含有抑食金球藻稳定期细胞的混合饵料喂食的情况下, 三种原生动物避食抑食金球藻或不表现明显摄食倾向性。抑食金球藻释放胞外聚合物(extracellular polymeric substance, EPS)的测定结果显示, 细胞从指数期生长至稳定期释放出的EPS的水平显著上升(P<0.05), 可能与原生动物对不同生长期藻细胞具有不同选择偏好有关。 相似文献
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