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排序方式: 共有13条查询结果,搜索用时 16 毫秒
1.
将不同浓度PM_(2.5)降尘作用于A549细胞后,利用MTT法检测其存活率,W-G染色观察细胞形态,荧光探针法检测细胞ROS和MMP相对水平,以探讨PM_(2.5)降尘对A549细胞线粒体氧化损伤的影响及作用机制。结果显示,经12. 5μg/m L的PM_(2.5)降尘作用A549细胞3 h后,细胞存活率为(81. 77±6. 15)%,并随作用浓度及时间增加呈递减趋势。PM_(2.5)染毒后可观察到细胞形态不规则,胞膜溶解破坏,细胞微核出现。PM_(2.5)降尘作用于细胞24 h后,胞内ROS相对含量随暴露浓度增加呈递增趋势,细胞MMP相对水平随染毒浓度增加而降低,且胞内ROS和MMP两者间存在显著相关关系(R2=0. 878)。提示PM_(2.5)降尘处理A549细胞后可通过刺激ROS的产生,诱导细胞MMP下降,造成细胞线粒体氧化损伤。  相似文献   
2.
ABSTRACT

Rain-on-snow (ROS) has the potential to produce devastating floods by enhancing runoff from snowmelt. Although a common phenomenon across the eastern United States, little research has focused on ROS in this region. This study used a gridded observational snow dataset from 1960–2009 to establish a comprehensive seasonal climatology of ROS for this region. Additionally, different rain and snow thresholds were compared while considering temporal trends in ROS occurrence at four grid cells representing individual locations. Results show most ROS events occur in MAM (March-April-May). ROS events identified with rainfall >1 cm are more frequent near the east coast and events identified with >1 cm snow loss are more common in higher latitudes and/or elevations. Decreasing trends in DJF (December-January-February) ROS events were identified near the coastal areas, with increasing trends in the northern portion of the domain. Significant decreasing trends in MAM ROS are likewise present on a regional scale. Factors playing a role in snowpack depth and rainfall, such as movement of storm tracks in this region, should be considered with future work to discern mechanisms causing the changes in ROS frequency.  相似文献   
3.
UV-B辐射对青岛大扁藻生长及其某些生理特性的影响   总被引:3,自引:0,他引:3  
通过实验生态学和生物化学的方法,研究了UV-B辐射对青岛大扁藻生长及其叶绿素a(Chl-a)含量、可溶性蛋白含量、过氧化氢(H2O2)含量、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力的影响。结果表明:UV-B辐射会抑制青岛大扁藻的生长并对其生理特性产生明显的影响。经过UVB辐射后,Chl-a含量和可溶性蛋白含量在初始阶段(0~3d)有所增加,而后却出现极显著的下降;过氧化氢含量、丙二醛含量和超氧化物歧化酶活力则呈现升高的趋势。  相似文献   
4.
我国新疆北部地区雪面雨日数时空变化特征分析   总被引:1,自引:1,他引:0  
在全球变化背景下,雪面雨发生频次增加,致灾风险加大,认识雪面雨时空变化特征对于防洪减灾具有重要意义。基于我国新疆北部地区42个国家气象站1960—2015年逐日气温、降水、雪深、天气现象等气象观测数据,制定降水类型、地面状态、雪深等共同判定雪面雨事件的参数化方案,进而分析新疆北部地区雪面雨日数时空变化特征及其与气温、海拔的关系。结果表明:近56 a来新疆北部地区雪面雨日数以0.3 d·(10a)-1的速率呈缓慢增加趋势;空间分布上,新疆北部地区雪面雨主要集中于塔城北部、伊犁河谷、乌鲁木齐河源地区,其中塔城裕民县最多,年平均雪面雨日数12.2 d;相关分析显示雪面雨日数及雪面雨量均与海拔呈显著正相关。该研究有望提升对新疆干旱区雪面雨事件这一诱发雨雪混合洪水重要现象的科学认识,为新疆地区致灾洪水过程分析以及洪水监测预警提供参考。  相似文献   
5.
孟浩  陈苏婷 《测绘科学》2015,40(3):85-90,51
针对已有的特征点检测算法的不足,文章提出了一种基于自适应支撑区域(ROS)的算法,该算法针对目前特征点检测中的支撑区域选择,提出了一种自适应单调检测方法,改变了传统支撑区域选择过于单一的难点,自适应性强;并对于曲率的非极大值抑制易造成误检的问题,给出了三次扫描算法,即分析误检产生机理筛选候选特征点。通过支撑区域的自适应选择和三次扫描,实现了算法的自适应性和鲁棒性,检测精度高。试验结果表明,该算法无需先验参数,运行效率高,算法复杂度低。  相似文献   
6.
