倒置显微镜拍照法对浮游植物计数的稳定性分析     

陈明华  史康佳  李志杰  蔡菲  张浩  张建辉  胡文涛 《湖泊科学》2020,32(5):1564-1574

准确定量分析湖泊河流中浮游植物群落结构对评价湖泊河流健康和保障人类饮水安全至关重要.显微镜镜检法是定量分析浮游植物群落结构的唯一方法,但不能满足浮游植物计数分析要求.在Utermhl倒置计数管视野法基础上提出倒置显微镜拍照法,使用拍照法对小球藻、四尾栅藻、斜生栅藻3种纯藻样品和鄱阳湖野外样品计数得到:1)在5、10倍物镜下拍照因浮游植物个体太小导致计数不准确,在20、40倍物镜下拍照计数准确,增加拍照次数可较好地避免计数偏差,拍照次数是影响拍照法计数分析的主要因素; 2)小球藻、四尾栅藻、斜生栅藻在20和40倍物镜下分别拍摄5和10张照片时每升浮游植物计数稳定;鄱阳湖野外样品在20和40物镜倍下分别拍摄5、10张照片时每升浮游植物计数稳定; 3)倒置显微镜拍照法比计数框行格法、目镜视野法、目镜行格法和Utermhl倒置计数管视野法计数稳定,变异系数最小为0.812.  相似文献   

Timings of early crustal activity in southern highlands of Mars:Periods of crustal stretching and shortening     

Trishit Ruj  Goro Komatsu  Jan Hendrik Pasckert  James M.Dohm 《地学前缘(英文版)》2019,10(3):1029-1037

Extensional and compressional structures are globally abundant on Mars. Distribution of these structures and their ages constrain the crustal stress state and tectonic evolution of the planet. Here in this paper, we report on our investigation over the distribution of the tectonic structures and timings of the associated stress fields from the Noachis-Sabaea region. Thereafter, we hypothesize possible origins in relation to the internal/external processes through detailed morphostructural mapping. In doing so, we have extracted the absolute model ages of these linear tectonic structures using crater size-frequency distribution measurements, buffered crater counting in particular. The estimated ages indicate that the tectonic structures are younger than the mega impacts events(especially Hellas) and instead they reveal two dominant phases of interior dynamics prevailing on the southern highlands, firstly the extensional phase terminating around3.8 Ga forming grabens and then compressional phase around 3.5-3.6 Ga producing wrinkle ridges and lobate scarps. These derived absolute model ages of the grabens exhibit the age ca. 100 Ma younger than the previously documented end of the global extensional phase. The following compressional activity corresponds to the peak of global contraction period in Early Hesperian. Therefore, we conclude that the planet wide heat loss mechanism, involving crustal stretching coupled with gravitationally driven relaxation(i.e.,lithospheric mobility) resulted in the extensional structures around Late Noachian(around 3.8 Ga). Lately cooling related global contraction generated compressional stress ensuing shortening of the upper crust of the southern highlands at the Early Hesperian period(around 3.5-3.6 Ga).  相似文献   

野外现场铀矿石中铀的快速准确测定方法研究——TOPO萃取α计数法     

黄秋红  刘立坤  郭冬发  王玉学  武朝晖  汤三星  李红光 《岩矿测试》2011,30(4):423-429

研究了野外现场快速准确测定铀矿石中铀的三辛基氧膦(TOPO)萃取α计数法。目前野外现场铀的测量主要采用γ谱法,即利用铀子体的γ射线强度来计算铀的含量,当样品中铀镭处于不平衡状态时,γ谱法测铀存在着较大的测量误差。本文将铀的核性质与化学性质结合起来,采用密闭酸溶法快速溶解样品中的铀,酸溶后的样品不经分离直接在溶样罐中用TOPO萃取,进而测定样品中铀产生的α射线强度获得铀的含量,避免了γ谱法的不足。方法检出限为铀含量2.41μg/g;当铀含量为大约为100μg/g时,测量相对偏差为5.93%;铀的测定范围为7.23μg/g～n%。密闭酸溶法试剂用量少,溶样速度快,且对环境和操作人员污染小;酸溶后的样品用TOPO萃取2 min后即可达到萃取平衡,铀萃取效率在97%以上;所需的仪器设备可以车载形式用于野外现场铀矿石中铀的准确测定,野外应用操作简单、快速、精密度和准确性较高。  相似文献   

基于光子计数探测器的能谱CT的研究     

徐品  陈奭  袁刚  张寅  郑健  邢晓曼  孙明山 《CT理论与应用研究》2015,(6):819-825

本文的目的是评估光子计数探测器对CT成像的性能的影响,我们对一款基于碲锌镉(CZT)的光子计数探测器进行了性能测试,并搭建一个成像平台。首先对探测器系统进行能量增益的校正以及坏像素点校正,以减少探测器响应的不均匀性对CT成像的影响。然后对探测器进行能量-阈值标定,以确定探测器的工作能量阈值。最终搭建成像系统,对X光能量分别大于36和45ke V的数据,获得了模体的重建结果,并对结果进行了分析。  相似文献   

一种改进的计数型TDC设计及实现     

王磊  郭唐永  庞聪 《大地测量与地球动力学》2017,37(9):987-990

基于常规计数型TDC设计思想，通过FPGA芯片的内部PLL延迟相位技术和内部资源构建延迟链改进常规计数型TDC，时间间隔测量精度可达200 ps。该方法对激光测距、三维激光扫描仪、绝对重力仪等仪器中精密时间测量系统的研究具有实际意义。  相似文献   

