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1.
根据NCBI数据库中已发表的溶藻弧菌(Vibrio alginolyticus)全基因组序列合成一对特异性引物,应用聚合酶链式反应(PCR技术)扩增溶藻弧菌HY9001菌株dtd基因,将其定向克隆到原核表达载体pET-32a构建重组表达质粒pET-DTD,并对其进行诱导温度、诱导时间、诱导IPTG浓度等条件的优化,最后探究其纯化时最佳咪唑洗脱浓度。结果表明:DTD蛋白成功表达,且其以包涵体的形式存在,在诱导温度37℃、IPTG浓度0.1 mmol/L条件下诱导5 h表达量最高,纯化最佳咪唑洗脱浓度为150 mmol/L。 相似文献
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New empirical models were developed to predict the soil deformation moduli using gene expression programming (GEP). The principal soil deformation parameters formulated were secant (Es) and reloading (Er) moduli. The proposed models relate Es and Er obtained from plate load-settlement curves to the basic soil physical properties. The best GEP models were selected after developing and controlling several models with different combinations of the influencing parameters. The experimental database used for developing the models was established upon a series of plate load tests conducted on different soil types at depths of 1–24 m. To verify the applicability of the derived models, they were employed to estimate the soil moduli of a part of test results that were not included in the analysis. The external validation of the models was further verified using several statistical criteria recommended by researchers. A sensitivity analysis was carried out to determine the contributions of the parameters affecting Es and Er. The proposed models give precise estimates of the soil deformation moduli. The Es prediction model provides considerably better results in comparison with the model developed for Er. The simplified formulation for Es significantly outperforms the empirical equations found in the literature. The derived models can reliably be employed for pre-design purposes. 相似文献
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天津市地下水开采对地面沉降影响的多元回归分析 总被引:1,自引:0,他引:1
地下水的过量开采是天津市引起地面沉降的主要原因。因此天津市提出了“压缩地下水开采量”、“地下水人工回灌”、“调整地下水开采层次”等控制地面沉降的3大技术措施。经过多年的努力,控制地面沉降效果明显。如何解决地下水开发与控制地面沉降的关系,更好的贯彻这3大技术措施,是该文编写的初衷。即在开采同样地下水量的情况下,如何使地面沉降量最小;或在地面沉降量容许的情况下,如何开采最大量的地下水。压缩地下水开采量是治理地面沉降的根本措施,亦即如何压缩采水量或调整开采层次会达到最好效果。论文对天津市某区各个地下水开采层的多年累计开采量、累计沉降量进行数据统计分析,建立了该地区累计沉降量及各个地下水开采层的多元相关方程。在此基础上,分析了各个地下水开采层对地面沉降影响的相关程度。以此为该区控制地面沉降的提供依据。 相似文献
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采用随机克隆、功能筛选、逐次排除和同源比较的基因克隆新策略进行克隆假单胞菌(Pseudomonas sp.cn4902)磷酸甘油磷酸酯酶基因的研究。结果表明,该结构基因长819bp,与铜绿假单胞菌的磷酸甘油磷酸酯酶基因的核苷酸一致性达61.5%,氨基酸同源性为56.2%。该基因已输入GenBank数据库,收录号AF348165。将该基因转化大肠杆菌,受体菌在含NaCl 1.0mol/L的培养基中甘油含量升高2.9倍,最终菌浓度提高3.6倍。可见这是一个与生物耐盐性相关的主基因,以其转化、培育耐盐农作物的前景十分光明。 相似文献
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沿海经济发达地区农村居民点整理适宜性研究——以福清市为例 总被引:9,自引:0,他引:9
以福建省沿海经济较发达的福清市为例,通过对样本区域农村居民点用地的现状进行调查,并在调查数据的基础上测算出农村居民点用地的整理潜力,以此为基础,选取福清市土地利用现状、自然因素、社会因素、经济因素等方面12个指标,采用因子分析和聚类分析方法对农村居民点整理的适宜性进行评价,根据聚类结果将18个评价单元划分为4个不同的适宜性类型区,结合实际情况和定量分析过程提出各类型区的整理途径和整理对策. 相似文献
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A cDNA microarray technique applied for analysis of global gene expression profiles in tributyltin-exposed ascidians 总被引:2,自引:0,他引:2
To analyze global gene expressions, we constructed a cDNA microarray from a basal chordate, the ascidian Ciona intestinalis. Ciona is a cosmopolitan species and a genomic analysis of Ciona revealed that ascidians had approximately 15,500 protein-coding genes. Our "Ciona intestinalis cDNA chip version 1 (Ci cDNA chip ver. 1)" has arrayed 13,400 unique Ciona cDNAs. To establish a detection system for gene expression profiles in wild ascidians using a cDNA microarray, we analyzed gene expressions in the whole body of Ciona adults after exposure to 100 nM tributyltin (TBT) for 24 h. In our preliminary array data using Ci cDNA chip ver. 1, we found more than 200 genes that showed strong differential expressions. These genes encoded proteins that were concerned with stress response, detoxification, oxidoreduction reaction, biosynthesis, and catabolism. This, the first large cDNA microarray of this animal, should facilitate analyses of global gene expressions following exposure to TBT. 相似文献
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正1 Introduction Many soda and salt lakes are characterized by the formation of the meromictic conditions under which a part of the water column is not involved in the annual process of mixing(Mac Intyre,Melack,1982).This creates an 相似文献
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Slawomir Jack Giletycz Andrew Tien-Shun Lin Katsura Yamada Liang-Chi Wang Chih-Wei Chien Jiann-Yuh Lou Neng-Wei Huang Che-Yung Ting Kuo-Wei Shih 《地球表面变化过程与地形》2021,46(12):2379-2394
Newly emerged landscapes above sea level are characterized by rapidly evolving geomorphic systems where the initial fluvial pattern adapts to a former submarine topography. Such an early formed fluvial system establishes drainage basins and unstable landforms that characterize high topographic asymmetry which are prone to fast removal or reorganization. Transitional landscapes might form depositional systems as lakes or ponds that subsequently are incised, captured and incorporated into drainage basins. In this study we focus on the recently emerged Hengchun Peninsula to survey its paleoenvironment evolution. Three drillings performed in the Gangkou basin with fieldwork revealed several indicators that reconstructed stages of the landscape reorganization. The major finding shows an ephemeral large lake in the central part of the Hengchun Peninsula that was drained to the Pacific c. 6000 bp . The lake belonged to an ephemeral lakeland that was created after the emergence of the peninsula. Currently, several areas as relict landforms indicate this stage of topography evolution that through high rates of incision and subsequent captures, transforms into drainage basins. Furthermore, two drillings show brackish waters at the present estuary of the Gangkou basin. These two different paleoenvironments today build one system – Gangkou catchment. Long-term uplift rates show that a hanging wall of the Hengchun Fault plays a significant role in the creation of a lakeland by tilting the peninsula's surface. The tilt impacts on asymmetrical emergence of the peninsula and catchment development. Our study shows that a new geomorphic system might create depositional ephemeral landforms (lakes) that represent phases of early topography evolution after emergence above a sea level that are subjected to instantaneous rearrangement and evolves through large-scale phases before it reaches a topographic steady-state. 相似文献