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对虾体内氯霉素含量测定方法的研究 总被引：1，自引：0，他引：1
应用ＨＰＬＣ技术建立常用药物氯霉素的虾体动力学研究方法。该方法的特点是：与微生物法、比色法、气相色谱法、荧光法及免疫法比较．测试准确、快速且灵敏度高。应用该方法可以从虾体内的血液、肌肉、肝胰脏、肠胃中分离出被虾吸收的氯霉素，并定量测定。在１—５０μｇ／ｍｌ范围内线性关系相关系数为０．９９９５。 相似文献
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制备5种氯霉素糖基化衍生物,并对这些衍生物的抑菌活性和安全性进行初步测试。实验结果表明:1)氯霉素被糖基化后水溶性得到了明显提高;2)氯霉素糖基化衍生物时未产生抗菌活性,但经相关糖苷酶水解后,可重新表现出抗菌活性;3)小鼠急毒试验证明,氯霉素糖基化衍生物的毒副作用与原药相比显著降低。该项研究对于设计和合成类氯霉素前药开辟了新领域。 相似文献
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发光细菌法检测水产品中氯霉素体系的建立 总被引：5，自引：0，他引：5
从青岛近海分离的多株发光细菌中筛选出1株对氯霉素敏感的鳆发光杆菌Photobacterium leiognathi YL,接种单菌落快速培养,制备出适宜检测的菌悬液,添加不同浓度的氯霉素溶液,研究氯霉素浓度与细菌发光强度抑制率之间的关系.通过控制菌体起始发光强度、菌液与氯霉素作用时间,建立发光细菌检测水产品中氯霉素体系.结果表明,当菌体的起始发光强度控制在(2.0～4.0)×105、菌液与氯霉素的最佳作用时间为30 min时,氯霉素浓度与细菌发光强度抑制率呈良好的线性关系.该方法的线性范围为0.1～1.0 ng/mL,相关系数R2为0.989,该体系对氯霉素的检测灵敏度可以达到0.1 ng/mL,可以作为水产品中痕量氯霉素残留的1种快速、灵敏的检测方法.在水产品的氯霉素加标回收试验中,鱼肉中添加浓度为0.1～1.0 ng/g时,回收率在40.34%～114.26%之间. 相似文献
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Xuanwen LU Xueqin TAO Zhi DANG 《中国地球化学学报》2006,25(B08):115-116
Chloramphenicol （CAP） is an antibiotic commonly used in aquaculture as a prophylactic or disinfectant to prevent diseases, or as a chemotherapeutic agent to control diseases. This antibiotic is either spread directly in the aquatic environment or administered through medicated feeds. It may be directly exposed to or enter the environment by leaching from uneaten feeds or from the aquatic animals＇ excrements. The antibiotic also directly enters the environment through pharmaceutical wastewater. Toxicity of CAP to human bone marrow has been linked to fatal aplastic anemia. Antimicrobial residues enter the environment, which may also establish a selective pressure in favour of antimicrobial-resistant bacteria. The residues of antibiotics may pose potential risk to public health or increase the occurrence of antibiotic-resistant bacteria in the aquatic environment. Due to these perceived risk, treatment of CAP in farm animals intended for human consumption has been restricted. However, residue of CAP in the environment is still a concern. There have been some reports on the residual effects of CAP on aquaculture and on microbial resistance to CAP in certain environments. The biotransformation of CAP in aquaculture pond sediments has been reported, which indicated that salinity is one of the many environmental factors that affect the degradation of CAP. However, acclimatization and screening of dominant bacteria for biodegrading CAP have been rarely studied. This paper studied the microbial degradation of CAP. In order to screen the dominant bacteria for degrading, the source of bacteria was sampled from the aquaculture pond sediments of Baiyun Borough, Guangzhou, China. 60 mg/L CAP was used as a sole carbon source to screen CAP-degrading bacteria and the dominant bacteria were acclimated after 2 months. 相似文献
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