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盐度对细基江蓠繁枝变型的生长、琼胶产量和组成的影响 总被引：2，自引：0，他引：2
在实验室中用盐度为21和33的两种水体培养细基江蓠繁枝变型4个星期，测定了江蓠的生长率，并用水和不同浓度的乙醇溶液分级提取法分析了琼胶的产量和组成情况。结果显示，江蓠在盐度为21的水体中生长较好，其日平均生长率比高盐度（33）组的高约58%；在盐度为33下的琼胶产量较高，平均可达藻体干重的31%，琼胶组成中以冷水提取物和40%的乙醇提取物产量最高，分别占琼胶总产量的20%以上，盐度对琼胶的组成有影响，在21的盐度下，高温高压提取物和60%的乙醇提取物产量高于同等条件下盐度为33的江蓠，而其冷水提取物产量则较低。 相似文献
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条斑紫菜单细胞固体培养的初步研究 总被引：1，自引：0，他引：1
成熟条斑紫菜叶状体酶解后获得单细胞,分别培养于添加了不同浓度N,P营养盐的0．5％和10％的琼胶培养基上,观察细胞的分化发育情况。结果显示：(1)营养细胞大部分先发育为细胞团,细胞团放散孢子,再由孢子萌发形成小苗。其余营养细胞极性分裂形成正常细胞苗或带假根的形状不规则的片状体。孢子茁和营养细胞直接形成的幼苗生长到一定大小均可放散单孢子,单孢予能够正常萌发形成单孢子苗。(2)在0．5％的琼胶培养基上或较高浓度的营养盐中,细胞发育快。添加5mg／L NO3-N和0．5mg／L H2PO4—P营养盐不能满足细胞生长发育的需要,培育20d绝大部分仍为单细胞,不能形成正常幼苗。 相似文献
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To establish a micropropagation system of three Laurencia complex species (Laurencia okamurai, Laurencia tristicha, and Chondrophycus undulatus) by tissue culture techniques, we studied the regeneration characteristics and optimal culture conditions of axenic algal
fragments cultured on solid medium and in liquid medium. Regeneration structures were observed and counted regularly under
a reverse microscope to investigate the regeneration process, polarity and optimal illumination, and temperature and salinity
levels. The results show that in most cultures of the three species, we obtained bud regeneration on solidified medium with
0.5% agar and in liquid medium. Rhizoid-like regeneration was filamentous and developed from the lower cut surface of fragments
in L. okamurai, but was discoid and developed from the apical back side of bud regeneration in L. tristicha and C. undulatus. Regeneration polarity was localized to the apical part of algal fronds in all three species, and on fragments cut from the
basal part of algae buds could develop from both the upper and the lower cut surfaces. Buds could develop from both the medullary
and the cortical portions in L. okamurai and C. undulatus, while in L. tristicha, buds only emerged from the cortex. The optimal culture conditions for L. okamurai were 4 500 lx, 20°C and 35 (salinity); for C. undulatus, 4 500 lx, 20°C and 30; and for L. tristicha, 4 500 lx, 25°C and 30. 相似文献
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To establish a micropropagation system of three Laurencia complex species (Laurencia okamurai, Laurencia tristicha, and Chondrophycus undulatus) by tissue culture techniques, we studied the regeneration characteristics and optimal culture conditions of axenic algal fragments cultured on solid medium and in liquid medium. Regeneration structures were observed and counted regularly under a reverse microscope to investigate the regeneration process, polarity and optimal illumination, and temperature and salinity levels. The results show that in most cultures of the three species, we obtained bud regeneration on solidified medium with 0.5% agar and in liquid medium. Rhizoid-like regeneration was filamentous and developed from the lower cut surface of fragments in L. okamurai, but was discoid and developed from the apical back side of bud regeneration in L. tristicha and C. undulatus. Regeneration polarity was localized to the apical part of algal fronds in all three species, and on fragments cut from the basal part of algae buds could develop from both the upper and the lower cut surfaces. Buds could develop from both the medullary and the cortical portions in L. okamurai and C. undulatus, while in L. tristicha, buds only emerged from the cortex. The optimal culture conditions for L. okamurai were 4 500 lx, 20°C and 35 (salinity); for C. undulatus, 4 500 lx, 20°C and 30; and for L. tristicha, 4 500 lx, 25°C and 30. 相似文献
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ＩＮＴＲＯＤＵＣＴＩＯＮＩｎｒｅｃｅｎｔｙｅａｒｓ,ｃｏ ｃｕｌｔｕｒｅ (ｉｎｔｅｇｒａｔｅｄｃｕｌｔｕｒｅ )ｏｆｍａｒｉｎｅｓｐｅｃｉｅｓ (ａｌｇａｅａｎｄａｎｉｍａｌｓ)ｈａｓｒｅｃｅｉｖｅｄｇｒｅａｔａｔｔｅｎｔｉｏｎ .Ｉｎｃｏ ｃｕｌｔｕｒｅｓｙｓｔｅｍｔｈｅａｌｇａｅａｃｔｉｖｅｌｙｕｐｔａｋｅＣＯ2 ａｎｄｔｈｅｍｅｔａｂｏｌｉｃｗａｓｔｅｓ(ｓｕｃｈａｓＮＨ4 +,ＮＯ2 - ,ＮＯ3- ｅｔｃ)ａｓｎｕｔｒｉｅｎｔｓｏｕｒｃｅｓ,ａｎｄｒｅｌｅａｓｅＯ2 ｔｏｔｈｅｓｕｒｒｏｕｎｄｉｎｇｅｎｖｉｒｏｎ … 相似文献
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Gracilaria tenuistipitata var Liui were mono-cultivated and co-cultivated with Pinctada martensii under high(33) and low(21) salinity conditions in laboratory.The daily growth rate of the alga was determined.Tissue carbon and nitrogen contents,the yield and fractional composition of agar were analyzed.Results showed that:1.Gracilaria grew better under low salinity conditions,the daily growth rate was twice that under high salinity conditions.Co-cultivated algae grew faster than mono-culti-vated algae under low salinity conditions,the daily growth rate was about 37.6?higher.2.Compared with mono-cultivated algae,tissue nitrogen contents of co-cultivated algae were higher,while the C:N ratios were much lower.3.The agar yields of co-cultivated algae were much lower than those of mono-culti-vated algae.Agar yield was found to be negatively correlated to the tissue nitrogen contents,and positively correlated to the C:N ratios.4.The highest fractional yields obtained from co-cultivated algae were extracted with 40?ethanol,while from mono-cultivated algae,the highest fractional yields obtained were extracted with distilled water at room temperature. 相似文献
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Gracilaria tenuistipitata vahang et Xia is cultivated at two different salinites(21,33) in the laboratory for 4 weeks,The daily growth rate is determined.The total agar yield and fractional agar composition are analyzed.Results show that algae grow faster in low salinity.The total agar yield is higher under high salinity conditions than under low salinity conditions.Among the eight fractions extracted,the yields of cold water extract and 40% ethanol extract are about the highest.In low salinities the yields of autoclaved extract and 60% ethanol extract are higher,while the yield of cold water extract is lower relative to high salinities. 相似文献
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