Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析          下载免费PDF全文

武翠玲  宋英达  高慧芳  张廉芸  任晨霞 《盐湖研究》2020,28(1)

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性，属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高，α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高，且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸，并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示，α螺旋和β片层围成了蛋白酶的催化腔，EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体，且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性，并有助于其催化功能的发挥，为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。  相似文献   

Correspondence between the large volcanic eruptions and ENSO events over AD 1525–2000     

Fang  Xiuqi  Zheng  Xue  Zhang  Xing 《地理学报(英文版)》2020,30(1):103-118

ENSO is an interannual mode which may be affected by external forcing, such as volcanic eruptions. Based on the reconstructed volcanic eruptions chronology and ENSO sequences, both 195 large volcanic eruptions(VEI≥4) and 398 ENSO(El Ni?o and La Ni?a) events were extracted from 1525 to 2000. An analysis of the correspondence between the large volcanic eruptions and ENSO events was performed by matching the large volcanic eruptions with the types and magnitudes of ENSO events present in the 0–2 years after the eruptions. The results show the following:(1) The percentages of ENSO events within the 3 years after the large eruptions had increased to 68.3% from 31.7% compared with those with no-eruptions in the previous 0–2 years. In addition, the ratio of El Ni?o to La Ni?a events turned from 2:3 to 1:1, and more El Ni?o events occurred in the 0 year after eruptions in the low-latitudes of the Northern Hemisphere and in the tropics but more La Ni?a events occurred in the 0 year after in the high-latitudes of the Northern Hemisphere and the Southern Hemisphere.(2) After the eruptions, the weak(W) El Ni?o events had increased by 8 percentage points and the very strong(VS) El Ni?o events had decreased by 10 percentage points; conversely, there was a decrease by 15 percentage points of the weak La Ni?a events and an increase by 11.4 percentage points of the very strong La Ni?a events. Specifically, the percentages of strong La Ni?a events increased to a peak at 1(+1) year after the eruptions.(3) The percentage of eruptions followed by single-year ENSO was the greatest. The percentage of ENSO events that occurred in the consecutive 2 years following an eruption was approximately equal to the percentage of events that occurred consecutively 3 years following an eruption, and both sets of ENSO magnitudes showed a decreasing trend.  相似文献   

敞开酸溶和偏硼酸锂碱熔ICP-MS法测定多金属矿中的稀土元素及铌钽锆铪   总被引：2，自引：2，他引：0  

门倩妮  沈平  甘黎明  冯博鑫 《岩矿测试》2020,39(1):59-67

用电感耦合等离子体质谱法(ICP-MS)测定地质样品中的稀土及难熔元素,混合酸敞开酸溶法和碱熔融法是两种主要的溶样方法。但地质样品组分复杂,元素之间存在相互共生的现象,对于特殊元素、特殊样品用传统酸溶法会造成部分元素消解不完全,使测定结果不准确;而碱熔法的操作过程繁琐,且溶液盐度高,易产生基体干扰和堵塞仪器进样系统。本文改进了传统四酸和五酸体系,采用氢氟酸-硝酸-硫酸敞开酸溶体系,用国家一级标准物质制作标准曲线测定15种稀土元素,方法准确度(ΔlgC)为0.001～0.027。同时改进了偏硼酸锂碱熔法,样品用偏硼酸锂碱熔提取,加入氢氧化钠调节溶液至碱性条件,所测元素与偏硼酸锂共沉淀后过滤分离熔剂,再用硝酸复溶测定15种稀土元素及铌钽锆铪。两种溶样方法的测定值与认定值的相对误差为1.09%～9.30%。将混合酸敞开酸溶法测定稀土元素、偏硼酸锂碱熔法测定铌钽锆铪的结果与其他实验室密闭酸溶法相比,两组数据的相对偏差为0.13%～15.32%。本实验表明,混合酸敞开酸溶法适用于测定地质样品中的稀土元素,偏硼酸锂碱熔法不仅适用于测定地质样品中的稀土元素及铌钽锆铪,也适用于测定如古老高压变质岩石及铝含量高的样品中的铌钽锆铪。  相似文献   

