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提取质粒进行PCR和酶切鉴定人粒细胞集落刺激因子(rhG-CSF)基因成功转入鱼腥藻7120中,并用酶联免疫方法测得转基因鱼腥藻7120-G-CSF中G-CSF蛋白在第21d表达量达到最大。对转基因蓝藻的生长曲线、叶绿素a含量测定结果表明,rhG-CSF基因转化蓝藻后,对蓝藻前中期的生长影响不大,但21d后生长速度下降,同时外源蛋白表达也达到最大值,这有利于转基因藻的大规模培养。  相似文献
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Waves generated by vertical seafloor movements are simulated by use of a fully nonlinear two-dimensional numerical wave tank. In the souree region, the seafloor lifts to a designated height by a generation function. The numerical tests show that the linear theory is only valid for estimating the wave behaviors induced by the seafloor movements with a small amplitude, and the fully nonlinear numerical model should be adopted in the simulation of the wave generation by the large amplitude seafloor movements. Without the background surface waves, many numerical tests on the stable maximum elevations η0^max are carried out by beth the linear theory and the fully nonlinear model. The results of two models are compared and analyzed. For the fully nonlinear model, the influences of the amplitudes and the horizontal lengths on η^max are stronger than that of the characteristic duration times. Furthermore, results reveal that there are significant differences be- tween the linear theory and the fully nonlinear model. When the influences of the background surface waves are considered, the corresponding numerical analyses reveal that with the fully nonlinear model the η0^max near-linearly varies with the wave amplitudes of the surface waves, and the η0^max has significant dependences on the wave lengths and the wave phases of the surface waves. In addition, the differences between the linear theory and the fully nonlinear model are still obvious, and these differences are significantly affected by the wave parameters of the background surface waves, such as the wave amplitude, the wave length and the wave phase.  相似文献
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基于埃克曼理论,本文将波致应力和飞沫应力引入到海-气边界层的界面应力中,来研究海表面风浪和海洋飞沫对海-气边界层动量交换的影响,并得到修改后的埃克曼模型的理论解。波致应力是由风浪谱和波增长函数估计,并得到在中低风速下,波致应力、飞沫应力与湍流应力相比,对海表面拖曳系数和风廓线的影响非常小。当风速高于25米/秒时,海洋飞沫通过飞沫应力对海-气界面应力的作用远高于波致应力,以至于波致应力可以忽略。海表面拖曳系数在高风速下,随着风速的增大而减小。通过采用风浪谱的不同波龄,得到海洋飞沫的产生会导致海-气边界层风速的增加。最后,理论解与现场的观察数据进行了对比。对比后的数据表明,在中高风速下,飞沫对海-气边界层的影响远大于表面风浪。  相似文献
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