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参对光照变化非常敏感，研究刺参对光照的分子响应非常重要。本研究应用RNA测序获取了刺参暴露于强光（“强光”）、正常光照（“对照”）和完全黑暗（“黑暗”）环境下体壁的基因表达谱情况，通过“对照”与“黑暗”，“对照”与“强光”和“黑暗”与“强光”的比较，在|log2 ratio|≥1和FDR≤0.001的标准下分别发现了1161、113和1705个差异表达基因（DEGs）。基因本体分析表明，“cellular process”和“binding”在“生物过程”和“分子功能”类别中的DEGs富集最多，而“cell”和“cell part”在“细胞组分”类别中的DEGs富集最多。将DEGs与Kyoto Encyclopedia基因和基因组数据库上的于214、41和229条通路进行比对，发现了51、2和57条通路分别显著富集。本研究发现的光特异性DEGs可作为深入研究刺参对光照变化的生化适应机制的重要目标基因。 相似文献
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为探究不同产地浒苔型饵料对幼刺参生长、消化和非特异性免疫的影响,本实验将青岛浒苔与宁波浒苔的干粉与海泥分别按一定质量比例混合,开展刺参饲喂实验,并与刺参天然饵料马尾藻进行对比。结果表明,青岛浒苔饵料和马尾藻饵料喂养的刺参的粗蛋白含量分别为14.31%±0.10%和15.43%±1.41%,显著高于宁波浒苔饵料(11.17%±0.63%),粗脂肪和灰分含量无显著差异;青岛浒苔组、宁波浒苔组和马尾藻组的增重率分别为22.65%±5.68%、3.03%±1.17%和20.47%±2.01%,特定生长率分别为(1.44±0.33)、(0.21±0.08)、(1.33±0.12)%/d,青岛浒苔组和马尾藻组刺参的增重率和特定生长率显著高于宁波浒苔组;青岛浒苔组、宁波浒苔组和马尾藻组刺参肠道淀粉酶、胃蛋白酶、胰蛋白酶和纤维素酶活力无显著差异;马尾藻组刺参体腔液碱性磷酸酶活力为(17.57±4.56)金氏单位/100mL,显著高于青岛浒苔组[(5.56±1.32)金氏单位/100mL]和宁波浒苔组[(2.83±0.75)金氏单位/100mL],超氧化物歧化酶和酸性磷酸酶活力无显著差异。由此可见,绿潮暴发时,通过打捞浒苔用以配制刺参饵料,既有助于缓解绿潮的生态灾害,又能够补充刺参饵料来源,具有广阔的生态效益和市场前景,但是其营养成分影响因素较为复杂,配制饵料时应充分考虑不同品种、采集时间和生长地点的差异,并通过一些前处理手段充分发挥浒苔的饵料价值。 相似文献
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Emergy analysis is effective for analyzing ecological economic systems. However, the accuracy of the approach is af-fected by the diversity of economic level, meteorological and hydrological parameters... 相似文献
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紫外线照射使精子遗传物质失活,用细胞松弛素B(CB)处理受精卵抑制第二极体释放,诱导刺参(Apostichopusjaponicus)雌核发育二倍体。用强度为2561μW/(cm2.s)的紫外线(254 nm)照射不同时间的精子与正常卵子受精。结果发现随照射时间的增加,卵裂率、早期胚胎存活率和小耳幼虫发生率逐渐降低,照射30 s时小耳幼虫发生率降为0。受精后35 min,显微镜下观察到有20%~30%受精卵排出第1极体时,用浓度0.5μg/mL的CB持续处理受精卵20 min,诱导出刺参第二极体抑制型雌核发育二倍体,雌核发育二倍体发育速度低于正常二倍体。微卫星分析表明,雌核发育二倍体诱导成功率为93.3%。 相似文献
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The sea cucumber Apostichopus japonicus is a commercially and ecologically important species in China. A total of 3056 potential unigenes were generated after assembling 7597 A. japonicus expressed sequence tags (ESTs) downloaded from Gen-Bank. Two hundred and fifty microsatellite-containing ESTs (8.18%) and 299 simple sequence repeats (SSRs) were detected. The average density of SSRs was 1 per 7.403 kb of EST after redundancy elimination. Di-nucleotide repeat motifs appeared to be the most abundant type with a percentage of 69.90%. Of the 126 primer pairs designed, 90 amplified the expected products and 43 showed polymorphism in 30 individuals tested. The number of alleles per locus ranged from 2 to 26 with an average of 7.0 alleles, and the observed and expected heterozygosities varied from 0.067 to 1.000 and from 0.066 to 0.959, respectively. These new EST-derived microsatellite markers would provide sufficient polymorphism for population genetic studies and genome mapping of this sea cucumber species. 相似文献