The δ60/58Ni Values of Twenty‐Six Selected Geological Reference Materials     

Weihan Li  Jian‐Ming Zhu  Decan Tan  Guilin Han  Zhouqiao Zhao  Guangliang Wu 《Geostandards and Geoanalytical Research》2020,44(3):523-535

The high‐precision δ^60/58Ni values of twenty‐six geological reference materials, including igneous rocks, sedimentary rocks, stream sediments, soils and plants are reported. The δ^60/58Ni values of all samples were determined by double‐spike MC‐ICP‐MS (Nu Plasma III). Isotope standard solution (NIST SRM 986) and geological reference materials (BHVO‐2, BCR‐2, JP‐1, PCC‐1, etc.) were used to evaluate the measurement bias and intermediate precision over a period of six months. Our results show that the intermediate precision of Ni isotope determination was 0.05‰ (2s, n = 69) for spiked NIST SRM 986 and typically 0.06‰ for actual samples, and the δ^60/58Ni _{NIST SRM 986} values were in excellent agreement with previous studies. Eighteen high‐precision Ni isotope ratios of geological reference materials are first reported here, and their δ^60/58Ni values varied from ?0.27‰ to 0.52‰, with a mean of 0.13 ± 0.34‰ (2s, n = 18). Additionally, SGR‐1b (0.56 ± 0.04‰, 2s), GSS‐1 (?0.27 ± 0.06‰, 2s), GSS‐7 (?0.11 ± 0.01‰, 2s), GSD‐10 (0.46 ± 0.06‰, 2s) and GSB‐12 (0.52 ± 0.06‰, 2s) could potentially serve as candidate reference materials for Ni isotope fractionation and comparison of Ni isotopic compositions among different laboratories.  相似文献   

DO4事件的精确定年及亚旋回特征: 来自福建龙岩仙云洞石笋的证据^*          下载免费PDF全文

车印平  肖海燕  姜修洋  蔡炳贵 《古地理学报》2020,22(2):377-384

DO4事件(Dansgaard-Oeschger event 4,简称DO4事件)是发生在MIS3阶段后期的1次明显升温事件。根据福建仙云洞石笋(编号XYⅢ-28)300~500 mm层段6个高精度²³⁰Th年龄和188个氧同位素数据,获得了29.13~27.94 kyrB.P.时期平均分辨率达7 yr的东亚夏季风强度的演变过程数据。该石笋 δ¹⁸O 记录的DO4事件起止时间为28.83±0.06~28.46±0.06 kyr B.P.,持续时间为0.37±0.06 kyr。相对基于GICC05年层计数时标NGRIP冰芯 δ¹⁸O 记录在DO4开始阶段的突变特征,仙云洞石笋记录的DO4事件开始阶段 δ¹⁸O 值在145±60 yr内偏负0.4‰,表现为相对缓慢的变化特征,这说明仙云洞所处的中国东南沿海地区有可能受到热带低纬海气耦合的影响。此外,仙云洞石笋 δ¹⁸O 记录揭示了在DO4事件内部及附近存在一系列的百年至十年际尺度夏季风增强事件,与格陵兰冰芯 δ¹⁸O 记录、阿拉伯海海盆反照率记录的DO4事件亚旋回结构在定年误差范围内相对应,表明东亚夏季风强度与北高纬温度变化和ITCZ(赤道辐合带)位置移动在百年至十年际尺度上存在紧密的联系。  相似文献   