厌氧沉积中有孔虫组合特征     

程广芬 《中国海洋大学学报(自然科学版)》1988,(1)

厌氧沉积的有孔虫组合中,底栖有孔虫主要由Bolivina诸种和Buliminella tenuata组成,同时含有少量厌氧标志种Suggrunda eckisi;浮游有孔虫中以Globigerrina bulloides和G.quingueloba为数较多。底栖有孔虫与浮游有孔虫数量相比,浮游有孔虫数量很少。  相似文献   

冬季和春季长江口及其近海水域浮游病毒丰度的分析   总被引：7，自引：2，他引：7       下载免费PDF全文

白晓歌  汪岷  马晶晶  孙军  梁彦韬  乔倩 《海洋与湖沼》2007,38(4):367-372

采用荧光显微技术,对2006年长江口及近海水域20个站点的表层及10m层或潜水体冬、春两季的浮游病毒丰度进行了检测,对浮游病毒丰度在季节（冬、春两季）、水平分布和垂直分布上的变化进行了探讨.调查区浮游病毒丰度在冬、春季节上并无明显差异,但在水平分布上存在很大差异,河口区浮游病毒直接检测量（Virus Direct Count, VDC）达到10^7个/ml,近海水域VDC为10^6个/ml,河口区的浮游病毒丰度都明显高于近海水域病毒丰度 （P＜0.01）.在垂直分布上,冬、春两季长江口水域水深小于10m的站位,表层浮游病毒丰度与底层病毒丰度无明显差别,水深大于10m的站位,表层水样的浮游病毒丰度都高于10m水层病毒丰度,说明长江口浮游病毒的垂直分布与站位总水深有关.还通过比较各站点VDC与叶绿素a含量的数据,分析了二者之间的相关性：冬季浮游病毒丰度与叶绿素a含量成正相关性;春季浮游病毒丰度与叶绿素a含量成负相关性,但病毒丰度受叶绿素a含量的影响仅为10%-11%.  相似文献   

Rapid assessment of beta dose variation inside cobbles,and implications for rock luminescence dating     

《Quaternary Geochronology》2022

Variation in beta dose rate within rocks may impact the results of rock surface luminescence dating, for both the burial age of cobbles and exposure age of rock surfaces. Current methods of rock surface luminescence dating assume that radionuclides are homogeneously distributed inside rocks. In this study, two rapid methods based on beta counting and on a portable XRF instrument were developed to measure the radioactivity of rock slices. These methods were applied to rock slices from four glaciofluvial granite cobbles that had previously been used for equivalent dose determination to test whether beta dose variation could be observed. Results from beta counting and K content from XRF show similar patterns and both vary along the depth profiles, but the magnitude of this variability is very different amongst the four cobbles. In rocks where the dose rate is highly variable, bleaching may not be the only source of variation of L_n/T_n or equivalent dose (D_e) along the luminescence-depth profile of cobbles, and it may be necessary to measure the beta dose rate for every single slice to determine whether multiple bleaching events are recorded or variations in D_e are due to dose rate heterogeneity.  相似文献   

Reinvestigation of the irregularities in the 3H decay     

《Astroparticle Physics》2013

Having in mind the potential impact of the results presented by Veprev and Muromtsev (2012) [13] on our knowledge of the universe, we reinvestigated the liquid scintillation measurement of the count rate variations of ³H. Making use of the sophisticated Quantulus liquid scintillation spectrometer, we found that the measurement of the high-energy tail of ³H spectrum may be significantly influenced by instrumental instability. Thus, the possible explanation for the relatively high count rate variations of Veprev and Muromtsev (2012) [13] can be attributed mainly to the walk of the cut-off in the integrated spectrum, although weak variations of different origin could be masked by such cut-off drifts. In our experiment we have also registered the oscillatory behavior of measured high-energy tail of ³H spectrum, but with very small amplitude (less than 0.5%), which cannot be easily explained only by instrumental instability. When the total ³H spectrum was measured, no significant variations in the count rate were found.  相似文献   

湖泊沉积物年纹层的研究方法及其意义   总被引：12，自引：4，他引：8  

刘强  游海涛  刘嘉麒 《第四纪研究》2004,24(6):683-694

文章总结了前人对湖泊沉积物年纹层类型的划分,并根据年纹层的形成过程和组分特征分为3个大类,即碎屑年纹层、生物成因年纹层(如硅藻年纹层等)和化学成因年纹层(如方解石年纹层、菱铁矿年纹层、黄铁矿年纹层、蒸发盐年纹层等)。介绍了目前应用于湖泊沉积物年纹层研究的主要方法和技术包括:1)新鲜沉积物表面照相、X射线照相技术;2)光学显微镜观察;3)数字化图像分析;4)扫描电子显微镜技术等。而岩相学大薄片是目前年纹层研究工作中应用非常广泛的材料,对目前制作大薄片比较普遍采用的快速冷冻-冷冻干燥和水-丙酮-环氧树脂交换这两种方法进行了介绍并比较了各自的优劣。湖泊沉积物年纹层研究的古环境意义主要体现在两个方面,一是提供了高精度的纹层年代学时间标尺,在诸如气候突变事件的时限、大气¹⁴C浓度变化、火山灰层定年、古地磁场长期变化主曲线重建等方面有重要意义;二是年纹层性质研究如年纹层厚度和年纹层微相变化本身所蕴藏的高分辨率古气候环境变化信息,在太阳活动周期、ENSO等气候事件的研究中也有重要意义。最后,文章简单介绍了我国东北龙岗火山区四海龙湾玛珥湖沉积物中年纹层的特征并展望了其研究潜力。  相似文献