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Yancui Zhao Kangsen Mai Wei Xu Wenbing Zhang Qinghui Ai Yanjiao Zhang Xiaojie Wang Zhiguo Liufu 《中国海洋大学学报(英文版)》2011,10(3):293-300
The effects of probiotic Bacillus TC22 (isolated from intestine of infected sea cucumber) and prebiotic fructooligosaccharide (FOS) on growth, immunity and disease resistance in sea cucumber Apostichopus japonicus were studied. Six experimental diets were formulated with combinations of three levels of TC22 (0, 107 and 109 CFU g-1 diet) and two levels of FOS (0 and 0.5%) in a 3 × 2 factorial experiment. At the end of the 8-week feeding trial,animals were challenged by injecting Vibrio splendidus. The results revealed that the specific growth rates (SGR) of sea cucumbers were not affected by TC22 and FOS, or the interaction between TC22 and FOS (P > 0.05). However,there were significant interactions between TC22 and FOS for immune response and disease resistance in sea cucumbers (P < 0.05). When sea cucumbers were fed with TC22 at 109 CFU g?1 feed and 0.5% FOS alone or in combination, the phagocytosis, respiratory burst and phenoloxidase activity of sea cucumber coelomocytes were significantly enhanced;the disease resistance against V. splendidus infection was also increased significantly (P < 0.05).However, dietary combination of TC22 at 109 CFU g-1 diet and 0.5% FOS did not yield significantly higher efficiency than either TC22 at 109 CFU g-1 diet or 0.5% FOS used alone (P > 0.05). Therefore, further studies should examine the effects of combinations of other levels of FOS(> 0.5% or < 0.5%) and TC22 on the immunity and disease resistance of sea cucumbers. 相似文献
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在实验室条件下,研究了全光照(24L︰0D)、半光照半黑暗(12L︰12D)、全黑暗(0L︰24D)3种光照周期下刺参(Apostichopus japonicus)的摄食节律、摄食率以及3种消化酶(脂肪酶、淀粉酶、胰蛋白酶)活力的昼夜变化规律。结果显示:(1)在全光照条件下,刺参极少活动,24 h内的摄食比例较低(6%~10%);在半光照半黑暗条件下,刺参光照阶段运动不活跃,摄食比例较低(0~10%),黑暗阶段运动极其活跃,摄食比例较高;在全黑暗条件下,刺参一直处于较活跃状态,24 h内的摄食比例都较高。3种光照条件下,刺参的摄食高峰都出现在00:00~02:00,且刺参夜晚的摄食比例显著高于白天(P0.05);(2)刺参的日摄食率从全光照、半光照半黑暗到全黑暗依次增加,3种光照周期条件下刺参夜晚的摄食率极显著高于白天的摄食率(P0.01);(3)3种光照周期下,刺参3种消化酶活力的最大值都出现在22:00~02:00,消化酶活力的最大值比摄食高峰早0~4 h。研究结果表明,光照对刺参的摄食节律和消化酶活力昼夜变化都有显著影响,刺参具有提前分泌消化酶为即将到来的摄食做好准备的调节机制。 相似文献
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仿刺参(Apostichopus japonicus Selenka)的体色变化很大,多数背腹均为黄褐色,极少数呈白化特征,背腹均为纯白色。经人工繁育实验发现,白化仿刺参的子代仍多具白化特征。本文研究了虾青素基因与刺参白化特征的相关性。在克隆虾青素基因cDNA全长的基础上,比较了普通仿刺参和白化仿刺参在不同发育时期虾青素基因表达量的差异。结果表明,该基因的cDNA含有2058个核苷酸,编码560个氨基酸。经实时定量PCR分析,白化成参体壁中虾青素基因表达量显著低于普通成参;而在仿刺参色素沉积的早期,白化稚参体壁中虾青素基因表达量从受精后第39天开始显著低于普通稚参。可见,仿刺参体壁中虾青素基因的低表达与刺参白化特征的发生密切相关。 相似文献