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吉林省第五地质调查所在吉林壳牌合资公司舒兰、敦化、松源油页岩项目中,针对具体的地层情况,制定了有效的施工工艺和钻井液配置,取得了较好的效果.详细介绍了舒兰5号井、敦化3号井、松原ZK0833号井的钻探技术措施及其钻井液配置技术. 相似文献
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KANG Kyoung Ho 《中国海洋大学学报(英文版)》2009,8(3):265-269
Trumpet shell, Charonia sauliae, is an endangered and valuable species, but its artificial propagation protocol has not been successfully established. To
estimate the possibility of cryopreservation for larvae of C. sauliae, which is a potential preparation for its artificial reproduction and further research, in this study a protocol for the
cryopreservation of veliger larvae of trumpet shell was optimized. Through a two-step cryopreservation procedure, four kinds
of cryoprotectants (ethylene glycol, 1, 2-propanediol, dimethyl sulfoxide and glycerol) were employed at three concentrations
(1.0, 1.5 and 2.0 molL−1) respectively and survival rates of larvae were determined after a storage of 1h. The larvae frozen with these four cryoprotectants
after 1 h storage were cultured, and then survival rates were determined at 24, 72 and 120 h after thawing. Dimethyl sulfoxide
at a concentration of 1.5 molL−1 showed the best protective effect in all experiments (p<0.05). And survival rates of larvae frozen with dimethyl sulfoxide were determined after 1, 7 and 15 d of storage. The survival
rates of larvae frozen with 1.5 molL−1 dimethyl sulfoxide after 1 h, 1 d, 7 d and 15 d of storage were 80.77% ±7.51%, 80.34% ±11.28%, 83.10% ±9.14% and 77.23% ±6.22%
respectively. No significant differences in survival rates of larvae frozen with dimethyl sulfoxide were observed after various
storage periods (p>0.05). 相似文献
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