排序方式： 共有3条查询结果，搜索用时 15 毫秒
1
1.
通过荧光染色、稀释培养、生理生化和16SrRNA基因鉴定等方法,研究了北极黄河站附近海域海水中浮游细菌的丰度、可培养浮游细菌形态学特征及多样性;采用高通量测序技术研究了黄河站附近采样站点(BJ2)的不可培养浮游细菌群落结构。可培养研究结果表明:(1)9个站点(BJ1-BJ9)水样平均含菌数为2.88×108个/L;(2)21株可培养浮游细菌中,仅AB08、AB09和AB17三株细菌为革兰氏阳性菌;(3)细菌主要包括放线菌纲(Actinobacteria),黄杆菌纲(Flavobacteriia)和γ-变形菌纲(Gammaproteobacteria),其中假单胞菌属(Pseudomonas)为优势菌(占71%)。高通量测序结果显示:在得到的1 467个不可培养浮游细菌OTU中,变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes)为优势菌群,所占比例分别为49.71%和41.61%,还有少量的浮霉菌门(Planctomycetes)和疣微菌门(Verrucomicrobia);其中变形菌门中以α-变形菌纲(α-Proteobacteria)为主要类群。 相似文献
2.
自然环境溶解有机碳（DOC）的组成影响着微生物群落结构,不同微生物类群对有机碳源类型的利用表现出差异。然而,纯菌株的碳源利用选择性及其和盐度的关系却仍不是很清楚。本文采用单碳源纯培养技术、聚合酶链式反应（PCR,Polymerase Chain Reaction）和相关统计方法,研究了青藏高原北部六个湖泊（洱海、青海湖、托素湖、尕海1、尕海2、小柴旦湖）沉积物中的基于不同碳源可培养细菌多样性及其与盐度的响应关系。采用七种不同类型的单碳源（甲酸钠、乙酸钠、丙酮酸钠、乳酸钠、葡萄糖、纤维素、混合氨基酸）进行培养筛选。本文共获得10个细菌分类属,它们分属于假单胞菌属（Pseudomonas）、气单胞菌属（Aeromonas）、希瓦氏菌属（Shewanella）、脱氯单胞菌属（Dechloromonas）、动性球菌属（Planococcus）、盐单胞菌属（Halomonas）、微小杆菌属（Exiguobacterium）、弧菌属（Vibrio）、交替假单胞菌属（Pseudoalteromonas）和芽孢杆菌属（Bacillus）。其中,盐单胞菌属菌株可利用本研究使用的所有碳源类型,说明这类细菌具有广泛碳代谢途径,并潜在地参与了青藏高原北部湖泊碳循环过程。所得的纯菌株利用不同碳链长度的碳源具有选择性,即随着盐度增高,某些菌株更偏向利用结构更加复杂的碳源。总之,盐度不仅影响着纯培养细菌群落多样性,而且还影响着细菌对碳源类型的选择。 相似文献
3.
Liang Shen Tandong Yao Baiqing Xu Hongmei Wang Nianzhi Jiao Shichang Kang Xiaobo Liu Yongqin Liu 《地学前缘(英文版)》2012,3(3):327-334
Ice melt water from a 22.27 m ice core which was drilled from the East Rongbuk Glacier, Mt.Everest was incubation in two incubation ways:plate melt water directly and enrichment melt water prior plate,respectively.The abundance of cultivable bacteria ranged from 0-295 CFU mL-1 to 0-1720 CFU mL-1 in two incubations with a total of 1385 isolates obtained.Comparing to direct cultivation, enrichment cultivation recovered more bacteria.Pigment-producing bacteria accounted for an average of 84.9%of total isolates.Such high percentage suggested that pigment production may be an adaptive physiological feature for the bacteria in ice core to cope with strong ultraviolet radiation on the glacier.The abundances of cultivable bacteria and pigment-producing isolates varied synchronously along deplh:higher abundance in the middle and lower at the top and bottom.It indicated that the middle part of the ice core was hospitable for the microbial survival.Based on the physiological properties of the colonies,eighty-nine isolates were selected for phylogenetic analysis.Obtained 16S rRNA gene sequences fell into four groups:Firmicutes,Alpha-Proteobacteria,Gamma-Proteobacteria, and Actinobacteria,with the Firmicutes being dominant.Microbial compositions derived from direct and enrichment cultivations were not overlapped.We suggest that it is a better way to explore the cultiiiable microbial diversity in ice core by combining the approaches of both direct and enrichment cultivation. 相似文献
1