采用生化测定的方法,对锯缘青蟹(Scyllaserrata)鳃、肝胰腺和肌肉等不同器官组织中活性氧(ROS)与抗超氧阴离子自由基(ASAFR)含量进行了测定。 结果表明, 不同器官组织中活性氧与抗超氧阴离子自由基含量各异。 各器官组织中活性氧含量由高到低依次为:肝胰腺>肌肉>鳃, 分别为195.04U/mg±33.46U/mg, 64.03 U/mg±12.77 U/mg和44.84U/mg±10.44U/mg。而抗超氧阴离子自由基含量则是鳃>肝胰腺>肌肉,大小分别为439.62 U/g±64.73U/g,297.89 U/g±39.75 U/g和84.24 U/g±18.03 U/g。统计分析显示,锯缘青蟹各器官组织间的活性氧与抗超氧阴离子自由基含量均存在极显著性差异(P<0.01)。这是由于不同器官组织所执行的生理功能不同造成的。  相似文献   
7.
皱纹盘鲍血细胞吞噬发光的研究   总被引:6,自引:1,他引:5  
张峰  李光友 《海洋与湖沼》2000,31(4):386-391
于1998年7月从大连碧龙海珍品有限公司购得人工养殖的皱纹盘鲍,运用化学发光法研究在体外条件下利用6种活性氧清除剂SOD(超氧化物歧化酶)、CAT(过氧化氢酶)、苯甲酸钢和DMF(二甲基呋喃)、NBT(硝基四唑蓝)及金属螯合济EDTA(乙二胺四乙酸)等试齐对皱纹盘鲍血细胸吞噬活动中化学发光的产生和影响。结果表明,活性氧清除剂对皱纹盘鲍血细胞的吞噬发光都有掏作用,说明皱纹盘鲍血细胞在吞噬活动中能够释  相似文献   
8.
Oxidative stress by increased production of reactive oxygen species such as superoxide has been implicated in the toxicity of PCB's and non-target toxicity of many pesticides. We report the development of a microplate-based method for determination of early stage oxidative stress using an established cell line (EPC) from a skin tumour of carp Cyprinus carpio L. and 2',7'-dichlorodihydrofluorescein diacetate (H(2)-DCFDA) as a fluorescent probe for detection of reactive oxygen species (ROS) formation. Sublethal concentrations of the herbicide Paraquat, an established redox cycling agent and a crude PCB mixture, Arochlor 1254 elicited a linear increase in ROS formation over 2 h exposure which was some 45- and 10-fold higher, respectively, than attributable to basal respiration, confirming the suitability and response of the test system. Whilst in vivo studies in mammals have implicated early stage oxidative stress in the toxicity of pesticides, we did not observe an increase in ROS production after exposure of EPC cells to sublethal concentrations of Carbaryl, 2,4-DDT, Lindane or Malathion implying that this is not the causative mechanism of acute toxicity in this fish cell line. The apparent involvement of oxidative stress in their mammalian toxicity may therefore be an indirect effect or dependent upon compound metabolism.  相似文献   
9.
10.
Different amounts of vitamin C were added to diets fed to juveniles(2.5 ± 0.15 g) of sea cucumber Apostichopus japonic u s(Selenka) in an attempt to reduce the stress response of specimens exposed to nitrite stress. A commercial feed was used as the control diet and three experimental diets were made by supplementing 1 000, 1 500, or 2 000 mg vitamin C/kg diet to control diet separately in a 45-day experiment. Sea cucumbers were exposed to three different levels(0.5, 1.0, and 1.5 mg/L) of nitrite stress for 4, 8, and 12 h at four time intervals(0, 15, 30, and 45 d). Growth of the animals was recorded during the experiment. Reactive oxygen species(ROS)(i.e. hydroxyl free radical(-OH), malondialdehyde(MDA) and total antioxidant capacity(T-AOC)) and antioxidant enzyme activities(i.e., superoxide dismutase(SOD) and catalase(CAT)) were measured. Response surface methodology(RSM) was used to analyze the effect of multiple factors on ROS indices and enzyme activities. Weight gain(WG) and special growth rate(SGR) of vitamin C supplementation groups were significantly higher than those of control group( P 0.05). The levels of-OH and MDA increased under exposure time extending and nitrite concentration increasing, whereas T-AOC level decreased. SOD and CAT activities increased at 4 h and 8 h and decreased at 12 h. During the days in which the animal consumed experimental diets, the levels of-OH and MDA decreased and that of T-AOC increased. This result suggests that diets containing vitamin C could reduce the nitrite stress response in the animals and increase their antioxidant capacity. The multifactor regression equation of growth performance, ROS indices, and duration of feeding results suggest that vitamin C supplementation of 1 400–2 000 mg/kg diet for 29–35 days could reduce effectively the effects of nitrite exposure.  相似文献   
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