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本研究从条斑紫菜中克隆了一个丝裂原活化蛋白激酶(mitogen activated protein kinase)基因,命名为PyMAPK3。该基因全长2155bp,开放阅读框(ORF)1401bp,编码466个氨基酸,含有两个内含子。氨基酸序列分析表明,PyMAPK3磷酸化位点的氨基酸基序为TDY,其等电点为7.17,蛋白分子量为51.6KDa,亚细胞定位于叶绿体。荧光定量PCR技术对该基因在不同非生物胁迫条件下的转录差异分析结果显示,极端胁迫条件下基因表达量显著增加。蛋白免疫印迹技术对蛋白在不同胁迫条件下的表达量分析结果显示,在不同的胁迫条件下其翻译水平的表达模式表现出与转录水平的不一致性。本研究为深入了解条斑紫菜在逆境适应机制中MAPK蛋白的作用奠定基础,同时为条斑紫菜抗逆品系的选育提供理论指导。 相似文献
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Bangiales is the only order of the Bangiophyceae and has been suggested to be monophyletic. This order contains approximately 190 species and is distributed worldwide. Previous molecular studies have produced robust phylogenies among the red algae, but the divergence times, historical biogeography and evolutionary rates of Bangiales have rarely been studied. Phylogenetic relationships within the Bangiales were examined using the concatenated gene sets from all available organellar genomes. This analysis has revealed the topology((( Bangia, Porphyra) Pyropia) Wildemania). Molecular dating indicates that Bangiales diversifi ed approximately 246.40 million years ago(95% highest posterior density(HPD)= 194.78–318.24 Ma, posterior probability(PP)=0.99) in the Late Permian and Early Triassic, and that the ancestral species most likely originated from eastern Gondwanaland(currently New Zealand and Australia) and subsequently began to spread and evolve worldwide. Based on pairwise comparisons, we found a slower rate of nucleotide substitutions and lower rates of diversifi cation in Bangiales relative to Florideophyceae. Compared with Viridiplantae(green algae and land plants), the evolutionary rates of Bangiales and other Rhodophyte groups were found to be dramatically faster, by more than 3-fold for plastid genome(ptDNA) and 15-fold for mitochondrial genome(mtDNA). In addition, an average 2.5-fold lower dN/dS was found for the algae than for the land plants, which indicates purifying selection of the algae. 相似文献
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石花菜(Gelidiumamansii)是1种重要的大型经济海藻,本研究采用AFLP技术对青岛石老人海域石花菜的遗传结构进行了分析,7对引物组合共扩增出346个位点,多态性比例为47.8%。不同个体间的遗传距离分布范围在0.029~0.390之间。群体内Na(观测等位基因数)为1.504 3,Ne(有效等位基因数)为1.298 5,Nei's基因多样性指数为0.175 5,Shannon信息指数达到0.263 3。UPGMA聚类分析将30个个体分为2个大的类群,在一定程度上反映了石老人海域石花菜群体遗传多样性不高的现状。 相似文献
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