基于ROMS和4DVAR的沿轨与网格化SSH数据同化效果评价   总被引：1，自引：1，他引：0  

周超杰  丁效华  张杰  杨俊刚  马强 《海洋学报(英文版)》2018,37(9):50-58

Remote sensing products are significant in the data assimilation of an ocean model. Considering the resolution and space coverage of different remote sensing data, two types of sea surface height(SSH) product are employed in the assimilation, including the gridded products from AVISO and the original along-track observations used in the generation. To explore their impact on the assimilation results, an experiment focus on the South China Sea(SCS) is conducted based on the Regional Ocean Modeling System(ROMS) and the four-dimensional variational data assimilation(4 DVAR) technology. The comparison with EN4 data set and Argo profile indicates that, the along-track SSH assimilation result presents to be more accurate than the gridded SSH assimilation, because some noises may have been introduced in the merging process. Moreover, the mesoscale eddy detection capability of the assimilation results is analyzed by a vector geometry–based algorithm. It is verified that, the assimilation of the gridded SSH shows superiority in describing the eddy's characteristics, since the complete structure of the ocean surface has been reconstructed by the original data merging.  相似文献   

青藏高原东北缘瑞利面波成像：射线理论与程函方程结果的比较     

郝识杰  黄周传  王良书  徐鸣洁  米宁  于大勇 《高校地质学报》2020,26(6):712-720

青藏高原东北缘是青藏高原隆升的前缘地带，其构造变形特征一直是研究的热点。前人在该区域进行过面波成像研究，但不同结果存在较明显的差异，可能与不同成像方法的分辨率有关，此次研究分别使用基于射线的方法和基于程函方程的方法进行了瑞利波相速度成像。笔者对比了20 s、30 s和60 s的成像结果，两种方法的结果表现出相似的速度特征。总体的速度特征与研究区域的主要构造单元分布相吻合，青藏高原东北缘表现出低速异常，鄂尔多斯块体表现出高速异常。在银川河套地堑，基于程函方程的成像在20 s和30 s得到更明显的低速异常，低速异常体的分布与地堑的轮廓吻合更好，说明基于程函方程的方法对数据利用更充分。  相似文献   

差分层析SAR技术研究现状分析     

张月  庞蕾  徐西桂  张天  张瑞菊 《测绘通报》2019,(1):5-12

差分层析SAR是在层析SAR基础上发展起来的一种四维信息反演技术。它不仅实现了对雷达目标的三维分辨能力，同时可以获取目标的形变速率信息，可实现对目标方位-距离-高度-时间四维成像，这对实现城市基础设施动态形变监测、古建筑风险评估、重要工程安全监测等应用具有重要实际意义和价值。本文基于差分层析SAR成像原理，分析了成像处理过程中存在的问题，总结了差分层析SAR成像算法研究现状和特点；最后列举了差分层析SAR技术的主要应用领域，并对其技术发展趋势进行了展望。  相似文献   

Late Holocene changes in vegetation and atmospheric circulation at Lake Uddelermeer (The Netherlands) reconstructed using lipid biomarkers and compound‐specific δD analysis          下载免费PDF全文

《第四纪科学杂志》2018,33(1):100-111

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代表性人口空间分布数据集的精度评价——以2010年广东省为例   总被引：1，自引：0，他引：1  

林丹淳  谭敏  刘凯  柳林  朱远辉 《热带地理》2020,40(2):346-356

以人口密度差异显著的广东省为研究区,比较Worldpop、GPW v4和2种中国公里网格人口分布数据集的空间分布一致性,并以第六次全国人口普查数据为真值,按人口密度分为高、中、低3组,从误差的数值分布和空间分布两方面定量评价4种数据集的精度,最后讨论估算误差的可能来源及数据适用性。结果表明,4种网格人口数据集中Worldpop整体精度最高,且在人口密集区的精度也是最高;GPW v4在低人口密度和中人口密度区域精度略高于Worldpop,但对镇街内人口分布细节刻画不够详细;2种中国公里网格人口分布数据集精度较前两者低,主要受空间化方法和模型变量的选择所限制。Worldpop适合用于人口密度中等及人口密度高区域的精细化研究,GPW v4适合用于长时序、最小研究单元大于镇街的研究,第一种中国公里网格人口分布数据集适合用于需要考虑镇街内人口分布和空间异质性的研究,第二种中国公里网格人口分布数据集适用于需要考虑人口分布细节和空间格局变化的长时序研究。  相似文献